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All Journal Jurnal Sumberdaya Hayati Science and Technology Indonesia Jurnal Kreativitas PKM Jurnal Ilmiah RESPATI Best Journal (Biology Education, Sains and Technology) Jurnal Integrasi Kesehatan dan Sains Jurnal Pijar MIPA Jurnal Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Zahra, Aliya Azkia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Abstract

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.
Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.) : Antibacterial and Antioxidant Properties of Bioactive Compounds Derived from Endophytic Bacteria Isolated from Nyatoh (Palaquium amboinense B.) Putra, Rayhan Helmyana; Retnowati, Dwi; Cordova, Datu Muhammad; Zahra, Aliya Azkia; Primahana, Gian; Dewi, Rizna Triana; Filailla, Euis; Sukirno; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.25-32

Abstract

Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.
DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Abstract

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.
Review Formulasi Inhaler Kokristal Antibiotik Untuk Penghantaran Terarah Pada Infeksi Saluran Pernapasan Izzati, Maulia; Zahra, Aliya Azkia; Nur, Nabiilah Zahira; Simarmata, Moses Apriyadi Sthepenson; Muini, Chantiara FM
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.12962

Abstract

Infeksi saluran pernapasan masih menjadi penyebab utama morbiditas dan mortalitas di seluruh dunia, terutama akibat meningkatnya kasus resistansi antibiotik. Sistem penghantaran obat berbasis inhalasi, khususnya dry powder inhaler (DPI), menjadi pendekatan penting karena mampu memberikan konsentrasi obat tinggi secara lokal dengan efek sistemik minimal. Review ini bertujuan untuk merangkum perkembangan formulasi inhaler kokristal antibiotik dalam meningkatkan stabilitas, efisiensi aerosol, dan efektivitas klinis. Kajian dilakukan terhadap berbagai penelitian yang melibatkan ciprofloxacin, tobramycin, rifampicin, colistin, dan azithromycin menggunakan metode spray drying, co-spray drying, serta spray freeze drying. Hasil telaah menunjukkan bahwa pembentukan kokristal mampu meningkatkan kelarutan, menurunkan higroskopisitas, dan mempertahankan fine particle fraction (FPF) di atas 60%. Ciprofloxacin menunjukkan keseimbangan terbaik antara kelarutan dan kestabilan, sedangkan tobramycin memberikan deposisi paru tertinggi dengan FPF mencapai 83%. Metode co-spray drying dan QbD-based spray drying menghasilkan partikel respirabel berukuran 1–5 µm dengan kestabilan jangka panjang tanpa kehilangan aktivitas antibakteri. Tren penelitian masa depan diarahkan pada integrasi teknologi Quality by Design, rekayasa nano-in-micro, dan penggunaan eksipien ramah lingkungan. Secara keseluruhan, strategi formulasi kokristal DPI memberikan potensi besar untuk meningkatkan terapi inhalasi antibiotik yang efektif, stabil, dan aman bagi pengobatan infeksi saluran pernapasan kronik
Co-Authors Abbas, Zuyyinna Alya Ahsanal Kasasiah Aini, Kania Nurul Anggraini, Shafira Intan Annajla, Fathina Cordova, Datu Muhammad Damara, Dandy Satria Daniel Joko Wahyono Dwi Retnowati Filailla, Euis Hermosaningtyas, Anastasia Aliesa Indah Laily Hilmi Izzati, Maulia Jekmal Malau Lestari, Agatha Nabilla Maulida, Verin Sakinah Meilani, Nanda Diva Muhareva Raekiansyah Muini, Chantiara FM Nur, Nabiilah Zahira Prastya, Muhammad Eka Primahana, Gian Putra, Hasan Etanov Putra, Rayhan Helmyana Rafifa, Muthia Rahmasari, Ratika Ratnasari, Devi Rizna Triana Dewi Sabrina, Lina Maisa Saryono Septi, Annisa Frastica Septiani, Dia Sholi, Mally Ghinan Sholih, Mally Ghinan Simarmata, Moses Apriyadi Sthepenson Siti Rohmah SUKIRNO Sukma Wibowo, Sepvia Putri Zahro, Aurora Fatimatuz Zulqifli, Iqbal