Article Per Year (5 Year)
p-Index From 2020 - 2025
2.693
P-Index
This Author published in this journals
All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Majalah Kedokteran Bandung Research Journal of Life Science MNJ (Malang Neurology Journal) Majalah Obstetri dan Ginekologi Biomedical Journal of Indonesia Medical Laboratory Technology Journal AcTion: Aceh Nutrition Journal Journal of Issues in Midwifery Asian Journal of Health Research
Wiyasa, I Wayan Arsana
Department Of Obstetrics And Gynaecology, Faculty Of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 21 Documents
Search

 1   2   3 
The Effect of Exposure to Escherichia coli with Low Molecular Weight Hyaluronan, High Molecular Weight Hyaluronan, Hyaluronidase in the Cervix Premature Pregnant Model of Balb/C Mice Fikhy Rizky Hapsari; Suheni Ninik Hariyati; I Wayan Arsana Wiyasa; Teguh Wiyono; Dewi Santosaningsih
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.56

Abstract

Introduction: Infection is the biggest cause of preterm labor, about 25-40%, and ascending infection through the vagina is the most common route of infection. This study aims to evaluate the level of Low Molecular Weight Hyaluronan (LMWHA), level of High Molecular Weight Hyaluronan (HMWHA), and the level of the enzyme hyaluronidase in Balb/c premature labor due to infection with Escherichia coli model mice. Methods: This study used an actual experimental design carried out in the laboratory in vivo on balb/c mice exposure to Escherichia coli with a randomized post-test only with a control group design. In the premature model of exposure to Escherichia coli balb/c mice on the cervix of 109 CFU/ml, cervical samples were taken and the levels of LMWHA, HMWHA, and hyaluronidase were measured using spectrophotometry. This study was divided into 2 groups. Control group and treatment group. Results: Low Molecular Weight Hyaluronan (LMWHA) and of the hyaluronidase enzyme levels in premature Balb/C cervical mice exposed to E. coli were higher than in Balb/C cervical mice. High Molecular Weight Hyaluronan (HMWHA) levels in premature Balb/C cervical mice exposed to E. coli were lower Conclusion: In the cervix of the infected by E. coli mice model, LMWHA and hyaluronidase enzymes increased and HMWHA decreased.    
DEALING WITH UNCONTROLLED SEIZURE IN CATAMENIAL EPILEPSY: A CASE REPORT Machlusil Husna; I Wayan Arsana Wiyasa; Ria Damayanti; Syah Sembung Wasiso; Fahimma Fahimma; Kartika Agustina
MNJ (Malang Neurology Journal) Vol. 9 No. 1 (2023): January
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2023.009.01.16

Abstract

Catamenial epilepsy refers to a seizure pattern that coincides with the menstrual period. Patients with catamenial epilepsy often leads to intractable epilepsy and may have an adverse impact on quality of life. Several medications are the therapeutic options, however, there is uncertainty regarding the best treatment, and these medications often fail to control the seizure. This makes catamenial epilepsy categorized as pharmaco-resistant epilepsy. This paper reported a case of perimenstrual catamenial epilepsy with an uncontrolled seizure problem. Seizures still occur with optimal management, and thereby clinicians must continue to re-evaluate clinical conditions and treatment selection to achieve optimal management. Personalized-based treatment should be considered in catamenial epilepsy management. This report discusses the challenges of catamenial epilepsy, understanding the catamenial process, and dealing with the problem with a practical personalized approach.
Comparison of the Effects of Dienogest and Leuprolide Acetat on Serum Interferon (IFN-Æ”) Levels in a Mouse Model of Endometriosis (Mus musculus) Maria Antonia Barbara Batu Mali; Siskanita Nur Fitriana; Sutrisno Sutrisno; Husnul Khotimah; I Wayan Arsana Wiyasa; I Wayan Agung Indrawan; Maharani Maharani
Asian Journal of Health Research Vol. 2 No. 2 (2023): Volume 2 No 2 (August) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i2.111

