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I KETUT MUDITE ADNYANE
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INDONESIA
ACTA VETERINARIA INDONESIANA
Published by Institut Pertanian Bogor
ISSN : 23373207     EISSN : 23374373     DOI : -
Core Subject : Health,
Veterinary
Acta Veterinaria Indonesiana (Indonesian Veterinary Journal) mempublikasikan artikel-artikel dalam bentuk: penelitian, ulasan, studi kasus, dan komunikasi singkat yang berkaitan dengan berbagai aspek ilmu dalam bidang kedokteran hewan, biomedis, peternakan dan bioteknologi. Artikel ditulis dalam bahasa Indonesia atau Inggris. Acta Veterinaria Indonesiana diterbitkan oleh Fakultas Kedokteran Hewan bekerjasama dengan Perhimpunan Dokter Hewan Indonesia. Terbit dua kali dalam satu tahun pada bulan Januari dan Juli. [ISSN 2337-3202, E-ISSN 2337-4373]
Arjuna Subject : -
Articles 332 Documents
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Influence of Melatonin as an Antioxidant on the Preservation of Liquid Ram Semen in Tris-Fructose Egg Yolk Diluent Pattikawa, Vapriel Andhika; Karja, Ni Wayan Kurniani; Fahrudin, Mokhamad; Prasetyaningtyas, Wahono Esthi
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.52-59

Abstract

Excessive free radicals can induce oxidative stress, leading to the inhibition of phosphorylation processes that supply energy for sperm capacitation and the acrosome reaction in spermatozoa during fertilization. This study investigated the impact of incorporating the antioxidant melatonin into the tris-fructose egg yolk diluent on the quality of ram liquid semen preserved at 4°C. Fresh semen with ≥70% motility was diluted in the tris-fructose egg yolk group (control), tris-fructose egg yolk with 0.5 mM melatonin (TFEY + M0.5), tris-fructose egg yolk with 1 mM melatonin (TFEY + M1), tris-fructose egg yolk with 2 mM melatonin (TFEY + M2). Semen was stored in the refrigerator at 4°C for 5 days (D0−D5). The motility, viability, abnormality, and plasma membrane integrity of the semen were evaluated every 24 hours. The results indicated no significant difference (P>0.05) between the control group and the liquid semen treatment at D0. Liquid semen quality was significantly improved with melatonin treatment compared to the control group. The results of this study indicate that TKTF + M0.5 had the highest motility and viability values ​​and can maintain abnormality values. The TKTF + M2 group had a higher plasma membrane integrity value than the other treatment groups.
Effectivity and Safety of Endo-1,4 -xylanase (Xylanase), Endo-1,3(4) beta-glucanase (Beta-glucanase), Endo-1,4 beta-glucanase (Cellulase), and α Amylase as A Growth Promoter in Broiler Chickens Fadholly, Amaq; Aurellia, Sharon
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.37-43

Abstract

This study evaluates the effectivity and safety of Endo-1,4 -xylanase (Xylanase), Endo-1,3(4) beta-glucanase (Beta-glucanase), Endo-1,4 beta-glucanase (Cellulase), and α Amylase that packed in Ronozyme-AX ct (MGHSPHYT+) as an alternative to antibiotic growth promoter. A total of 64 chickens were used, divided into four groups, with each group divided into two replicates, 8 chickens per replicate. The groups were divided into treatment group, and groups administered with the different dosage of supplementation, namely 184, 230, and 276 gram/ton feed for 28 days, consecutively. Parameters such as weekly weight gain, final weight, feed conversion ratio (FCR), and carcass weight were observed to determine growth performance. Meanwhile, parameters such as mortality, blood hematology and biochemistry were observed to determine the safety of the supplementation. Based on the results, there was a significant difference on weekly weight gain, particularly in the second week, and in the final weight, with the group administered with 184 gram/ton feed showing the highest result. Meanwhile, other parameters such as FCR and carcass weight did not show any significant difference. This may be due to the enzymes not working directly to increase feed utilization but work with improving nutrient digestibility and utilization in the body. The safety parameters showed that the supplementation was relatively safe as no significant difference was observed in mortality, blood constituents and biochemistry.
Identification of Herpesviridae in Macaca Fascicularis Using the Nested PCR Method at PSSP IPB Malik, Abdul; Sulistiawati, Erni; Yuliana; Saepuloh, Uus
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.60-66

Abstract

PCR technique is used to detect the presence of Herpesviridae viruses in Macaca fascicularis that exhibit no clinical symptoms and identify them using nucleotide base sequences. This study aimed to detect and identify the presence of nucleotide base sequences in the Herpesviridae virus family in Macaca fascicularis at PSSP IPB. The study utilized 20 nasal swab samples from Macaca fascicularis. Extraction of DNA utilizing the QiaAmp™ DNA mini kit and subsequent measurement of DNA concentration. Amplification of target DNA utilizing the nested PCR technique. Sample with positive results from electrophoresis were sequenced to obtain nucleotide sequences. The average measurement value of DNA extraction concentration was 25.07 ng/µl. The DNA purity ratios at wavelengths A260/A280 and A260/A230 averaged 1.95 and 0.27, respectively. The electrophoresis results indicated a band size of 215 bp in sample code 01, corresponding to the positive control band. The sequencing results were analyzed using BLASTn on the NCBI site, revealing similarity with Macaca fascicularis lymphocryptovirus. The BLAST sample results demonstrated a Query Cover value of 100% and a percentage of identity of 98.84%.
Isolation and Molecular Identification of Nitrate-Reducing Bacteria from Swiftlet Houses in Sumedang, West Java Ningrum, Siti Gusti; Novik Nurhidayat; Titin Yulinery; Evi Triana; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.1-7

