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Contact Name
Mohammad Rizki Fadhil Pratama
Contact Email
mohammadrizkifadhilpratama@umpr.ac.id
Phone
+6287815093560
Journal Mail Official
bjop@umpr.ac.id
Editorial Address
Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya Building B 1st Floor, RTA Milono St. Km.1,5. Palangka Raya 73111, INDONESIA
Location
Kota palangkaraya,
Kalimantan tengah
INDONESIA
Borneo Journal of Pharmacy
ISSN : -     EISSN : 26214814     DOI : https://doi.org/10.33084/bjop
Core Subject : Health,
Borneo Journal of Pharmacy publishes various scientific articles covering Pharmacy and Pharmaceutical Sciences in the field but not limited to: Pharmacology-Toxicology, including pharmacokinetics, pharmacodynamics, pharmacotherapy, and toxicology. Pharmacognosy-Phytochemistry, including pharmacognosy, phytochemistry, ethnobotany, and ethnopharmacology. Pharmaceuticals, including biopharmaceuticals, pharmaceutical technology, formulations, and biotechnology. Analytical Pharmacy-Medicinal Chemistry, including pharmaceutical chemistry, chemical analysis, medicinal chemistry, bioinformatics, pharmacy physics, pharmaceutical analysis, and method validation. Microbiology Pharmacy, including the antibacterial, antifungal, and antiviral activity test. Natural Product Development, including testing the pharmacological activity of extracts, fractions, and plant isolates. Clinical-Community Pharmacy, including monitoring usage, side effects, counseling, and drug use evaluation. Management Pharmacy, including drug management, drug use profiles, pharmaceutical administration, and marketing.
Articles 311 Documents
Phytochemical Insights into Rhizophora stylosa: pH-Dependent Extraction, Antioxidant Activity, and Enzyme Inhibition Potential Pambudi, Dwi Bagus; Muhtadi, Muhtadi; Maryati, Maryati
Borneo Journal of Pharmacy Vol. 9 No. 1 (2026): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v9i1.11158

Abstract

The solvent properties, including pH, highly influence the extraction efficiency of these bioactive compounds. This study investigated the effect of pH modulation in the ethanol solvent on the recovery of antioxidants from Rhizophora stylosa. Ethanolic extraction was performed on R. stylosa samples using solvents adjusted to varying pH levels (4, 8, 9, 10, and 12). The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were quantified using standard colorimetric assays. The antioxidant activity was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The inhibitory activity against carbohydrate-digesting enzymes was assessed through α-amylase and α-glucosidase inhibition assays. Additionally, data mining of established natural product databases (KNApSAcK and IMPPAT) and literature was conducted to identify and catalog the known bioactive constituents of R. stylosa. The results demonstrated a significant correlation between the pH of the extraction solvent and the yields of phenolic compounds, flavonoids, and the resulting antioxidant activity. Extract obtained at pH 10 yielded the highest TPC and exhibited the strongest antioxidant activity, whereas the highest TFC was observed at pH 4. Furthermore, enzymatic inhibition assays revealed that the pH 10 extract displayed moderate α-amylase inhibitory activity (IC₅₀ = 76.7 µg/mL) but substantially weaker α-glucosidase inhibition (IC₅₀ = 190 µg/mL), demonstrating a selective inhibition profile though less potent than that of acarbose. These findings establish pH-modulated extraction as a targeted strategy to produce potent, phenolic-rich antioxidant extracts from R. stylosa, highlighting its potential for nutraceutical and pharmaceutical applications.

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