Abstract

Introduction: Endometriosis is a medical condition in which there is abnormal growth of tissue resembling the endometrium outside the uterus. It can cause symptoms of pain and/or infertility. The current medical therapy focuses on reducing estradiol levels or stimulating progesterone response. One of the most effective options for medical treatment is the use of GnRH analogs. Dienogest have a direct anti-inflammatory effect on endometriotic stromal cells. However, a comparison of the anti-inflammatory effects of these drugs on IFN-Æ” has not been previously investigated. Therefore, this study aims to compare the effects of reducing IFN-Æ” by both Dienogest and Leuprolide Acetat in a mouse model. Material and Methods: This study employed a post-test only control group design and involved 4 groups, namely the negative control group, positive control group, and two treatment groups: one group administered Dienogest at a dosage of 0.0052 milligrams per day for 14 days, and the other administered Leuprolide Acetat at a dosage of 0.00975 milligrams once every 5 days for a period of 14 days. Serum IFN-Æ” levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA). The data were subsequently analyzed using IBM SPSS 25 with One-Way ANOVA test. Results: This study demonstrated that Leuprolide Acetat significantly decreased serum levels of IFN-Æ”, whereas Dienogest actually increased the levels . Conclusion: In a mouse model of endometriosis (Mus musculus), Leuprolide Acetat effectively reduced the levels of IFN-Æ” (pro-inflammatory). Conversely, Dienogest increased the levels of IFN-Æ” (anti-inflammatory) in the mouse model of endometriosis.
Effectiveness of Rosmarinus officinalis and Centella asiatica Nanoemulsions Against Caspase 3 Gestational Diabetes Mellitus Expression Sutini Lamadi; Fajar Dwi Prastiwi; Tri Yudani Mardining Raras; Husnul Khotimah; I Wayan Arsana Wiyasa
Medical Laboratory Technology Journal Vol. 9 No. 2 (2023): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i2.537

Abstract

Gestational diabetes mellitus (GDM) is closely related to oxidative stress conditions in insulin resistance conditions that increase Reactive Oxygen Species (ROS) against the body's defense antioxidant mechanism. Chronic complications due to hyperglycemia in patients with GDM increase BAX / BCL2 levels which then activates the change of procaspase 3 to caspase 3, an activator of apoptosis. This study aims to determine the effect of the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions on caspase 3 expressions in zebrafish models of gestational diabetes mellitus, with a posttest-only controlled group design. The samples were divided into five groups, namely K- (EM), K+(EM+3% Glucose), P1, P2 and P3 (3% Glucose + Combination of Rosmarinus officinalis and Centella asiatica 2.5μg/ml, 5μg/ml and 10μg/ml), which will be tested for PEPCK and Caspase expression at the age of 3dpf using Real Time-PCR. The results of the correlation test of the group with caspase 3 obtained (p = 0.045) negative direction. These results show that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsion decreased Caspase 3 expression in zebrafish models of gestational diabetes mellitus. The combination of Rosmarinus officinalis and Centella asiatica nanoemulsion has the potential to reduce blood glucose levels and reduce the risk of apoptosis in gestational diabetes mellitus patients.
The Influence of Vitamin D3 Supplementation on LH and FSH Levels in Female Rats (Rattus norvegicus) with Polycystic Ovary Syndrome (PCOS) Model Gandhari, Anak Agung Sagung Agung Ayuska; Wiyasa, I Wayan Arsana; Hariyati, Suheni Ninik; Baihaqi, Irfani
Asian Journal of Health Research Vol. 2 No. 3 (2023): Volume 2 No 3 (December) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i3.133

