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All Journal HAYATI Journal of Biosciences Farmaka Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan The Indonesian Biomedical Journal Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) JMM (Jurnal Masyarakat Mandiri) Jurnal Kreativitas PKM FITOFARMAKA: Jurnal Ilmiah Farmasi Current Research on Biosciences and Biotechnology Indonesian Journal of Biological Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Kesehatan Sciences of Pharmacy Public Health Risk Assesment Journal Medical Sains : Jurnal Ilmiah Kefarmasian
Melisa Intan Barliana
Fakultas Farmasi Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Journal : HAYATI Journal of Biosciences

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Anticancer Activities and Metabolite Profiling of UHPLC-HRMS Method from Chrysanthemum x morifolium (Ramat.) Hemsl Leaves Maesaroh, Imas; Barliana, Melisa Intan; Abdulah, Rizky; Muhaimin, Muhaimin
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.167-180

Abstract

The Chrysanthemum morifolium Ramat, traditionally used for cancer treatment, including breast cancer, possesses anticancer properties. The aim of this study is metabolite profiling using ultra-high-performance liquid chromatography in conjunction with the high-resolution mass spectrometry (UHPLC-HRMS) technique and its correlation with the cytotoxic activity of the extract and ethyl acetate fraction of Chrysanthemum x morifolium (Ramat.) Hemsl leaves on cancer cells. The ethyl acetate fraction from the hydrolyzed ethanol extract of (Chrysanthemum x morifolium (Ramat.) Hemsl) leaves has anticancer activity against the MCF-7 breast cancer cells. Metabolite profiling was used to understand the presence of metabolites that have anticancer activity. UHPLC-HRMS was used to profile their metabolites. Compound Discoverer 3.3 software finished data processing and metabolite annotation. Anticancer activity was performed using the 2-[2-methoxy-4-nitrophenyl]-3[4-nitrophenyl]-5[2,4-disulfophenyl]-2H-tetrazolium (WST-8) assay. As many as 57 secondary metabolites were identified by UHPLC-HRMS analysis. Secondary metabolites that have the potential as anti-breast cancer are glycitein, diosmetin, kaempferol, esculetin, scopoletin, dihydroartemisinin, and Chrysin, with successive percentages of 31.39%, 19.91%, 5.61%, 2.63%, 0.82%, 0.14%, and 0.05%. Ethyl acetate fraction showed stronger cytotoxic activity than ethanol extract against MCF-7 cells with IC50 values of 66.31 ppm at 24 hours incubation and 40.35 ppm at 48 hours. Further research can be conducted on the isolation of flavonoids from the ethyl acetate fraction, as well as the analysis of cell cycle apoptosis stimulation and gene expression mechanisms.
Co-Authors Abdurahman Ridho Afifah, Nadya Nurul AGATHA VIKA PURWANINGTYAS Ahmad Faried Ahmad Muhtadi Ajeng Diantini, Ajeng Aliya Nur Hasanah Among, Sayyid Andi Wijaya Andi Wijaya ANDI WIJAYA Angliana Chouw, Angliana Anna Meiliana Anna Meiliana ARNANDA, QUINZHEILLA PUTRI Balqist, Syara Nur Fitri Bonor, Toga Chandra Agung Purnama Cissy Bana Kartasasmita Cynthia Retna Sartika, Cynthia Retna Dedi Suyatno Dewi Muliaty Didi Susanto Driyanti Rahayu Edhyana Sahiratmadja Effendi, M. Fariz ELLENA MAGGYVIN Erizal Sugiono Eva Feriadi Fajar, Desi Reski Febio Gutama Febriyanti, Maya Haifa, Rima HISBAN HAMID ARIFKI Ida Paulina Sormin Imas Maesaroh Indradi, Raden Bayu Intania, Ruri Irma Melyani Puspitasari Irma Rahayu Latarissa Irmansyah Irmansyah Ivan Pradipta Ivan Surya Pradipta Karina Erlianti Kennardi, Gabriel Bagus Keri Lestari Khaira, Syifa Komang Suma Triyasa Laniyati Hamijoyo, Laniyati LILY CYNTIA FAUZI Luki Yogaswara Yusuf Made Putra Semadhi Maisyarah, Intan Timur Maya Febriyanti Miftakh Nur Rahman Miswar Fattah Moses Prasetio Muhaimin Muhaimin Muhammad Fauzi Munir Alinu Mulki Mustikawati, Bunga Nadiya Nurul Afifah Nuning Nurcayani Peratiwi, Shafira Galuh Permatasari, Lany Indah Pratama, Muhammad Syawal Prayudi Santoso Putri Maharani Putri, Nazwa Septiriana Qolbina, Shofura Marsa Raden Maya Febriyanti Rendrayani, Farida Rimadani Pratiwi Rizki Rahmadi Pratama Rizky Abdulah Sahila, Elma N. M. R. Sartika Aprianti Shelly Iskandar Soemarie, Yulistia Budianti Syahrina, Nadira Alvi Tahara, Nabila Theresia Ratnadevi TIANA MILANDA Tina Rostinawati Widya Naftalia Wijasa Yudisia Ausi Yulistia Budianti Soemarie Yulistia Budianti Soemarie Yuni Elsa Hadisaputri Zakiyah, Neily Zelika Mega Ramadhania