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Development Of Biotechnology Teaching Materials: E-Comics Complemented by Factual Videos to Improve Science Literacy Skills Wahyuni Lubis; Diky Setya Diningrat; Ashar Hasairin; Muhammad Azhari; Adi Hartono
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9303

Abstract

Biotechnology might be difficult to understand. Comics were learning media that presented the material in the form of illustrated stories to make it more interesting, fun, not boring, and easily understood by students. Innovation makes comics in digital form, namely e-comics equipped with factual videos. The purpose of this study was to determine the feasibility of biotechnology e-comics equipped with factual videos, as well as the readability and response of students to biotechnology e-comics media equipped with factual videos. This research uses the ADDIE development model starting from the analysis, design, development, implementation, and evaluation stages. The samples used in this study were randomly selected XII-grade students of Sultan Daulat 1 Senior High School. Based on the research that has been done, the results obtained are: 1) Biotechnology e-comic media complemented by factual videos obtained a material validation score of 84% and media validation obtained a score of 93% with a very valid category. 2) Comic media obtained a readability score of 81.4% with a very good category 3) Students' responses to comic media obtained a score of 82% in the very good category. Based on these results, the biotechnology e-comic media equipped with factual videos is categorized as very valid, so it is feasible to use it as a learning media.
In Silico Analytic of Antiacne Potential from Jamblang Flower’s Essential Oil Formulation Hasibuan, Nurfadilah Azizah; Diningrat, Diky Setya; Sari, Ayu Nirmala
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the components of compounds contained in Jamblang flower essential oil (Syzygium cumini L.) as antiacne and mechanism of antiacne. Jamblang flower essential oil is obtained through a distillation process. The distilled oil was then analysed using a Gas Chromatography Mass Spectrophotometer (GC-MS). Analysis results GC-MS evaluated using the program MASSLAB. Data obtained from the tool GC-MS then analysed using software PubChem NCBI database (https://pubchem.ncbi.nlm.nih.gov/) and PASS Online. The results of the GC-MS analysis showed that there were 47 bioactive compounds in Jamblang flower essential oil. Analysis results PubChem showed 13 compounds have activity as an antibacterial. Analysis results PASS Online demonstrated an antibacterial mechanism, protein synthesis inhibitor, membrane permeability inhibitor dan cell wall synthesis inhibitor, with the highest Pa value of 0.804 in the compound Benzyl benzoate $$Benzoic acid, phenylmethyl ester (CAS) and lowest Pa value in 1H-Benzimidazole,2-(methylthio)-(CAS)$$2-thiomethylbenzimidazole.
Analisis Ekstrak Etanol Tangkai Daun Buasbuas (Premna pubescens) Menggunakan Gas Chromatography Mass Spectrophotometer (GCMS) Diky Setya Diningrat; Martina Restuati; Kusdianti Kusdianti; Ayu Nirmala Sari; Erly Marwani
Elkawnie Vol. 4 No. 1 (2018)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v4i1.3075

Abstract

Buasbuas (Premna pubescens) is a plant that is traditionally known to have medicinal properties. This study aims to determine the content of bioactive compounds contained in the petiole by Gas Chromatography Mass Spectrophotometer (GCMS) method. Preparation of ethanol extract of petiole using maceration method with 96% ethanol solvent. The study used gas chromatographic tools and mass spectra which were evaluated using MASSLAB program. The data obtained from the GCMS machine is then analyzed using the NCBI database pubchem software (https://pubchem.ncbi.nlm.nih.gov/). The results of this study indicate that the content of bioactive compounds on the petioles of buasbuas more than 50 libraries contains about 150 species of compounds with a range of RT and% area respectively 4.684 to 28.155 and 0.16 to 15.56%. The content of bioactive compounds shown this data indicates that very large potential of buasbuas plants to be explored and exploitation as a nutritious plant. The results of this study can be used as the foundation in the development program of the potential utilization of bioassemblance of buasbuas plants. In further research it is necessary to analyze the other parts of the plant and make comparisons to complete the available databases.
Analisis GC-MS Senyawa Bioaktif Pencegah Penyakit Degeneratif Dari Ekstrak Etanol Kulit Buah Jamblang (Syzygium Cumini) Ayu Nirmala Sari; Kusdianti Kusdianti; Diky Setya Diningrat
Elkawnie Vol. 4 No. 2 (2018)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v4i2.4143

