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Biopendix: Jurnal Biologi, Pendidikan dan Terapan
Published by Universitas Pattimura
ISSN : 24074969     EISSN : 26848341     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Jurnal Biopendix publishes peer-reviewed research of special importance and broad interest in any area of biology education by research and or result of conceptual studies.
Arjuna Subject : -
Articles 282 Documents
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ANALYSIS OF FLAVONOID COMPOUNDS OF GANDARIA FRUIT (Bouea macrophylla GRIFF) AT VARIOUS Elevations IN AMBON ISLAND Papilaya, Pamella Mercy; Karuwal, Ritha; Patadungan, Ayu Kristin
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page91-96

Abstract

Introduction: Gandaria (Bouea macrophylla Griff) is a tropical fruit plant which in the Maluku region is only found in several areas, namely Ambon Island as the largest production center. Gandaria contains secondary metabolites, one of which is flavonoids, which are generally found in various plants and are very potential to be researched and developed by Indonesian researchers because they have many good benefits for living things. Methods: This study used qualitative and quantitative research methods to test the color of the gandaria fruit samples to identify the presence of flavonoid compounds and then to find out how much flavonoids were in the gandaria fruit research samples taken from various altitudes on Ambon island. Results: The results of this study indicate that the Suli area as the area with the lowest altitude where the sample was taken contains the highest levels of flavonoids with an average of 21.498 mgQE/g, then the area of medium altitude where the sample is taken, namely Kusu-kusu has a flavonoid content with an average of 9.692 mgQE/g then in the area with the highest altitude level in sampling, namely Soya with the lowest flavonoid content with an average flavonoid content of 9.084 mgQE/g.
ANALISIS KEBERADAAN BAKTERI ENTEROBACTER PADA AIR CUCIAN IKAN LAYANG (DECAPTERUS SPP) DI PASAR MARDIKA DAN PASAR TAGALAYA AMBON Manusiwa, Gellian Gabriella; Pattipeilohy, Mery; Pelamonia, Alamanda; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page42-51

Abstract

Background: Fish sales generally do not prioritize sanitation aspects in Ambon city. This study aims to determine the presence of Enterobacter bacteria and the morphological characteristics of Enterobacter bacteria in the washing water of flying fish (Decapterus spp) at Mardika Market and Tagalaya Market Ambon. Wash water samples were taken at Mardika and Tagalaya Markets in Ambon. Methods: The analysis used the Most Probable Number (MPN) method and biochemical tests (methyl red and citrate tests). Results: The results showed that 6 samples of flying fish washing water at the mardika market and tagalaya market that had been tested were identified as Enterobacter bacteria and based on the morphological characteristics found in the washing water samples of flying fish were pink, smooth edges, wavy, curved, and also not sequential and the elevation was flat and raised. The methyl red and citrate tests were negatife and positive respectively. Conclusion: Flying fish washing water at the Mardika and Tagalaya Markets has been contaminated with Enterobacter bacteria.
ANALISIS KADAR PROTEIN DAN LEMAK BIVALVIA (Atrina vexillum) DI PERAIRAN PANTAI DESA WAAI KECAMATAN SALAHUTU KABUPATEN MALUKU TENGAH Alhamid, Jaffira Salma; Tuaputty, Hasan; Arini, Ine; Rehena, Johanis Fritzgal
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page97-101

Abstract

bivalves are a group of invertebrate organism that are often found and live in the coastal waters of waai village. This animal has special adaptations that enable it to survive in areas that experience physical and chemical in intertidal waters. This organism also has adaptations to survive against currents and waves. However, bivalves do bnot have the ability to change location quickly so they are catch. Bivalves are marine biological resource that have high diversity. Therefore, the current level of exploitation continues to increase, so that from an ecological perspective it can threaten the sustainability of bivalves populations. Protein comes from the Greek word protos which means “most important”. Protein is one of a group of macronutrients (nutrients needed in large quantities), unlike other macronutrients, for fast, protein has a more important role in biomolecular formation than energy sources (building blocks of body shape). Fat is a molecule consisting of oxygen, hydrogen, carbon and phosphorus. Fat does not easily dissolve fat, a special fat solvent such as Choloform is needed.
NEW PRIMER DESIGN of MULTIPLEX POLYMERASE CHAIN REACTION (PCR) for DETECTION of Escherichia Coli and Salmonella Enterica in FOOD SAMPLE Hidayat, Muhammad Taufiq; Prayekti, Endah; Lukiyono, Yauwan Tobing; Romdhoni, Muhammad Afwan; Sari, Devi Fitriana
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page17-25

