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Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo

Keanekaragaman Echinodermata dan Hubungannya dengan Komposisi Lamun pada Zona Intertidal Pantai Kiama, Sulawesi Utara Julianti Malensang; Revolson A. Mege; Nonny Manampiring
Switch : Jurnal Sains dan Teknologi Informasi Vol. 3 No. 5 (2025): September : Switch : Jurnal Sains dan Teknologi Informasi
Publisher : Asosiasi Profesi Telekomunikasi Dan Informatika Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/switch.v3i5.686

Seagrass beds are coastal ecosystems that have important ecological functions as a provider of habitat, food sources, protection, and reproductive areas for various marine life including Echinoderms. This study aims to analyze the diversity of Echinoderms and their relationship with seagrass distribution in Kiama Beach, Melonguane District, Talaud Islands Regency. The research was conducted in the intertidal zone at three stations using the exploration method with purposive sampling technique. Each station is divided into three plot installation zones measuring 4 × 4 meters, with observations made at low tide. Environmental parameters (temperature and pH), type and number of Echinoderm individuals, and seagrass species were recorded and analyzed using the Shannon-Wiener diversity index and product moment correlations. The results showed that the Echinoderma community consists of two classes and five species: Archaster typicus, Linckia laevigata, Culcita novagueniae, Holothuroidea scabra, and Holothuroidea forskali, with the Asteroidea class dominating (75%). The diversity index (H') value was relatively low at all stations with a range of 0.6261–0.6631. Seagrass beds consist of five species, namely Halophila ovalis, Cymodocea rotundata, Thalassia hemprichii, Halodule uninervis, and Syringodium isoetifolium, with the highest abundance found in Halophila ovalis. Correlation results showed a strong to very strong relationship between the abundance of Echinoderms and certain seagrass types (r = 0.77–0.99) as well as a strong correlation at the overall level of the station (r = 0.74). This study confirms that the presence and abundance of Echinoderms is greatly influenced by the density and composition of seagrass species. These findings provide important baseline data for the management and conservation of seagrass beds as a support for the sustainability of coastal biodiversity.

Identification of Bacteria Isolated from Sea Urchin (Diadema setosum) Gonads Inhibiting Pseudomonas fluorescens and Staphylococcus aureus Growth Andini Maurent Dwiyanti; Helen Joan Lawalata; Iriani Setyawati; Anita Constanci Christine Tengker; Nonny Manampiring
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 2 (2025): June 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i02.p05

Sea urchins are organisms that have antibacterial, antitumor, and anticancer abilities. The gonads can be used as a food source because they store 28 kinds of amino acids, vitamin B complex. This research aims to determine the type of bacteria from the gonads of sea urchins (Diadema setosum) which have inhibitory power against the growth of Pseudomonas fluorescens and Staphylococcus aureus bacteria. This research used an exploratory method which was analyzed descriptively, using stages in which sea urchin samples were taken and antibacterial tests against Pseudomonas fluorescens and Staphylococcus aureus from sea urchin gonads using the spread method. The media used for the process of isolating bacteria from the gonads of the sea urchin Diadema setosum is Nutrient Agar (NA) media. Isolate colonies that grew after incubation for 24 hours were separated based on differences in colony morphology. Morphological characterization of bacterial cells begins with gram staining. The diameter of the inhibition zone in the test bacteria shows different shapes. The research obtained 8 bacterial isolates, namely isolates B1-B8 which were gram-negative. The eight bacterial isolates have the potential as antibacterials against the test bacteria P. fluorescens and S. aureus. Isolates B1, B2, B3, B7 are similar to the genus Proteus, while isolates B4, B5, B6, and B8 are similar to the genus Citrobacter. The diameter of the largest inhibition zone in the P.fluorescens test bacteria, namely isolate B1, was 9.03 mm. Meanwhile, the smallest diameter for isolate B7 was 6.97 mm. In the S.aureus test bacteria, the largest diameter in isolate B1 was 11.09 mm. Meanwhile, the smallest diameter for isolate B4 was 8.57 mm.

