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All Journal Jurnal Kesehatan Tambusai Integrated Science Education Journal Innovative: Journal Of Social Science Research Detector: Jurnal Inovasi Riset Ilmu Kesehatan PPSDP International Journal of Education Mutiara: Jurnal Penelitian dan Karya Ilmiah Nian Tana Sikka: Jurnal Ilmiah Mahasiswa Jurnal Ventilator: Jurnal Riset Ilmu Kesehatan dan Keperawatan Medic Nutricia : Jurnal Ilmu Kesehatan Intellektika: Jurnal Ilmiah Mahasiswa Inovasi Kesehatan Global Mikroba: Jurnal Ilmu Tanaman, Sains dan Teknologi Pertanian Pentagon: Jurnal Matematika dan Ilmu Pengetahuan Alam Polygon: Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa Menulis: Jurnal Penelitian Nusantara Jurnal Pendidikan Kimia, Fisika dan Biologi Jurnal Cakrawala Pendidikan dan Biologi (jucapenbi) Konstanta : Jurnal Matematika dan Ilmu Pengelatuan Alam
Ardi Mustakim
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Analisis Morfologi Koloni dan Pewarnaan Gram Jamur Isolat Singkong (Manihot Esculenta) Novita Maharani; Ardi Mustakim
Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 3 No. 5 (2025): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa
Publisher : Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/algoritma.v3i5.759

Abstract

Cassava (Manihot esculenta) is a staple food that is widely consumed by the Indonesian population, especially in rural areas. However, during post-harvest handling and storage, cassava is highly susceptible to contamination by microorganisms, particularly fungi. These fungi can cause damage to the food, reduce its nutritional quality, and even produce mycotoxins that are harmful to human health. The increased risk of contamination has become an important concern in efforts to improve the quality and safety of cassava food products. This study aimed to identify the morphology of fungal colonies and the characteristics of fungal cell walls isolated from cassava stored in open environmental conditions for several days. The methods used in this study included isolation using Potato Dextrose Agar (PDA) media, macroscopic observation of fungal colony morphology, including color, edge, elevation, and texture, as well as Gram staining to observe the fungal cell wall properties. The results of this study revealed a diversity of fungal colony morphology, with three dominant types suspected to belong to the genera Aspergillus, Penicillium, and Rhizopus. Gram staining showed that the three isolates were characterized as Gram-negative, indicated by the absorption of safranin as the secondary stain. This study provides an initial overview of the types of fungi that may develop on cassava during storage, and it offers a foundation for further studies on the toxicity and food microbiology applications related to cassava. Morphological identification and Gram staining play an essential role in the initial screening before molecular identification is performed.
Isolasi dan Identifikasi Mikroorganisme dari Tempoyak: Produk Fermentasi Tradisional dari Jambi Setiana Safitri; Ardi Mustakim
Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 3 No. 5 (2025): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa
Publisher : Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/algoritma.v3i5.760

Abstract

Tempoyak is a traditional fermented paste made from durian in Indonesia, favored by the Malay ethnic group. While tempoyak holds a unique and significant place in Indonesia's rich culinary diversity, there is a notable gap in research about it compared to other fermented foods such as kimchi. More investigation is essential to grasp the microbial composition, interactions, and possible health advantages of tempoyak, which may offer benefits like boosting the immune system, reducing cholesterol levels, exhibiting probiotic qualities, preservation, and possessing antibacterial effects. Researchers could also discover vital insights regarding the history, cultural significance, production methods, microbiological issues, nutritional aspects, and future prospects of local foods like tempoyak. This research intends to examine and study tempoyak as a traditional fermented food through comprehensive literature review and data from earlier investigations. Tempoyak has a strong connection to Malay cultural identity, regardless of its production location. As a key element of the daily practices of the Malay people in Indonesia, tempoyak is typically featured at cultural events, weddings, or large gatherings. This cultural importance sets Indonesian tempoyak apart from its Malaysian counterpart. The process of making tempoyak involves mashing the durian flesh, mixing in salt, and letting the combined mixture ferment in a sealed container at room temperature for 4 to 7 days. The microbial population in tempoyak can differ but mainly includes Lactobacillus species, particularly Lactobacillus plantarum. Proximate analysis is an effective approach to evaluate the nutritional makeup of tempoyak. Differences in nutrient levels may arise from the various types of durian, salt elements, sterilization periods, temperatures, and length of fermentation. Potential research opportunities could explore tempoyak’s role in fostering sustainable food practices, additional health benefits from its consumption, and the development of innovative products based on tempoyak. The aim of this study will enhance the field and aid in safeguarding and promoting the culinary heritage of the Malay community.
Potensi Biokimia Aloe vera (L.) Burm.f. sebagai Agen Penyembuhan Luka dan Basis Formulasi Sediaan Topikal Farmasi Salsabila Rahman; Ardi Mustakim
Jurnal Ventilator Vol. 3 No. 4 (2025): Jurnal Ventilator
Publisher : Stikes Kesdam IV/Diponegoro Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59680/ventilator.v3i4.2137