Abstract

Penelitian ini bertujuan untuk membuat inventarisasi database antioksidan alami dalam level molekuler dari tanaman jamblang (Syzygium cumini) dengan pendekatan Gas Chromatography Mass Spectrometer (GCMS) sehingga dapat dijadikan dasar pengembangan S. cumini sebagai antioksidan alami alternatif untuk mencegah kerusakan sel, kanker dan menurunkan kejadian penyakit degeneratif. Penelitian ini difokuskan pada S. cumini sebagai salah satu tanaman berkhasiat obat yang sudah mulai diteliti dalam khasiatnya sebagai tanaman yang mengandung senyawa bioaktif. Kehadiran teknologi GCMS ditengah belum adanya database tanaman jamblang merupakan peluang alternatif untuk memperoleh metabolom. Database metabolom pada jamblang penting dimiliki untuk menjaga plasma nutfah dari ekploitasi berlebihan yang menyebabkan kepunahan organisme dan budidaya tanaman tersebut. Dengan adanya penelitian inventarisasi level molekuler senyawa antioksidan yang dikandung tanaman jamblang dapat meningkatkan aktivitas penelitian lanjut pemanfaatan tanaman jamblang yang bisa ditingkatkan dalam skala industri nantinya. Untuk menjawab tujuan penelitian ini dilakukan tahapan penelitian sebagai berikut, pada tahap awal dilakukan isolasi metabolom dari kulit buah tanaman jamblang dengan cara ekstraksi. Tahap kedua adalah metabolome analysis menggunakan GCMS. Keluaran dari penelitian ini adalah diperolehnya data tentang hasil GCMS yang memuat senyawa bioaktif yang terkandung pada kulit buah tanaman jamblang yang akan menjadi landasan pengembangan pemanfaatan kulit buah jamblang sebagai antioksidan alternatif pencegah penyakit degenerasi. Pada penelitian selanjutnya perlu dilakukan analisis yang sama pada bagian tanaman jamblang lain untuk melengkapi database yang sudah ada.This research aims to create an antioxidant inventory databases of the Jamblang (Syzygium cumini) plant by Gas Chromatography-Mass Spectrometer (GCMS) approach.  This database can be used as the basis for the development of S. cumini as an alternative natural antioxidant to prevent cell damage, cancer and reduce disease incidence degenerative. This research is focused on S. cumini as one of the medicinal plants that have been studied in its usefulness as a plant containing the bioactive compound. The presence of GCMS technology amid the absence of Jamblang plant database is an alternative opportunity to obtain metabolome. The metabolome database in jamblang is important to keep the germplasm from excessive exploitation causing the extinction of the organism and the cultivation of the plant. Molecular level inventory of antioxidant compounds contained jamblang plants can increase research activities further utilization of jamblang plants that can be increased in the industrial scale later. To answer the purpose of this research carried out the following stages of research, in the early stages of metabolome isolation from the skin of jamblang fruit by way of extraction. The second stage is the metabolome analysis using GCMS. The output of this research is to obtain data about GCMS results that bioactive compounds contained in jamblang fruit skin that will become the foundation of the development of jamblang fruit skin as alternative antioxidant prevention of degeneration disease. In the next research needs to be done the same analysis on the jamblang plant to complete the existing database
Effect of Ethanol Extract of Jamblang Aceh (Syzygium cumini) in Diabetic Mice (Mus musculus) and Its Potential As Anti-Diabetic Agent Ayu  Nirmala Sari; Januardi Januardi; Diky Setya Diningrat
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.5496