Abstract

Edible bird‘s nest is an important export commodity that is currently of concern to the Indonesian government. One of the requirements for exporting edible bird’s nest to the People's Republic of China is that this product must meet the requirements for nitrite content in the product below 30 ppm. This nitrite contamination can be obtained from the results of nitrogen metabolism from nitrifying bacteria. However, information on nitrite-reducing bacteria in swiftlet houses has never been reported and is urgent in controlling nitrifying bacteria in swiftlet houses. The presence of nitrite-reducing bacteria needs to be identified to prove the presence of these bacteria in swiftlet houses that have the potential to contribute to nitrite contamination in edible bird’s nest. This study aims to isolate nitrate-reducing bacteria in an effort to control nitrite using bacteriophages in the future. This study targeted nitrate-reducing bacteria collected from environmental samples (waste, feces, pond water, artificial pond water, soil, swiftlet eggshells, white edible bird’s nest (Aerodramus fuciphagus), black bird’s nest (Aerodramus maximus)) (n=40) from two different swiftlet houses in Sumedang, West Java, Indonesia. All isolates collected were subjected to a series of microbiological tests, phenotypic characterization (Gram staining, morphology, sugar fermentation ability, enzymes, etc.) and genotyping by PCR amplification and 16S rDNA gene sequencing. Raw sequencing data were analyzed using DNASTAR® software for DNA sequence alignment and phylogenetic tree construction. In the present work, four bacteria species were identified, including Priestia megaterium, Pseudomonas putida, Stenotrophomonas maltophilia, and Proteus terrae. To our knowledge, this study is the first report of nitrate-reducing bacteria isolated from birdhouses.
Method Modification of Developing Ischemic Stroke Animal Models Using Intravenous Catheters in Rats Perdhana, Ika Satya; Handharyani, Ekowati; Darusman, Huda Shalahuddin; Nugroho, Setyo Widi; Maheshwari, Hera; Fitri, Arni Diana
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.67-76

Abstract

In various parts of the world, including Indonesia, the incidence of ischemic stroke remains high, and its impact poses a significant burden. This condition is influenced by numerous limitations in ischemic stroke therapy. Research on ischemic stroke is therefore crucial to develop, making the creation of appropriate animal models a vital necessity. One of the most commonly used methods by researchers to create animal models is the Middle Cerebral Artery Occlusion (MCAO) method. However, in Indonesia, its application remains limited due to the lack of available facilities. Method modifications can be implemented to enable the development of animal models in Indonesia. This study employed modifications to the MCAO method on eight Sprague Dawley rats. The modification involved using an intravenous catheter to facilitate the insertion of the filament as an occluder in the middle cerebral artery of the rats. Based on clinical and pathological observations, it can be concluded that the modified MCAO method used in this study is suitable for creating ischemic stroke animal models. With this modification, the creation of ischemic stroke animal models can be carried out in a simpler and more cost-effective manner.
The Effect of Endo-1,4-β-xylanase as a Feed Additive on the Growth and Overall Health of Broiler Chickens Fadholly, Amaq; Aurellia, Sharon
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.16-21

Abstract

Enzyme has been used widely as a feed additive to complement poultry needs of nutrition. Endo-1,4 beta-xylanase as its main component, which is effective in digesting non-starch polysaccharides (NSPs), hence improving the digestive system. This research aims to observe the efficacy of Endo-1,4-β-xylanase as a growth promotor and its safety. A total of 96 chickens were used in this research, divided into four groups. Administration depending on each group was conducted for 28 days, consecutively. Growth parameters that were observed includes body weight gain and feed conversion ratio, meanwhile for safety assessment, the parameters used were mortality rate, and blood profile. Based on the results, the enzyme was proven to enhance growth performance through the increased body weight gain and final weight. This is due to the enzyme’s mechanism which improves nutrient utilization. Based on the safety assessment, Endo-1,4-β-xylanase was also proven to be safe, not causing any inflammation responses in the body nor organ damage based on the blood assessment.
The Expression of Type III Collagen Connective Tissue inGluteobiceps and Psoas Mayor Muscles of Aceh Cattle Tanjung, Fitri Aisah; Ali, Ihfan Khaidir; Jalaluddin, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Hidayat, Taufan; Wardhani, Lailia Dwi Kusuma; Ritonga, Mudhita Zikrullah; Rahmi, Erdiansyah; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Sofyan, Hamny
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