Abstract

Introduction: Polycystic Ovary Syndrome (PCOS) is a common hormonal disorder in reproductive-aged women, characterized by hormonal imbalances such as Luteinizing Hormone (LH) and Follicle Stimulating Hormone (FSH). This study investigates the impact of Vitamin D3 supplementation on LH and FSH levels in female rats with a PCOS model. Material and Methods: In this laboratory experiment, female rats were induced with testosterone propionate for 28 days. They were divided into three groups: negative control, positive control (testosterone only), and treatment (testosterone + Vitamin D3 supplementation). LH and FSH levels were measured using the enzyme-linked immunosorbent assay method. Results: The results revealed that Vitamin D supplementation significantly reduced FSH levels in the treatment group compared to the positive control, while it increased FSH levels. Regression analysis demonstrated a negative correlation between Vitamin D supplementation and LH levels, as well as a positive correlation with FSH levels. Conclusion: Vitamin D3 supplementation appears to have a regulatory effect on LH and FSH levels in female rats with PCOS. Future research could explore the impact of higher Vitamin D3 doses and conduct further investigations at advanced stages of PCOS. These findings contribute to our understanding of PCOS and potential interventions involving Vitamin D3.
Rosmarinus officinalis and Centella asiatica Affect Interleukin-6 Zebrafish Larvae Induced High Glucose Prastiwi, Fajar Dwi; Lamadi, Sutini; Khotimah, Husnul; Wiyasa, I Wayan Arsana; Raras, Tri Yudani M.
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.538

Abstract

Gestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose.
Effect of Curcumin on Nitric Oxide and Endothelin-1 Levels in L-NAME-Induced Preeclamptic Wistar Rat Rahardjo, Bambang; Dewi, Ratih Frastika; Wiyasa, I Wayan Arsana; Handayani, Puspita; Prasetyorini, Nugrahanti; Wibisono, Hermawan
Majalah Kedokteran Bandung Vol 56, No 4 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3700

Abstract

Preeclampsia is a pregnancy disorder marked by the onset of hypertension after the 20th week, posing risks such as cardiovascular disease. Curcumin, a commonly consumed herbal medicine, has been investigated as a potential antihypertensive agent in mouse models of preeclampsia. This study took place in the Bioscience Laboratory of Universitas Brawijaya from March to August 2023, employing a true experimental design with various groups of mice receiving different treatments. Nitric oxide (NO) and endothelin-1 (ET-1) levels were measured using calorimetry and ELISA. The rats were divided into five groups: positive control, negative control and P1, P2, and P3 as the treatment groups. Treatment grups received fifferent curcumin doses of 30mg/kgBW/day, 50mg/kgBW/day, and 100mg/kgBW/day for P1, P2, and P3, respectively. Data analysis using the One-Way ANOVA and Post Hoc LSD revealed that curcumin at 100mg/kgBW/day significantly increased the NO level of 47.75±22.6 and decreased the ET-1 level of 67.03±24.47 when compared to the positive control (p<0.05). However, the 30mg/kgBW/day and 50mg/kgBW/day doses did not significantly affect the NO and ET levels. In conclusion, curcumin supplementation shows positive effects on NO and ET-1 levels in L-NAME-induced preeclamptic Wistar rats, highlighting its potential as an effective intervention for managing this pregnancy-related disorder.
The Effect of Red Bean Extract (Phaseoulus Vulgaris L. Sp.) on T Helper 17, T Regulator Levels, and Atretic Follicle Count in Mice Models of Premature Ovarian Failure Induced by Systemic Lupus Erythematosus Effendi, Madina Paramitha; Noryanto, Mukhamad; Wiyasa, I Wayan Arsana; Arianto, Onni Dwi
Asian Journal of Health Research Vol. 4 No. 1 (2025): Volume 4 No 1 (April) 2025
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v4i1.222

