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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Walisongo Journal of Chemistry INTEK: Jurnal Penelitian Jurnal Mandala Pharmacon Indonesia Health Information : Jurnal Penelitian Budimas : Jurnal Pengabdian Masyarakat Analit: Analytical and Environmental Chemistry Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment MEDICA (International Medical Scientific Journal) Analit: Analytical And Environmental Chemistry
Ratnawaty, Gervacia Jenny
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Effectiveness of Moringa oleifera and PAC (Poly Aluminum Cloride) Leaves in Peat Organic Substances in Rasau Jaya Village, Kubu Raya Regency Ratnawaty, Gervacia Jenny; Salim, Maulidiyah
INTEK: Jurnal Penelitian Vol 8 No 1 (2021): April 2021
Publisher : Politeknik Negeri Ujung Pandang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31963/intek.v8i1.2729

Abstract

One source of surface water in West Kalimantan is peat water that has low turbidity, dark brown to black (124 - 850 units of PtCo), high organic content (138-1560 mg / L KMnO4), and is acidic (pH 3,7 - 5,3). The purpose of this study was to determine the effect of the addition of PAC and Moringa oleifera leaves to the decrease in levels of peat organic water in the Rasau Jaya village area, Kubu Raya Regency. The method used in this research is by mixing Moringa leaf powder and PAC (Poly Aluminum Cloride) in peat water accompanied by stirring and precipitating. Furthermore, peat water samples were analyzed for their organic content using the permanganometric titration method. The results showed that the levels of organic substances in peat water before the addition of PAC and Moringa leaf powder amounted to 176.96 mg / l, after the addition of 125 mg PAC and Moringa leaf powder with concentrations of 25, 50, 75 l , 100, 125, 150, 175, 200 mg/l, respectively: 42.04 mg / l, 46.97 mg / l, 58.14 mg / l, 66.15 mg / l, 67.20 mg / l, 60.25 mg / l, 63.41 mg / l, and 53.07 mg / l. Based on the results through regression statistical tests obtained sig = 0.015 <0.05 which means Ha is accepted that there is an effect of increasing the concentration of Moringa oleifera powder and PAC  on Organic Content in Peat Water in Rasau Jaya Village, Kubu Raya Regency.
Identification and Analysis of Active Carotenoid Compounds From Entawak Fruit (Artocarpus Anisophyllus) Ratnawaty, Gervacia Jenny; Indrawati, Ratih; Syari, Jajar Pramata
INTEK: Jurnal Penelitian Vol 8 No 2 (2021): October 2021
Publisher : Politeknik Negeri Ujung Pandang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31963/intek.v8i2.2984

Abstract

— Isolation and identification of active compounds from mentawak fruit (Artocarpus Anisophyllus) used a plant determination test to determine the plant content. Furthermore, the extraction process was carried out to obtain a crude extract of carotenoid pigments by maceration method using n-hexane as solvent. Furthermore, the obtained macerate was carried out by phytochemical tests to determine the content of the active compounds. The TLC test was carried out to confirm the presence of a positive group of compounds on phytochemical screening, and to determine the chromatographic profile of the extract.  The identification results showed that the phytochemicals showed that the active compounds contained in macerate were alkaloids, flavonoids, tannins and saponins. The KLT test results obtained an Rf value of 0.875. While the total carotenoid content using a spectrophotometer UV-Vis is 958 µg/ml.
Senggani Fruit Anthocyanins (Melastoma Malabathricum Auct, Non Linn) As Bacterial Dyes Differential Painting Techniques Indrawati, Ratih; Ratnawati, Gervacia Jenny; Tumpuk, Sri
INTEK: Jurnal Penelitian Vol 9 No 1 (2022): April 2022
Publisher : Politeknik Negeri Ujung Pandang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31963/intek.v9i1.2987

