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Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas Arinda octavia; Budi Utomo; Sri Pantja Madyawati; Tri Wahyu Suprayogi; Agus Sunarso; Iwan Sahrial Hamid
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.65-71

This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.

EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Sri Pantja Madyawati; Rimayanti R; Widjiati W; Agung Budianto Achmad
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-α), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF-α dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.

EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Sri Pantja Madyawati; Rimayanti R; Widjiati W; Agung Budianto Achmad
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2774

Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-α), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF-α dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.

Comparison of reproductive performance of Ongole cross and Ongole-Limousin cross in Bekri district, Central Lampung regency, Lampung province, Indonesia Sukma, Puspa Permata; Ismudiono Ismudiono; Sri Mulyati; Pudji Srianto; Sri Pantja Madyawati; Ira Sari Yudaniayanti; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.9-17

This study aims to compare the reproductive performance of the Ongole cross (PO) and Ongole-Limousin cross (Limpo) on smallholder farmer in Bekri district, Central Lampung regency, Lampung province, a Indonesia. This study used 30 PO cows, 30 Limpo F1 (first filial) cows and 30 Limpo F2 cows. Sample cows had at least two parities and had never had a history of reproductive disorders. This study used primary and secondary data related to sample cows. Primary data was obtained from interviews with farmers and secondary data was obtained from the Department of Plantations, Livestock and Fisheries of Central Lampung regency, and inseminator recording. Data were described descriptively and analyzed using a one-way Analysis of Variance in Statistical Product and Service Solution (SPSS) version 23. The result showed that the services per conception of Limpo F2 cows was higher (p <0.05) than for PO and Limpo F1 cows. Days open and calving interval for Limpo cows (F1 and F2) were longer (p <0.05) than for PO cows. Days open and calving interval for Limpo F1 cows were not significantly different (p >0.05) than for Limpo F2 cows. Reproductive performance of PO cows was better compared to Limpo F1 and Limpo F2 cows. However, there was a data discrepancy between reproductive performance and data on rearing systems, feed quality, and signs of estrus observed by farmers. It could be concluded that PO cows had better reproductive performance than Limpo cows.

Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.

Uterine prolapse and related factors in beef cow at the Besuki Animal Health Center, Situbondo regency, East Java, Indonesia Kevin Mardotillah; Suzanita Utama; Erma Safitri; Herry Agoes Hermadi; Sri Pantja Madyawati; Djoko Legowo; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.146-152

This study aims to determine the factors related to uterine prolapse in beef cow in the Besuki Animal Health Center working area in Situbondo regency. The sample consisted of 100 cows that calved in 2020-2022, whose data was obtained from the Department of Animal Husbandry of Situbondo regency, East Java, Indonesia. Determination of the months of the rainy and dry season was obtained from the Meteorological, Climatological and Geophysical Agency, Juanda Class I Meteorological Station. Other data regarding breed, age, parity of cow and dystocia incidence in cow was obtained by conducting interviews with farmers followed by confirmation with inseminator records, and direct observation of the housing system. The collected data were cross-tabulated and analyzed using Chi-square analysis to determine possible factors causing uterine prolapse. The results showed that the incidence of uterine prolapse in beef cow in the working area was 35% (35/100). The factors of housing, age, parity, and incidence of dystocia were significantly related (p <0.05) to the incidence of uterine prolapse, while breed and season did not have a significant influence (p > 0.05). It could be concluded that the age and parity of the cow at the time of calving, the practice of housing cow, and the incidence of dystocia were related to the incidence of uterine prolapse in cows in the Besuki Animal Health Center operational area.