Abstract

Background: Multiplex PCR techniques are used to detect multiple pathogens at the same time simultaneously. In order to achieve the specificity and sensitivity of detection, it is important to optimize the multiplex PCR method properly. Developing suitable primers and optimizing PCR temperature to boost particular genes from pathogens are key factors for this optimization. Methods: For the simultaneous detection of Escherichia coli and Salmonella enterica in food samples, this research aims to develop a set of primers for multiplex polymerase chain reaction (PCR). Using Primer-BLAST software, the study utilizes specific primer designs for the phoA gene (Eschericia coli) and invA gene (Salmonella enterica). DNA isolation has confirmed successful extraction from the two bacterial samples. PCR was performed under different conditions, including Singleplex and Multiplex PCR, using two annealing temperatures of 53oC and 50oC. Results: The results showed that this method can effectively amplify target genes and indicate their specificity and reliability at both temperature levels. Given these results, it has successfully conducted multiplex PCR using the built primer pairs. Both annealing temperatures of 50oC and 53oC can be used to perform multiplex PCR to detect E. coli and Salmonella enterica in one PCR reaction. Conclusion: Through this research, we have created a new set of Multiplex PCR Primers and an optimized multiplex PCR technique for the simultaneous detection of E coli and Salmonella enterica in food samples. The rapid and simultaneous screening of E. coli and S. enterica, which contributes to improved food safety measures and pathogen detection in the food industry, is promising with this optimized PCR approach.
PEMETAAN TEMATIK KESESUAIAN LAHAN BUDIDAYA RUMPUT LAUT PADA PERAIRAN TELUK KAYELI KABUPATEN BURU Sangur, Kristin; Koto, Saifuddin; Irsan, Irsan; Moningka, Vincentius F D; Bastian, Putri M A; Nuraisah, Siti; Hamid Pelupessy, Iskandar Abd
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page26-35

