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The Effect of Black Pepper (Piper nigrum L.) Extract on Morphology and number of Mice (Mus musculus L.) Spermatozoa Induced by Progestin Hormone Indriyani; Sutyarso; Busman, Hendri; Nurcahyani, Nuning; Setyaningrum, Endah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.384

Abstract

The progestin hormone is used as male hormonal contraception. Giving progestin to normal men can  suppress libido and testicular function effectively, and reduce testosterone levels so that it can interferer with the spermatogenesis process. This study is about the effectiveness of black pepper (Piper nigrum L.) extract on morphology and number of spermatozoa in mice (Mus musculus L.)  induced by progestin. This study used a completely randomized design (CRD) which has divided into four treatment groups, each group contained 5 mice and treated according to the experimental design. Group P0 as a control (given H2O), P1 (induced by 1,25 mg progestin), P2 (induced by 1,25 mg progestin and 3.33mg/Kg BW ethanol extract of black pepper), and P3 (induced by 1,25 mg progestin and 3.33mg/Kg BW distilled water extract of black pepper). The Progestin hormone is given twice (week 1 and week 2). Black pepper extract was given daily for 35 days. Data were analyzed by one-way ANOVA and continued with the LSD test with a level of 5%. The result showed that giving of ethanol extract and distilled water extract from black pepper could improve the normal morphology and number of spermatozoa in mice after induced by progestin hormone so that fertility in men increases.
Uji Efektivitas Ekstrak Etanol Daun Mahoni (Swietenia mahagoni L.) Dalam Sediaan Losion Sebagai Repelan Terhadap Nyamuk Aedes aegypti Rosari, Anastasia Santaulina Putri; Setyaningrum, Endah; Pratami, Gina Dania; Widiastuti, Endang Linirin
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56344

Abstract

Preventive efforts made to avoid mosquito bites generally use repellants containing synthetic chemicals in the form of N, N-diethyl-meta-toluamide (DEET) and if used for a long period of time can cause damage to the human body. It is known that mahogany plants (Swietenia mahagoni L.) contain secondary metabolite compounds that have the potential as insect repellants. This study aims to determine the effectiveness of ethanol extract of mahogany leaves (S. mahagoni L.) in lotion as a mosquito repellent for Ae. aegypti, Effective Concentration 50% (EC50), physical properties and irritating effects of mahogany leaf ethanol extract lotion. This research was conducted in January 2024 - March 2024 at the Botany Laboratory and Zoology Laboratory, FMIPA, Unila. The study used a completely randomized design (CRD) with 4 treatments and 6 repetitions consisting of 4 levels of test concentrations, namely 0%, 5%, 10%, and 15%. The results of this study showed that the lotion of ethanol extract of mahogany leaves at a concentration of 15% had a protective power as a repellant of 87.63%, the EC50 value of the extract lotion at a concentration of 9,60%. The lotion has good physical properties and does not cause irritation on the skin. In conclusion, ethanol extract of mahogany leaves has effectiveness as a repellent for Aedes aegypti mosquitoes.
Larvicide Effects of Serratia marcescens strain MBC1 Extract on Instar III Larvae of Aedes aegyti Lestari, Mutia Dinda; Nukmal, Nismah; Setyaningrum, Endah; Farisi, Salman; Arifiyanto, Achmad
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.219

Abstract

Bakteri merupakan kandidat yang berpotensi sebagai larvasida Aedes aegypti. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak Serratia marcecsens strain MBC1 terhadap kematian larva instar III Aedes aegypti. Penelitian ini merupakan penelitian eksperimental dengan dua macam bentuk pengujian, yaitu uji larvasida untuk menentukan mortalitas dan uji lanjut untuk melihat perkembangan larva setelah perlakuan larvasida. Larva instar III diberi perlakuan dengan konsentrasi 125, 250, 500, dan 1.000 ppm. Uji larvasida diamati selama 24-72 jam. Uji lanjut larva instar III  diberi perlakuan dengan konsentrasi 500 dan 1.000 ppm serta diamati sampai semua larva mati. Kontrol positif menggunakan Abate® 1% dan kontrol negatif menggunakan air sumur. Hasil analisis probit didapatkan bahwa nilai LC50 dan LC90 ekstrak S. marcescens adalah 66.426,02 dan 749.001,41ppm. Larva instar III mati pada 18 hari setelah perlakuan ekstrak S. marcescens strain MBC1. Hasil dari penelitian ini menunjukkan bahwa larvasida ekstrak S. marcescens mempengaruhi lamanya siklus hidup dan mati sebelum mencapai stadium dewasa.     Bacteria have potencial as larvicide of Aedes aegypti. The purpose of this study is to determine the effect of Serratia marcescens strain MBC1 against larval third instars of Aedes aegypti. This research was an experimental study with two kinds of testing. The larvicide test to determine the mortality of the larval and the continuous test to observe the development of the larval after treatment with the larvacide. The third instars of mosquito larval were given bacterial extract in four concentrations (125, 250, 500, and 1000 ppm), and observed mortality during 72 hours. The continuous larvicide test was observed at two concentrations (500 and 1,000 ppm). The observation is completed when all of the larvae died. The positive control used Abate® 1% and the negative control used natural water. The treatment was repeated 4 times. The results show that LC50 and LC90 of S. marcescens extract are 66,426.02 dan 749,001.41 ppm.  All of the larvae in the continuous test die after 18 days have been givenan extract of S. marcescens strain MBC1. The experiment shows that the larvicide of extract of S. marcescens influences the length of the life cycle and larvae of Ae. aegypti die before reaching the adult.    
Effect of Pyriproxyfen, a Malaria Vector Larvicide, on the Growth and Mortality of Vannamei Shrimp Putri, Sriana; Setyaningrum, Endah; Farisi, Salman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.258