Abstract

Abstract : This study was designed to evaluate the anti-diabetic activity of the ethanol extract of Syzygium cumini leaves in alloxan-induced diabetes mice. The anti-diabetic activity of EDS was investigated in mice (Mus musculus SW.) Alloxan-induced diabetes. The effect of ethanol extract of Syzygium cumini leaves on normal blood glucose levels and oral glucose tolerance tests were studied in normoglycemic mice while the anti-diabetic effect was evaluated in alloxan-induced hyperglycemic mice. Ethanol extract of Syzygium cumini leaves (200 and 400 mg/kg) is given orally for 21 days. Glibenclamide (5 mg/kg, oral for 21 days) is used as a reference standard. Giving ethanol extract of Syzygium leaves causes a significant decrease in blood glucose levels in normoglycemic and hyperglycemic mice and also increases glucose tolerance test. Ethanol extract of Syzygium leaves reduces glycosylated hemoglobin levels, lactate dehydrogenase, and creatinine kinase in alloxan-treated mice. Ethanol extract of Syzygium leaves also improves TBARS oxidative stress parameters, catalase and superoxide dismutase activity and glutathione levels. The ethanol extract of Syzygium cumini leaves shows anti-diabetic activity through increased insulin secretion and this effect can be attributed to the content of flavonoids and phenolic compounds present in the ethanol extract of Syzygium cumini leaves.Abstrak : Penelitian ini dirancang untuk mengevaluasi aktivitas anti-diabetes dari ekstrak etanol daun Syzygium cumini (L) Skeels (EDS) pada mencit diabetes yang diinduksi aloksan. Aktivitas anti-diabetes EDS diselidiki pada mencit (Mus musculus SW.) diabetes yang diinduksi aloksan. Pengaruh ekstrak etanol daun Syzygium cumini (EDS) pada kadar glukosa darah normal dan uji toleransi glukosa oral dipelajari pada mencit normoglikemik sedangkan efek antidiabetik dievaluasi pada mencit hiperglikemik yang diinduksi aloksan. EDS (200 dan 400 mg/kg) diberikan secara oral selama 21 hari. Glibenclamide (5mg/kg, oral selama 21 hari) digunakan sebagai standar referensi. Pemberian EDS menyebabkan penurunan signifikan dalam kadar glukosa darah pada mencit normoglikemik dan hiperglikemik dan juga meningkatkan uji toleransi glukosa. EDS mengurangi kadar hemoglobin glikosilasi, laktat dehidrogenase, dan kreatinin kinase pada mencit yang diberi aloksan. EDS juga memperbaiki parameter stres oksidatif TBARS, aktivitas katalase dan superoksida dismutase dan kadar glutathione. Ekstrak etanol daun Syzygium cumini (EDS) menunjukkan aktivitas antidiabetik melalui peningkatan sekresi insulin dan efek ini dapat dikaitkan dengan kandungan flavonoid dan senyawa fenolik yang ada dalam ekstrak daun.
Identifikasi Kandungan Senyawa Antioksidan Sebagai Agen Anti-Aging Pada Minyak Atsiri Cascara Kopi Robusta (Coffea canephora) Naomi Aprisa; Diky Setya Diningrat
Wahana-Bio: Jurnal Biologi dan Pembelajarannya Vol 18, No 1 (2026): WAHANA-BIO EDISI MEI 2026
Publisher : Program of Biology Education, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/wb.v18i1.24135

Abstract

Coffee cascara, often considered waste, contains bioactive compounds with antioxidant potential. This study aimed to identify antioxidant compounds in essential oil from Robusta coffee cascara green bean and evaluate their activity. Extraction was performed using the Soxhlet method with 96% ethanol, followed by GC-MS analysis, PubChem and PASS Online evaluation, and antioxidant testing using the DPPH method. A total of 20 compounds were identified, mainly volatile phenolics, alkaloids, and heterocyclics. Quinic acid showed the highest antioxidant potential, while the IC50 value of 15.78 mg/L indicated very strong antioxidant activity. These results suggest that cascara essential oil from Robusta coffee green bean is a promising anti-aging agent.
Inovasi Media Game Edukatif Sebagai Upaya Pencegahan Kepikunan Bagi Lansia di Sekolah Saroha Kota Medan Nurmala Berutu; Hodriani Hodriani; Diky Setya Diningrat; Ana Rahmi; Wan Nova Listia; Junaidi Junaidi
Abdimas Awang Long Vol. 9 No. 2 (2026): Juni, Abdimas Awang Long
Publisher : Sekolah Tinggi Ilmu Hukum Awang Long

