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Differences of enamel print patterns between buccal and lingual surfaces of extracted permanent maxillary first premolar Soo, Sheng Cheng; Murniati, Nani; Harsanti, Andriani; Malinda, Yuti; Oscandar, Fahmi
Padjadjaran Journal of Dentistry Vol 32, No 2 (2020): July 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no2.21639

Abstract

Introduction: Ameloglyphics is the study of enamel print patterns on the tooth surface. Enamel print patterns are unique to each individual as they differ on each tooth. The similarity of tooth prints on buccal and lingual surfaces remains questionable as no research has been conducted thus far. The premolar was chosen in this experiment due to its low risk of damage because it is protected by cheek and rarely used compared to other teeth. Thus, the results obtained will be more precise and have higher accuracy than using other teeth. This research was aimed to investigate the differences between enamel print patterns on buccal and lingual surfaces of maxillary right first premolar. Methods: An analytic study was conducted by observing and analysing a total of 34 teeth samples using the purposive sampling method. The middle third of the buccal and lingual tooth surfaces was captured directly after magnified with a stereomicroscope. Photomicrographs of samples were then analysed using SourceAFIS biometric software. This software will process the image and shows patterns of enamel print. Afterwards, the enamel print patterns were observed and classified according to Manjunath Classification. Results: The results suggested that all 34 samples show a similarity of enamel print patterns on both the buccal and lingual surfaces. The interpretations of photomicrographs were statistically analysed using the Statistical Package of Social Science (SPSS) software. The similarity may be due to the same placement of developing tooth bud, temperature, environmental factor, genetics, pressure and nutrition to the ameloblasts cells in a tooth. Conclusion: Both buccal and lingual in the samples show high similarity due to the very high frequencies of similar sub-patterns occur on both surfaces. Tooth print can be used as a valuable tool in forensic science for personal identification with good prospects in the field of forensic dentistry.
The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment Putri, Chany Mony Dwiayu; Prisinda, Diani; Malinda, Yuti
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28638

Abstract

Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.
Effectiveness of ChKM solution compared to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess Malinda, Yuti; Azhara, Dea Hanin; Harnung, Kartikaning; Prisinda, Diani
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28642

Abstract

Introduction: Chronic periapical abscesses are caused by polymicrobial bacteria, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis. These bacteria can be eliminated with chlorophenol camphor menthol (ChKM) solution and triple-antibiotic paste (TAP) as an intracanal medicament. This study compared the effectiveness of ChKM solution to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess. Methods: An experimental laboratory was conducted in-vitro with the microdilution method and optical density measurement using a spectrophotometer. The cell inhibition percentage was calculated to determine the MIC value. The MBC values were confirmed by cultivating the Mueller Hinton Agar samples, incubating them at 37°C for 24 hours, and observing bacterial growth. Bacteria did not grow in the medium at MBC value. The lower MIC and MBC were sensitive and could be an effective medicament choice. Results: The MIC ChKM solution inhibits E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277 were 4000, 4000, 2000, and 2000 µg/mL consecutively. While MIC of TAP were 6, 0.375, 0.75, and 1.5 µg/mL, respectively. Moreover, MBC of the ChKM solution were 32000, 32000, 8000, 8000 µg/mL and MBC of the TAP were 768, 24, 24, 96 µg/mL. Conclusions: ChKM solution and TAP effectively inhibited and killed E. faecalis, S. mutans, S. sanguinis, and P. gingivalis as an intracanal medicament, as seen from the MIC and MBC values. However, TAP is more effective than the ChKM solution because MIC and MBC values of TAP are much lower than the ChKM solution. This finding indicated that TAP is more effective at the lowest concentration than ChKM solution. It should be highlighted that this was an in-vitro study involving specific microbes; thus, further clinical research is needed.
Age estimation of ancient skeletons based on the molar teeth attrition Ramadhani, Triane Ayu; Sarilita, Erli; Malinda, Yuti; Yondri, Lutfi; Cunningham, Craig A; Oscandar, Fahmi
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.222-230

