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All Journal Journal of Dentistry Indonesia ODONTO Dental Journal Dentino: Jurnal Kedokteran Gigi Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Jurnal Ilmiah FIFO B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Padjadjaran Journal of Dental Researchers and Students eProceedings of Engineering Odonto dental journal Enrichment: Journal of Multidisciplinary Research and Development

Yuti Malinda

Departemen Oral Biologi, Fakultas Kedokteran Gigi - Rumah Sakit Gigi Dan Mulut, Universitas Padjadjaran, Indonesia

Author-ID : 614347

Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences

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Comparison of chlorine dioxide and chlorhexidine 2% antiseptic in reducing bacterial colony counts as an alternative to DUWLs cleaning: a quasi-experimental study Elfiyatinnufus, Rifqiyah; Mulyanti, Sri; Utami, Ulfah; Malinda, Yuti; Laut, Deru Marah
Padjadjaran Journal of Dentistry Vol 35, No 3 (2023): November 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no3.48357

ABSTRACTIntroduction: The inner surfaces of dental unit waterlines (DUWLs) possess an accumulation of any bacteria. Microorganisms can enter dental unit waterlines from water reservoirs. Antiseptics are substances that inhibit the growth of bacteria. Chlorine dioxide is effective in decontaminating microbes in the DUWLs and has a beneficial effect on reducing nosocomial infections. Chlorhexidine effectively prevents the growth of Streptococcus bacteria. The addition of antiseptic agents to the water source contributed to a significant reduction of the cultivable microbial counts in the aerosol. The purpose of this study was to analyze the difference between Chlorine Dioxide 0,1% and Chlorhexidine 2% antiseptics on the number of bacterial colonies in the Dental Unit Waterline. Methods: This study was a quasi-experimental study with a total sample size of 8 dental units that have water tanks in the oral surgery clinic. The sampling technique was total sampling, where the sample was divided into 2 groups, group I using Chlorine Dioxide and group II using 2% Chlorhexidine calculation of colony counts unit using the plate count method. Results: The difference in the number of colonies before being given Chlorine Dioxide was 13,153 CFU/mL and after being given Chlorine Dioxide antiseptic was 6,070 CFU/mL, while before being given 2% Chlorhexidine antiseptic was 12,917 CFU/mL and after being given 2% Chlorhexidine antiseptic was 2,823 CFU/mL. There is a significant difference in the number of bacterial colonies before and after being given Chlorine Dioxide and Chlorhexidine 2% antiseptic with ρ=0.001; Conclusion: Chlorhexidine 2% reduces bacterial colony forming unit in DUWLs much more than using Chlorine dioxide for the alternative of DUWLs Cleaning Agents.Keywords: DUWLs, chlorine dioxide, chlorhexidine 2%, bacterial colony counts

Effectiveness of Calcium Hydroxide Against Enterococcus Faecalis Biofilm in Dental Root Canals haifa nawari, bilqis; Malinda, Yuti; Prisinda, Diani
Enrichment: Journal of Multidisciplinary Research and Development Vol. 2 No. 11 (2025): Enrichment: Journal of Multidisciplinary Research and Development
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/enrichment.v2i11.300

Enterococcus faecalis (E. faecalis) is the most widely detected species associated with persistent endodontic infections because it has the ability to form biofilms. This study aimed to identify the effectiveness of calcium hydroxide paste Calcigel® (Prevest DenPro, India) on E. faecalis (ATCC 29212) biofilms in root canals with exposure times of 14, 21, and 28 days. The type of research used was true experimental in vitro by randomly selecting samples of tooth roots on the 14th day of biofilm development and dividing them into six groups: use of Ca(OH)2 as intra-canal medicament for 14, 21, and 28 days, as well as positive control without treatment for 14, 21, and 28 days. Dentin chip suspensions were used for colony form ng units counting to estimates remaining E. faecalis counts. The effectiveness calculation obtained by comparing the test group with the control group at the same treatment duration, with the results of using Ca(OH)2 for 14 days showing a decrease in the number of E. faecalis 96.46%, the results of using Ca(OH)2 for 21 days was 95.20%, while the results of using Ca(OH)2 for 28 days was 94.59%. The results of testing the hypothesis with the ANOVA test showed that there was no significant difference between the effectiveness of calcium hydroxide with exposure to 14 days, 21 days, and 28 days with a significance value of <0.452.

