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All Journal Media Kedokteran Hewan Airlangga Development Journal Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology Jurnal Kedokteran Hewan Frontiers in Community Service and Empowerment
Sri Pantja Madyawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Electrical & Electronics Engineering Engineering Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Combination of 5% Dextrose Ringer's solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature Ahmad Hanif Azzam; Dadik Rahardjo; Tatik Hernawati; Sri Pantja Madyawati; Budi Utomo
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.109-114

Abstract

This study aims to determine the motility and viability of kampung rooster (Gallus domesticus) spermatozoa in 5% Dextrose Ringer's solution as an extender with or without the addition of various egg yolk concentrations at 5ºC during storage. Semen was collected by massage in the morning. In this study, four types of extenders were used, i.e., 5% Dextrose Ringer's extender without egg yolk, 5% Dextrose Ringer's extender with 5, 7.5, and 10% egg yolk for T0, T1, T2, and T3, respectively. Then, 1000 IU Penicillin and 1 mg Streptomycin were added per mL extender. Fresh semen from each rooster was evenly divided into four volumes to be diluted 11 times in each group extender. Extended semen from all groups was stored at 5°C. Data were analyzed using ANOVA and Duncan's new multiple range test. The result showed a significant difference (p <0.05) between each treatment group for spermatozoa motility and viability. In conclusion, this study revealed that the addition of various concentrations of egg yolk into 5% Dextrose Ringer's solution as an extender maintained the motility and viability of kampung rooster spermatozoa.
Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke Maryana Siska Silviani; Moh. Sukmanadi; Rochmah Kurnijasanti; Sri Pantja Madyawati; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.130-136

Abstract

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.
Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

Abstract

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p  <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.
EMPOWERMENT OF ELDERLY POSYANDU VOLUNTERS IN EARLY DETECTION OF HYPERTENSION DISEASE Hariyono, Hariyono; Sri Pantja Madyawati; Syifa Fauziyah; Candra Panji Asmoro; Teguh Hari Sucipto; Leo Yosdimyati Romli; Risanti Handayani
Frontiers in Community Service and Empowerment Vol. 2 No. 4 (2023): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v2i4.53

Abstract

The number of elderly populations in various countries continues to increase along with increasing life expectancy, so it is important to pay special attention to the health of the elderly, including early detection of hypertension. Posyandu volunters have good access to the elderly population because they are actively involved in community health activities. The aim of empowering elderly Posyandu volunters is to increase volunters' knowledge and skills in early detection of hypertension in the elderly. With adequate knowledge, volunters can provide better care and prevent more severe complications. The results of this research were that more than half had poor knowledge about early detection of hypertension before being given training (56%) and most of them had good knowledge about early detection of hypertension after being given training (63%). Empowering elderly Posyandu volunters has significant benefits. Skilled volunters can help increase elderly awareness of the importance of early detection of hypertension. Apart from that, volunters can provide assistance in regular health monitoring so that elderly people can take preventive measures in a timely manner..
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
The effect of epigallocatechin 3-gallate (EGCG) on Sertoli and Leydig cells counts and spermatozoa quality of rats (Rattus norvegicus) exposed to monosodium glutamate Indira, Sitti Az-Zahra; A Ashiila Tsabita; susilowati, Suherni; Sri Pantja Madyawati; Yudaniayanti, Ira Sari; kusnoto, kusnoto; Legowo, Djoko; Hidajati, Nove; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.56-64

Abstract

This study aimed to determine the preventive effect of epigallocatechin 3-gallate (EGCG) on the number of Sertoli and Leydig cells in white rats (Rattus norvegicus) exposed to monosodium glutamate (MSG). This study used 25 male rats which were divided into five groups. The C– group was given only 1% Na-CMC, while the C+ group received 1% Na-CMC and 120 mg/kg BW of MSG. The T1, T2, and T3 groups were administered EGCG at doses of 4, 8, and 16 mg/kg BW, respectively, along with 120 mg/kg BW of MSG. All treatments were administered orally for 28 days. After the treatment period, the rats were sacrificed on day 29. Their testes were collected and processed into histological specimens using hematoxylin eosin (H&E) staining. The results showed that MSG exposure significantly decreased (p <0.05) the number of Sertoli and Leydig cells in the C+ group compared to the C– group. In contrast, the T1, T2, and T3 groups showed increased cell counts following EGCG administration. Epigalocathecin-3-gallate at a dose of 8 mg/kg BW showed the best potential in maintaining spermatozoa motility, viability, and plasma membrane integrity in rats exposed to MSG. EGCG, as an antioxidant, can reduce lipid peroxidation in the spermatozoa plasma membrane, thereby improving spermatozoa viability, motility, and plasma membrane integrity. In conclusion, the administration of EGCG mitigates the harmful effects of MSG on Sertoli and Leydig cell counts in white rats, with an optimal dose of 8 mg/kg BW.
Co-Authors A Ashiila Tsabita Abdullah Hasib Aditya Irawan Agung Budianto Achmad, Agung Budianto Ahmad Hanif Azzam Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Anas Bad’rosana Prawindi Badri Munir Sukoco BUDI UTOMO Candra Panji Asmoro, Candra Panji Cindy Ercha Aulia Putri Dadik Rahardjo Dimas Kunto Satrio Djoko Legowo Epy Muhammad Luqman Erick Fajar Subhekti ERMA SAFITRI Fachry Abda El Rahman Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Frida Aulya Arningdiah Gandul Atik Yuliani Hariyono Hariyono Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Ika WAHYUNI Indira, Sitti Az-Zahra Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kevin Mardotillah Kusnoto Kusnoto, Kusnoto Legowo, Djoko Leo Yosdimyati Romli Linda Sutanto Maryana Siska Silviani Moh. Sukmanadi Mufasirin Muhammad Al-Syafiq bin Abdul Halim Muhammad Aulia Rahman Muhammad Ikmal Robih Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nove Hidajati Nuurin Ajrin Karim Pudji Srianto Rahmatullah, Aldin Akbar Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti . Rimayanti R Rimayanti Rimayanti Risanti Handayani Rochmah Kurnijasanti Sayi Datur Rohmah Soeharsono Soeharsono Sri Hidanah Sri Mulyati Suherni Susilowati Sukma, Puspa Permata Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Wahyu Suprayogi Trilas Sardjito Warsito, Sunaryo Hadi Widjiati . Widjiati W Widjiati Widjiati Widodo, Oky Setyo Wurlina, W Yeni Dhamayanti Zahrorona, Amira Halimah