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Luqman, Epy Muhammad
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Journal : journal of Basic Medical Veterinary

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EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Purnomo, Edi; Luqman, Epy Muhammad; Ratnani, Hermin; Plumeriastuti, Hani; Mafruchati, Maslichah; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
Erythrocyte, Hemoglobin, and Hematocrit Profiles in Indonesian Simental and Limousin in Highlands and Lowlands in Banyuwangi Trisnawati, Sharfina Habibah; Safitri, Erma; Agustono, Bodhi; Luqman, Epy Muhammad; Saputro, Amung Logam; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.78209

Abstract

This study aimed to evaluate the differences in blood profile of erythrocytes, hemoglobin, and PCV in Indonesian simental and limousin beef cattle in highlands and lowlands. Banyuwangi is one of the beef cattle contributors in East Java with its highland and lowland topography. This research was conducted from July to August 2023. The research was conducted in 3 locations namely highlands (Kalibaru), lowlands (Rogojampi) and sample examination in Airlangga University Banyuwangi. Animals used in this study were Indonesian simental and limousin beef cattle with 1-2 years of age as many as 15 heads in the highlands and 15 heads in the lowlands. The research method used was purposive sampling. Blood samples used in this study used Indonesian simental and limousin beef cattle blood taken using venoject and inserted in EDTA tubes and examined using a Hematology Analyzer. The variables observed in this study were the number of erythrocytes, hemoglobin, and PCV. The data obtained were processed with Independent T-test data analysis. The results showed no significant difference in erythrocytes and PCV, but there was a significant difference in hemoglobin levels. The conclusion of this study is that Indonesian simental and limousin beef cattle in the lowlands experience mild anemia.
Co-Authors AA Sudharmawan, AA Agustono, Bodhi Aisyah R.r, Herini Siti Al Arif, Muhammad Anam Al-Arif, Muhammad Anam Arimbi Berlina, Cyrcilia Relita Chairul Anwar Chandra, Evania Haris Dikman, Dicky Mohammad Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Hani Plumeriastuti Heni Puspitasari Hermin Ratnani Hidanah, Sri Iwan Sahrial Hamid Jeanita, Winny Kurniawan, Muhammad ‘Ahdi Lilik Maslachah Mafruchati, Maslichah Malae, Rafyan Meles, Dewa Ketut Mufasirin Nursadida, Milla Pramudita, Kautsar Pandu Prastiya, Ragil Angga Pudji Srianto Rianda, Juan Safero Rusyawardani, Aldis Ingrid Sabdoningrum, Emy Koestanti Saputro, Amung Logam Seagames Waluyo, Seagames Sri Pantja Madyawati, Sri Pantja Sugiyono Suparto Wijoyo Supriyadi Supriyadi Susilowati, Suheni Suzanita Utama Tita Damayanti Lestari Tri Wahyu Suprayogi Trisnawati, Sharfina Habibah Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Yeni Dhamayanti Zariyah, Aminatus