Abstract

Bacteria are difficult to see with a light microscope, because they do not absorb or refract light. Dyes absorb and refract light so that the contrast of bacteria with their surroundings is enhanced. Anthocyanins are water-soluble pigments that are naturally found in various types of plants. As the name implies, this pigment gives color to flowers, fruits, and leaves of green plants.This research is a type of descriptive research. The treatment consisted of painting bacterial preparations with anthocyanin pigment extract of senggani fruit with 70% ethanol solvent and control with gram staining. The treatments were: T1: staining of bacterial preparations with gram staining, T2: painting of bacterial preparations with anthocyanin extract of senggani fruit added with 14% citric acid as a substitute for safranin and NH4Cl as a substitute for crystal violet. The results of the study showed that anthocyanin pigment extract was proven to be used as a dye for Staphylococcus aureus, Escereria coli as a substitute for synthetic dyes Safranin and Crystal violet in Gram staining.
Utilization of Red Dragon Fruit (Hylocereus polyrhizus) as an Alternative Dye for Threadworm Eggs (Ascaris lumbricoides) Erfiana, Desti; Nurhayati, Etiek; Ratnawaty, Gervacia Jenny
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.483

Abstract

The red dragon fruit (Hylocereus polyrhizus) contains a pigment called anthocyanin, which gives it its red color and has the potential to be used as a natural dye. In laboratory microscopic preparations, eosin is used as a standard dye. Natural dyes have the advantage of not causing negative effects like cancer or environmental damage. This study aimed to examine the potential of red dragon fruit (Hylocereus polyrhizus) as an alternative dye for the examination of Ascaris lumbricoides eggs. The study design used was static group comparison, with local red dragon fruit samples from Pontianak Tenggara District. Laboratory examination methods involved direct microscopic observation using a 2% eosin control. In this study, the juice from both the skin and flesh of the red dragon fruit was extracted and prepared in solutions of 80%, 90%, and 100% concentrations with four replications. The results showed that the skin of the red dragon fruit at concentrations of 80%, 90%, and 100% was not suitable for coloring the microscopic preparation for observing worm eggs. However, the juice from the flesh of the red dragon fruit at concentrations of 80%, 90%, and 100% could color the microscopic preparations, and the Ascaris lumbricoides eggs could be identified. At the 80% concentration of red dragon fruit flesh, the average score was 2, indicating low contrast background, weak color absorption by the eggs, and unclear egg details. At 90% and 100% concentrations, the average score was 3, indicating a clear contrast background, good color absorption by the eggs, and clear egg detail, similar to eosin 2% staining. This study concludes that the skin of red dragon fruit cannot be used for staining microscopic preparations, while the flesh of red dragon fruit can be used for staining Ascaris lumbricoides eggs, with optimal concentrations of 90% and 100%.
Phytochemical Identification and Antioxidant Activity Test of Acetone Extract of Celery Leaves (Apium graveolens L.) Using the DPPH (2,2-diphenyl-1-picrylhydrazyl) Method Auliannisa, Riska; Jenny, Gervacia; Djohan, Herlinda
MEDICA (International Medical Scientific Journal) Vol. 6 No. 3 (2024): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i3.488

Abstract

Free radicals are formed in the body through oxidation processes and cell combustion during breathing, metabolism, excessive physical activity, as well as exposure to external pollution. Antioxidants play an important role in protecting the body from oxidative damage. Celery leaves (Apium graveolens L.), which are rich in flavonoid compounds, have the potential to be a natural source of antioxidants. This study aims to identify the secondary metabolite content and measure the antioxidant activity of acetone extract of celery leaves based on IC50 values using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The study uses a quasi-experimental design with purposive sampling techniques. The antioxidant activity of acetone extract of celery leaves at concentrations of 30 ppm, 50 ppm, 70 ppm, and 90 ppm was tested using a UV-VIS spectrophotometer at a wavelength of 516 nm. The results showed the presence of secondary metabolite compounds such as alkaloids, flavonoids, phenols, triterpenoids, saponins, and tannins in the acetone extract of celery leaves. The IC50 value of the acetone extract of celery leaves was 115.09490 ppm, indicating moderate antioxidant activity (IC50 value between 100–250 ppm). These findings conclude that acetone extract of celery leaves has potential to be used as a natural antioxidant source.
Influence pH to Rate Anthocyanins Extract Senggani Fruit ( Melastoma malabathricum Auct, non Linn) Indrawati, Ratih; Jenny.R, Gervacia; Salim, Maulidiyah; Ihsan, Bagus Muhammad; Widiyantoro, Ari
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7267

