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All Journal Jurnal Ilmiah Poli Rekayasa MEDISAINS Dinamika Teknik Mesin : Jurnal Keilmuan dan Terapan Teknik Mesin Jurnal Ilmu dan Teknologi Kesehatan Journal of Health Sciences Jurnal Analis Medika Biosains (JAMBS) Borneo Journal of Medical Laboratory Technology Oryza : Jurnal Pendidikan Biologi JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Prosiding University Research Colloquium Jurnal Karya Pengabdian Teknik Mesin Energy, Materials and Product design JMLS: Journal of Medical Laboratory and Science Journal of Medical Laboratory Research Indonesian Journal of Electronics, Electromedical Engineering, and Medical Informatics Ulil Albab
Arif Mulyanto, Arif
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Journal : Borneo Journal of Medical Laboratory Technology

 1 
Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae: Preparation of Coconut Shell Charcoal Gel and Inhibitory Test Against Klebsiella pneumoniae Bacteria Kamalah, Siti Rahmah; Kurniawan, Kurniawan; Mulyanto, Arif; Dhanti, Kurnia Ritma
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6082

Abstract

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.
Uji Efektifitas Ekstrak Kulit Pisang Kepok (Musa acuminata balbisian colla) dan Daun Salam (Syzygium polyanthum) Sebagai Antibakteri Terhadap Pseudomonas aeruginosa: Test the Effectiveness of Banana Peel Extract Kepok (Musa acuminata balbisian colla) and Bay Leaves (Syzygium polyanthum) as an Antibacterial Pseudomonas aeruginosa Putranti, Respatiningtyas Amadea Danfi; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Mulyanto, Arif
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.8576

Abstract

Pseudomonas aeruginosa merupakan bakteri Gram negatif dari keluarga Pseudomonasadaceae dan terkenal dengan ketahananya yang mampu beradaptasi diberbagai lingkungan, suhu, bersifat aerob dengan mengoksidasi substrat untuk memperoleh energi, dapat bergerak menggunakan flagel dan tahan terhadap berbagai jenis antibiotik. Salah satu tindakan yang dapat dilakukan untuk mengurangi resistensi antibiotik yaitu memanfaatkan tanaman yang berkhasiat sebagai obat. Tujuan dari penelitian ini adalah untuk menguji efektifitas antibakteri ekstrak kulit pisang kepok (Musa acuminata balbisian colla) dan ekstrak daun salam (Syzygium polyanthum) terhadap pertumbuhan bakteri Pseudomonas aeruginosa. Jenis penelitian yang digunakan yaitu true eksperimental post test only with control group design. Metode yang digunakan difusi cakram menggunakan media Nutrient Agar (NA), sampel dibagi menjadi tiga kelompok dengan perbandingan konsentrasi ekstrak kulit pisang kapok dan daun salam (F1 25%:75%) , (F2 50%:50%) , (F3 75%:25%), kontrol positif (kloramfenikol 3ยต), kontrol negatif (DMSO 5%), pengulangan dilakukan sebanyak 3 kali. Hasil pengujian yang telah dilakukan menunjukan pembentukan zona hambat pada ketiga perlakuan dengan hasil rata-rata F1 5,2 mm , F2 4,1 mm, F3 3,2 mm. Zona hambat terbesar terbentuk pada K+ , pada kelompok perlakuan zona hambat terbesar yaitu F1 kategori zona hambat lemah sampai sedang yang artinya kelompok perlakuan tidak efektif dalam menghentikan penyebaran bakteri Pseudomonas aeruginosa.
Co-Authors Abdul Latif Achmad Zainuri Anjani, Annisa Gallela Brilianto, Samuel Surya Dhanti, Kurnia Ritma Diniatik Dita Pratiwi Kusuma Wardani Emmy Dyah Sulistyowati, Emmy Dyah Farid Ishartomo Fitriyani gema romadhona Hanifa, Noha Harisman Jaya, Harisman I Kade Wiratama I Kade Wiratama, I Kade I Made Mara I.G.B. Dipa Maha Putra, I.G.B. Dipa Maha IDK Okariawan, IDK Ikhsan Mujahid Iwan Setiawan Jumrianto, Jumrianto Kamalah, Siti Rahmah Khasanah, Tyas Ulfah Kurniawan Kurniawan Kusnanto Mukti Wibowo KUSUMA WARDANI Lalu Wahyu Alam Liana Anggraeni, Liana M. Athar, M. M. Mirmanto, M. Made Mara, Made Muhamad Ali Imron MUJIONO, TIMBUL Muliadi Harianhady, Muliadi N. Prasath5, N. Prasath Nafisah, Zhafirah Shafa Kamalia Nasmi Herlina Sari nur laela NURCHAYATI Nurchayati Nurchayati Nurul Badriyah Nuryaningsih, Ely Okariawan, I Dewa Ketut Pandri Pandiatmi Papulasih, Dian Prabowo, Finda Rizky Putri Pratiwi, Galuh Dea Nur Putranti, Respatiningtyas Amadea Danfi Putri, Ayuni - Qo'imah, Alamanda Nurul Ragil Setiyabudi, Ragil Rahaju, Minto Regawa Bayu Pamungkas RETNO SULISTYOWATI Rofiqoch, Isnaeni Romadhon, Zakiyatudin Anduko Royan , Royan Royan -, Royan Rudy Sutanto Rum Sapundani Rusman, Ade Salman Salman Salman Sinarep Sinarep Sinarep Sudarsono, Tantri Analisawati Sultan Sultan Sumantri, Indah Suci Sunarto, Presillia Grisviani Suparman Ahmadi, Suparman Syahrul Teguh Pribadi Tirta Nadi, Danu Tri Joko Raharjo Widodo, Oei Stefani Yuanita Yuliana, Ratri Nurazizah