Abstract

Aloe vera (L.) Burm.f. is a bioactive plant containing polysaccharides, anthraquinones, sterols, vitamins, minerals, and glycoproteins, contributing to wound healing through anti-inflammatory, antibacterial, antioxidant mechanisms, fibroblast stimulation, and accelerated re-epithelialization. This study employed a systematic literature review (SLR) of 37 peer-reviewed articles (2018–2025) to evaluate the biochemical potential of Aloe vera and its application as a basis for modern pharmaceutical topical formulations. Results revealed that acemannan increased fibroblast proliferation by up to 200, modulated TGF-1, enhanced type I and III collagen deposition, and accelerated re-epithelialization. Anthraquinones such as aloin and emodin exhibited antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Nanoemulsion and nanogel formulations improved transdermal penetration up to threefold compared to conventional gels. These findings highlight Aloe vera’s potential as a wound healing agent and a basis for modern pharmaceutical topical preparations.
Karakterisasi Metabolit Bioaktif Pare (Momordica charantia) sebagai Pengontrol Gula Darah dan Pendukung Pencernaan Lisa Fitriana; Ardi Mustakim
Detector: Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 1 (2026): Jurnal Inovasi Riset Ilmu Kesehatan
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/detector.v4i1.5831

Abstract

Bitter melon (Momordica charantia) is a medicinal plant traditionally used to control blood glucose levels and improve digestive health. These benefits are closely associated with its bioactive metabolite content. This article aims to characterize the bioactive metabolites of bitter melon and to review their potential bioactivity in glycemic control and digestive function. The method employed includes a literature-based characterization of metabolites, identification of major classes of active compounds, and evaluation of analytical techniques commonly applied in natural product research, such as phytochemical screening, chromatography, and spectroscopic analysis. The results indicate that bitter melon contains various bioactive metabolites, including flavonoids, alkaloids, saponins, triterpenoids, and polyphenols, which contribute to blood glucose reduction by enhancing insulin sensitivity and inhibiting glucose absorption. Furthermore, these compounds support digestive health by improving digestive enzyme activity, exhibiting anti-inflammatory effects, and maintaining gut microbial balance. In conclusion, bitter melon represents a promising source of natural bioactive compounds with significant potential for application in health and pharmaceutical development.
EKSPLORASI SENYAWA BIOKIMIA MAKANAN TRADISIONAL BERBASIS KEARIFAN LOKAL SUKU ANAK DALAM DI KABUPATEN BUNGO Yulianingtyas, Mutia Queenara; Nasanda, Shafitri, Fika; Putri, Dina Oktalia; Arifa, Rifka Fadhlina; Mustakim, Ardi
Jurnal Kesehatan Tambusai Vol. 7 No. 1 (2026): MARET 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v7i1.54104

Abstract

Pengetahuan pangan tradisional Suku Anak Dalam (SAD) di Kabupaten Bungo berperan penting dalam menjaga keberlanjutan gizi, pengobatan alami, dan identitas budaya. Berbagai pangan lokal seperti asam kemang, gulai terjun, gulai umbut, ketolo, tapai ubi, jerangau, ikan sale, madu sialang, rusip, labu kuning, keladi, tempoyak, bekasam, tebu hitam, dan asam rebung diketahui mengandung senyawa bioaktif yang memiliki potensi farmasetik. Penelitian ini bertujuan mengidentifikasi kandungan biokimia dari makanan tradisional tersebut serta meninjau potensi pemanfaatannya dalam bidang farmasi. Metode yang digunakan berupa eksplorasi... Hasil kajian menunjukkan bahwa pangan fermentasi seperti tempoyak, rusip, dan bekasam kaya bakteri asam laktat dan peptida bioaktif yang berpotensi sebagai probiotik dan antimikroba. Bahan alami seperti jerangau, asam kemang, dan madu sialang mengandung fenolik dan flavonoid yang memiliki aktivitas antioksidan dan antiradang. Umbi lokal seperti keladi dan labu kuning juga memiliki potensi sebagai pangan fungsional antidiabetes dan antioksidan. Temuan ini menegaskan bahwa pangan lokal SAD merupakan sumber berharga untuk pengembangan etnofarmasi dan pangan fungsional berbasis kearifan lokal.
Local Knowledge of Traditional Medicine and Ethnic Food Duano, Kerinci and Suku Anak Dalam Jambi As a Natural Science Learning Resource Mustakim, Ardi; Hariyadi, Bambang; Hamidah, Afreni; Falani, Ilham
Integrated Science Education Journal Vol 5 No 1 (2024): January
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/isej.v5i1.800

