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All Journal BIOMA : Jurnal Biologi Makassar Pedagogik Journal of Islamic Elementary School JURNAL PENDIDIKAN TAMBUSAI Jurnal Studi Guru dan Pembelajaran Jurnal Kesehatan Panrita Husada Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Diponegoro International Medical Journal (DIMJ) Agrimansion: Agribusiness Management & Extension Jurnal Abdimas Indonesia : Jurnal Abdimas Indonesia INSECTA: Integrative Science Education and Teaching Activity Journal Juwara : Jurnal Wawasan dan Aksara Indo-MathEdu Intellectuals Journal Jurnal ABDIMAS Panrita Formosa Journal of Science and Technology (FJST) Formosa Journal of Sustainable Research (FJSR) Journal of Educational Analytics Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi Journal of Learning Improvement and Lesson Study Jurnal TLM Blood Smear PharmaCine : Journal of Pharmacy, Medical and Health Science MULTIPLE: Journal of Global and Multidisciplinary FASKES : Jurnal Farmasi, Kesehatan, dan Sains Satera: Jurnal Sains dan Teknik Terapan Chemedu: Jurnal Ilmiah Pendidikan Kimia Jurnal Medisains Kesehatan Abdi Kimia : Jurnal Pengabdian Masyarakat Macca: Science-Edu Journal Hayyan Journal Jurnal Pengabdian Masyarakat Journal of Multidiscipline and Collaboration Research Frontiers in Sustainable Science and Technology
Islawati
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences (FMIPA), Universitas Negeri Makassar
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Journal : Jurnal Kesehatan Panrita Husada

 1 
PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI Dzikra Arwie; Islawati
Jurnal Kesehatan Panrita Husada Vol 3 No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v3i2.188

Abstract

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.
Ekstrak Betasianin dari Umbi Bit (Beta vulgaris) sebagai Pewarna Alami pada Sediaan Apusan Darah Tepi Islawati; Asriyani Ridwan; Rahmat Aryandi
Jurnal Kesehatan Panrita Husada Vol 6 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v6i2.644

Abstract

ABSTRAK Morfologi sel berupa eritrosit, trombosit dan leukosit merupakan bagian dari pemeriksaan Apusan Darah Tepi (ADT). Pemeriksaan ini mampu mengindetifikasi jenis dan jumlah dari leukosit dan trombosit bahkan mampu mengetahui adanya parasite. Di Indnesia yang memiliki iklim tropis, pewarnaan yang sering digunakan adalah pewarnaan Romanowsky yakni pewarnaan dengan menggunakan pewarnaan wright, giemsa, atau bahkan kombinasi dari kedua pewarnaan tersebut. Kualitas dari pewarnaan apusan darah tepi dapat dinilai dari morfologi eosinophil. Hal ini disebabkan karena kekhasan dari eosinophil serta jumlah yang cukup banyak dan mudah diamati. Selain itu, jaminan dari kevalidan suatu pemeriksaan ADT adalah kualitas dari hasil pewarnaannya. Tujuan dari penelitian ini adalah teramatinya morfolofi dari sel darah dari sediaan apusan darah tepi dengan menggunakan ekstrak betasianin dari umbi bit (Beta vulgaris) sebagai bahan pewarna alami pengganti giemsa. Jenis penelitian berupa eksperimen laboratory dengan metode perbandingan hasil pengamatan terhadap morfologi sel darah yang berupa eritrosit, trombosit dan leukosit dengan menggunakan giemsa sebagai gold standard dan ekstrak betasianin sebagai pewarna alami dari umbi bit. Penelitian ini menggunakan sampel darah vena yang diambil dan ditampung pada tabung EDTA lalu dibuat dalam bentuk sediaan apusan darah. Hasil pewarnaan dengan menggunakan giemsa menunjukkan granula terlihat cukup jelas dan terlihat merah muda dengan inti sel berwarna biru keunguan. ekstrak betasianin menunjukkan bahwa kualitas pewarnaan pada leukosit dan trombosit kurang baik dan eritrosit memiliki kualitas pewarnaan yang cukup baik dengan memperlihatkan warna oranye yang cukup jelas. Sehingga dapat ditarik kesimpulan bahwa ekstrak betasianin dari umbi bit dapat dijadikan sebagai alternative pewarna alami untuk sel eritrosit dari sediaan apusan darah tepi. Kata Kunci: Betasianin, Umbi Bit, Eritrosit, Pewarnaan ABSTRACT Morphology cell in the form of erythrocytes, platelets and leukocytes is part of the peripheral blood smear (ADT) examination. This examination is able to identify the type and number of leukocytes and platelets and is even able to detect the presence of parasites. In Indonesia, which has a tropical climate, the coloring that is often used is Romanowsky staining, namely staining using wright, Giemsa, or even a combination of the two colorings. The quality of the staining of the blood smear can be assessed from the morphology of the eosinophils. This is due to the uniqueness of eosinophils and their large number and easy to observe. In addition, the guarantee of the validity of an ADT examination is the quality of the staining results. The purpose of this study was to observe the morphology of blood cells from peripheral blood smears using betacyanin extract from beetroot (Beta vulgaris) as a natural dye substitute for Giemsa. The type of experimental research is in the form of a laboratory with a comparison method of observations on the morphology of blood cells in the form of erythrocytes, platelets and leukocytes using Giemsa as the gold standard and betacyanin extract as a natural dye from beetroot. This study used venous blood samples taken and stored in an EDTA tube and then made in the form of a blood smear. Staining results using Giemsa showed the granules were quite clear and looked pink with purplish-blue cell nuclei. Betacyanin extract showed that the quality of staining on leukocytes and platelets was not good and erythrocytes had a fairly good staining quality with a fairly clear color range. So it can be concluded that betacyanin extract from beetroot can be used as a natural alternative dye for erythrocyte cells from peripheral blood smear preparations. Keywords: Betacyanin, beetroot, erythrocytes, staining
Pemanfaatan Chitosan Dari Limbah Kulit Udang Sebagai Pestabil Pada Nanopartikel Perak Dengan Bioreduktor Daun Serai (Cymbopogon citratus) Fatimah; Islawati; Asdinar; AR.Pratiwi Hasanuddin
Jurnal Kesehatan Panrita Husada Vol 6 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v6i2.720

