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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Lingkungan Bioscience Edu Sains: Jurnal Pendidikan Sains dan Matematika Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Surya Medika EnviroScienteae Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Anterior Jurnal AGRIPEAT Pedagogik: Jurnal Pendidikan Jurnal Ilmiah Kanderang Tingang Jurnal Hutan Tropika Spizaetus: Jurnal Biologi dan Pendidikan Biologi Budapest International Research in Exact Sciences (BirEx Journal) Journal of Environment and Management BiosciED: Journal of Biological Science and Education International Journal of Health, Economics, and Social Sciences (IJHESS) Jurnal Pendidikan BIOMA : Jurnal Biologi dan Pembelajarannya Indonesian Journal of Advanced Social Works (DARMA)
Liswara Neneng
Universitas Palangka Raya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Transportation Other

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The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Neneng, Liswara; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.306 KB) | DOI: 10.11594/jtls.08.01.04

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Liswara Neneng; Yohanes Edy Gunawan
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.04

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
Co-Authors Abriyanto Adam, Chaidir Adi Jaya Akhmadi Akhmadi Amalia Yulianti Anita Maulina Araina, Elga Ardianoor Ardianoor Arintiana Ghazella Asri Adelia Asri Adelia Asri Pujirahadju Bambang S. Lautt Bambang Supriono Lautt Basuki, Bejo Bejo Basuki Bejo Basuki Chaidir Adam Chuchita Desimaria Panjaitan Dewi Saraswati Dicky Satriya Pratama Ebrry Dwi Putra Ennike Gusti Rahmi Fachry Abda El Rahman Fahrina, Ririn Ferayani Ferayani Ferayani, Ferayani Frans Grovy Naibaho Frenklin Yuda Laba Gamaliel Gamaliel Gunawan, Yohanes E Gunawan, Yohanes Edy Hartanti, Rio Eka Desi Purwandari Herniati , Melly Indah Sari Dewi Indri Aprilia, Indri Kusuma Putri, Rahmadyah Lautt, Bambang S. Ludang, Yetrie Lusia Widiastuti Lusia Widyastuti Meitiana Miranda, Yula Muhammad Nuruddin Naibaho, Frans Nawan Nawan Nawan Nawan Nawan, Nawan Ngazizah, Febri Nur Oksal, Efriyana Panny, Priscilla Pujirahadju, Asri Rahmad Hidayat Rahmawati, Rahmawati Ria Anjalani Rio Eka D.P. Hartanti Ruben Tinting Sirenden Sari, Nopita Tamara Sih Winarti Simatupang, Tumpal Siti Sunariyati Srininta Br Perangin-angin Stevin Carolius Angga Sulmin Gumiri Sulmin Gumiri Susilawati Susilawati Susilawati Susilawati Susilawati, Susilawati Syarpin Syarpin Tanduh, Yusintha Titin Purnaningsih Tri Widayat Tri Widayat Tumpal Simatupang Usop, Aswin Usup, Hepryandi Luwyk Djanas Vivin Alvianita Wijaya, Nuriman Y. Sulistiyanto Yetrie Ludang Yohanes Edy Gunawan Yula M Yula M, Yula Yustinus Sulistiyanto Yusurum Jagau Yusurum Jagau Zakaria Zakaria Zimon Pereiz