Abstract

Purpose of the study: The aim of this research is to explore the concept of science (biology, physics, and chemistry) in traditional ethnic medicine and food of the Duano, Kerinci, and Suku Anak Dalam Jambi. Methodology: This research examines the concept of science based on traditions and local knowledge as a source of science learning (biology, chemistry, and physics). Type of qualitative research with an ethnographic approach. Data was collected through observation, interviews, FGD, and documentation using Spradley model data analysis. Main Findings: The research found that the Duano Dalam tribe has local traditions and knowledge, such as menorah sunburn, marine ecology, lung, and gulai making. The Kerinci tribe has local knowledge about ethnic medicine and food, including Betemas/Tasapa and surian chili sauce. Meanwhile, the Anak Dalam (SAD) tribe has a tradition of Besale and local knowledge of Batangas and fish Pekasam food. The collection of traditions and local knowledge of each tribe has the potential to become a basis for science concepts in learning. Novelty/Originality of this study: The novelty or originality of the research entitled “Local Knowledge of Traditional Medicine and Ethnic Food of the Duano, Kerinci, and Anak Dalam Tribes as a Natural Science Learning Resource" lies in several key aspects. These aspects are 1) Local traditions and knowledge that can be used as a basis for science learning, 2) cultural and ethnographic perspectives as sources of science learning, 3) Interdisciplinary approaches, 4) Cross-Cultural Learning Potential, and 5) Conservation and Sustainable Practices.
Tinjauan Sistematis Senyawa Metabolit Bioaktif Buah Pepaya (Carica papaya L.) Karakterisasi Kimia dan Aplikasinya dalam Bidang Kesehatan Khusnul Khotimah Rijie; Ardi Mustakim
Inovasi Kesehatan Global Vol. 3 No. 1 (2026): Februari : Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v3i1.2812

Abstract

Papaya fruit (Carica papaya L.) is a tropical plant widely consumed as food and known to contain various bioactive metabolites with potential health benefits. The increasing interest in natural products as functional resources highlights the importance of reviewing the chemical characterization and health applications of papaya bioactive compounds. This article aims to systematically review the types of bioactive metabolites found in papaya fruit, the chemical characterization methods applied, and their potential applications in the health sector. This review was conducted through a literature study of relevant scientific articles, focusing on metabolite identification and biological activity evaluation. The results indicate that papaya fruit contains diverse bioactive metabolites, including flavonoids, phenolic compounds, alkaloids, saponins, terpenoids, and proteolytic enzymes. These compounds are commonly characterized using chromatographic and spectroscopic techniques. Several studies have reported that papaya bioactive metabolites exhibit biological activities such as antioxidant, antimicrobial, anti-inflammatory, and antidiabetic effects. This review suggests that papaya fruit has promising potential as a functional natural resource for health and pharmaceutical applications.
Profil Fitokimia dan Aktivitas Bioaktif Temulawak (Curcuma xanthorrhiza Roxb.) Berdasarkan Kajian Literatur Tassa Tassa; Ardi Mustakim
Inovasi Kesehatan Global Vol. 3 No. 1 (2026): Februari : Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v3i1.2813

Abstract

Temulawak (Curcuma xanthorrhiza Roxb.) is an indigenous medicinal plant of Indonesia that has been widely used in traditional medicine and shows considerable potential in the health and pharmaceutical fields. This potential is related to its diverse secondary metabolites with bioactive properties. This review article aims to examine the phytochemical profile of temulawak, the analytical methods used to identify bioactive compounds, and their reported biological activities based on literature studies. The method applied was a literature review of scientific articles discussing the chemical composition and biological activities of temulawak. The results indicate that temulawak contains major compounds such as curcuminoids, xanthorrhizol, flavonoids, phenolic compounds, and essential oils, which are commonly identified using chromatographic and spectroscopic techniques. Several studies report that temulawak exhibits antioxidant, anti-inflammatory, antibacterial, hepatoprotective, and potential anticancer activities. These findings indicate that temulawak has significant potential as a source of natural bioactive compounds. In conclusion, temulawak possesses a rich phytochemical profile and promising biological activities, supporting its potential application in the development of natural-based health and pharmaceutical products.
Pengaruh Ekstrak Daun Jarak (Jatropha curcas) terhadap Peradangan Muhammad Zahran Saputra; Ardi Mustakim
Inovasi Kesehatan Global Vol. 3 No. 1 (2026): Februari : Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v3i1.2828