TEACHER COMPETENCY DEVELOPMENT WITH CSCT MODEL AND PISA FRAMEWORK INTEGRATION Linda Sutanto; Badri Munir Sukoco; Sri Pantja Madyawati; Erick Fajar Subhekti
Airlangga Development Journal Vol. 9 No. 1 (2025): AIRLANGGA DEVELOPMENT JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/adj.v9i1.67876

The Noble Teacher Program enhances teacher competency in Indonesia through the CSCT Model and PISA Framework. It addresses training gaps by creating a sustainable framework aligned with global standards. The program began with Focus Group Discussions (FGDs) involving 20 education experts to identify needs and design a training model. Subsequently, 50 high school teachers in Surabaya participated in workshops and mentoring to improve pedagogical skills, critical thinking, social-emotional competencies, and technology integration. Evaluations showed significant improvements in teacher skills. Formative assessments identified program strengths and improvement areas, while pre-test and post-test comparisons confirmed gains in pedagogical and technological competencies, positively impacting classroom practices. The study concludes that the Noble Teacher Program effectively leverages community engagement to enhance education quality, aligning teacher competencies with global benchmarks. It provides a replicable model for integrating sustainability and global standards into teacher training initiatives.

Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung Zahrorona, Amira Halimah; Sri Pantja Madyawati; Pudji Srianto; Hermin Ratnani; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.46-51

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F2α (PGF2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.

Factors associated with repeat breeding in Holstein Friesian cows at the Tani Wilis Village Cooperative Unit, Sendang District, Tulungagung Regency, Indonesia Dimas Kunto Satrio; M’ Izi Kumala Lazuardi Sultoni; Tasya Apritalia Putri; Soeharsono Soeharsono; Dadik Rahardjo; Djoko Legowo; Gandul Atik Yuliani; Iwan Sahrial Hamid; Mohammad Anam Al-Arif; Sunaryo Hadi Warsito; Pudji Srianto; Sri Pantja Madyawati; Tita Damayanti Lestari; Wurlina Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.67-75

This study aimed to evaluate the association of factors such as nutrition, thermal humidity index (THI), age, and lactation period with the occurrence of repeat breeding of dairy cows. The sample consisted of Holstein Friesian cows aged 1-11 years, with lactation periods ranging from 1 to 7 months, currently in lactation, and experiencing repeat breeding (inseminated three or more times without conception, despite exhibiting normal estrous cycles and intervals). The results showed that feeding practices by farmers did not significantly differ (p >0.05) in relation to the incidence of repeat breeding. THI values recorded in several barns also showed no significant differences (p >0.05), with the cows experiencing light to moderate heat stress. Similarly, the frequency of artificial insemination and the estrous cycle intervals remained within the normal range. The highest incidence of repeat breeding was observed in cows aged 2.5 to 5.5 years, accounting for 78.13%, and during lactation periods of 1-3 months, accounting for 81.26%. It can be concluded that there were no significant differences in the provision of forage and leguminous feed between normal cows and those with repeat breeding, and the crude protein content of forage did not influence its occurrence. Additionally, there was no indirect relationship between THI and repeat breeding. However, the incidence of repeat breeding tended to increase in cows aged 3-4 years, and during the second and third lactation periods.

Prevalence and Risk Factor of Repeat Breeder Syndrome in Dairy Cows in KUD Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province Frida Aulya Arningdiah; Rahmatullah, Aldin Akbar; Cindy Ercha Aulia Putri; Mufasirin; Hermin Ratnani; Rimayanti; Sri Pantja Madyawati; Tita Damayanti Lestari; Muhammad Al-Syafiq bin Abdul Halim; Nuurin Ajrin Karim
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.232-242

This study aims to contribute valuable insights to readers and serve as a reference for dairy farmers looking to enhance their operations. By examining the prevalence and risk factors associated with repeat breeder syndrome in dairy cows within the Village Unit Cooperation (KUD) Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province, this study aims to aid in preventing such occurrences and ultimately improve dairy cow productivity. The survey method was employed, utilizing data sourced from cooperative and farmer records, as well as observations, interviews, and questionnaire responses. Analysis revealed that out of 50 farmers owning a total of 281 cows, 30 farmers possessed a combined total of 58 dairy cows classified as repeat breeders. Consequently, the prevalence rate of repeat breeders among farmers was found to be 20.64%. Descriptive analysis was conducted on the collected data, with additional SPSS Chi-Square (X²) analysis performed to identify risk factors associated with repeat breeders. These risk factors include breeder experience, knowledge of reproduction and estrus phases, communication with artificial inseminators and veterinarians, housing conditions, as well as factors related to livestock feed and water.

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