Abstract

Introduction: Systemic Lupus Erythematosus (SLE) is a long-term autoimmune disease that primarily impacts women of reproductive age. A major complication of SLE treatment with cyclophosphamide is premature ovarian failure (POF), leading to infertility. The imbalance between T-helper 17 (Th-17) and T-regulator (Treg) cells contributes to SLE progression. Red bean extract (Phaseolus vulgaris L. sp.), known for its antioxidant and anti-inflammatory effects, may help restore this balance and protect ovarian function. Material and Methods: Female Balb/c mice were divided into five groups: control, untreated SLE, and three treatment groups receiving red bean extract at 50, 75, and 100 mg/kg. SLE was induced using pristane, followed by cyclophosphamide to trigger POF. The treatment groups received red bean extract for four weeks. Th-17 and Treg levels were measured using ELISA, and ovarian tissues were histologically analyzed to assess atretic follicle count. Data were statistically analyzed using ANOVA and Tukey’s post hoc test. Results: Red bean extract reduced Th-17 levels and increased Treg levels, indicating improved immune balance. Additionally, a decrease in atretic follicles suggested protective effects on ovarian function. The most significant results were observed at the 75 mg/kg dose. The extract’s polyphenolic and phytoestrogenic content likely contributed to its anti-inflammatory and antioxidant activity. Conclusion: Red bean extract shows promise as a natural therapeutic agent for preventing ovarian dysfunction in SLE-induced POF by modulating immune response and reducing follicular damage. Further research is needed to explore its molecular mechanisms and clinical potential in humans.
Efficacy and role of vitamin D (25-OH-D serum) in the treatment of endometriosis: A systematic review Diani, Yuli Ayu; Wiyasa, I Wayan Arsana
Majalah Obstetri & Ginekologi Vol. 33 No. 2 (2025): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V33I22025.132-140

Abstract

HIGHLIGHTS Endometriosis frequently causes pelvic damage and infertility, and vitamin D has been proposed as a therapeutic option owing to its involvement in immune regulation and reproductive health. Vitamin D may contribute to the modulation of inflammatory pathways and decrease the risk of hyperplasia and malignancy, with several studies indicating that it might also impact infertility related to endometriosis. ABSTRACT Objective: Vitamin D, an essential element in women’s reproductive health, has shown potential benefits in endometriosis management by improving plasma concentrations and modulating immune responses in chronic inflammatory conditions, including endometriosis. This study aimed to elucidate the pivotal role of vitamin D in alleviating chronic pain and infertility associated with endometriosis and to provide new insights into its therapeutic application in reproductive medicine. Materials and Methods: This systematic review examined literature retrieved from PubMed, ScienceDirect, ResearchGate, and Google Scholar, screening 1,156 records and including 20 studies for qualitative assessment. The review focused on the therapeutic contribution of vitamin D in endometriosis management. Eligible studies were those published in the last decade, in English, involving non-pregnant, healthy women, particularly patients with chronic pelvic pain or infertility. Accepted study designs comprised experimental, cohort, longitudinal, case reports, pilot, and observational studies. Excluded were non-English articles, animal or pregnant populations, reviews, inaccessible full texts, and studies lacking relevance to vitamin D or endometriosis. Results: Twenty selected studies were analyzed, encompassing 5,346 women and 60 girls aged 12–50 years, with or without endometriosis, from Iran, the USA, South Korea, Russia, Turkey, Brazil, Poland, Italy, Germany, and Japan. These studies indicated that multiple therapeutic approaches were attempted, suggesting that endometriosis may be partially ameliorated by vitamin D administration. Conclusion: This systematic review highlights that women with endometriosis presented with lower vitamin D levels; however, the evidence regarding supplementation efficacy remains uncertain. Further large-scale, rigorously designed trials are necessary to establish vitamin D’s therapeutic role in the management of endometriosis.
Effect of Curcumin Nanoemulsion on Brain Cell Development, Locomotor Function, and Mortality of Zebrafish Larvae Model of Gestational Diabetes Mellitus Subandi, Subandi; Azahra, Mutiara Syifa; Ovinery, Amarsya; Akhmalia, Qorry' Afyu; Asih, Aliyah Tresna; Sulistomo, Hikmawan Wahyu; Nurdiana, Nurdiana; Wiyasa, I Wayan Arsana
Biomedical Journal of Indonesia Vol. 11 No. 3 (2025): Vol 11, No 3, 2025
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v11i3.270