Abstract

Inflammation is a biological response that occurs as a defense mechanism of the body against tissue damage, infection, or exposure to harmful stimuli. Excessive or prolonged inflammation can lead to various chronic diseases and negatively affect overall health. Natural compounds derived from medicinal plants have gained attention as alternative anti-inflammatory agents due to their relatively lower side effects compared to synthetic drugs. Jatropha curcas is a plant traditionally used in herbal medicine and is known to contain various bioactive compounds. This study aims to examine the effect of Jatropha curcas leaf extract on inflammatory responses. The research method used was an experimental laboratory approach with extract preparation through maceration techniques. The anti-inflammatory effect was evaluated based on changes in inflammatory indicators observed during the treatment process. The results showed that Jatropha curcas leaf extract demonstrated potential anti-inflammatory activity, which was indicated by a reduction in inflammatory signs. The presence of secondary metabolites such as flavonoids, tannins, and saponins is suspected to contribute to this effect. These findings suggest that Jatropha curcas leaf extract has promising potential as a natural anti-inflammatory agent. Further research is recommended to explore dosage optimization and toxicity levels for safe therapeutic use.  
Uji Aktivitas Jahe (Zingiber officinale) terhadap Metabolisme dan Pencernaan Khalim Purnomo; Ardi Mustakim
Inovasi Kesehatan Global Vol. 3 No. 1 (2026): Februari : Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v3i1.2829

Abstract

This study aims to examine the activity of ginger (Zingiber officinale) on metabolism and digestion through a laboratory experimental approach. Metabolism and the digestive system play a crucial role in maintaining physiological balance, and disturbances in these systems can affect nutrient utilization and overall health. Ginger has long been used as a traditional herbal remedy and is known to contain bioactive compounds such as gingerol, shogaol, and zingerone, which are believed to support digestive and metabolic functions. The research was conducted using ginger extract prepared through an extraction process under controlled laboratory conditions. Observations focused on changes in metabolic responses and digestive activity following the administration of ginger extract. The study employed a descriptive experimental design to provide an objective overview of ginger’s biological activity. The results indicated a gradual improvement in digestive activity and metabolic responses after treatment with ginger extract. These changes suggest that ginger extract has the potential to enhance digestive efficiency and support metabolic processes. The findings provide scientific support for the traditional use of ginger as a natural ingredient in maintaining metabolic and digestive health and may serve as a preliminary reference for further research using quantitative and clinical approaches.
Co-Authors Afifatur Rizqiah Afreni Hamidah Agistha Dwi Lestari Airin Nurfaiza Aisha Noviadianty Anattri, Lega Andini Anggun Sarttika Erinza Annisa Tuzzahra Arfina, Nabila Arifa, Rifka Fadhlina Arum Dwi Arimbi Astari, Milla Aulia Ramadhani Azzahra, Syasya Bambang Hariyadi Citra Bunga Lestari Dea Lestari Dea Miranda Saputri Dian Novita Dina Oktalia Putri Dini Anjani Evelyn Luna Anggraini Fathiya Rizki Aninda Felisha Putri Maida Fhariza Liandri Chardy Ilham Falani Indah Maisyarah Buliani Indri Artanti Intan Hapsari Isnaini Yulia Lestari Kayla Khairatifah Zalianti Keila Ramadhani Khalim Purnomo Khusnul Khotimah Rijie Leviona Dhina Safira Lilik Hevinovisa Lisa Fitriana Luciana Bunga Sari Lulu Wafiq Wafizo Marsya Marsya Marvina Marvina Maura Maharani Mayla Fasha Azla Milla Astari Muhammad Zahran Saputra Mulyadi, Elvaro Islami Muthiya Amalia Nailla Indayani Sumardi Nanik Apriyanti Nasanda, Fika Nasanda, Shafitri, Fika Nawra Nurvidilla Nayla Azzahrah Nayla Fazila Nadin Nayla Fazilla Nadin Nazwa Nayla Putri Nindy Adisha Puti Hanumsari Nirmala Nirmala, Nirmala Ni’matullathifah Ni’matullathifah Ni’matullathifah Novita Maharani Nyimas Fatiyah Aini Oktalia Putri, Dina Okti Sonya Rahmadani Purnomo, Khalim Putri Rahmadani Putri, Dina Oktalia Queenara Yulianingtyas, Mutia Ratu Zara Rezky Maulidiya Rifky Rifaldo Munthe Sabnatun Nikmah Saffana Aura Balqis Salsabila Rahman Setiana Safitri Shafitri Shafitri Shafitri, Shafitri Siti Dwi Cahira Sri Nanda Suci Fabilla Suci Tri Ramadhani Tamimil Hasanah Tassa Tassa Tata Anggraini Teuku Daffa Hasian Tiara Nabila Putri Tiara Nabilah Putri Yulianingtyas, Mutia Queenara Zaskia Rahmawati Zinda Rizkia Aprilia