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56301/awal.v9i2.2245

Abstract

Meningkatnya populasi lansia di Kelurahan Bandar Selamat belum diimbangi dengan pembelajaran berbasis stimulasi kognitif, karena program lansia masih didominasi pendekatan kesehatan fisik dan metode konvensional yang kurang interaktif. Kegiatan ini bertujuan mengembangkan media game edukatif yang mengintegrasikan stimulasi kognitif, aktivitas rekreatif, dan interaksi sosial sebagai strategi pencegahan penurunan fungsi kognitif lansia di Sekolah Saroha Kota Medan. Metode pelaksanaan menggunakan pendekatan Participatory Action Research (PAR) melalui observasi, Focus Group Discussion (FGD), pengembangan media, implementasi game edukatif, serta evaluasi partisipatif untuk menghasilkan pembelajaran lansia yang adaptif dan berkelanjutan. Hasil menunjukkan bahwa pembelajaran di Sekolah Lansia Saroha sebelumnya masih didominasi metode konvensional sehingga stimulasi kognitif dan keterlibatan aktif lansia belum optimal. Kegiatan ini berhasil mengembangkan enam media game edukatif berbasis elderly-friendly, yaitu ular tangga, board game, teka-teki silang, tebak gambar, domino, dan engklek edukatif yang dirancang untuk meningkatkan memori, konsentrasi, interaksi sosial, dan partisipasi belajar lansia. Implementasi media menunjukkan bahwa lansia menjadi lebih aktif, komunikatif, antusias, dan percaya diri dalam mengikuti proses pembelajaran berbasis kelompok. Selain itu, pelatihan fasilitator berhasil meningkatkan kapasitas pendamping dalam menerapkan pembelajaran yang lebih interaktif, adaptif, dan menyenangkan bagi lansia. Secara keseluruhan, media game edukatif terbukti berpotensi menjadi inovasi pembelajaran berbasis komunitas yang efektif dalam mendukung stimulasi kognitif, active aging, dan pencegahan penurunan fungsi kognitif lansia di Sekolah Lansia Saroha Kota Medan.
Keterampilan Proses Sains dan Berfikir Tingkat Tinggi Siswa melalui Model Inductive Thinking dan Group Investigation Siska Aidarahmi; Binari Manurung; Diky Setya Diningrat
Ideguru: Jurnal Karya Ilmiah Guru Vol. 9 No. 2 (2024): Edisi Mei 2024
Publisher : Dinas Pendidikan, Pemuda dan Olahraga Daerah Istimewa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51169/ideguru.v9i2.853

Abstract

This research aims to determine the science process and high-level thinking of class X high school students who are given an inductive thinking and group investigation learning model. This research was carried out in April-June at MAN 1 Aceh Tenggara. The research population was all class X MIA students of 300 people. The sample was taken by purposive sampling and amounted to 60 people. The research results show that (1) There is a very significant difference in science process skills and learning models. The results of science process skills taught using the investigative group learning model (88.83 ± 8.37) were more significant than the inductive thinking learning model (85.50 ± 7.23); (2) There is a very significant difference between high level thinking and learning models. The results of higher level thinking taught using the investigative group learning model (87.33 ± 13.37) were more significant than the inductive thinking learning model (83.33 ± 12.41); (3) There are differences in science process skills given the inductive thinking learning model and group investigation at low and high KBA; (4) There are differences in high-level thinking given the inductive thinking learning model and group investigation at low and high KBA; (5) There is a relationship between science process skills and students' higher level thinking from the results of applying the inductive thinking learning model and group investigation, r = 0.876 and r^2 = 0.768.
Analisis Fitokimia Dan Aktivitas Antioksidan Minyak Atsiri Cascara Kopi Robusta (Coffea canephora) Anggriani Br. Pasaribu; Diky Setya Diningrat
CERATA Jurnal Ilmu Farmasi Vol 16 No 2 (2025): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v16i2.1754

Abstract

Penelitian ini dilatarbelakangi oleh kurangnya informasi spesifik mengenai kandungan senyawa fitokimia serta aktivitas antioksidan dari minyak atsiri cascara kopi Robusta (Coffea canephora). Cascara, sebagai limbah pengolahan kopi, umumnya belum dimanfaatkan secara maksimal dan sering menimbulkan permasalahan lingkungan akibat akumulasi limbah organik. Padahal, diketahui cascara memiliki kandungan senyawa bioaktif, termasuk antioksidan, namun aplikasinya masih terbatas, terutama dalam bentuk minyak atsiri. Proses destilasi uap yang semula direncanakan tidak memberikan rendemen optimal, sehingga digunakan metode soxhletasi dengan pelarut etanol sebagai alternatif. Penelitian ini bertujuan mengidentifikasi senyawa fitokimia dalam minyak atsiri cascara dengan teknik GC-MS dan mengevaluasi aktivitas antioksidannya menggunakan uji DPPH. Cascara kering sebanyak 200 gram diekstraksi dan menghasilkan minyak atsiri sebanyak 8 ml (rendemen 4,75%). Analisis GC-MS mengungkapkan 17 senyawa, dengan kafein sebagai komponen dominan (62,12%). Bioaktivitas senyawa yang terdeteksi dianalisis melalui PubChem, dan menunjukkan beragam aktivitas seperti antioksidan, antiinflamasi, antikanker, dan antibakteri. Uji DPPH menunjukkan kemampuan antioksidan yang kuat, dengan persen inhibisi >50% pada konsentrasi 80 mg/L dan nilai IC50 dalam kategori kuat. Hasil ini menunjukkan bahwa minyak atsiri cascara kopi Robusta berpotensi sebagai sumber antioksidan alami yang aplikatif di bidang pangan, farmasi, dan kosmetik.
Pengembangan E-LKPD Keanekaragaman Hayati Berbasis Proyek untuk Meningkatkan Keterampilan Abad 21 Siswa dalam Mendukung Pencapaian SDGs Anliana; Diky Setya Diningrat; Aswarina Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 9 No. 3 (2026): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/nfdfy487