Abstract

Introduction. Gestational diabetes mellitus (GDM) is glucose intolerance in pregnancy due to reduced ability of pancreatic beta cells to produce insulin, causing oxidative stress that triggers various complications such as brain apoptosis to locomotor disorders and decreased head size which has an impact on mortality rates. Pharmacological treatment of GDM has side effects that risk affecting fetal development. Therefore, curcumin as a herbal medicine can be an option for GDM treatment because it can increase insulin sensitivity by activating the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) receptor. Methods. Post Test Only Control Group Design was used in this experimental study which was tested on each research variable consisting of 25 zebrafish embryos divided into 5 treatment groups consisting of a negative control, a positive control exposed to 3% glucose, and a diabetic group given curcumin nanoemulsion with three different doses (0.3125 μg/ml, 0.625 μg/ml, 1.25 μg/ml). One Way ANOVA and Post Hoc Tukey tests were used to analyze the data from this study. Results. P value from One Way ANOVA test p=0.024 for brain apoptosis, p=0.00 for locomotor ability, p=0.04 for head size, and p=0.006 for mortality rate. Turkey Post Hoc test showed significant differences in the control and 3% glucose + 0.625 µg/ml curcumin nanoemulsion groups (p<0.05). Conclusion. Curcumin nanoemulsion therapy has an effect on decreasing brain apoptosis, increasing locomotor, inhibiting the decrease in head size, and decreasing the mortality rate in zebrafish with gestational diabetes. The most effective dose of curcumin nanoemulsion is 0.625 µg/ml.
Co-Authors Achmad Hidayat Adriana, Kusuma Agus Hendra Al Rahmad Akhmalia, Qorry' Afyu Annissa Febriani Arianto, Onni Dwi Arifiandi, Maya Devi Asih, Aliyah Tresna Azahra, Mutiara Syifa Baihaqi, Irfani Bambang Rahardjo DAMAYANTI, ZUHRIA PUSPITA Darwitri, Darwitri Dewi Santosaningsih Dewi, Ratih Frastika Diani, Yuli Ayu Donny Wisnu Wardhana Dwi Yuni Nur Hidayati Edy Mustofa Een Nuraenah Effendi, Madina Paramitha Elisa Danik Kurniawati Evi Zahara Eviana Norahmawati Fahimma Fahimma Fajar Dwi Prastiwi Fikhy Rizky Hapsari Gandhari, Anak Agung Sagung Agung Ayuska Hariyati, Suheni Ninik Heni Pujiastuti Husnul Khotimah Husnul Khotimah Husnul Khotimah I Wayan Agung Indrawan I Wayang Agung Indrawan Irfani Baihaqi Kalsumy, Umi Kartika Agustina Kusuma Adriana Lamadi, Sutini Machlusil Husna Maharani Maharani Maria Antonia Barbara Batu Mali Mohammad Muljohadi Ali Nany Nita Noviana Ni Putu Sri Haryati Nina Rini Suprobo Nirwan Nugraha Noryanto, Mukhamad Nugrahanti Prasetyorini Nurdiana Nurdiana Nurlaili Ramli Ovinery, Amarsya Prastiwi, Fajar Dwi Puspita Handayani Qonita Prasta Agustia Raras, Tri Yudani M. Siskanita Nur Fitriana Sri Andarini Sri Winarsih Subandi Subandi Suheni Ninik Hariyati Sulistomo, Hikmawan Wahyu Sumarno Reto Prawiro Suseno, Daniel Alexander Sutini Lamadi Sutrisno Sutrisno Sutrisno Sutrisno Suwana, Febie Putra Syah Sembung Wasiso Tanti Tri Lestary Tatit Nurseta Teguh Wiyono Tri Yudani Mardining Raras Tri Yuliyani Wibisono, Hermawan Wino Vrieda Vierlia Yahya Irwanto