Abstract

Background: Seaweed is widely cultivated in coastal areas of Indonesia, one of which can be found in Kayeli Bay, Buru Regency. One of the problems faced by farmers in cultivating seaweed in this area is that there is no division of suitable areas for cultivation land in the form of thematic maps, especially using physical-chemical parameters of waters. Methods: This activity includes several stages, namely location survey, data collection or collection and preparation of a database. Measurements of physical and chemical parameters of waters are carried out directly in the field (in situ) and in the laboratory (ex situ). The measurement parameters include temperature, brightness, wave height, current speed, pH, salinity, DO, nitrate and phosphate. To create a thematic map of land suitability, the ArcGis application was used. Results: Most of the physical and chemical parameters of the waters are still suitable for seaweed cultivation, except for temperature and phosphate. The results of land suitability evaluation/analysis based on water physical-chemical parameters produce two land suitability categories, namely suitable (S2) and very suitable (S1). The category corresponds to an area of ​​110.2 ha or 29% and very corresponds to an area of ​​269.8 ha or 71%. Conclusion: The thematic map of Kayeli Bay waters, Buru Regency shows that the suitability of seaweed cultivation land is divided into two categories, namely suitable at 29% and very suitable at 71%. However, the temperature and phosphate parameters at the time of measurement were in the category not suitable for seaweed cultivation.
KOMPOSISI BOTANI HIJAUAN ALAMI DAN PRODUKSI HIJAUAN PAKAN DI AREAL PERKEBUNAN KELAPA DALAM SEBAGAI PAKAN TERNAK RUMINANSIA DI KECAMATAN TANIWEL KABUPATEN SERAM BAGIAN BARAT Eoh, Marna; Rehatta, Lea Marylin
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract Background: The need for and provision of forage is achieved by considering the continuous provision of forage in both quantity and quality. One of the efforts to develop animal husbandry that can be carried out is to utilize forage for livestock in deep coconut plantations. This research aims to determine the botanical composition of natural forage in coconut plantation areas as feed for ruminants in Taniwel District, West Seram Regency. Method: This research uses a purposive sampling method. The data used in this research is based on secondary data and primary data. Results: The research results showed that the botanical composition results were 63.18% grass, 26.30% weeds and 10.52% legumes. Production of forage in the coconut plantation area for fresh material is 16,698 tons/year and for dry material 5,159 tons/year. Conclusion: Based on the research results, it is necessary to eradicate pest plants (weeds) for forage and plant superior forage to increase the need for feed for ruminant livestock. The availability of forage, especially the production of forage, can still meet the need for animal feed in Taniwel District, West Seram Regency. Key words: Botanical composition of forage, Production of forage Abstrak Latar Belakang: Kebutuhan dan penyediaan hijauan pakan dicapai harus memikirkan penyediaan hijauan pakan yang kontinyu baik kuantitas maupun kualitasnya. Salah satu upayah pengembangan peternakan yang dapat dilakukan yaitu memanfaatkan hijauan pakan ternak diperkebunan kelapa dalam. Penelitian ini bertujuan untuk mengetahui komposisi Botani hijauan alami di areal perkebunan kelapa dalam sebagai pakan ternak ruminansia di Kecamatan Taniwel Kabupaten Seram Bagian Barat. Metode: Penelitian ini menggunakan metode purposive sampling. Data yang digunakan dalam penelitian ini berdasarkan data sekunder dan data primer. Hasil: Hasil penelitian menunjukan bahwa Hasil komposisi botani, sebesar 63,18% rumput, 26,30% gulma, dan 10,52% leguminosa. Produksi hijauan pakan ternak diareal perkebunan kelapa dalam untuk bahan segar sebesar 16.698 ton/ tahun dan untuk bahan kering 5.159 ton/ tahun. Kesimpulan: Berdasarkan hasil penelitian perlu dilakukan pemberantasan tumbuhan pengganggu (gulma) hijauan pakan dan menanam hijauan unggul untuk menambah kebutuhan pakan bagi ternak ruminanasia. Ketersediaan hijauan terutama produksi hijauan pakan masih dapat memenuhi kebutuhan pakan ternak di Kecamatan Taniwel Kabupaten Seram Bagian Barat. Kata kunci: Komposisi botani hijauan pakan ternak, Produksi hijauan pakan
KAJIAN PERILAKU MASYARAKAT PESISIR YANG MENGAKIBATKAN KERUSAKAN LINGKUNGAN SEKITAR EKOSISTEM MANGROVE DI PANTAI HAMADI KOTA JAYAPURA Tuhumena, Lolita Constantia; Umbekna, Sara; Rumahorbo, Basa T; Paranoan, Nicea Roona; Mandey, Vera K; Hehanussa, Kedswin G
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page8-16

Abstract

Background : Environmental damage around the Hamadi coastal mangrove ecosystem, South Jayapura District, Jayapura City, which is caused by many human behavioral factors on the coast, one of which is due to tourism activities that throw away garbage so that it becomes dirty and the natural beauty is damaged. he waste has not been fully addressed effectively if the management is not carried out properly, which then becomes a serious threat to the sustainability of the mangrove ecosystem area near Hamadi Beach which is used as a tourist spot. Therefore, it is necessary to conduct a study of the behavior of the community living around the area and utilizing the area. Method: The researcher used 30 respondents as samples and conducted in June to August 2024 on Hamadi Beach, South Jayapura District, Jayapura City using the data usage technique in this study is Simple Random Sampling, which is carried out randomly from members of the population without considering the level of the population. The data that has been obtained is analyzed using the Likert Scale. Results: Respondents were dominated by men, productive age 36-45 years old, predominantly high school education, fishermen, who had an income Rp 1.000.000 -Rp2.000.000. Socio-economic characteristics describe respondents who live, sell and travel around the area. The characteristics of the respondents were seen from the community's perception of the knowledge of the function of the mangrove ecosystem (3,39) including the category strongly agree, Public Perception of Environmental Conditions for the Growth of Mangrove Ecosystems (3,5) Including the category of strongly agreeing, public perception of the utilization of mangrove ecosystems (3,14) including the category of agree. Conclusion: The development of science about public perception that results in environmental damage to the mangrove ecosystem on Hamadi beach needs to be known in early countermeasures.
EFFECTS OF ADDITION BAGASSE DEGRADANT BY CELLULOLYTIC BACTERIA Bacillus subtilis ON GROWTH MEDIA OF OYSTER MUSHROOM Pleurotus ostreatus Hibatullah, Juanita; Safitri, Mutia; Harlis, Harlis; Budiarti, Retni Sulistiyoning
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page52-57