Efektivitas Serbuk Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera) terhadap Kadar Glukosa Darah Tikus Putih (Rattus norvegicus L.) Hiperglikemia Elsi Elsi; Iriani Setyawati; Nonny Manampiring; Yermia Semuel Mokosuli; Anita Constanci Christine Tengker; Helen Joan Lawalata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p6

Dua puluh tujuh ekor tikus putih (Rattus norvegicus L.) jantan dibagi menjadi 9 kelompok perlakuan yaitu kontrol normal (K1), kontrol negatif (K2), kontrol positif (K3), serbuk daun kersen 800 mg/kgB (K4), serbuk daun kelor 800 mg/kgBB (K5), serbuk daun kersen 1600 mg/kg BB (K6), serbuk daun kelor 1600 mg/kgBB (K7), kombinasi serbuk daun kersen 400 mg/kgBB dan daun kelor 400 mg/kg BB (K8), kombinasi serbuk daun kersen 800 mg/kg BB dan daun kelor 800 mg/kgBB (K9). Pengukuran kadar glukosa darah tikus dilakukan pada hari ke-3, 7, dan 10. Hasil analisis menunjukan bahwa pemberian dosis kombinasi serbuk daun kersen 800 mg/kgBB dan daun kelor 800 mg/kgBB merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah pada tikus putih jantan

Efektivitas Serbuk Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera) terhadap Kadar Glukosa Darah Tikus Putih (Rattus norvegicus L.) Hiperglikemia Elsi Elsi; Iriani Setyawati; Nonny Manampiring; Yermia Semuel Mokosuli; Anita Constanci Christine Tengker; Helen Joan Lawalata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p6

Dua puluh tujuh ekor tikus putih (Rattus norvegicus L.) jantan dibagi menjadi 9 kelompok perlakuan yaitu kontrol normal (K1), kontrol negatif (K2), kontrol positif (K3), serbuk daun kersen 800 mg/kgB (K4), serbuk daun kelor 800 mg/kgBB (K5), serbuk daun kersen 1600 mg/kg BB (K6), serbuk daun kelor 1600 mg/kgBB (K7), kombinasi serbuk daun kersen 400 mg/kgBB dan daun kelor 400 mg/kg BB (K8), kombinasi serbuk daun kersen 800 mg/kg BB dan daun kelor 800 mg/kgBB (K9). Pengukuran kadar glukosa darah tikus dilakukan pada hari ke-3, 7, dan 10. Hasil analisis menunjukan bahwa pemberian dosis kombinasi serbuk daun kersen 800 mg/kgBB dan daun kelor 800 mg/kgBB merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah pada tikus putih jantan

EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.

EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.

Pengembangan Modul Berbasis Riset Keanekaragaman Spesies Ikan di Danau Tondano terhadap Literasi Sains Mahasiswa Pendidikan Biologi Manampiring, Nonny; Mamahit, Jimmi Andrew; Posumah, Dany Ch.
SCIENING : Science Learning Journal Vol. 4 No. 1: Juni 2023
Publisher : Jurusan Pendidikan IPA FMIPAK Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/slj.v4i1.6629

