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SKRINING FITOKIMIA DAN UJI ANTIMIKROBA EKSTRAK KASAR BAWANG BATAK (Allium cinense) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli Siti Rubiatik; Sartini Sartini; Rosliana Lubis
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 2, No 1 (2015): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v2i1.755

Abstract

The purpose of this research is to determine the composition of secondary metabolites of crude extract “bawangbatak” (Allium  cinense) and anti-microbial properties of the extract against Staphylococcus aureus and Escherichia coli. This research was conducted in three (3) stage, stage I  by the manufacturing of crude extract of garlic bulbs “bawangbatak”, Allium cinense, through maceration method using a solvent n-hexane and methanol. In stage II, screening fitokimia, that is identification of secondary metabolites (alkaloids, flavonoids, terpenoids and steroids) to the crude extract of tuber “bawangbatak”, Allium cinense, and phase III, test bioktifitas crude extract bulb of  “bawangbatak” Allium cinense in the form of extracts antimicrobial properties against Staphylococcus aureus and Escherichia coli extract using the concentration variation of 0%, 1%, 5%, 15%, 20%, and 25% and incubation at 370C for 24 hours. The results showed that secondary compounds was dominated by flavonoids, steroids, terpenoid and tannins. While at the test screening phytochemicals extra n-hexane showed no secondary metabolites, test bioktivitas against bacteria have inhibitory at a concentration of 15% with inhibition zone 14 mm in bacteria Escheriachia coli and 10 mm in bacteria Staphylococcus aureus this indicates onion extract  of “bawangbatak”  has the ability as an anti-microbial.
PEMANFAATAN KITOSAN DARI LIMBAH CANGKANG KERANG BULU(Anadara inflata) SEBAGAI BAHAN PENJERNIH AIR SUNGAING DARI LIMBAH KULIT DURIAN Rosliana Lubis; Muhammad Usman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 1, No 1 (2014): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v1i1.16

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The title of  this study is the utilization of chitosan from shellfish waste as water purifier. The purpose of the study is to utilize coagulant of shellfish waste in water treatment proes into clear water. The research variabel are the concentration of chitosan 1,3,5,7, and 9 g/l. The particle size of chitosan used in this research is 150 mesh. The time consume  8 hours for purification process. The result showed that 9 g/l chitosan shell waste is effective to water purification process. At a concentration of 9 g/l. Chitosan shellfish waste can produce clean water in complianee with health minister No. 416/MENKES/PER/IX/1990 about  water quality treatment.
ANALISIS KANDUNGAN LOGAM Pb, Cu, Cd DAN Zn PADA SAYURAN SAWI, KANGKUNG DAN BAYAM DI AREAL PERTANIAN DAN INDUSTRI DESA PAYA RUMPUT TITIPAPAN MEDAN Muhammad Yusuf; Kiki Nurtjahja; Rosliana Lubis
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 3, No 1 (2016): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v3i1.812

Abstract

The levels of Pb, Cu, Cd and Zn in sawi (Barsssica rapa), ground kuna (Ipomea reptans), and spinach (Amaranthus tricolor) in the area of Paya Rumput Titipapan Medan village have been done. The goal is to know the levels of these metals in vegetables grown in the agricultural area of Paya Rumput Titipapan Village. Samples taken randomly in wash drained and weighed. Wet weight then dried at a temperature of 50-100o C for 3 hours, After dry on weigh in gerus until smooth. The sample was destructed with concentrated nitric acid with temperature 80o C, the sample was analyzed by using atomic absorption spectrophotometric (AAS). The results showed the highest levels of lead found in spinach is 6 mg / kg. Levels of cadmium in kale and spinach is 3 mg / kg. While the Cu content on the analysis results are still within the maximum limit. The concentration of Pb, Cd, Zn in each sample exceeds the maximum allowed by the Decree of Dit Jend POM No 03725 / B / SKVII / 89 and SNI 7387: 2009.
Skrining Fitokimia dan Antimikroba Ekstrak Daun Kirinyuh terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli Nuriana Munte; Sartini sartini; Rosliana Lubis
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 2, No 2 (2016): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v2i2.803