Abstract

This study was based on the background Renewable research in nanotechnology has shown the creation of new, better performing products. Nanoparticles are part of nanotechnology which plays an important role in the development of science, especially its application in various fields including the health sector Chitosan has been widely applied commercially in the chemical, food and pharmaceutical industries. Chitosan can be a stabilizer alternative in the formation of silver nanoparticles.The purpose of this study was to determine the use of chitosan from shrimp shell waste as a stablizer on silver nanoparticles with lemongrass leaf as bioreductor. This research is a type of experimental research. Experiments were carried out to determine the ability of chitosan from shrimp shell waste as a stabilizer in silver nanoparticles with lemongrass leaf as bioreductor. Based on the results of the research, the spectrum shows that the silver nanoparticles without the addition of chitosan are formed at a maximum wavelength of 442 nm - 448 nm, while for silver nanoparticles with the addition of chitosan are at a wavelength of 458 nm - 470 nm. The conclusion Chitosan from shrimp shell waste can be used as a stabilizer in silver nanoparticles with lemongrass leaf as bioreductor with a maximum wavelength of 470 nm with a synthesis time of up to 72 hours with an absorbance value of 0.610 nm.
Co-Authors A.R. Pratiwi Hasanuddin AB, Azsrul Adam Adam Afriani Nur Ainul Fira Al Baihaq, Muhammad Raihan Alimin Alimin, Alimin Andi Harmawati Novriani HS Army Auliah Artati Asdinar Asnidar Asnidar Asnidar Asriani Ridwan Asyahra, St. Fatima Awalia, Rizki B, Artika Cahyani, Vika Darma Dewi B , Ade Risma Zagina Dewiyanti Fadly Dzikra Arwie, Dzikra Elfira Jumrah Erni Rismawanti Faathir Almur Fandi Ahmad Fandi Ahmad Fatimah Fatimah Febrianti, Nining Fikroh, Retno Aliyatul Fitriadi Hardin Hasbi Hasmidar, Hasmidar Hujemiati Idaryanti JUMARDI Khusnul Fahima, Aufiq Lukmanul Hakim Samada, Lukmanul Hakim Samada Maryono Muhammad Yunus Muhammad Zainal Arifin, Muhammad Zainal Munawwarah Munawwarah Munawwarah Munawwarah, Munawwarah Muriyati Murni Djabar Murniati, A. Mus, Rosdiana Nur Aina Nur Indah Sari NURLAELA NURLAELA NURUL HIDAYAH Nurul Ilmi, Nurul Ilmi Oktaviyani , Tariska Inka Priyadharmadi Purnama, Andry Purnama, Andry Priyadharmadi Rahmat Aryandi Rahmat Aryandi Rahmat Aryandi Aryandi Rahmi Faradisya Ekapti, Rahmi Faradisya Ekapti Rasul, Rasul Resky Dwijayanti Riska Andriani Riskiyani Risnawati Risnawati Ruad, Inna Raehana Safruddin Salnus, Subakir Samsul, Wahyu Musdalifah Samsul Sitti Hadijah, Sitti Subakhir Salnus Sugeng, Santoso Sugiarti Sugiarti Tahir, Rahmawati Titin Agustina Trisnawati Arwien, Rizky Upyt handayani Vika Puji Cahyani Widiastini Arifuddin Widyaningsih, Arrifah Tri Wiwi Salmiati Yanti Hikma Yati BT Samsuddin Yohan, Benekdita Yunus, Muh Yusran