Penelitian ini bertujuan untuk mengembangkan produk berupa modul pembelajaran berbasis hasil riset keanekaragaman spesies ikan di danau Tondano pada mata kuliah Biodiversitas Sulawesi Penelitian ini menggunakan jenis penelitian dengan desain pengembangan ADDIE analyze design development implementation evaluation Pengembangan modul ajar ini dilakukan dalam menjawab permasalahan terhadap kendala mahasiswa dalam memperoleh sumber referensi belajar yang lebih spesifik dikarenakan pada mata kuliah tersebut belum melakukan pengembangan tentang biodiversitas fauna khususnya keanekaragaman ikan di daerah danau Tondano Modul yang dikembangkan melaui penyusunan storyboard terlebih dahulu untuk memudahkan menyusun produk akhir Modul hasil pengembangan terlebih dahulu divalidasi oleh ahli materi dengan hasil validasi sebesar 97 48 dengan kategori sangat valid dan validasi ahli media dengan hasil validasi sebesar 93 90 dengan kategori sangat valid Uji efektivitas modul terhadap literasi sains dengan perolehan nilai N Gain 87 70 dengan kategori sangat efektif dan pembagian penskoran memperoleh nilai N Gain 0 88 dengan kategori tinggi Hasil respon mahasiswa terhadap modul menunjukkan nilai sebesar 95 61 dengan kategori sangat praktis < p>

Effectiveness of Sea Cucumber (Holothuria atra) Extract Ointment from the Talaud Islands on Burn Wound Healing in Mice (Mus musculus) Mumu, Trivena Vannesa; Roring, Verawati Ida Yani; Manampiring, Nonny
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p061-070

Sea cucumber (Holothuria atra) has been traditionally used for wound treatment; however, scientific evidence supporting its effectiveness in burn wound healing remains limited. This study presents a novel investigation of the wound healing potential of H. atra extract sourced specifically from the Talaud Islands and formulated into multiple topical ointment concentrations for second-degree burn treatment. The study aimed to evaluate the effectiveness of H. atra extract ointment in accelerating second degree burn wound healing in mice. The extract was obtained by maceration using 95% ethanol and formulated into ointments at concentrations of 10%, 15%, and 20%. Twenty-five male mice were randomly assigned to five groups: positive control (1% silver sulfadiazine), negative control, and three treatment groups receiving H. atra extract ointments at different concentrations. Parameters observed included wound diameter reduction, physical characteristics of the ointment, phytochemical screening, and pain-related behavioral responses. The results demonstrated that the 15% H. atra extract ointment produced the highest wound healing effectiveness, with a wound contraction rate of 96% on day 14, followed by the 10% (84%) and 20% (75%) formulations. Phytochemical analysis revealed the presence of flavonoids, saponins, steroids, and alkaloids, which are associated with anti-inflammatory, antimicrobial, and tissue regenerative activities. The ointment formulations met homogeneity and organoleptic requirements, although their spreadability values were below the recommended standard. These findings highlight the novelty of identifying an optimal concentration (15%) of H. atra extract ointment that provides superior burn wound healing activity compared with other tested concentrations. In conclusion, the 15% H. atra extract ointment shows potential as an alternative topical therapy for second-degree burn wounds.

Efficacy and Physiological Responses of Payangka Fish (Ophieleotris aporos) to Anesthetization with Clove Oil Livana Dethris Rawung; Wendy Alexander Tanod; Nonny Manampiring
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.4726

This study aims to determine the effect of clove oil concentration and time of immersion in clove oil on the anesthesia, recovery time, and physiological responses in the payangka fish (Ophieleotris aporos). The experimental design was completely randomized with a 6 x 3 factorial arrangement. The first factor was a concentration of clove oil consisting of six levels i.e., 0.02; 0.03; 0.04; 0.05; 0.1, and 0.2 ml/L of water. The second factor was a time of immersion consisting of three levels i.e., 1; 3; and 6.5 hours. Results showed that the concentration of clove oil significantly (p<0.05) affected the anesthesia time and the recovery time after immersing. The length of immersing and concentration of clove oil also significantly (p<0.05) affected the concentration of glucose, but not significantly (p>0.05) affected the concentration of cholesterol. It showed that clove oil can be used as anesthetization for payangka fish in the appropriate dose. The optimal concentration of clove oil that can be used as anesthesia for the transportation of live payangka fish is 0.02 ml/L of water. Meanwhile, for handling activities on payangka fish, a clove oil concentration of 0.03 ml/L of water is recommended

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