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All Journal International Journal of Advances in Applied Sciences Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya International Journal of Artificial Intelligence Research Collaborative Medical Journal (CMJ) Klinikal Sains (Jurnal Analis Kesehatan) EDUKATIF : JURNAL ILMU PENDIDIKAN Health and Medical Journal Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Pengabdian Masyarakat Multidisiplin Indonesia Berdaya Jurnal teknologi Kesehatan Borneo Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Compromise Journal : Community Proffesional Jurnal Abdimas Kesehatan Karawo : Journal of Community Service (KJCS) International Journal of Health Science
Valzon, May
Universitas Abdurrab
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Journal : International Journal of Artificial Intelligence Research

 1 
A protocol for Enhanced imaging and Quantification of Cervical Cell Under Scanning electron Microscope Jusman, Yessi; Jamal, Agus; Valzon, May; Hasikin, Khairunnisa; Cheok Ng, Siew
International Journal of Artificial Intelligence Research Vol 3, No 2 (2019): December 2019
Publisher : Universitas Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2476.735 KB) | DOI: 10.29099/ijair.v3i2.98

Abstract

The application of Field Emission Scanning Electron Microscopy and Energy Dispersive X-Ray (FE-SEM/EDX) for the characterization of biological samples can produce promising results for classification purpose. The limitations of the established sample preparation technique of cervical cells for FE-SEM/EDX study that differentiate between normal and abnormal cells prompted the development of a proposed protocol for the preparation of cervical cells. The proposed protocol was conducted by a McDowell-Trump fixative prepared in 0.1M phosphate buffer without osmium tetroxide at 4°C for 2 h in the fixation process. Morphologically, the cervical cells scanned under the FE-SEM/EDX did not present blackening effects, and the structure of the cells was not broken based on the FE-SEM images. Quantitatively, the possible elemental distributions in the cells, such as carbon, nitrogen, oxygen, and sodium, are detected in samples prepared by the proposed protocol. The analysed elements were validated using the Attenuated Total Reflection and Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, by avoiding osmium tetroxide fixation, the time required for sample preparation decreased significantly. This sample preparation protocol can be used for normal and abnormal cervical cells in achieving better results in terms of morphological, detected elemental distribution, and rapid in time.
Co-Authors Agus Jamal Agusmai, Riski Ahmad Naufal, Ahmad alif maulana Amelia Elvarani Aprilianti, Risa Arif Budianto Arzi Putra Persada Asiska Permata Dewi Asma Ul Husna Aulia Izzati, Aulia Ayu Permata ayuningtiyas, ratih Bagus Pramono, Kurnia Cheok Ng, Siew Deinike Marwan Deinike W Marwan Dewi Sartika Siagian Dheana Salsabiela Eddya Dinda Lestari Dona Liazarti Dona liazarti Donaliazarti Else Chandra Else Chandra Farras, Aqil Fino Hidayat, Arya Fitrianti Hari Kusryanto Hasikin, Khairunnisa Huda Marlina Wati Huda Marlina Wati Imron Khoiri, Muhammad Ismaningsih ismaningsih, ismaningsih Isna Wardaniati Juni Nurma Sari Jusman, Yessi Kenepri Kenepri Kusuma Wardani, Kharisma Laila Indah Fitriya Larysa Fernenda Lasiah Susanti Liazarti, Dona Madona, Putri Maratis Solehah, Nurul Marwan, Deinike Wanita Muga Hapdy Daulay Muhammad Al Fatih Muhammad mahrus zain Muhammad Nur Faisal Rizky Muhammad Yazid Mukhammad Fathkhur Rozaq Nanda Utami, Putri Nurmaliza, Nurmaliza Oktariani, Eva Pratiwi, Denia Prista Indria Nawang Wulan Rahmatika Hasanah, Atin Raja Alya Zahra Reza Rizky Notisa Rini Hariani Ratih Riski Agusmai Rizka Prasetya Putri Dewi Sabrina Hayati Saddam Muhdi Safardi, Hakim Salamun Salamun Samosir, Nova Relida Santi Widiasari Santi Widyasari Santi Widyasari Siagian, Dewi Sartika Sinaga, Grace Krisdayanti Siregar, Uli Astuti Siti Juariah Siti Muawanah Sitimuawanah Sri Wahyuni Suhaila, Tasha Haviva Sukri Sukri Ulhusna, Nissa Uly Astuti Siregar Uly Astuti Siregar Ummi Azizah Nasution Utami, Putri Nanda Wahyu Ramadhan Wati, Huda Marlina Widya Ade Putri Sinaga Widya Rizkika Islami Wiwi Sartika Zein, Renni Hidayati