Abstract

Background: Sugarcane (Saccharum officinarum L.) serves as a valuable resource for the sugar and beverage industries, a significant quantity of its by-product, bagasse, remains underutilized. A promising strategy for increasing the value of bagasse involves improving its substrate quality through the application of cellulose-degrading bacteria, such as Bacillus subtilis. Methods: The objective of this research is to investigate the influence of bagasse degradant supplementation on the growth of oyster mushrooms. A randomized experimental design was employed to evaluate growth media containing varying concentrations of bagasse degradant (500, 600, 700, and 800 gram) in comparison to a control group. The study assessed the impact of bagasse degradant on pin diameter, fresh weight, dry weight, and water content, using ANOVA and the Duncan's Multiple Range Test (DMRT) at a significance level of 5%. Results: The study showed the addition of bagasse degradant affected the pin diameter, fresh weight, and dry weight of oyster mushroom. However, it is not effective towards the water content of oyster mushroom. Conclusion: The addition of bagasse degradant affected the pin diameter, fresh weight, and dry weight, whereas the water composition did not affected the growth of P. ostreatu
KUALITAS ORGANOLEPTIK SUSU NABATI BIJI CEMPEDAK (Artocarpus Champeden) TERSUSPENSI PROBIOTIK BERDASARKAN SUHU PENYIMPANAN Septiana, Nurul; Rohmadi, Mukhlis
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page68-75

Abstract

Background: Cempedak (Artocarpus champeden) is a local fruit of Central Kalimantan that is oval with dark yellow and fibrous flesh and has a fragrant aroma when ripe. So far, cempedak seeds have only become organic waste, not used properly. The nutritional and mineral content of cempedak seeds can be used and processed into a healthy drink, namely vegetable milk. Methods: The method used is an experimental method to produce vegetable milk with cempedak seeds which is then carried out organoleptic tests to determine the quality of the aroma and taste of the vegetable milk made. Results: Based on the research carried out, the results showed that the taste quality of vegetable milk of cempedak seeds (Artocarpus champeden) that was most liked by the respondents/panelists was at the temperature of T1=20o C and T2=30o C, which was with a refreshing sweet and sour taste, while for the sour aroma and distinctive smell of cempedak seeds at the temperature of T3=30o C. Conclusion: Cempedak seeds can be used as probiotic plant-based milk with an optimal storage temperature of 30o C to get the distinctive taste, aroma and smell of cempedak seeds.
THE EFFECT of The PROBLEM BASED LEARNING (PBL) MODEL on STUDENTS’ SCIENCE LITERACY SKILLS on INDONESIA’S ECOLOGY and BIODIVERSITY MATERIALS Fatmawati, Junita; Lestari, Tuti; Putri, Azza Nuzullah; Azhar, Aulia
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page76-82

Abstract

Background: Science literacy is the capacity to use clinical information to pick out troubles and draw conclusions based totally on proof so that you can understand and make selections approximately nature and modifications made to nature via human activities. Based on PISA data and preliminary study data, the literacy of science competencies of Indonesian students is still low. One of the learnings that has a positive influence on science literacy is problem-based learning (PBL). Methods: This study applied the Quasi Experiment method with the Nonequivalent Control Group Design. This research was conducted at SMPN 1 Gunung Talang, Solok Regency, West Sumatra in class VII in the 2023/2024 school year. Results: The average value of the experimental class is higher than the average of the control class on the posttest data. The hypothesis test results show that t'count 3.372 is greater than t'table 2.008, so H0 is rejected and H1 is accepted. Conclusion: The application of the Problem Based Learning (PBL) model affects the science literacy skills of students in Indonesian Ecology and Biodiversity material.

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