Abstract

This study aimed to develop project-based digital worksheets (E-LKPD) integrated with the Sustainable Development Goals (SDGs) on the topic of biodiversity to enhance students’ 21st-century skills (4Cs). The method used was Research and Development (R&D) with the 4D model through stages of expert validation, interviews, and questionnaires, as well as data analysis using validity tests, N-Gain, and t-tests. The results showed that the E-LKPD had a very high feasibility level (≥92%) and was effective in improving 4C skills, ranging from moderately effective to effective across all aspects. The t-test revealed a significant difference between pretest and posttest results (Sig. < 0.001). Additionally, the E-LKPD was rated as very engaging by students (91.60%) and very practical by teachers (95.70%). Thus, PjBL-based E-LKPD integrated with the SDGs is feasible and effective for use in biodiversity education to enhance 21st-century skills.  Keywords: Biodiversity, E-LKPD, Project-Based Learning (PjBL), SDGs, 4C Skills
Co-Authors -, Kusdianti Abdul Muin Sibuea Adelia Febriyossa Adi Hartono Adi Hartono Agustina, Rezeki Sri Aini Fitri Ana Rahmi Ana Rahmi Angga Karisto Bangun Anggriani Br. Pasaribu Anliana Aprisa, Naomi Ashar Hasairin Ashar Hasairin Ashar Hasairin Asmin Asmin, Asmin Aswarina Nasution Ayu Nirmala Sari Ayu Nirmala Sari Bangun, Angga Karisto Binari Manurung Binari Manurung Budiarta, Kustoro Dewi, Gustianti Erly Marwani Erly Marwani, Erly Fadilah Ismail Fahmi, M. Haikal Fahri Setya Maulana Faisal Faisal Faisal Faisal Faizah, Siti Fajrin, Rahmat Farhana Setya Farhana Farhana, Farhana Setya Fatma Tresno Ingtyas Fitriani, Sonia Fiyantara, Aljun Ginting, Alin Anggreni Gustianti Dewi Harahap, Novita Sari Hartono, Adi Hasairin, Ashar Hasibuan, Nurfadilah Azizah Hasibuan, Safrina Rizkiah Heppy Setya Prima Herbert Sipahutar, Herbert Hodriani Hodriani Ingtyas, Fatma Tresno Jamaludin, Askiah Binti Januardi Januardi Janwardi Reywanda Tarigan Junaidi Junaidi Junaidi Junaidi Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti laela Sari, laela Lumbantoruan, Rio Tresia Maulana, Bagoes Maulana, Fahri Setya Muhammad Azhari Muliawati Muliawati Muslich Hidayat Naomi Aprisa Nasution, Risna Lenita Novita Sari Harahap Nurmala Berutu Nurmala Berutu Pasaribu, Anggraini Br. Pitaloka, Cindy Purba, Febi Anjaini Putriana Purba Rahmat Fajrin Rahmi, Anna Rajagukguk, Teresia Junetti Ramadanu Ramadanu Ramadanu, Ramadanu Restuati, Martina Salleh, Rabeta Mohd Samosir, Cresfo Sinaga, Acong Jaya Sinurat, Asri Sipayung, Gratia Anggita Siregar, Batumahadi Siregar, Zulkarnain Siska Aidarahmi Sonya Adelina Sipayung Sri Winarsih Syafrina Sari Lubis Tarigan, Janwardi Reywanda Ummi Zaimah Wahyuni Lubis Wan Nova Listia Yetty Machrina Zulaini Zulaini Zulaini, Zulaini