Description of palatal rugae size and direction in children with gender difference Nur, Muhammad Arfianto; Djustiana, Nina; Malinda, Yuti
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Universitas Padjadjaran

Introduction: Indonesia has a high number of children victims of disaster in the last five years, but the children do not have identity cards, causing difficulties in the identification process. Palatal rugae are considered unique in individuals, and their morphology remains stable throughout life. Data of palatal rugae pattern in children aimed to facilitate the process of identification of the child's gender. The purpose of this research was to discover the description of palatal rugae in children which can be used as a reference of gender identification in children. Method: A descriptive research design was performed with purposive sampling method. Samples were taken from 119 maxilla images consisted of 69 images of girls maxilla and 50 images of boys maxilla. The study was performed by taking individual maxilla images with intraoral mirror and observing towards the direction of the palatal rugae according to the Carrea classification, and the size of palatal rugae according the Lysell classification. Results: The type IV direction and secondary size of palatal rugae were found to be dominant on both sides of maxilla in boys and girls. Conclusion: Palatal rugae in boys and girls were having the same direction which was the type IV, and the size of secondary rugae was found to be dominant on both sides of the palate. Palatal rugae also proofed to be able to become the complementary method for determining unidentified gender in children.

Human age estimation based on pulp volume of canines for chronological age estimation: Preliminary research Hidayat, Septian Rahmat; Oscandar, Fahmi; Malinda, Yuti; Sasmita, Inne Suherna; Dardjan, Murnisari; Murniati, Nani; Lita, Yurika Ambar
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Introduction: Age estimation has an important role in the process of human identification and needs to be performed in an efficient, fast, and accurate ways. Age estimation based on the pulp cavity volume of canines was referring to the theory stated that the apposition of secondary dentine during human life could be determined as an age indicator. This study was aimed to estimate the human age based on the pulp volume of canines for chronological estimation. Methods: Forty-one canine teeth from Cone Beam Computed Tomography (CBCT) 3D image sample from Dental Hospital of the Faculty of Dentistry Universitas Padjadjaran segmented into dental pulp and enamel were prepared for measurement of pulp volume using the ITK SNAP volumetric software. Descriptive analysis of pulp volume and regression equations were generated from regression analysis of pulp volume and tested for age estimation. Result: Dentine thickness was found to be increased from adolescence age range until adulthood age range. Logarithmic regression between pulp volume was statistically significant (p < 0,001). The coefficient of determination (R2) was 0.753 with a standard deviation of 8.421. Mathematics model for estimating age was: Age = 79.523 – (18.194 x ln(Pulp Volume)). The pulp cavity and dentine thickness were decreasing along the age. The same equation was also used in the same sample. There were several differences between chronological age and estimated age approximately 8.4 years following the regression result of R2 (75.3%). The rest of the sample (24.7%) have a big difference, and this could be affected by modifying variables such as occlusal force and human error measurement procedures. Conclusion: The human age can be estimated by the pulp volume of canines.Keywords: Age estimation, pulp volume, canine, ITK-SNAP, CBCT.

Diagnosis determination of chronic periapical abscess case using imaging radiography software and microbiological examination in female patients Prisinda, Diani; Malinda, Yuti; Lita, Yurika Ambar; Tjahajawati, Sri
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Diagnosis determination of chronic periapical abscess can be achieved by subjective, objective, and other examination such as radiographic and microbiological examination based on clinical finding and differences intensity at the periapical area with healthy bone suspected to be chronic abscess periapical. A microbiological examination performed to obtain the predominant bacteria that involved to determine an adequate medicament to succeed endodontic treatment. This study was aimed to compare intensity abscess lesion segmentation image reconstruction and surrounding bone for determine the diagnosis of periapical abscess more accurately and to determine the characteristic of bacteria colony from a periapical abscess tooth. Methods: The study sampling method was consecutive sampling (6 female) consisted of inclusion criteria patient with a periapical lesion in anterior maxillary that diagnosed based on clinical signs and symptoms. CBCT 3D scan of the maxillofacial region taken from of patients was measured image intensity periapical lesion and surrounding bone using semi-auto segmentation with ITK-SNAP 3.6.0. Samples from the root canals taken using three sequential sterile paper points. The samples were plated, and microorganisms were then isolated and identiﬁed by the colony characteristics. Results: There was a signiﬁcant difference between the mean content of lesion intensity of (n = 6,256.710 ± 81.930 and 636.022 ± 79.981 lesion boundary. The two-tailed p-value = 0.0050, this difference was considered to be very statistically significant. The mean of group lesion minus group surrounding bone equals to 163.715. 95% confidence interval of this difference from 75.542 to 251.888. Conclusions: Demineralisation of periapical bone can be determined by voxel intensity value in CBCT 3D radiograph using semi-auto segmentation ITK SNAP, could be recommended as a quantitative interpretation for detection periapical lesion and chronic periapical abscesses is a poly-microbial disease. Keywords: Chronic periapical abscess, endodontic, microbiology, radiograph CBCT

