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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan journal of Basic Medical Veterinary Current Biomedicine
Tyasningsih, Wiwiek
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Detection of Contamination Salmonella sp. of Beef in Banyuwangi Traditional Market Hanifa, Muril Hizriyana; Tyasningsih, Wiwiek; Yunita, Maya Nurwantanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.247-251

Abstract

Salmonella sp. is one of the bacteria from Food-Borne Disease agents, can contaminate meat and cause salmonellosis. According to National Indonesian Standard 2009, meat is safe for consumption if it has a negative result of salmonella in 25 grams of meat. The purpose of this study was to determine whether there was contamination of Salmonella sp. on beef sold in the traditional market of Banyuwangi District. A total of 18 samples used in this study were taken according to the criteria for isolation and identification. All samples were enriched with the pre-enrichment stage using Lactose Broth media, and the enrichment medium using Tetrathionate Broth. Furthermore, in the isolation stage using Salmonella Shigella Agar (SSA) media. Then proceed with the identification stage using the Triple Sugar Iron Agar (TSIA) test, the Sulfide Indole Motility (SIM) test, and the Urease test. The results showed that 5 out of 18 samples tested positive for Salmonella.
Risk Factors for Feline Otitis in Madiun: A Prospective Study Khasana, Uswatun; Meles, Dewa Ketut; Praja, Ratih Novita; Tyasningsih, Wiwiek; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.29-34

Abstract

This study aimed to determine the risk factors in cats associated with the incidence of otitis. A total of 169 cat samples were evaluated by filling out questionnaires and interviewing owners, then an examination was carried out to determine symptoms and treatment so that appropriate action could be taken. Data were analyzed descriptively and tested using Chi-Square analysis. As a result, there were 26 (15,4%) cats diagnosed with otitis. The results of the analysis of significant risk factors include bathing frequency, ear hygiene, and maintenance patterns that can increase the incidence of otitis. The most common clinical symptoms are pruritus, head shaking and accompanied by earwax. The description of the statement about otitis concluded that as many as 70 cat owners, most of them did not understand otitis.
Retrospective Study of Feline Panleukopenia Risk Factors in Pet Cats in Madiun Baroroh, Diana Nova; Tyasningsih, Wiwiek; Praja, Ratih Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.114-119

Abstract

This study analyzed the association between risk factors and Feline panleukopenia at the Ichiyo Veterinary Clinic, Madiun using a retrospective method. A total of 2037 data used was secondary data obtained from cat medical and ambulatory reports from January 2018-March 2021. The risk factors analyzed included age, gender, vaccination status, comorbid diseases, housing system, number of cats, and season. Data was presented descriptively and analyzed using Chi-Square analysis. The results showed that the risk factors for Feline panleukopenia were cats aged ≤6 months, cats that were not vaccinated, and cats with comorbid diseases. It can be concluded that prevention of Feline panleukopenia can focus on age, vaccination and treatment of potential comorbid diseases in cats.
Identification of Staphylococcus sp. and Antibiotic Resistance in Tutur District, Pasuruan Purnamasari, Indah; Suwarno, Suwarno; Tyasningsih, Wiwiek
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.93-104

Abstract

This study aimed to identify Staphylococcus sp. and antibiotic resistance in Tutur District, Pasuruan. A total of 273 samples of cow's milk were evaluated and identified as Staphylococcus sp. Furthermore, the antibiotic resistance test was carried out according to CLSI standards. This study showed 41 isolates of Staphylococcus sp. of 97 positive CMT. The results of the study reported seven isolates of Staphylococcus sp. resistant to antibiotics consisted of one multidrug-resistant isolate, i.e. S. saprophyticus which was resistant to penicillin, erythromycin and clindamycin, and six antibiotic resistant isolates consisted of two S. aureus isolates, one S. haemolyticus isolate and three isolates S. simulans. Staphylococcus sp. isolates showed resistance to penicillin, erythromycin, clindamycin, and tetracycline. While the antibiotics gentamicin, ciprofloxacin, cefoxitin, and cotrimoxazole did not reported resistance, so these antibiotics were applicable. The incidence of bacterial resistance Staphylococcus sp. to antibiotics with details of resistance to penicillin 7,3%, resistance to tetracycline 7,3%, resistance to clindamycin 4,8% and resistance to erythromycin 4,8%.
Pengaruh Aflatoksin 9,58 ppb dalam Pakan Terhadap Biometrik Saluran Cerna dan Berat Badan Ayam Petelur Periode Awal Pertumbuhan Pratama, Dio Putra; Tehupuring, Benjamin Chr.; Poetranto, Emmanuel Djoko; Eliyani, Hana; Soeharsono, Soeharsono; Tyasningsih, Wiwiek; Hendarti, Gracia Angelina
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28585

