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Diversity and the Composition of Fatty Acids of Lipolytic Bacteria Isolated from Soil and Aquatic Sediment in a Forest and on an Oil Palm Plantation Wijayanti, Marini; Meryandini, Anja; Wahyudi, Aris Tri; Yuhana, Munti
Makara Journal of Science Vol. 18, No. 3
Publisher : UI Scholars Hub

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Abstract

The specific bacteria in forests and on oil palm plantations are lipolytic bacteria. Their enzymes have been applied in the agro-aquaculture, food, detergent, pharmaceutical, dairy, and biodiesel-biokerosene industries. This study describes the diversity of cultivable lipolytic bacteria from soil and aquatic sediment in a forest and on an oil palm plantation and their fatty acid products. Soil samples used in this research were obtained from topsoil in a lowland forest and on an oil palm plantation and from sediments in fresh water near these sites. The forest is located in Bukit Duabelas National Park, and the oil palm plantation is near the forest in Sarolangun District, Jambi Province, Indonesia. Twenty-two isolates of lypolitic bacteria were selected from 32 isolates grown in lipolytic selective medium. The 22 consisted of 11 isolates from topsoil and 11 from aquatic sediment from the forest and plantation area. These isolates were identified by 16S rRNA-sequence data analysis. Taxonomically, they belonged to five genera: Burkholderia, Cupriavidus, Serratia, Acinetobacter, and Kurthia. The maximum likelihood tree showed that they are phylogenetically distributed in three clusters. They were clustered into three groups: the Burkholderia-Cupriavidus group, the Serratia-Acinetobacter group, and the Kurthia group. Their lipolytic enzymes formed various fatty acids after analysis by gas chromatography-flame ionization detector (GC-FID). Some isolates formed essential fatty acids, such as linoleic, linolenic, arachidonic, eicosapentanoic acid (EPA), and docosahexanoic acid (DHA).
Pemanfaatan Kapur Cangkang Keong Mas pada Air Rawa Media Pemeliharaan Ikan Lele dengan Model Budikdamber Dade Jubaedah; Marsi; Feni Haryani; Wijayanti, Marini
Jurnal Galung Tropika Vol 13 No 2 (2024)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v13i2.1201

Abstract

One of the currently developing urban farming models is budikdamber, which is a mutually beneficial combination of aquaculture and hydroponic systems with a bucket as a medium for cultivation. This research aims to find out the best dosage of golden snail shells lime for increasing the swamp water pH for rearing catfish (Clarias sp.) and kale using the budikdamber model. This research applied a completely randomized design with six treatments and three replications. The dosages of golden snail shells lime used as treatment consist of 0.00 mg L-1 equivalent to CaO (P0), 10 mg L-1 equivalent to CaO (P1), 20 mg L-1 equivalent to CaO (P2), 30 mg L-1 equivalent to CaO (P3), 40 mg L-1 equivalent to CaO (P4) and 50 mg L-1 equivalent to CaO (P5). The results of this study indicate that the best dose of golden snail shell lime is 20 mg L-1 equivalent to CaO (P2), which can optimize the swamp water pH from 4.80 to 6.78, survival rate 84 %, absolute weight growth of 18.44 g, absolute length growth of 6.15 cm and feed efficiency 132.49 %, and the total weight of the kale plant 11.33 g.
Eukaryote microbes potential for bioflocs in the swamp aquaculture Marini Wijayanti; Tanbiyaskur; Jubaedah, Dade; Saputra, Ade Bayu; Genti, Karta Sari; Agustina; Nabila Saraswati; Siti Yuliani; Hary Widjajanti
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.034 KB) | DOI: 10.19027/jai.19.1.19-29

