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Uji Efektivitas Ekstrak Batang Lengkuas (Alpinia Galanga) Terhadap Bakteri Streptococcus Pyogenes Secara In Vitro Al-Fariz, Rifqi; Kurniawan, Edy; Ustyawati, Jumari; Hijriani, Baiq Isti
JSN : Jurnal Sains Natural Vol. 2 No. 4 (2024): November
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v2i4.632

Abstract

Pyogenes merupakan bakteri patogen yang dapat menyebabkan penyakit mulai dari infeksi ringan hingga infeksi invasif yang mengancam jiwa. Penyakit yang disebabkan oleh bakteri streptococcus pyogens diobati dengan pemberian antibiotik seperti penisilin. Namun penggunaan antibiotik ini dengan dosis 500 mg dapat menimbulkan bakteri resisten akibat perubahan genetik, sehingga perlu dicari alternatif lain selain Streptococcus seperti menggunakan bahan alami yang berasal dari batang lengkuas misalnya. Batang lengkuas sendiri mengandung saponin, flaponoid, fenol yang berfungsi sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui diameter zona hambat setiap konsentrasi batang lengkuas (Alpinia galanga) terhadap bakteri Streptococcus pyogenes. Metode penelitian ini menggunakan eksperimen laboratorium, menggunakan metode difusi sumur dengan konsentrasi 100%, 75%, 50%, 25%. Hasil penelitian ini menunjukkan bahwa tidak terbentuk zona bening pada semua konsentrasi. Kesimpulan penelitian ini adalah ekstrak batang lengkuas (Alpinia galanga) tidak mampu menghambat pertumbuhan bakteri Streptococcus pyogenes.
The Effect of Sambang Darah Leaf Filtrate (Excoecaria Cochinchinensis L) to Reduce The Bleeding Time in Skin Wounds of White Rats (Rattus Norvegicus) Hijriani, Baiq Isti; Zaetun, Siti; Wiadnya, Ida Bagus Rai
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.298

Abstract

Hemostasis is the process of stopping bleeding from a damaged blood vessel. When bleeding occurs, the body will naturally respond with a hemostatic mechanism to stop the bleeding. Indonesian people have long used plants in medicine, one of which is sambang darah (Excoecaria cochinchinensis L) plant. Sambang darah has a chemical compound that is thought to stop bleeding. The purpose of this study was to determine the potential of sambang darah filtrate to reduce the bleeding time in skin wounds of white rats (Rattus norvegicus). This study is a pre-experimental study with a static group comparison approach. The number of experimental units in this study were 10 white rats which were divided into treatment group and control group. Data analysis used the Independent Sample T Test. The results showed that the average of bleeding time in the skin wounds of white rats without drops of sambang darah leaf filtrate was 252 seconds. While the average of bleeding time in the skin wounds of white rats with drops of sambang darah leaf filtrate is 163 seconds. The conclusion of this study is sambang darah (Excoecaria cochinchinensis L) leaf filtrate have a potential to reduce the bleeding time in the skin wounds of white rats (Rattus norvegicus).  
Menakar Literasi Mikrobiologi Mahasiswa Pendidikan Biologi Universitas Mataram Sakaroni, Rubiyatna; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Journal of Science and Education Research Vol. 4 No. 2 (2025): Journal of Science and Education Research
Publisher : Yayasan Pendidikan Insan Mulia Utan Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62759/jser.v4i2.316