Abstract

The purpose of this study was to determine the effect of aflatoxin content of 9.58 ppb in feed on gastrointestinal biometrics and weight gain of laying hens in the early growth period. Thirty-six day old chicks (DOC) were used in this study and were divided into two groups P0 and P1. P0 (control group) was fed with 100% basal diet and P1 (treatment group) was fed a diet with a composition of 80% basal feed + 20% aflatoxin-containing feed. After the DOC was adapted, the first feeding containing aflatoxin was given from the 20th day to the 60th day. Data collection was carried out three times, namely the 20th, 40th, and 60th days. At the end of each period, the chickens would be euthanized to collect data on the weight and length of the digestive tract and body weight. Weight gain with time increased, but had no significant effect (p>0.05) between the control and treatment groups. Body weight continues to increase because the body's homeresis function is still running well. Observations on gastrointestinal biometrics showed significant differences (p<0.05) in oesophagus weight, proventriculus weight, ventriculus weight, jejunum weight, ileum weight and length, and cecum weight because aflatoxins have a direct effect on the digestive tract they pass. The conclusion of this study was that the provision of 20% aflatoxin-containing feed did not affect weight gain but had shown an effect on the biometrics of several gastrointestinal tracts.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Darmawan, Moch. Rizky; Rahardjo, Dadik; Tyasningsih, Wiwiek; Kurnijasanti, Rochmah; Legowo, Djoko; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
The Implementation of Hazard Analysis and Critical Control Point (HACCP) in The Frozen Chicken Meat Distribution Chain in Sikka Regency Dua Bura, Maria Antonia Yersi; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Rahardjo, Dadik; Tyasningsih, Wiwiek; Rachmawati, Kadek
VITEK : Bidang Kedokteran Hewan Vol 14 No 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.289

Abstract

Food safety has become a global issue. Hazard analysis and critical control points (HACCP) is a food safety assurance system in the world that emphasizes prevention efforts. This research is a qualitative descriptive epidemiological study which aims to examine the application of HACCP in the distribution chain of frozen chicken meat in Sikka Regency on the growth parameters of total bacteria, Salmonella, and antibiotic residues through field observations at supplier companies and retailers, as well as laboratory tests on 30 frozen chicken meat samples, and 30 fresh chicken meat samples as a comparison. Laboratory tests found antibiotic residues in 60% of samples (16.7% in fresh chicken meat), and microbial contamination in 33% of samples without Salmonella contamination (40% in fresh chicken meat without Salmonella contamination). Monitoring of antibiotic residues has not been carried out properly by supplier companies because the number of samples is very small, sales in markets without refrigeration facilities for up to five hours together with fresh chicken or near fresh fish allows contamination of chicken meat. HACCP principles have not been properly applied in the frozen chicken meat distribution chain. Guidance and regular monitoring are needed so that the quality of frozen chicken meat is maintained.
First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
Co-Authors Amelia, Ezanti Nur Amrullah, Amirul Muslim Baroroh, Diana Nova Boedi Setiawan Chusniati, Sri Dadik Rahardjo, Dadik Darmawan, Moch. Rizky Dua Bura, Maria Antonia Yersi Dwi, Welyanto Kurniawan Erma Safitri Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Hamid, Iwan Sharial Hana Eliyani Hanifa, Muril Hizriyana Harijani, Nenny Hayati, Laila Nur Hendarti, Gracia Angelina Indah Purnamasari Indriyani, Dwi Putri Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khasana, Uswatun Kusuma, Agung Jati Kusumastuti, Imbi Legowo, Djoko Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Murjianingsih, Fitriani Mustofa Helmi Effendi Nurwartanti, Maya Permatasari, Dian Ayu Poetranto, Emmanuel Djoko Praja, Ratih Novita Prameswari, Ratna Ajeng Pratama, Dio Putra Rahadyan, Raihan Alif Raharjo, Dadik Rahmahani, Jola Ramandinianto, Sancaka Chasyer Rochmah Kurnijasanti Saputro, Amung Logam Sarudji, Suryanie Soeharsono Soeharsono Suwarno Suwarno Tacharina, Martia Rani Tehupuring, Benjamin Chr. Tiara Wandira Hariyanto Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwantanti Yunita, Maya Nurwartanti