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All Journal AL KAUNIYAH Jurnal Kesehatan Andalas Mutiara Medika: Jurnal Kedokteran dan Kesehatan Majalah Kesehatan FKUB Medical Laboratory Technology Journal Jurnal Surya Medika PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kedokteran Universitas Palangka Raya Pengabdian Kampus: Jurnal Informasi Kegiatan Pengabdian Pada Masyarakat JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Barigas: Jurnal Riset Mahasiswa
Permatasari, Silvani
Department Of Biology, Faculty Of Medicine, Universitas Palangka Raya, Palangka Raya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : Medical Laboratory Technology Journal

 1 
Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility Silvani Permatasari; Nida Halisa; Agnes Frethernety
Medical Laboratory Technology Journal Vol. 9 No. 2 (2023): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i2.563

Abstract

Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.
The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
Co-Authors Agnes Frethernety Agnes Frethernety Alexandra, Francisca Diana Andreyan Cesarianto Anna Marthea Veronica Aprilliana, Riska Arini Ratnasari Arnino A Astri Widiarti Astrid Teresa ASTRID TERESA Balyas, Abi Bakring Berliani, Victoria Chandra Chandra Dea Tutut Dede Z. A Dewi Klarita Furtuna Dewi Klarita Furtuna Dhaniang, Natasya Dwi Ari Pujianto Elsa Trinovita Ervi Audina Munthe Fadhiyatulqalbi Ahmad, Farah Fauziyah Fatmaria, Fatmaria Febrianto E. P Florence Felicia Florence Felicia Fraulein Aryati Fraulein Aryati Frethernety, Agnes Furtuna, Dewi Klarita Furtuna, Dwi Klarita Galih Indra Permana Hairunnida Rahmatina Halisa, Nida Handayani, Septi Herlina Eka Shinta I Gde Eka A Idsan, Rakha Satya Indah Noniika Indria Augustina Jhon Paris Pasaribu Kahanjak, Donna Novina Krestina, Widya Lia Sasmithae Malasinta Malasinta Malinda, Okta Mallewa, Nurul Ilmi Marliah, Dinda Masaris Mery Irayanti Misbah Misbah Muhammad Aldri Muntir Guru Singa Nabila Putri Aditya Natalia Resoldes LBN Tobing Natalia Sri Martani Nawan, Nawan Ni Putu Saraswati Ni Putu W.S.W Nida Halisa Nuha, Alya Firyal Nurfianto, Sigit Oktaviannur, Zoelva Patricia, Tisha Rahmidiati Ramadhani, Ahmad Ratna Widayati Ratna Widayati Ria Eriani Rian Ka Praja Rini Fitri R Seiya, Saint Septi Handayani Sherly Marselina Sitorus, Elisabeth Prihana Rotua Syamsul Arifin Syarpin Syarpin Syarpin Tangdibali, Annisa Zaskia Alexandra Tasya, Tasya Theressia Angel Lady Saragih Tisha Patricia Toemon, Agnes Immanuela Tri Widodo Vani Vrenika Widayati, Ratna Widya Krestina Yanuardi Ikhsan Yenisa Jestin Ganindyatama Ysrafil, Ysrafil