Abstract

One of the plants that can be used as a natural dye is senggani fruit. This study aimed to determine the effect of acid pH on the anthocyanin levels of the senggani fruit extract. The method used in this study uses UV-Vis Spectrophotometry. The sample was senggani fruit extract, added with an acidic pH solution (1-6) with 3 repetitions for 18 samples. Based on the results of research that has been carried out, the average anthocyanin levels at pH 1 are 2.00% with a bright red color; pH 2 is 1.63% with a red-orange color, pH 3 is 1.45% with an orange color, pH 4 is 2.05 % with brownish red color, pH 5 is 1.38% with brownish orange color, and pH 6 is 1.11% with light brown color. Based on statistical tests using Simple Linear Regression, Ha was accepted so that it could be concluded that there was an effect of acid pH on the anthocyanin levels of the senggani fruit. Based on a study that has been done, it is known that the sourer the pH solution is, the taller the anthocyanin obtained. Matter happens Because absorbance is comparable with the rate of anthocyanin something sample.
PENYULUHAN PENGGUNAAN PEWARNA ALAMI DARI DAUN KELOR (Moringa oleifera) SEBAGAI SUMBER ANTIOKSIDAN PADA MAKANAN DALAM RANGKA MENINGKATKAN NILAI GIZI MASYARAKAT Ratnawaty, Gervacia Jenny; Indrawati, Ratih; Sungkawa, Hendra Budi
BUDIMAS : JURNAL PENGABDIAN MASYARAKAT Vol. 6 No. 1 (2024): BUDIMAS : Jurnal Pengabdian Masyarakat
Publisher : LPPM ITB AAS Indonesia Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanaman Kelor telah dikenal selama berabad-abad sebagai tanaman multi guna, padat nutrisi dan berkhasiat obat. Mengandung senyawa alami yang lebih banyak dan beragam dibanding jenis tanaman lainnya yang ada. Tanaman Kelor mengandung 46 anti oksidan kuat yang melindungi tubuh dari radikal bebas, mengandung 18 asam amino (8 diantaranya esensial) yang dibutuhkan tubuh untuk membangun sel-sel baru, 36 senyawa anti inflamasi, serta 90 nutrisi alami seperti vitamin dan mineral . Kelor merupakan bahan pangan yang kaya akan zat gizi makro dan mikro. Kandungan nilai gizi yang tinggi dalam daun kelor dapat dimanfaatkan untuk pemenuhan kebutuhan nutrisi pada ibu menyusui dan balita dalam masa pertumbuhan (Aminah et al, 2015). Pemberian daun kelor dapat meningkatkan Indeks Masa Tubuh (IMT) pada balita (Rahayu & Nurindahsari, 2018). Penelitian yang pernah dilakukan oleh Sari,(2019) menunjukkan bahwa penambahan tepung daun kelor yang lebih tinggi akan meningkatkan kadar abu, kadar protein dan kadar serat kasar pada tepung komposit. Selain itu daun kelor juga mengandung antioksidan yang tinggi yaitu pada bagian atas atau pucuk daun (SUGIANTO, 2016) . Metode pelaksanaan yang dilakukan adalah kunjungan ke lokasi mitra, demontrasi pelatihan pembuatan hasil olahan makanan menggunakan daun kelor, dan sosialisasi tentang kandungan gizi daun kelor di Desa Rasau Jaya Umum, Kabupaten Kubu Raya Kalimantan Barat.
ENKAPSULASI DAN STABILITAS PIGMEN KAROTENOID DARI BUAH ENTAWAK (Artocarpus Anisophyllus) Ratnawaty, Gervacia Jenny; Indrawati, Ratih
Analit : Analytical and Environmental Chemistry Vol. 8, No. 01 April (2023) Analit: Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v8i1.2023.p77-87

Abstract

Pada penelitian ini telah dilakukan proses enkapsulasi dan stabilitas pigmen karotenoid dari buah entawak (Arthocarpus Anisophyllus). Konsentrasi Maltodekstrin sebagai penyalut yang paling efisien adalah 10%. Berdasarkan uji statistic SPSS menggunakan uji korelasi dari hasil penelitian diperoleh untuk kecepatan pengadukan terdapat pengaruh yang signifikan antara kecepatan pengadukan terhadap kadar karotenoid dengan nilai signifikansi (p) 0,000 < 0,05. Pada Uji termostabilitas yang meliputi pH dan suhu diperoleh untuk uji pH diperoleh nilai signifikan(p) 0,111 > 0,05 yang artinya tidak terdapat pengaruh kadar karotenoid terhadap pH. Dan nilai signifikansi (p) 0,871 < 0,05 artinya tidak ada hubungan yang signifikan antara kadar karotenoid terhadap suhu. Sementara uji fotostanilitas yang meliputi pengaruh sinar matahari dan Sinar UV-Vis diperoleh nilai signifikansi (p) 0,047 < 0,05, dan nilai signifikansi (p) 0,008 < 0,05 yang artinya terdapat pengaruh kadar karotenoid terhadap UV-VIS. Kata kunci: Enkapsulasi, Uji Stabilitas, Buah Entawak .
Antibacterial Activity of Ethanol Extract Ointment of Tithonia diversifolia Against Staphylococcus aureus Triana, Linda; Supardi, Rizal; Sutriswanto, Sutriswanto; Ratnawati, Gervacia Jenny; Sari, Emilda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2136