Abstract

Anopheles mosquito shared a breeding site with the vannamei shrimp (Litopenaeus vannamei). Anopheles mosquito larvae commonly live in both stagnant fresh and brackish water. The potential breeding habitat of Anopheles mosquito larvae may be rice fields, pools or ponds, swamps, ditches, and slow-current streams. One of the effective strategies to control malaria is to apply chemical insecticides. Pyriproxifen is a larvicide that contains juvenile hormone-like compounds. Juvenile hormone-like compounds can affect mosquito morphogenesis, characterized by the failure of the larvae to develop into pupae. With a completely randomized design, the shrimp fry was divided into 4 groups,  6 repetitions, 20 individuals each. Shrimp fry in each experimental unit was placed and maintained in plastic jars containing 2.5 L of brackish water. The results showed that the insect growth regulator (IGR) with the active ingredient pyriproxyfen at various concentrations had no significant effect on the growth, mortality, and survival of vannamei shrimp fry.  It can be concluded therefore that pyriproxifen larvicide was safe to be used for controlling malaria mosquito vectors in hatcheries and breeding ponds of vannamei shrimp.
Potensi Bioinsektisida Ekstrak Air Dan Metanol Gracilaria sp. Terhadap Aedes aegypti Dengan Metode Spray Pertiwi, Widya Ratna; Pratami, Gina Dania; Setyaningrum, Endah; Nurcahyani, Nuning
Jurnal Medika Malahayati Vol 9, No 2 (2025): Volume 9 Nomor 2
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v9i2.19122

Abstract

Penggunaan insektisida kimiawi digunakan dalam upaya pengendalian vektor DBD. Namun, penggunaannya dapat digantikan dengan rumput laut Gracilaria sp. karena mampu bersifat toksik terhadap serangga. Tujuan penelitian ini adalah mengetahui efektivitas ekstrak air dan metanol Gracilaria sp. terhadap mortalitas nyamuk Ae. aegypti serta mengetahui nilai LC50 ekstrak air dan metanol Gracilaria sp. yang efektif dijadikan sebagai bioinsektisida. Penelitian ini dilakukan secara eksperimental menggunakan RAK dengan 7 perlakuan yang terdiri dari 3 konsentrasi (10%, 15%, dan 20%) ekstrak air dan metanol Gracilaria sp., dan transfluthrin, serta dilakukan 3x pengulangan. Analisis One Way ANOVA menunjukkan perbedaan signifikan terhadap jumlah kematian nyamuk dengan nilai p-value= 0,00 (P ≤ 0,05). Uji LSD menunjukkan perbedaan signifikan antara ekstrak air Gracilaria sp. konsentrasi 20% terhadap ekstrak air Gracilaria sp. konsentrasi 10%, 15%, ekstrak metanol Gracilaria sp. konsentrasi 10%, 15%, dan 20%, serta transfluthrin. Nilai LC50 ekstrak air Gracilaria sp. diperoleh sebesar 33,011%, sedangkan ekstrak metanol Gracilaria sp. sebesar 22,961% dan dapat disimpulkan ekstrak air dan metanol Gracilaria sp. memiliki potensi sebagai bioinsektisida karena mampu membunuh nyamuk Ae. aegypti sebesar 13,3%-40%.
Ecological Study of Mosquito Larvae Breeding Site Anopheles Malaria Vector in Abandoned Ponds Hanura Village Setyaningrum, Endah; Qoyima, Allafia; Priyambodo, Priyambodo; Pratami, Gina Dania
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.20802