Collagen is one of the determinant factors of beef tenderness. This study analyzed the expression and content of type III collagen connective tissue in two muscle types of Aceh cattle through immunohistochemical staining. Meat samples were obtained from the gluteobiceps and psoas major muscles of three Aceh cattle bulls weighing 250-300 kg, with a body condition score of three. Histology preparations were created from samples and subjected to immunohistochemical staining utilizing specific antibodies targeting type III collagen. Qualitative data were analyzed descriptively, while quantitative data were analyzed using IMAGE J software. The results showed that the expression of type III collagen connective tissue was found in the endomysium and perimysium layers of both muscles. The expression of type III collagen connective tissue varied significantly among the muscles (P<0.05). The gluteobiceps muscle had an average percentage of the area containing type III collagen connective tissue of 4.47±2.41%, while the psoas major muscle was lower at 2.51±1.69%. This study concludes that the gluteobiceps muscle of Aceh cattle has a higher amount of type III collagen connective tissue than the psoas major muscle.
Feline Leukemia Virus (FeLV) in Cats Domestic: A Case Study Fatra, Ilhami Maulia; Cadiwirya, Pradhana Kurniawan; Prasetyo, Dodik; Noviatri, Aldila; Kristanto, Dwi
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

Feline Leukemia Virus (FeLV) is one of the major causes of death in cats. A 4-year-old domestic cat weighing 2 kg presented with decreased appetite, weakness, sneezing, and nasal discharge. Physical examination revealed gingivitis accompanied by ulcers in the mouth and pale mucous membranes. Further diagnostic tests included hematology examination, blood chemistry examination, and the FeLV test kit. The results of hematology and blood chemistry examinations reported that the animal had leukocytosis, neutrophilia, thrombocytopenia, hypochromic macrocytic anemia, hyperproteinemia, hyperglobulinemia, decreased A/G ratio, hyperamylasemia, hyperbilirubinemia, and azotemia. Rapid test (kit) examination showed a positive result for FeLV. Based on the diagnostic results, the initial diagnosis was suspected Feline leukemia virus (FeLV) with an infausta prognosis. The treatment included antibiotics Amoxicillin at 18 mg/kgBW, Diphenhydramine HCl 1 mg/kgBW, Ornipural® 0.2 ml/kgBW, Kenalog® 1 mg/kgBW, Hematovit® dose 0.4 ml/kgBW, and supplements in the form of Vitamin C 0.5 ml/day and Fufang® 1 ml/kgBW. Despite the treatment, the cat’s condition did not improve and it succumbed on the sixth day of treatment. 
Reproductive Performance of Multiparous Ewes with Different Flushing Ration Manage-ment: Reproductive Performance of Multiparous Ewes after Flushing Ration Management Triansyah, Irvan; Astuti, Dewi Apri; Arifiantini, R. Iis
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.126–132

Abstract

Local sheep are small ruminants that are kept by many farmers. The reproductive success of the animals is influenced by the ration offered. The aim of this study is to evaluate the reproductive performance of multiparous ewes with different flush management. For the study, 16 multiparous ewes were used in a 4 x 4 randomized group design. Treatments consisted of P1 = no flushing, P2 = flushing before and after mating, P3 = flushing before and after mating and at mid-pregnancy, P4 = flushing before and after mating, at mid-pregnancy and at the end of pregnancy. The variables observed were the percentage of pregnancy, number of embryos, embryo diameter, amnion diameter, embryo durability, number of offspring and duration of pregnancy. The results showed that the management of flushing rations influenced the pregnancy rate and amniotic diameter. The number and diameter of embryos, gestation length, number of offspring and embryo lifespan did not differ between treatments (P>0.05). This study concludes that the management of flushing rations affects reproductive performance in terms of pregnancy rate and amniotic diameter.
Anatomical and Histological Features in Muscles of Wild Boar (Sus scrofa) from Timor Island maha, Inggrid Trinidad; AMALO, FILPHIN ADOLFIN; NITBANI, HENY; SELAN, YULFIA NELLYMALIK; jacob, jois; odung, Elisabeth Memot
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

The anatomical features and histology of wild boars serve as two parameters for assessing the quality of wild boar meat. This study aimed to identify the anatomical and histological characteristics of wild boar muscles (Sus scrofa) from Timor Island, with potential benefits for the community and veterinary students. The research samples were the longissimus dorsi and biceps femoris muscles from three wild boars slaughtered in the laboratory. Muscle samples were prepared for anatomical observations. Muscle samples were anatomically observed, fixed in 10% alcohol, and then prepared for histological analysis using Hematoxylin and Eosin (HE) staining. The anatomical observation indicated that the boar's muscles exhibited a blackish-red coloration. The longissimus dorsi muscles had a chewy consistency, while the biceps femoris muscles were also chewy, displaying slight density and the distinctive aroma of fresh meat. Histological images indicated that muscle fibers appeared polygonal in shape in transverse sections, while elongated in longitudinal sections. Some fasciculi composed of muscle fibers exhibited multiple nuclei located at their peripheries. Between the muscle fibers, round fat cells with a nucleus were surrounded by three types of connective tissue. The histological characteristics of wild boar exhibit similarities to those of the Timor pig, Sumatran Ongole cattle, and Bali cattle.

Page 31 of 34 | Total Record : 332

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