Abstract

ABSTRACT Eucaryote microbes have an important role in forming bioflocs in the brackishwater aquaculture ponds. Bioflocs become potential live feed for milkfish or crustacea. This study aimed to identify the potential of eukaryotic microbes in the brackishwater aquaculture as the biofloc candidates potential development. This study was done through the water quality assessment and potential indigenous microbes isolation approach. Sampling was retrieved from the water subcomposite and sediment on each intertidal inlet and outlet. The water quality of brackishwater pond and intertidal swamp tended to lack of nutrient as containing inoptimal dissolved oxygen, salinity, pH, and ammonia content for the milkfish culture. Therefore, liming and fertilizing should be done to enrich the indigenous microbes. Eucaryote microbe isolated from the enrichment media was the aquatic fungi (multicellular and unicellular fungi) and microalgae (bacillariophyta, chlorophyta, and cyanophyta). Fungi and yeast formed a floculation with microalgae. This form will become a biofloc candidate as a live feed and water quality controller for the development of brackishwater aquaculture in the swamp area. Keywords: eucaryote microbes, biofloc, aquaculture, pond, brackish water swamp ABSTRAK Mikrob eukariot berperan penting dalam pembentukan bioflok di tambak budidaya pada lahan rawa payau. Bioflok menjadi pakan alami untuk budidaya ikan bandeng atau udang. Tujuan riset ini adalah mengidentifikasi mikrob eukariot rawa payau potensial untuk pengembangan kandidat bioflok di akuakultur rawa payau. Riset ini dilakukan dengan pendekatan kualitas air serta isolasi mikrob indigenous tambak dan rawa pasang surut untuk mendapatkan isolat mikrob potensial sebagai kandidat pembentuk bioflok. Sampling dilakukan secara subkomposit pada air dan sedimen di setiap inlet dan outlet pasang surut. Kualitas air tambak dan rawa pasang surut menunjukkan kondisi oksigen terlarut, salinitas, pH, dan amonia berada di luar batas optimum budidaya ikan bandeng, sehingga perlu dilakukan pengapuran dan pemupukan untuk memperbanyak mikrob indigenous-nya. Isolasi mikrob eukariot dari media pengayaan diperoleh jenis fungi akuatik yang multiseluler dan uniseluler, sedangkan mikroalga yang diperoleh yaitu dari golongan Bacillariophyta, Chlorophyta, dan Cyanophyta. Fungi yang dapat membentuk hifa dapat merangkai mikroalga dan khamir untuk membentuk flok. Susunan ini diharapkan dapat menjadi bahan bioflok yang berguna sebagai pengendali kualitas air sekaligus pakan alami bagi pengembangan budidaya di lahan rawa payau. Kata kunci: mikrob eukariot, bioflok, budidaya, tambak, rawa payau
Bacterial Communities in Aquatic Sediment from Bukit Duabelas Raintforest and Oil Palm Plantation at Sumatra Indonesia Wijayanti, Marini; Wahyudi, Aris Tri; Yuhana, Munti; Engelhaupt, Martin; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1289.48 KB) | DOI: 10.4308/hjb.25.2.85

Abstract

The diversity of bacterial communities in aquatic sediment of rainforest and oil palm plantation at Sumatra was studied using pyrosequencing of 16S rRNA gene and common biodiversity indices. Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas. Ecological approach used soil pH, total Carbon (TC), total Nitrogen (TN), available Phosphorus (AP) measurement and bacterial diversity with Shanon and Simpson indices, and bacterial richness with Chao1-ACE indices and OTUs. Bacterial diversity and richness in aquatic sediment of forest area was higher than that ones in oil palm plantation area, although their pH, TC, and TN in both areas were not different significantly. The majority of sequences related to Proteobacteria (34.85%), Acidobacteria (32.67%), Nitrospirae (6.86%), Chloroflexi (4.31%), and Actinobacteria (4.02%) were from forest; whereas those related to Acidobacteria (46.10%), Proteobacteria (25.86%), Nitrospirae (9.20%), Chloroflexi (4.99%), and Actinobacteria (2.34%) invented from oil palm plantation. The genera of Alphaproteobacteria and Betaproteobacteria dominated in genera phylotype of bacterial 16S rRNA phylogenetic revealed both aquatic sediment of forest and oil palm plantation. The most genera in the phylogenetic tree from aquatic sediment of both areas was Burkholderia. The bacterial community shift in aquatic sediment of forest transformation indicated higher bacterial diversity index, richness index, some of phyla and genera in aquatic sediment from forest than from oil palm plantation.
Different effects of swamp probiotics application frequency as a biofloc-forming agent on the production of catfish (Clarias gariepinus) Amin, Mohamad; Mukti, Retno Cahya; Taqwa, Ferdinand Hukama; Andini, Andini; Marsi, Marsi; Priyanto, Langgeng; Wijayanti, Marini
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.34280