Abstract

Background: Two ancient skeletons (Subang Men) were excavated at the Subanglarang site, Subang Regency, West Java, Indonesia. The partial jaw containing set of teeth from the two skeletons was examined to determine the level of dental attrition present which was then used to estimate age at death.Objective: The aim of this study was therefore to determine the pattern of attrition on the molar teeth and use this to estimate the age at death of the Subang Men.Methods: The pattern of attrition in each cusp of the first molar (M1) and second molar (M2) was examined clinically and then compared with the Average Stage of Attrition (ASA) method. Estimated age was then estimated using formula that are outlined in the Average Stage of Attrition (ASA) method.Results: The results showed that the level of attrition that occurred in the M1 and M2 teeth of the two ancient man was significant. The estimated age at death calculated based on the attrition pattern of molar teeth for skeleton 1 was 38.9 and skeleton 2 was 61.9-64.6.Conclusion: Dental attrition which is caused by physiologic and pathologic teeth friction is related to age. Therefore, age estimation can be determined based on the attrition pattern that occurs in the teeth.
Age estimation of ancient skeletons based on the molar teeth attrition Ramadhani, Triane Ayu; Sarilita, Erli; Malinda, Yuti; Yondri, Lutfi; Cunningham, Craig A; Oscandar, Fahmi
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.222-230

Abstract

Background: Two ancient skeletons (Subang Men) were excavated at the Subanglarang site, Subang Regency, West Java, Indonesia. The partial jaw containing set of teeth from the two skeletons was examined to determine the level of dental attrition present which was then used to estimate age at death.Objective: The aim of this study was therefore to determine the pattern of attrition on the molar teeth and use this to estimate the age at death of the Subang Men.Methods: The pattern of attrition in each cusp of the first molar (M1) and second molar (M2) was examined clinically and then compared with the Average Stage of Attrition (ASA) method. Estimated age was then estimated using formula that are outlined in the Average Stage of Attrition (ASA) method.Results: The results showed that the level of attrition that occurred in the M1 and M2 teeth of the two ancient man was significant. The estimated age at death calculated based on the attrition pattern of molar teeth for skeleton 1 was 38.9 and skeleton 2 was 61.9-64.6.Conclusion: Dental attrition which is caused by physiologic and pathologic teeth friction is related to age. Therefore, age estimation can be determined based on the attrition pattern that occurs in the teeth.
Co-Authors - Azhari Agre Liana Bella Clara Ame Suciati Setiawan Ame Suciati, Ame Andriani Harsanti, Andriani Angrinda Kharisma Putri Aulia Narendra Mohamad Paham Azhara, Dea Hanin Bambang Hidayat Cunningham, Craig A Dewi Zakiawati, Dewi Diani Prisinda Dimas Anugrah Putra Diny Hafizha Amelia Dita Kusuma Wardani Elfiyatinnufus, Rifqiyah Endah Mardiati Endah Mardiati, Endah Erna Herawati Fahmi Oscandar Fahmi Oscandar Fahmi Oscandar, Fahmi Farah Hana Kusumaputri Firstady Widyarnan Munandar Fitri Rahmadhanti Nurfajrina haifa nawari, bilqis Harnung, Kartikaning Hasna Nur Afina Hidayat, Septian Rahmat Hilda Hindasah Hindrya Meidina Fresty I Putu Aditya Widiatama Ibrahim Adilla Indah Suasani Wahyuni Indra Mustika Setia Pribadi Indra Mustika SP, Indra Inne Suherna Sasmita Intan Melani Irene Dewi Kurniawati Janah Eka Widiarni Kancana, Sildha Pura Khairiah, Arifatul Laut, Deru Marah Lusi Epsilawati Lutfi Yondri Lutfi Yondri Melani, Intan Mieke Hemiawati Satari Mieke Hemiawati Satari Munandar, Firstady Widyarnan Murnisari Dardjan Murnisari Dardjan, Murnisari Nanan Nur'aeny Nanan Nur’aeny Nani Murniati Nina Djustiana Nugraha, Alhana Nur, Muhammad Arfianto Nur, Muhammad Arfianto Prasetyo Tri Herlambang Putri, Chany Mony Dwiayu Ramadhani, Triane Ayu Restu Pujiyanti Hidayat Riani Setiadhi Rita Purnamasari Saputra, Sintia Sarilita, Erli Setiadi, Desyani Shalihah, Desyani Sintia Saputra Soo, Sheng Cheng Sri Mulyanti Sri Tjahajawati, Sri Supian, Sudradjat Suryo Adhi Wibowo Syelanisa Nabilla Ulfah Utami Vasya Aulia Wahyu Hidayat Wilam, Christiana Wisam Rizqullah Yoni Fuadah Syukriani Yurika Ambar Lita