Abstract

This reseach aims to determine the composition of secondary metabolites of kirinyuh leaf extract (Euphatorium ordoratum.L) and antimicrobial properties of the extract against Staphylococcus aureus and Escherichia coli. The study was conducted in three phases: the first phase is manufacture kirinyuh leaf extract (Euphatprium odoratum L) through maceration method using N-Hexane solvent and methanol. In stage II, phytochemical screening, namely the identification of secondary metabolites (alkaloids, flavonoids, terpenoids and steroids) to the crude extract of  leaves kirinyuh (Ephatorium odoratum L) in the form of antimicrobial properties of extracts against Staphylococcus aureus and with variations extract concentrations of 0%, 1 %, 5%, 10%, 15%, 20%, and 25% Echerichia coli and incubated at 37°C for 24 hours. The research found that such compounds secondary metbolit alkaloids, flavonoids, steronoid, and saponin, which dominates the secondary metabolites of four that alkaloid. While the screening test of phytochemical N-hexane extracts is not contain metbolit secondary compounds, testing against bacteria possess bioktivitas inhibition at concentrations of 15% with inhibition zone of 1.3 cm on Escherichia coli bacteria and 1.0 cm in bacteria Staphylococcus aureas. This shows kirinyuh leaf extracts have the ability as an anti-microbial.
Marine Ecolexicon of Noun-Verb of the Coast Community in Pesisir Barus, Central Tapanuli Rosliana Lubis; Dwi Widayati
JURNAL ARBITRER Vol. 8 No. 1 (2021)
Publisher : Masyarakat Linguistik Indonesia Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/ar.8.1.82-92.2021

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This study aims to describe the lingual form, which is a manifestation of the understanding of the speech community of the environment towards its environmental dimension. The theory used in this research is ecolinguistic. Data in the form of basic lexicons and affixed lexicons related to the marine environment were collected through interviews with informants. Furthermore, the data were analyzed by grouping them based on the word class, environmental category, and the affixation process contained in the affixed words. The results of the study show that many marine environmental lexicons which are divided into noun lexicon and verb lexicon. The noun lexicon is divided into four lexicon categories, namely: (1) Marine Environmental Fauna Lexicon (88 lexicons); (2) Flora of the Marine Environment Flora (9 lexicons); (3) Lexicon of Facilities / Infrastructure for Marine Environmental Activities (16 lexicons); and (4) Nominal Environmental Lexicon (7 lexicons). The number of vocabulary that is still recorded in the cognition of the Barus coastal Malay language community indicates that the community is very familiar with its environment and therefore the vocabularies are preserved.
Penentuan Kadar Glukosa Urine di Laboratorium Rumah Sakit Sari Mutiara Medan Fitri Dian Puspa Nadeak; Riyanto riyanto; Rosliana Lubis
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 1, No 2 (2019): November 2019
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.444 KB) | DOI: 10.31289/jibioma.v1i2.161

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The purpose of this study was to determine urine glucose level in patients who came to the Sari Mutiara General Hospital in Medan. Research method with qualitative and quantitative test, qualitative analysis of urine glucose by inserting urine sample 5 - 8 drops into reaction tube that already contains 5ml of benedict solution, then burned on fire and observe the color change that occurs, quantitative analysis of urine glucose by adding sodium carbonate and 2 rounds of boiling stone into a reaction tube containing 5 ml of benedict solution, burned over a flame, then drop urine using a 1 ml pipette, while dripping urine, the liquid should not stop boiling, the titration ends when the blue is not look again.The results of qualitative and quantitative analysis were obtained from 25 test samples, 15 positive urine glucose patients, and 10 negative urine glucose patients.
Penentuan Kadar Kalium Iodat (KIO3) dalam Garam Konsumsi yang Beredar Dipasaran dengan Metode Iodometri Muthiah Muthiah; Rosliana Lubis; Riyanto Riyanto
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 1 (2020): Mei 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.021 KB) | DOI: 10.31289/jibioma.v2i1.186