Abstract

Skin infections caused by pathogenic bacteria such as Staphylococcus aureus remain a common health problem, and the need for safer alternative topical antibacterial agents continues to increase. This study aimed to evaluate the antibacterial activity of ethanol extract ointment of Tithonia diversifolia leaves with various concentrations formulated as a semi-solid topical preparation. A quasi-experimental design was used, and ointments were prepared at concentrations of 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, and 21%. Antibacterial activity was tested using the well-diffusion method against Staphylococcus aureus. A total of 27 samples were tested with three replications. The results showed that the ointment produced inhibition zones ranging from 13.83 mm to 22.00 mm, with higher concentrations yielding greater inhibition. Repeated Measures ANOVA showed a significant difference among concentrations (p = 0.003), indicating that the increase in extract concentration significantly affected the antibacterial effectiveness. This study concludes that ethanol extract ointment of Tithonia diversifolia leaves demonstrates strong antibacterial activity against Staphylococcus aureus*, with effectiveness increasing proportionally with concentration.
AN OVERVIEW OF ADVANCED 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ANALYSIS TECHNIQUES Ratnawaty, Gervacia Jenny; Indrawati, Ratih; Wahdaniah, Wahdaniah; Rakhman, Khusna Arif
Walisongo Journal of Chemistry Vol. 8 No. 2 (2025): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v8i2.26028

Abstract

Antioxidants play a crucial role in preventing oxidative damage, necessitating reliable analytical techniques for their evaluation. Oxidative stress, resulting from an imbalance between free radicals and antioxidants in the body, has been associated with various chronic diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. Therefore, accurately assessing antioxidant activity is essential for developing health-related products and validating their efficacy. This study reviewed and compared various analytical methods used to determine antioxidant activity, emphasizing their advantages, limitations, and applicability across different sample types. Traditional spectrophotometric assays, such as the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, remain widely used because of their simplicity and cost-effectiveness, although they often suffer from matrix interferences. Advanced techniques, including gas chromatography–mass spectrometry (GC–MS), high-performance liquid chromatography coupled with DPPH (HPLCDPPH), electrochemical methods, microfluidic systems, and miniaturized paper-based assays, provide improved sensitivity, accuracy, and efficiency. These methods enable a more comprehensive assessment of antioxidant activity by offering deeper insights into reaction mechanisms and compound interactions. The integration of multiple analytical approaches can further enhance antioxidant characterization, supporting applications in the food, pharmaceutical, and biomedical industries. This review highlights the importance of selecting appropriate analytical techniques based on research objectives and sample characteristics, while also outlining future directions for advancing antioxidant detection methodologies.
Co-Authors Ari Nuswantoro Ari Widiyantoro Auliannisa, Riska D.M.T, Immaculata Dian Kartika Ningrum Dj, Herlinda Djohan Djohan, Herlinda Emilda Sari Erfiana, Desti Fadli Sukandiarsyah Hendra Budi Sungkawa Hendra Budi Sungkawa Hendra Budi Sungkawa, Hendra Budi Ihsan, Bagus Muhammad Iis Kurniati Iis Kurniati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Indrawati, Ratih Jajar P. Syari Julyani Yuanti Khusna Arif Rakhman, Khusna Arif Laila Kamila Lia, Sestriana Linda Triana Linda Triana Maulidiyah Salim Muhammad Ihsan, Bagus Nelvi Kurnia Sari Nurhayati, Etiek Sari, Emilda Sestriana Lia Sugito - Supardi, Rizal Sutriswanto, Sutriswanto Syari, Jajar Pramata Tumpuk, Sri Wahdaniah Wahyudi Yuanti, Julyani