Abstract

Microbiology literacy is an important skill for prospective biology teachers to be able to understand, teach, and apply microbiology concepts appropriately in life and learning. This study aims to describe the microbiology literacy profile of biology education students at Mataram University based on competency and attitude dimensions after completing microbiology courses. The study employs a quantitative descriptive approach using instruments developed based on the PISA 2015 science literacy framework. The research subjects were students who had taken microbiology courses. Data were analyzed descriptively to determine literacy score categories. The results showed that students' microbiology literacy was generally in the moderate to low category. In the competency dimension, the highest scores were in the ability to interpret data and scientific evidence, while the lowest scores were in the ability to evaluate and design scientific investigations. In the dimension of attitude, the majority of students were in the moderate category, with the highest scores in the assessment of scientific approaches and the lowest scores in interest in microbiology. These low scores indicate the need to strengthen higher-order thinking skills, hands-on experience in research design, and learning that links microbiology concepts to real-life contexts. This study recommends the use of project-based, problem-based, and socio-scientific issue-based learning strategies, as well as evaluations that require the application of concepts and analysis. This approach is expected to enhance students' conceptual understanding, analytical skills, and scientific attitudes in a balanced manner.
ANALYSIS OF ACTINOMYCETES ABUNDANCE IN THE RHIZOSPHERE OF MANGROVE ECOSYSTEMS IN SEKOTONG AS A POTENTIAL SOURCE OF BENEFICIAL MICROBES Sakaroni, Rubiyatna; Baiq Isti Hijriani; Wirdullutfi
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6701

Abstract

The rhizosphere of mangroves is a microhabitat rich in root exudates and organic matter, making it an ideal environment for the growth of Actinomycetes. This study aims to analyze the abundance of Actinomycetes in the mangrove rhizosphere at three different locations in Sekotong District, West Lombok: Bagek Kembar, Tanjung Batu, and Buwun Mas. Sampling was conducted using purposive sampling in the dominant root zone at a depth of ±20 cm. Actinomycetes isolation was performed using the surface plate method on SCA medium supplemented with nystatin as an antifungal agent. The results showed that the highest abundance was found in the rhizosphere of Rhizophora mucronata at Bagek Kembar with 4.6 × 10⁵ CFU/g, while the lowest was in Avicennia marina at Tanjung Batu with 1 × 10⁵ CFU/g. In general, Rhizophora mucronata showed higher abundance than Avicennia marina in all locations. This difference was influenced by root exudate characteristics, root system structure, and soil physicochemical conditions such as aeration and organic matter content. This study concludes that the rhizosphere of Rhizophora mucronata in Bagek Kembar is a more supportive habitat for the growth of Actinomycetes. This finding serves as an important basis for further exploration of Actinomycetes isolates from mangroves as potential sources of biocontrol agents and antimicrobial compound producers.
Effect of Garlic Filtrate as An Alternative Anticoagulant for Osmotic Fragility Test (OFT) Aini; Hijriani, Baiq Isti; Dewi, Fitriani Kusuma; Atfal, Bustanul
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.5623

Abstract

Osmotic fragility test (OFT) is an examination conducted to measure the resistance of erythrocytes when exposed to hypotonic saline solutions with various concentrations. The purpose of this study was to analyze the effect of garlic filtrate as an alternative anticoagulant for osmotic fragility test (OFT). The samples used were blood samples with heparin anticoagulant. This study is a pre-experimental study, using NaCl solution with concentrations of 0.2%, 0.3%, 0.35%, and 0.55%. The method used to measure OFT value is spectrophotometric method. The data obtained were analyzed using statistical one way Anova. The results showed the percentage value of hemolysis with garlic filtrate at each concentration was 100%, 99%, 95%, and 5%, respectively. Data analysis using ANOVA showed that there was a significant effect on the effect of garlic filtrate as an alternative anticoagulant on OFT with (p < 0.05) 0.000 < 0.05.
Uji Aktivitas Penghambatan Bakteri Endofit Daun Pepaya (Carica papaya L.) Terhadap Staphylococcus aureus Faujiah, Putri; Hijriani, Baiq Isti; Kurniawan, Edy
JSN : Jurnal Sains Natural Vol. 1 No. 4 (2023): November
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v1i4.416