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Potassium iodide is one of the substances that must be present in iodized salt. The purpose of this research was to determine the level of KIO3 in consumption salts that are circulating in the market by iodometry method based on Indonesia National Standart 01-3556-2016. The results of the reseacrh showed that of the 15 salt sampels, it turns out that 8 salt samples of KIO3 levels meet the SNI standard, which is 30 – 80 ppm, while 7 salt sampels did not meet the SNI Standard. For 7 salt sampels that did not meet SNI standard after testing the moisture content, sodium chloride content and cadmium contamination, only 3 salt sampels of water content meet the SNI standard and 4 sampels of water content did not meet it.For testing the levels of sodium chloride and cadmium contamination, it has met SNI standards.
Analisa Antifungal Ekstrak Etanol Biji Alpukat Terhadap Pertumbuhan Jamur Colletotrichum sp. pada Cabai Rawit (Capsicum Frutescens) Muhammad Ferdiansyah; Jamilah Nasution; Rosliana Lubis
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 1 (2020): Mei 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.252 KB) | DOI: 10.31289/jibioma.v2i1.182

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This study aimed to assess the potential of avocado seed extract as an antifungal agent and to determine the optimum concentration of avocado seed extract in inhibiting the growth of Colletotrichum. This research used laboratory- scale experimental methods. Inhibition test of extract towards Colletotrichum was carried out using poison food method using following extract concentration 20%, 40%, 60%, 80% and 100% Topsin M Wp 0.2% were used as negatif control. The result showed that avocado seed extract had a significant antifungal activity against Colletotrichum at the consentration of 40%, the effect was emerged since the 4th day of incubation which was observed by measuring fungal growth diameter
Identifikasi Senyawa Metabolit Sekunder pada Durian (Durio zibethinus Murr) Aji Suteja; Emmy Harso Kardhinata; Rosliana Lubis
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 1, No 1 (2019): Mei 2019
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.808 KB) | DOI: 10.31289/jibioma.v1i1.138

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This study aims to determine how to identify the types of secondary metabolite compounds contained in durian leaves. The study was conducted by an experimental method by identifying secondary metabolite compounds on durian leaves using phytochemical screening. The sample criteria used were the leaf buds and all the leaf strands (except the leaf bones). The results of the study showed that of the three types of durian leaves namely copper, baskets and Sp A. There are several types of secondary metabolite compounds including alkaloids, steroids, and terpenoids. Phytochemical screening analysis was carried out using thin layer chromatography by showing RF values on three types of durian leaves using methanol and ethyl acetate solvents in a ratio of 3: 1. RF value on copper durian is 0.97, bakul durian is 0.95 and Sp durian. A 0.94.
Influence of monomer concentration on the morphology, contact angle, water uptake, and antibacterial activity of grafted cellulose obtained from peel durian ROSLIANA LUBIS; SAISA SAISA
Jurnal Natural Volume 22 Number 1, February 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1963.855 KB) | DOI: 10.24815/jn.v22i1.23865

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The objective of the current study is to evaluate and determine the effect of monomer concentration of ClAETA on the grafting copolymerization of oxidized cellulose which isolated from peel durian. Four concentrations of ClAETA were used in this study, e.g., 40, 50, 60, and 70%. Several analyses were performed to determine the material characteristic, e.g., degree of grafting, FT-IR, surface morphology, contact angle, degree of swelling, and antibacterial activity. The result showed, monomer concetration plays a significant role to the physical and surface morphology of DAC-ClAETA. The degree of grafting of ClAETA was found in the range of 15-31%, and at the concentration of 50% the grafted oxidized cellulose has 30.4% of degree of grafting. SEM images showed the pore volume has linear correlation to the monomer concetration, it increased the surface roughness. Also, the contact angle confirmed the hydrophilicity and degree of swelling of grafted oxidized cellulose was increase that linear to ClAETA concentration. DAC-g- ClAETA was found antibacterial active against S. aureus, S. epidermis, and C. albicans than DAC.