Acquaintance of bite mark identification procedures in Forensic Odontology Malinda, Yuti; Zakiawati, Dewi
Padjadjaran Journal of Dentistry Vol 27, No 3 (2015): November 2015
Publisher : Universitas Padjadjaran

Introduction: Bite mark analysis casework strives to connect a biter to the teeth pattern present on the object linked in some way to crime or event. This analysis requiring an immediate response by the forensic odontologist since the marks fade rapidly in the living and the dead in a matter of hours. The aim of this article is to help the dentist to know and understand the procedures of bite mark identification in forensic odontology field. Literature review: Bite marks may be present the following situations, a fight between adults or children, a part of sexual or physical assault by adult on children, a rape, and homosexual activities. The marks can be single or multiple, varying degrees of severity from mild marking of the tissue to deep perforation, varying location may be found on breast, face/ head, abdomen, shoulder, upper extremity, buttocks, female genitalia, male genitalia, legs, ear, nose and neck. Discussion: Dentist should master the bite mark analysis procedures. When the suspect has been recognized, the first thing to do is swabbing the oral mucosa, and then taking photographs. The next steps are preparing to take impression, and always taking notes about the procedure and the result. A soon as all the documents are completed, do the matching procedures. Delay examination will cause the lost of valuable evidence. Conclution: Dentist should carefully determine the conclusion among these possibilities , the mark is “possible biter”, “probable biter”, or “with a high level of confidence, is the biter”

Differences of enamel print patterns between buccal and lingual surfaces of extracted permanent maxillary first premolar Soo, Sheng Cheng; Murniati, Nani; Harsanti, Andriani; Malinda, Yuti; Oscandar, Fahmi
Padjadjaran Journal of Dentistry Vol 32, No 2 (2020): July 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no2.21639

Introduction: Ameloglyphics is the study of enamel print patterns on the tooth surface. Enamel print patterns are unique to each individual as they differ on each tooth. The similarity of tooth prints on buccal and lingual surfaces remains questionable as no research has been conducted thus far. The premolar was chosen in this experiment due to its low risk of damage because it is protected by cheek and rarely used compared to other teeth. Thus, the results obtained will be more precise and have higher accuracy than using other teeth. This research was aimed to investigate the differences between enamel print patterns on buccal and lingual surfaces of maxillary right first premolar. Methods: An analytic study was conducted by observing and analysing a total of 34 teeth samples using the purposive sampling method. The middle third of the buccal and lingual tooth surfaces was captured directly after magnified with a stereomicroscope. Photomicrographs of samples were then analysed using SourceAFIS biometric software. This software will process the image and shows patterns of enamel print. Afterwards, the enamel print patterns were observed and classified according to Manjunath Classification. Results: The results suggested that all 34 samples show a similarity of enamel print patterns on both the buccal and lingual surfaces. The interpretations of photomicrographs were statistically analysed using the Statistical Package of Social Science (SPSS) software. The similarity may be due to the same placement of developing tooth bud, temperature, environmental factor, genetics, pressure and nutrition to the ameloblasts cells in a tooth. Conclusion: Both buccal and lingual in the samples show high similarity due to the very high frequencies of similar sub-patterns occur on both surfaces. Tooth print can be used as a valuable tool in forensic science for personal identification with good prospects in the field of forensic dentistry.

The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment Putri, Chany Mony Dwiayu; Prisinda, Diani; Malinda, Yuti
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28638

Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.

Effectiveness of ChKM solution compared to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess Malinda, Yuti; Azhara, Dea Hanin; Harnung, Kartikaning; Prisinda, Diani
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28642