Abstract

Malaria is an infectious disease that is common in tropical and sub-tropical climates, making malaria endemic areas. One of the villages in Teluk Pandan District, Pesawaran Regency, Hanura Village is close to an abandoned pond which is a breeding place for malaria vectors which is the cause of malaria cases in Pesawaran. The aim of this research is to determine the relationship between physical and chemical factors and the density of Anopheles larvae and to identify types of aquatic plants and aquatic animals and their influence on the density of Anopheles mosquito larvae. in Hanura Village, Teluk Pandan District. The method used in this research is a survey method and observation of several ecological factors in the breeding places of malaria mosquito vectors. The measurement results showed that physical and chemical factors in DO had a positive correlation with larval density. Biological factors found include chicken stomach moss (Enteromorpha intestinalis), sea turtles (Limnoporus canaliculatus), back swimmers (Notonecta undulata), snakehead fish (Channa striata), water beetles (Tropisternus sp.), and tadpoles (Anura). The higher the population of aquatic plants, the higher the density of mosquito larvae. Meanwhile, the higher the population of aquatic animals, the lower the density of mosquito larvae.
Uji Bioaktivitas Ekstrak n-Heksana Rumput Laut Gracilaria sp. Sebagai Antimalaria Terhadap Parasit Plasmodium falciparum Secara In vitro Yuandarru, Rhifana; Setyaningrum, Endah; Chrisnawati, Lili; Pratami, Gina Dania
Jurnal Penelitian Sains Vol 27, No 2 (2025)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v27i2.1170

Abstract

Tantangan utama dalam mengendalikan malaria yakni tingginya resistensi parasit terhadap obat antimalaria. Pendekatan yang dapat digunakan untuk menyelesaikan permasalahan tersebut yakni dengan mencari dan mengembangkan bahan alam potensial, seperti rumput laut Gracilaria sp. Penelitian ini bertujuan untuk mengidentifikasi kandungan senyawa fitokimia dan bioaktivitas antimalaria Gracilaria sp. dengan menggunakan pelarut n-Heksana. Penelitian ini dilaksanakan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL) untuk mengeksplorasi efek ekstrak n-Heksana dari Gracilaria sp. dengan berbagai konsentrasi terhadap parasit Plasmodium falciparum strain 3D7. Data persentase parasitemia, pertumbuhan, dan penghambatan parasit dianalisis secara statistik menggunakan ANOVA, serta analisis probit nilai IC50 menggunakan software SPSS berdasarkan data rata-rata persentase penghambatan. Hasil pengujian fitokimia memperlihatkan bahwasanya ekstrak n-Heksana mengandung senyawa metabolit sekunder Alkoloid, Flavonoid, Steroid, Terpenoid, Saponin, dan Tanin. Hasil uji bioaktivitas antimalaria menunjukan adanya penurunan data persentase parasitemia dan pertumbuhan, serta peningkatan data persentase penghambatan seiring dengan meningkatnya konsentrasi ekstrak. Berdasarkan data rata-rata persentase penghambatan, didapatkan nilai IC50 sebesar 181,61µg/ml yang mengindikasikan bahwa ekstrak memiliki potensi sebagai antimalaria tetapi tidak memenuhi kriteria sebagai antimalaria yang aktif.
Potensi Daun Kemangi (Ocimum sanctum) sebagai Insektisida Mat Elektrik untuk Pengendalian Vektor Demam Berdarah: Linda Septiani, Intan Kusumaningtyas, Terza Aflika Happy, Wiwi Febriani, Primasari Pertiwi, Endah Setyaningrum Septiani, Linda; Kusumaningtyas, Intan; Happy, Terza Aflika; Febriani, Wiwi; Pertiwi, Primasari; Setyaningrum, Endah
Jurnal Kedokteran Universitas Lampung Vol. 9 No. 1 (2025): JK UNILA
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jkunila.v9i1.pp112-116

Abstract

Demam Berdarah Dengue (DBD) masih menjadi masalah kesehatan masyarakat gobal dan khususnya di Indonesia. DBD ditularkan oleh vektor nyamuk Aedes aegypti sebagai vektornya. Penggunaan insektisida sintetis dalam jangka panjang dapat menimbulkan resistensi dan dampak lingkungan, sehingga diperlukan alternatif berbasis bahan alami. Daun kemangi (Ocimum sanctum) diketahui mengandung senyawa aktif seperti eugenol yang memiliki potensi sebagai insektisida alami. Penelitian ini bertujuan untuk mengkaji potensi ekstrak daun kemangi dari berbagai literatur sebagai bahan aktif dalam formulasi mat elektrik, yaitu pad pemanas penguap senyawa volatil yang bekerja sebagai pembunuh atau pengusir nyamuk. Berdasarkan kajian literatur dari berbagai studi eksperimental, penggunaan ekstrak kemangi dalam electric mat menunjukkan efektivitas yang tinggi dalam menurunkan populasi nyamuk Aedes aegypti, dengan tingkat mortalitas hingga 100% pada konsentrasi tertentu. Selain itu, metode aplikasi melalui mat elektrik terbukti mampu mengoptimalkan penguapan senyawa aktif, meningkatkan daya bunuh dan daya tolak terhadap nyamuk. Hasil ini menunjukkan bahwa daun kemangi berpotensi dikembangkan sebagai insektisida alami yang ramah lingkungan untuk pengendalian vektor DBD secara berkelanjutan. Kata Kunci: Demam Berdarah Dengue, daun kemangi, Insektisida, Mat Elektrik, Nyamuk Aedes aegypti
Diversity of Termite Species Based on Building Age in Balik Bukit District, West Lampung – Lampung Hasan, Moh. Dwi Kurniawan; Tugiyono, Tugiyono; Setyaningrum, Endah; Indarto, Indarto
Biosfer: Jurnal Tadris Biologi Vol 13 No 2 (2022): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v13i2.14175