Abstract

Catfish (Clarias gariepinus) that are reared with probiotics as biofloc-forming agent is thought to increase the fish production. Applying swamp probiotics to the water media has never been studied to ensure the flocks' availability in the rearing media. This study aimed to determine the appropriate frequency of probiotics application collected from swamps for biofloc formation to improve the catfish production. This study used a completely randomized design with two treatments and three replications. The treatments were composed of different application frequency of swamp probiotics: (P1) once in 42 days of rearing and (P2) twice in 42 days of rearing. Data on flock volume, total bacterial colonies, absolute growth rate, feed efficiency, survival rate, and water quality were analyzed by T-test with a 95% confidence level. Meanwhile, the flock composition data were analyzed descriptively. The results showed that P2 obtained the best treatment with a floc volume of 68.33 10.41 mL/L, absolute length growth of 8.18 1.03 cm, absolute weight growth of 19.30 3.12 g, feed efficiency of 135.24 7.98%, survival rate of 89.33 6.21%, biomass production of 24639.50 1344.51 g, temperature of 28.85-29.59C, pH of 7.27-7.42, dissolved oxygen (DO) of 3.91-5.72 mg/L, ammonia of 0.45-1.15 mg/L, and total dissolved solids (TDS) of 717.33-885.50 mg/L. Therefore, swamp probiotics should be applied to catfish culture media twice for 42 days of rearing or once every 21 days.Keywords:BioflocCatfishProbiotics from swamp
Impact of Probiotic Swamp Bacteria on the Prevention of Aeromonas hydrophila Infection in Striped Snakehead (Channa striata) Wijayanti, Marini; Sasanti, Ade Dwi; Jubaedah, Dade; Citra, Nyayu
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6631

Abstract

Probiotics are microbes that can modify the bacterial structure in fish digestive systems. Probiotics are used as feed additives and can improve the host's health. As a result, it can help to prevent Aeromonas infection. This study aims to determine the effectiveness of bacteria from the swamp (Bacillus sp. and Streptomyces sp.) on feed in preventing Aeromonas hydrophila infection in striped snakeheads. The study used P0 (control), P1 (Bacillus sp.), P2 (Streptomyces sp.), P3 (Bacillus sp. and Streptomyces sp. combination), each bacterium 10 ml kg-1 feed. Total of bacteria, prevalence, fish growth, survival rate, and water quality are among the variables assessed. The fish survival, fish growth, and feed efficiency in Channa treated with a combination of Bacillus and Streptomyces (P3) were significantly highest among other, resulting of best prevalence 2.38%.
Different effects of swamp probiotics application frequency as a biofloc-forming agent on the production of catfish (Clarias gariepinus) Amin, Mohamad; Mukti, Retno Cahya; Taqwa, Ferdinand Hukama; Andini, Andini; Marsi, Marsi; Priyanto, Langgeng; Wijayanti, Marini
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.34280