Abstract

Bakteri endofit merupakan bakteri yang mampu menghasilkan senyawa-senyawa bioaktif yang sama dengan tanaman inangnya, salah satunya senyawa antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri bakteri endofit daun pepaya (Carica papaya L) terhadap Staphylococcus aureus, Untuk menentukan daya hambat atau zona hambat bakteri endofit terhadap Staphylococcus aureus. Jenis penelitian adalah eksperimental laboratorium. Pengujian menggunakan metode Difusi sumuran pada media MHA. Dilakukan 5 perlakuan yaitu pada Replikasi 1, 2, 3, Kontrol positif ciprofloxacin, kontrol negative aquadest. Hasil pengujian aktivitas antibakteri dilakukan pada isolat yang telah dimurnikan dengan metode difusi sumuran yang menunjukkan bahwa pada bakteri uji Staphylococcus aureus tidak memiliki aktivitas antibakteri.
Perbedaan Hasil Hematokrit Metode Mikro menggunakan Antikoagulan Alternatif Bawang Putih Volume 35, 40, dan 45 Mikroliter Hadiatun, Nurul; Hijriani, Baiq Isti
JSN : Jurnal Sains Natural Vol. 2 No. 3 (2024): Agustus
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v2i3.551

Abstract

Salah satu pemeriksaan hematologi yang digunakan sebagai penegak diagnosis, skrining DBD, anemia, dan polisitemia adalah pemeriksaan hematokrit. Antikoagulan yang biasa digunakan adalah EDTA. Bawang putih adalah salah satu tanaman yang dapat digunakan sebagai antikoagulan alternatif, karena mengandung senyawa ajoene yang berkontribusi dalam aksi antikoagulan, mengingat daerah terpencil susah untuk mendapatkan antikoagulan. Perbandingan volume antikoagulan alternatif bawang putih dengan darah harus tepat karena mempengaruhi nilai hematokrit. Tujuan dari penelitian ini adalah untuk menentukan apakah ada perbedaan dalam hasil hematokrit metode mikro dengan antikoagulan bawang putih volume 35 ?L, 40 ?L, dan 45 ?L. Desain penelitian adalah eksperimen dengan uji statistik menggunakan uji One Way Anova. Hasil penelitian menunjukan rerata nilai hematokrit pada antikoagulan alternatif bawang putih dengan volume 35 µL sebesar 41,67%, 40µL sebesar 40,78%, 45 µL sebesar 38,67% sedangkan rerata nilai hematokrit dengan antikoagulan EDTA sebesar 41,67%. Berdasarkan hasil penelitian tersebut menunjukkan bahwa tidak ada perbedaan yang signifikan volume antikoagulan alternatif bawang putih volume 35µL, 40µL dan 45µL terhadap hasil pemeriksaan hematokrit yang ditunjukan dari nilai p > 0,05 yaitu 0,125
GAMBARAN BAKTERI Eschericia coli PADA CINCAU HITAM DENGAN METODE MPN DI PASAR KOTA MALANG Hadiatun, Nurul; Anisa, Yufi Yuni; Hijriani, Baiq Isti
JSN : Jurnal Sains Natural Vol. 1 No. 2 (2023): May
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.16 KB) | DOI: 10.35746/jsn.v1i2.343

Abstract

Black cincau is a food that is consumed by many people. Black cincau is used as a mixture of drinks and facilitates the digestive system. Black cincau is widely traded in traditional markets, but the processing and presentation of blackcincau often ignores cleanliness or is unhygienic. Some are sold without special packaging and placed in open containers. Such conditions allow black cincau to be contaminated by dust and bacteria. Bacteria that often contaminate food are Coliform bacteria such as Escherichia coli. Some of the diseases caused by E. coli include; diarrhea, urinary tract infection, sepsis, and meningitis. The aim of this study was to describe the E. coli bacteria contaminating black cincau in the Malang city market. This type of research uses a descriptive method, namely identifying Escherichia coli bacteria by means of the MPN test. The results showed that there were two out of a total of five positive samples containing E.coli bacteria. The presence of E.coli bacteria in positive samples was indicated by the presence of metallic green colonies on EMBA media
Inhibitory potential of Japanese papaya (Cnidoscolus aconitifolius) leaf juice against the growth of Escherichia coli Hijriani, Baiq Isti; Solihin, Solihin; Kurniawan, Edy; Atfal, Bustanul
JSN : Jurnal Sains Natural Vol. 3 No. 2 (2025): Agustus
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v3i2.858