Abstract

Termites in nature operate as cellulose decomposers, giving vital ecosystem services to nature, but they may also inflict significant damage to building structures. The durability of the wood construction of the building decreases with age, and the possibility of termite infestation increases. This study is a descriptive-analytic study that uses quantitative research methodologies and a cross-sectional research design. The study was carried out between May and July 2022 in Balik Bukit District, West Lampung Regency - Lampung. Building data gathering techniques were used by delivering questionnaires to 100 building owners utilizing accidental sampling techniques. Termites were discovered at the age of the building of more than 26 years, with the finding of four types of termites, namely Macrotermes gilvus (Hagen), Bulbittermes constrictiformis (Holmgren), Schedorhinortermes mediobcorus (Holmgren), and Coptotermes gestroi (Wasmann).ABSTRAK: . Rayap di alam bertugas sebagai pengurai bahan selulosa, menyediakan jasa ekosistem yang berharga bagi alam, namun mereka juga dapat menyebabkan sejumlah besar kerusakan pada struktur bangunan. Semakin tua umur bangunan maka keawetan konstruksi kayu yang ada di dalamnya cenderung menurun dan potensi serangan rayap akan semakin meningkat. Penelitian ini merupakan penelitian analisis deskriptif menggunakan metode penelitian kuantitatif dengan desain penelitian cross sectional. Penelitian dilakukan pada bulan Mei – Juli 2022 di Kecamatan Balik Bukit, Kabupaten Lampung Barat – Lampung. Teknik pengumpulan data bangunan menggunakan teknik accidental sampling dengan menyebarkan kuesioner pada 100 bangunan. Titik ditemukan rayap adalah pada umur bangunan >26 tahun dengan ditemukannya empat jenis rayap yaitu Macrotermes gilvus (Hagen), Bulbitermes constrictiformis (Holmgren), Schedorhinortermes mediobcorus (Holmgren), Coptotermes gestroi (Wasmann)
Spatial Analysis of Breeding Places of Anopheles sp. Mosquitoes as Potential Vectors of Malaria Infection in Pesawaran District, Lampung, Indonesia Athma, Dwi Aji; Suwandi, Jhons Fatriayadi; Mayaguezz, Henky; Setyaningrum, Endah; SRW, Dyah Wulan; Darmawan, Arief
Ruwa Jurai: Jurnal Kesehatan Lingkungan Vol. 19 No. 1 (2025)
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/rj.v19i1.4968

Abstract

Malaria is an infectious disease caused by microorganisms of the genus Plasmodium, transmitted to humans by the female Anopheles mosquito species. The breeding place of this Anopheles mosquito is typically found in the environment, such as stagnant water in rivers, abandoned ponds, lagoons, and other similar areas. Pesawaran Regency is a malaria-endemic area in Lampung Province with an API of 1.4 in 2023. Mapping the distribution of breeding places is necessary to control malaria vectors in Pesawaran Regency. Based on this, this study will conduct a spatial analysis of the breeding sites of Anopheles sp. mosquitoes, which are potential vectors of malaria infection in Pesawaran District. This type of research is descriptive observational using cross sectional design, coordinate survey and visual inspection of larvae found in breading place and spatially processed using Arcgis V.10.8. From the observations of breeding places in the form of swamps, water reservoirs (buckets and derigen) unused fish ponds or ponds, buffering breeding place analysis carried out can be seen that malaria cases are in the area of vector flight distance of 1,000 meters and 3,000 meters. Potential breeding places were found in Teluk Pandan sub-district of Pesawaran Regency, breeding places are scattered in five villages and are located in coastal areas with a distance between 407.88 meters - 707.266 meters from the coastline, buffering analysis of breeding places can be seen that the location at risk of malaria case transmission is in the vector flight distance area of 1,000 meters and 3,000 meters covering eight villages.