Abstract

Catfish (Clarias gariepinus) that are reared with probiotics as biofloc-forming agent is thought to increase the fish production. Applying swamp probiotics to the water media has never been studied to ensure the flocks' availability in the rearing media. This study aimed to determine the appropriate frequency of probiotics application collected from swamps for biofloc formation to improve the catfish production. This study used a completely randomized design with two treatments and three replications. The treatments were composed of different application frequency of swamp probiotics: (P1) once in 42 days of rearing and (P2) twice in 42 days of rearing. Data on flock volume, total bacterial colonies, absolute growth rate, feed efficiency, survival rate, and water quality were analyzed by T-test with a 95% confidence level. Meanwhile, the flock composition data were analyzed descriptively. The results showed that P2 obtained the best treatment with a floc volume of 68.33 10.41 mL/L, absolute length growth of 8.18 1.03 cm, absolute weight growth of 19.30 3.12 g, feed efficiency of 135.24 7.98%, survival rate of 89.33 6.21%, biomass production of 24639.50 1344.51 g, temperature of 28.85-29.59C, pH of 7.27-7.42, dissolved oxygen (DO) of 3.91-5.72 mg/L, ammonia of 0.45-1.15 mg/L, and total dissolved solids (TDS) of 717.33-885.50 mg/L. Therefore, swamp probiotics should be applied to catfish culture media twice for 42 days of rearing or once every 21 days.Keywords:BioflocCatfishProbiotics from swamp
Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum . TRI-PANJI; . SUHARYANTO; Marini WIJAYANTI
Menara Perkebunan Vol. 77 No. 1: 77 (1), 2009
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v77i1.113

Abstract

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
PENDAMPINGAN TEKNOLOGI PEMBENIHAN IKAN LELE MELALUI SISTEM TERKONTROL DALAM PENINGKATAN PRODUKSI BERKELANJUTAN DI DESA TALANG BALAI BARU I Taqwa, Ferdinand Hukama; Syaifudin, M; Fitrani, Mirna; Jubaedah, Dade; Wijayanti, Marini; Amin, Mohamad; Muslim, Muslim; Yulisman, Yulisman; Tanbiyaskur, Tanbiyaskur; Yonarta, Danang; Riswandi, Agung; Afriansyah, Azmi
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2298

Abstract

Desa Talang Balai Baru I adalah salah satu desa yang berada di Kecamatan Tanjung Raja, Kabupaten Ogan Ilir, Sumatera Selatan. Desa Talang Balai Baru I dikelilingi oleh sungai kelekar dan rawa yang kaya akan flora dan fauna. Desa Talang Balai Baru I Kecamatan Tanjung Raja, Kabupaten Ogan Ilir, Sumatera Selatan merupakan salah satu dari 50 desa ekowisata terbaik di Indonesia. Permasalahan yang dialami oleh pembudidaya  ikan termasuk yang dihadapi oleh kelompok pembudidaya ikan di Desa Talang Balai Baru I saat  ini belum mampu memproduksi benih ikan Lele secara mandiri dan kontinue sehingga ketersediaan benih untuk usaha pembesaran budidaya ikan lele masih mengandalkan dari hasil tangkapan alam. Untuk mengatasi masalah yang timbul dan untuk meningkatkan produksi khususnya pembudidaya ikan lele maka perlu ditingkatkan usaha budidaya yang lebih intensif. Salah satu upaya yang dapat dilakukan adalah dengan menambahkan atau menyuntikkan hormon ovaprim ke dalam tubuh ikan yang sudah matang gonad untuk meempercepat proses pemijahan sehingga dapat dihasilkan benih ikan lele yang baik dimana jumlah, mutu dan waktu penyediaannya dapat diatur sesuai dengan yang diinginkan. Tujuan pengabdian masyarakat ini untuk meningkatkan kemampuan teknis pembudidaya ikan dengan transfer teknologi pembenihan secara terkontrol ikan lele dan meningkatkan produksi benih ikan lele secara berkelanjutan. Hasil produksi mitra sebelum adanya kegiatan PKM ini hanya mampu menangkap dari alam, sekarang sudah bisa melakukan pemijahan secara mandiri.
APLIKASI KAPUR CANGKANG KERANG DARAH (Anadara granosa) DENGAN DOSIS BERBEDA PADA TANAH GAMBUT UNTUK BUDIDAYA IKAN PATIN (Pangasius sp.) Marsi, Marsi; Jubaedah, Dade; Wijayanti, Marini; Maswala, Depi
JARI : Jurnal Akuakultur Rawa Indonesia Vol. 13 No. 1 (2025): JARI : JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v13i1.35