Abstract

Indonesia is a country with high biodiversity that have many plants, one of them is japanesse pepaya plant (Cnidoscolus aconitifolius). Japanese pepaya leaves used by people as a traditional medicine, this is because pepaya leaves contain compounds that can act as antibacterials. Escherichia coli is a normal flora that found in the human large intestine (colon). Escherichia coli can cause primary infections, one of the infections is diarrhea. The purpose of this study was to determine the ability of Japanese pepaya leaf juice (Cnidoscolus aconitifolius) to inhibit the growth of Escherichia coli bacteria and to determine the concentration of Japanese pepaya leaf extract that is effective in inhibiting the growth of Escherichia coli bacteria. This type of research is a laboratory experiment. The inhibition test used the Kirby Bauer agar diffusion method. The research variables were the concentration of Japanese pepaya leaf extract of 25%, 50%, 75%, and 100%, and the growth inhibition zone of Escherichia coli bacteria. The results of this study showed that no inhibition zone formed around the wells when Japanese pepaya extract was administered at various concentrations. The conclusion of this study is that Japanese pepaya (Cnidoscolus aconitifolius) leaf juice has no potential to inhibit the growth of Escherichia coli bacteria.
Edukasi Kesehatan dan Pemeriksaan Kesehatan Gratis dalam Upaya Meningkatkan Derajat Kesehatan Masyarakat Desa Banyumulek Lombok Barat Atfal, Bustanul; Hijriani, Baiq Isti; Hadiatun, Nurul; Ismatullah, Nurul Khatimah; Fardani, Roushandy Asri
Bakti Sekawan : Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2025): Juni
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/bakwan.v5i1.783

Abstract

Non-communicable degenerative diseases are chronic conditions that do not spread from one individual to another. The incidence of non-communicable diseases continues to increase, along with changes in people's lifestyles that tend to be unhealthy. In NTB Province, some of the main risk factors for non-communicable diseases include hypertension, obesity, and diabetes. The low level of public knowledge and awareness of the dangers of these diseases makes early detection services at health facilities less than optimal. In addition, many people are reluctant to have themselves examined if they have not felt any symptoms of illness. Therefore, risk factor management approaches and community-based prevention efforts are important steps to reduce the risk and mortality of non-communicable diseases. This activity aims to increase public awareness about the importance of routine health checks as part of early detection of non-communicable diseases to improve public health status. The activity was carried out through two methods, namely interactive counseling and direct health checks on participants. In general, the examination results showed a fairly good health condition of the participants with most of the results being within normal limits. However, there were seven participants with blood sugar levels exceeding 300 mg/dL. Activities like this are considered effective in raising public awareness of the importance of routine health checks as a preventive measure for non-communicable diseases.
Co-Authors Adriyan Suhada Agung Dwi Wahyu Widodo Aini aini aini aini Aini, Aini Ainutajriani Ajeng Dian Pertiwi Ajeng Dian Pertiwi, Ajeng Dian Al-Fariz, Rifqi Aminullah, Andi Muhammad Anisa, Yufi Yuni Bustanul Atfal Denih Agus Setia Permana Detty Agustin Riscal Dewi, Fitriani Kusuma Dhika Juliana Sukmana Dwi Monika Ningrum Edy Kurniawan, Edy Fardani, Roushandy Asri Faujiah, Putri Hardani, H Irawansyah Irawansyah Ismatullah, Nurul Khatimah Liah Kodariah ManikRetno Wahyunitisari Mentari, Ika Nurfajri Nurul Hadiatun Nurul Khatimah Ismatullah Pauzan RAI WIADNYA, IDA BAGUS Sakaroni, Rubiyatna Siska Zafrida Siti zaetun Siti Zaetun, Siti Solihin Solihin Sri Idawati Sri Sahbany Ulya, Tuhfatul ustiawaty, jumari Ustyawati, Jumari Widya sriwarsih Wirdullutfi, Wirdullutfi Wulan Ratia Ratulangi