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All Journal SOCA: Jurnal Sosial Ekonomi Pertanian Agrimeta: Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem Jurnal Bakti Saraswati Ngayah: Majalah Aplikasi IPTEKS JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA JMM (Jurnal Masyarakat Mandiri) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika Agro Bali: Agricultural Journal Abdi Masyarakat Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Seminar Nasional Aplikasi Iptek (SINAPTEK) Agrofarm, Jurnal Agroteknologi Economic Military and Geographically Business Review KOPEMAS
Putu Eka Pasmidi Ariati
Program Studi Agroteknologi Fakultas Pertanian Dan Bisnis, Universitas Mahasaraswati Denpasar
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Molecular characterization of Rice ragged stunt virus and Rice grassy stunt virus on Rice in Gianyar, Bali, Indonesia Listihani, Listihani; Selangga, Dewa Gede Wiryangga; Yuliadhi, Ketut Ayu; Yuniti, I Gusti Ayu Diah; Ariati, Putu Eka Pasmidi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12448-57

Abstract

Rice stunt disease is one of the causes of rice harvest failure. It is caused by the infection of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) infection. Information about disease severity and the molecular characteristics of stunt viruses in Indonesia is still limited. Thus, this research aimed to determine the disease severity and the genetic diversity of rice stunt viruses in Gianyar, Bali. The research method consisted of observation of incidence and disease severity in the field and virus detection by reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for RRSV and RGSV. The observation of the disease incidence and severity were performed in seven districts in Gianyar Regency, Bali, namely Blahbatuh, Gianyar, Payangan, Sukawati, Tampaksiring, Tegallalang, and Ubud. Stunt disease was found in all observation sites. High stunt disease incidence (> 44%) was found in three districts: Ubud, Tampaksiring, and Payangan, while the low disease incidence rate of <10% was found in Blahbatuh and Gianyar Districts. The highest stunt disease severity occurred in Tampaksiring District (60.82%), while the lowest severity occurred in Gianyar District (18.84%). The IR-64 and Ciherang cultivars are vulnerable to rice stunt disease infection. The highest homology of RRSV and RRGV nucleotides was found with Vietnam isolates being >98% and >97%, respectively. The phylogenetic analysis showed that Indonesian isolates of RRSV and RGSV were clustered in the same group as Vietnam isolates.
EKSPLORASI KEJADIAN PENYAKIT BUSUK BUAH (Phytophthora palmivora) PADA TANAMAN KAKAO DI DESA CAU KECAMATAN MARGA KABUPATEN TABANAN Yohanes Berckemas; Putu Eka Pasmidi Ariati; I Putu Sujana
Agrofarm: Jurnal Agroteknologi Vol. 4 No. 1 (2025): AGROFARM (JURNAL AGROTEKNOLOGI)
Publisher : Faculty of Agriculture and Business, Universitas Mahasaraswati Denpasar , Kec. Denpasar Utara, Kota Denpasar, Bali 80233

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/agrofarm.v4i1.11787

Abstract

This study focuses on evaluating the extent of cocoa pod damage caused by pathogenic infections. Understanding the underlying causes of disease is essential as a foundation for making effective control decisions. Field observations revealed that pod rot is the most significant disease due to the considerable losses it causes in cocoa production. Pod rot in cocoa, caused by Phytophthora palmivora, typically begins with the appearance of small brown spots on the surface of the pod around two days after infection. These lesions rapidly expand and darken, eventually covering the entire pod. Within approximately 14 days, the pod turns completely black, and the internal tissues, including the seeds, become dry and mummified, serving as a major source of future infections. White mycelium appears on the infected surface and becomes denser as the disease progresses. This mycelium produces sporangia containing numerous spores. These spores are spread by rainwater, either through direct contact or splash dispersal, infecting other parts of the cocoa tree. The time needed for the pod to rot completely is around two weeks, depending on the pod’s size at the time of infection. Field data indicated that in Plot I, 35 pods (14.28%) and 16 trees (5.33%) were infected. In Plot II, 22 pods (16.54%) and 19 trees (9.05%) were infected, while in Plot III, 15 pods (23.07%) and 9 trees (9.89%) were affected by pod rot. Environmental factors : air humidity, rainfall, and farming practices were identified as key variables that influence the rate of disease development and spread.
Identifikasi Morfologi dan Molekuler Jamur yang Terdapat pada Daun Tanaman Padi (Oryza sativa L.) Widnyana, I Ketut; Pasmidi Ariati, Putu Eka; Suanda, I Wayan; Suwardike, Putu
Agro Bali : Agricultural Journal Vol 7, No 2 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i2.1703