Abstract

The application of lime is attempt to increase the pH of the soil and pond water for catfish culture in swamps which generally have low pH. Liming is not only affected by soil and water pH, but also the content of soil organic carbon. The purpose of this study was to determine the dose of blood cockle shell lime on the bottom soil of the pond in increasing the pH of the water culture medium as well as the survival and growth of catfish. This study used a completely randomized design (CRD) with 6 treatments and 3 replications.The treatments given were differences in the dosage of cockle shells lime, consisting of 6,000 kg ha-1 equivalent to CaO (P1), 8,000 kg ha-1 equivalent to CaO (P2), 10,000 kg ha-1 equivalent to CaO (P3), 12,000 kg ha-1 equivalent to CaO (P4), 14,000 kg ha1 equivalent to CaO (P5), and 16,000 kg ha-1 equivalent to CaO (P6). The results of this study indicate that P6 with a dose of 16, 000 kg ha-1 equivalent to CaO increased the initial soil pH from 3.2 to 7.8. As for water pH, the best dose is P3 treatment with a dose of 10,000 kg ha-1 equivalent to CaO which increases the initial pH from 3.40 to 6.97 and produces 100% survival of catfish, an absolute length of growth 5.52 cm and absolute of weight growth 11.15 g with feed efficiency of 97.14%.
Co-Authors Ade Bayu Saputra Ade Dwi Sasanti Ade Dwi Sasanti Ade Dwi Sasanti Afriansyah, Azmi Agustina Aldila Din Pangawikan Andini, Andini Anja Meryandini Anjastari, Devi Annisa Siregar Arafsanajani Arif Aris Tri Wahyudi Aritonang, Lilis Pritamasari Chandra Irsan Choirul Amar Simbolon Citra, Nyayu Dade - Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Danang Yonarta Danang Yonarta Dedi . Apriadi Devi Anjastari Dwi Rizki Septiana Eka Febri Fitriana Engelhaupt, Martin Erwin Nofyan Feni Haryani Ferdinand Hukama Taqwa Ferdinand Hukama Taqwa Fitriana, Eka Febri Genti, Karta Sari Gracia Eirene Girsang Hary Widjajanti Hendro Widyantoro Herdia Agustina Hijral Hamdani Husnul Khotimah Ida Handayani Ismalia, Nova Junedi Tinambunan Kezia Agustine Riyadhi Kukuh Jaya Langgeng Priyanto, Langgeng M Aiman Ziyad M Syaifudin Madyasta Anggana Rarassari Madyasta Anggana Rarassari Madyasta Anggana Rarassari Madyasta Anggana Rarassari Maemonah, Maemonah Marsi Marsi - - Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Maswala, Depi Maulidya, Ainayyah Mirna Fitrani Mirna Fitrani Mirna Fitrani Mochamad Syaifudin, Mochamad Mohamad Amin Muhammad Fadhil Akbar Mulyani, Yenni Sri MUNTI YUHANA Muslim Muslim Muslim Muslim Nabila Saraswati Novi Wulandari Mustika NUNI GOFAR Nurwijayanti Nyayu Nurul Husna Olivia Riana Sari Rarassari, Madyasta Anggana Retno Cahya Mukti Ria octaviani Riswandi, Agung Saputra, Ade Bayu Sefti Heza Dwinanti Sefti Heza Dwinanti Sefti Heza Dwinanti Sefti Heza Dwinanti Septika Putri Anggraini Septimesy, Annisa Septiyani, Reni Siti Yuliani Sofiatul Rahmani Soikar Hariadi Suharyanto Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur, Tanbiyaskur Tri Panji Valencia Aff Neka Wati, Rizka Yonarta, Danang Yonarta, Danang Yudha Galih Wibawa YULISMAN Yulisman Yulisman Yulisman Yulisman