Introduction: Chronic periapical abscesses are caused by polymicrobial bacteria, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis. These bacteria can be eliminated with chlorophenol camphor menthol (ChKM) solution and triple-antibiotic paste (TAP) as an intracanal medicament. This study compared the effectiveness of ChKM solution to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess. Methods: An experimental laboratory was conducted in-vitro with the microdilution method and optical density measurement using a spectrophotometer. The cell inhibition percentage was calculated to determine the MIC value. The MBC values were confirmed by cultivating the Mueller Hinton Agar samples, incubating them at 37°C for 24 hours, and observing bacterial growth. Bacteria did not grow in the medium at MBC value. The lower MIC and MBC were sensitive and could be an effective medicament choice. Results: The MIC ChKM solution inhibits E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277 were 4000, 4000, 2000, and 2000 µg/mL consecutively. While MIC of TAP were 6, 0.375, 0.75, and 1.5 µg/mL, respectively. Moreover, MBC of the ChKM solution were 32000, 32000, 8000, 8000 µg/mL and MBC of the TAP were 768, 24, 24, 96 µg/mL. Conclusions: ChKM solution and TAP effectively inhibited and killed E. faecalis, S. mutans, S. sanguinis, and P. gingivalis as an intracanal medicament, as seen from the MIC and MBC values. However, TAP is more effective than the ChKM solution because MIC and MBC values of TAP are much lower than the ChKM solution. This finding indicated that TAP is more effective at the lowest concentration than ChKM solution. It should be highlighted that this was an in-vitro study involving specific microbes; thus, further clinical research is needed.

Age estimation of ancient skeletons based on the molar teeth attrition Ramadhani, Triane Ayu; Sarilita, Erli; Malinda, Yuti; Yondri, Lutfi; Cunningham, Craig A; Oscandar, Fahmi
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.222-230

Background: Two ancient skeletons (Subang Men) were excavated at the Subanglarang site, Subang Regency, West Java, Indonesia. The partial jaw containing set of teeth from the two skeletons was examined to determine the level of dental attrition present which was then used to estimate age at death.Objective: The aim of this study was therefore to determine the pattern of attrition on the molar teeth and use this to estimate the age at death of the Subang Men.Methods: The pattern of attrition in each cusp of the first molar (M1) and second molar (M2) was examined clinically and then compared with the Average Stage of Attrition (ASA) method. Estimated age was then estimated using formula that are outlined in the Average Stage of Attrition (ASA) method.Results: The results showed that the level of attrition that occurred in the M1 and M2 teeth of the two ancient man was significant. The estimated age at death calculated based on the attrition pattern of molar teeth for skeleton 1 was 38.9 and skeleton 2 was 61.9-64.6.Conclusion: Dental attrition which is caused by physiologic and pathologic teeth friction is related to age. Therefore, age estimation can be determined based on the attrition pattern that occurs in the teeth.

Co-Authors - Azhari Agre Liana Bella Clara Ame Suciati Setiawan Ame Suciati, Ame Andriani Harsanti, Andriani Angrinda Kharisma Putri Azhara, Dea Hanin Bambang Hidayat Cunningham, Craig A Dewi Zakiawati, Dewi Diani Prisinda Dimas Anugrah Putra Diny Hafizha Amelia Dita Kusuma Wardani Elfiyatinnufus, Rifqiyah Endah Mardiati Endah Mardiati, Endah Erna Herawati Fahmi Oscandar Fahmi Oscandar Fahmi Oscandar, Fahmi Farah Hana Kusumaputri Firstady Widyarnan Munandar Fitri Rahmadhanti Nurfajrina haifa nawari, bilqis Harnung, Kartikaning Hasna Nur Afina Hidayat, Septian Rahmat Hilda Hindasah Hindrya Meidina Fresty I Putu Aditya Widiatama Ibrahim Adilla Indah Suasani Wahyuni Indra Mustika Setia Pribadi Indra Mustika SP, Indra Inne Suherna Sasmita Intan Melani Irene Dewi Kurniawati Janah Eka Widiarni Kancana, Sildha Pura Khairiah, Arifatul Laut, Deru Marah Lusi Epsilawati Lutfi Yondri Lutfi Yondri Melani, Intan Mieke Hemiawati Satari Mieke Hemiawati Satari Munandar, Firstady Widyarnan Murnisari Dardjan Murnisari Dardjan, Murnisari Nanan Nur'aeny Nanan Nur’aeny Nani Murniati Nina Djustiana Nugraha, Alhana Nur, Muhammad Arfianto Nur, Muhammad Arfianto Paham, Aulia Narendra Mohamad Prasetyo Tri Herlambang Putri, Chany Mony Dwiayu Ramadhani, Triane Ayu Restu Pujiyanti Hidayat Riani Setiadhi Rita Purnamasari Salsabila, Syifa Ainun Fatiha Saputra, Sintia Sarilita, Erli Setiadi, Desyani Shalihah, Desyani Sintia Saputra Soo, Sheng Cheng Sri Mulyanti Sri Tjahajawati, Sri Supian, Sudradjat Suryo Adhi Wibowo Syafiq, Ikram Syelanisa Nabilla Ulfah Utami Vasya Aulia Wahyu Hidayat Wilam, Christiana Wisam Rizqullah Yoni Fuadah Syukriani Yurika Ambar Lita

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