Abstract

Padi berasosiasi dengan berbagai mikroba bersifat patogenik maupun non patogenik yang berasal dari golongan jamur. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi jamur yang terdapat pada tanaman padi (Oryza sativa L.) yang dibudidayakan di kawasan Kecamatan Melaya, Kabupaten Jembrana, Bali. Penelitian ini dilakukan pada bulan April 2023 sampai bulan September 2023. Sampel diambil pada bagian daun tanaman padi dan jamur yang terdapat di dalam jaringan daun diisolasi dengan metode pengenceran, dimurnikan dan diidentifikasi secara morfologi di Laboratorium Fakultas Pertanian dan Bisnis, Universitas Mahasaraswati, Denpasar. Identifikasi molekuler dilaksanakan di Lab Biosm Indonesia Jakarta. Hasil penelitian menemukan 4 isolat jamur yaitu isolat Black, Darkgreen, Softgreen, dan White. Hasil pembacaan urutan nukleotida pada BLAST menunjukkan bahwa isolat Black memiliki kemiripan sebesar 100% dengan jamur Curvularia pseudobrachyspora asal Thailand, isolat jamur Dark Green identik 100% dengan Aspergillus clavatus dari Malaysia. Isolat, isolat Soft Green memiliki kemiripan sebesar 99,97% dengan jamur Trichoderma reesei asal China, isolat White memiliki kemiripan sebesar 99,96% dengan jamur Schizophyllum commune asal India. Berdasarkan pohon filogeni dengan similaritas >97% maka dapat disimpulkan terdapat empat spesies jamur pada tanaman padi yaitu: Curvularia pseudobrachyspora, Aspergillus clavatus, Trichoderma reesei, dan Schizophyllum commune.
Co-Authors ADE SUPRIATNA Ade Supriatna Adrianus Nurdin Ndatu Aeko Fria Utama FR Aeko Fria Utama FR Agrippina Wiraningtyas, Agrippina Amaral, Ni Putu Anglila Anak Agung Putu Gede Bagus Arie Susandya Ananda, Komang Dean Aset, Arselinus Baiq Harly Widayanti Cokorda Javandira Cokorda Javandira Denda Riratih Dewa Ayu Puspawati Dewa Putu Oka Prasiasa Dudi Septiadi Erna Cahyaningsih Farida Hanum Farida Hanum Fransiskus Diones Helena Murni Heribertus Arut Heribertus Arut Huda, Ahmad Akromul I Dewa Nyoman Raka I G N Anom I G.N. Alit Wiswasta I Gede Agus Mahendra I Gede Agus Mahendra I Gede Agus Mahendra I Gusti Ayu Diah Yuniti I Gusti Ngurah Alit Wiswasta I Gusti Ngurah Alit Wiswasta I Gusti Ngurah Anom I Gusti Ngurah Anom I Ketut Arnawa I Ketut Suada I Ketut Widnyana I Ketut Widnyana I Made Budiasa I Made Budiasa I MADE SUKERTA I Made Suryana I Made Wahyu Wijaya I Nyoman Suta I Putu Sujana I Wayan Suanda I Wayan Sumandya I.K Sumantra Irma Rubianti KETUT AYU YULIADHI Komang Dean Ananda Komang Dean Ananda Komang Dean Ananda Lalu Llham Suhendra Leni Marlina Listihani, Listihani Luh Putu Kirana Pratiwi Luh Putu Yuni Widyastuti Lutgardis Erlina Delti Lutgardis Erlina Delti Made Astagunawan Menardo Dennis Villanueva NEVIATY PUTRI ZAMANI Ni Gst. Ag. Gde Eka Martiningsih Ni Kadek Emawati Ni Made Satya Utami Ni Putu Cempaka Dharmadewi Atmaja Ni Putu Pandawani Ni Wayan Ekayanti Normiana Himan Nursan, Muhammad Olahairullah, Olahairullah Oyan Rusdianto Edwin Ole Awa Pande Komang Suparyana Pande Komang Suparyana PRATIWI, NI PUTU EKA Putu Laksmi Yulianti Sapanca Putu Laksmi Yuliyanthi Sapanca Putu Laksmi Yuliyanthi Sapanca Putu Lasmi Yuliyanthi Sapanca Putu Lasmi Yuliyanti Sapanca Putu LY Sapanca PUTU SEKARWANGI SARASWATI Putu Sri Astuti Putu Suwardike Ramdhoani Ramdhoani Ramdhoani Rasyid Ridha Rasyid Ridha Sapanca, Putu Laksmi Yulianti Selangga, Dewa Gede Wiryangga Selviana Dismiyanti Daus Simporianus Jama Sumantra, I.K Tauladan, Anan Widana, I Wayan Wijaya, I Made Wiratama, I Gusti Ngurah Made Wiswasta, I Gusti Ngurah Alit Yohanes Berckemas Yunus Wides Bani Yusak Maryunianta