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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medali Jurnal : Media Dental Intelektual MANDALA of Health Journal of Biomedicine and Translational Research INDONESIAN JOURNAL OF PHARMACY Jurnal Kedokteran Gigi Universitas Padjadjaran Journal Of Vocational Health Studies Jurnal Pengabdian Pada Masyarakat Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Health Sains JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Mandala of Health : A Scientific Journal Medical and Health Journal Molekul: Jurnal Ilmiah Kimia
Dwi Utami Anjarwati
Department Of Microbiology, Faculty Of Medicine Universitas Jenderal Soedirman, Purwokerto, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE Anjarwati, Dwi Utami; Setiawati, Setiawati; Mujahidah, Mujahidah; Hapsari, Rebriarina; Nuryastuti, Titik
Indonesian Journal of Pharmacy Vol 25 No 2, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1014.224 KB) | DOI: 10.14499/indonesianjpharm25iss2pp76

Abstract

Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion  using MTT assay. Whereas planktonic  susceptibility testing was carried out  using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in  production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.
Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto Dwi Utami Anjarwati; Wahyu Siswandari; IDSAP Peramiarti
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1224.131 KB) | DOI: 10.14499/indonesianjpharm30iss1pp1

Abstract

Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy  (SEM)  was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of  8 Gram-positive bacteria (25%) were biofilm-producing  S. epidermidis.  One of  S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing  S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both  S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics. 
Co-Authors Abdi Rahman Nursyamsi Ade Hasan Basri Agus Fitrianto, Agus Agustina, Nenden Nursyamsi Alfiyuliani, Nesia Hani Anang Kurniawan Annisa Permata Dinda Anton Budhi Darmawan Anton Budi Darmawan, Anton Budi Ari Asnani Bagus, Zahran Uzla Attaqi Desvita Sari, Desvita Diyah Woro Dwi Lestari Eman Sutrisna Fajar Wahyu Pribadi Fanni Kusuma Djati Fareza, Muhamad Salman Gembong Satria Mahardhika Gembong Satria Mahardhika Hermin Prihartini I Dewa Sang Ayu Putu Peramiarti IDSAP Peramiarti Idsap Peramiarti Joko Setyono Joko Setyono Kesumayadi, Irfan Kurniawan, Anang Lantip Rujito Maulena, Ufik Metta Ayu Susanti Metta Ayu Susanti Meylida Ichsyani Mujahidah Mujahidah Mujahidah, Mujahidah Naufalin, Rafa Purnomo Hadi Qodri Santosa, Qodri Rahmawati Wulan Sari Rahmawati Wulansari Rani Afifah Nur Histiyani Rebriarina Hapsari Rizka Hidayati Sari, Iva Puspita Setiawati, Setiawati Thianti Sylviningrum Titik Nuryastuti Vitasari Indriani Wahyu Siswandari