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ISOLATION PROCESS OF ACID-PRODUCING BACTERIA WITH MODIFIED MEDIA TO OBTAIN ACETOBACTER ACETI IN CASSAVA (MANIHOT UTILISSIMA) FERMENTATION Zaki, Siti Muzakiyah; Syauqi, Ahmad; Ramadhan, Majida
J-PEN Borneo : Jurnal Ilmu Pertanian Vol 6, No 2 (2023)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS BORNEO TARAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/jpen.v6i2.3664

Abstract

Cassava (Manihot utilissima) is a type of high-carbohydrate plant that grows a lot in Indonesia. One of the most common and simple cassava processing is made into tape. One of the microorganisms in yeast is the genus Saccharomyces. Some species of this genus are considered very important in food production materials. One example is Saccharomyces cerevisiae, which is used in winemaking, bread, and beer. This study aims to: study the cassava fermentation process to obtain alcohol compounds as decomposition materials for acid-forming bacteria, knowing the characteristics of acid-forming bacteria in cassava tape fermentation, and getting isolates of Acetobacter aceti bacteria producing acetic acid in the cassava tape fermentation process. This research uses a qualitative descriptive method, with macroscopic, microscopic observations and gram painting on isolates of acid-forming bacteria that have been selected and successfully isolated from cassava fermentation. The results obtained by the colonies showed clear zones and did not show clear zones. Colonies that show clear zones are colonies that produce acid and react with carbonate ions; in the gram test obtained, a genus of bacteria obtained based on the results of isolation on cassava tape, namely some belonging to the genus Acetobacter with gram-negative (colored red) and some genus Lactobacillus with gram-positive (colored purple). The presence of a sour taste on the tape is caused by acid-forming bacteria after the presence of alcohol by the purples Saccharomyces cerevisiae.
Perbandingan Pertumbuhan Saccharomyces cerevisiae dengan Kandungan Hidrogen Peroksida Dalam Substrat Berbasis Gula Serat Diperkaya Sari Stroberi: Comparison of Saccharomyces cerevisiae Growth with Hydrogen Peroxide Content in Strawberry Juice Enriched Fiber Sugar Based Substrate Tresna, Adira Tatus; Syauqi, Ahmad; Ramadhan, Majida
Jurnal SAINS ALAMI (Known Nature) Vol. 7 No. 2 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v7i2.18814

Abstract

The growth of Saccharomyces cerevisiae involves several factors that affect the survival and reproduction of the microorganism, one of which is the carbon substrate that is key to its growth. Fibre sugar-based substrates are glucose-containing materials with residual peroxide. They are often used as a source of nutrients in various biotechnological processes, particularly in bioenergy production, and can be used to support the growth of Saccharomyces cerevisiae. Strawberries (Fragaria sp.) are red due to their rich anthocyanin colour pigment, high in antioxidants and vitamin C. Antioxidants are considered very effective in reducing the action of oxidizers such as hydrogen peroxide (H2O2). This study aims to determine the effect of strawberry juice concentration on the growth of Saccharomyces cerevisiae with peroxide content in the medium. The research method used the randomized block design method, with variations in the concentration of strawberry juice 0%, 5%, 10%, 15%, and 20%, with 7x replicates for 7 days within a period of every 24 hours. The results showed that the content of strawberry juice is needed to increase growth. The 10% concentration had the same growth increase as the 0% extract concentration, but the number of cells was lower at 10,719,205 compared to 21,108,805. There is an effect of strawberry juice antioxidant substances on the growth of Saccharomyces cerevisiae fungal cells at a peroxide concentration of 1000 ppm in the medium.  Keywords: Saccharomyces cerevisiae, Hydrogen peroxide, strawberry, turbidimeter Pertumbuhan Saccharomyces cerevisiae melibatkan beberapa faktor yang mempengaruhi kelangsungan hidup dan reproduksi mikroorganisme tersebut, salah satunya yaitu substrat karbon yang menjadi kunci untuk pertumbuhannya. Substrat berbasis gula serat adalah bahan yang mengandung glukosa dengan residu peroksida dan sering kali digunakan sebagai sumber nutrisi dalam berbagai proses bioteknologi, khususnya dalam produksi bioenergi. Substrat berbasis gula serat dapat digunakan untuk mendukung pertumbuhan Saccharomyces cerevisiae. Buah Stroberi (Fragaria sp.) yang berwarna merah disebabkan kaya akan pigmen warna antosianin, tinggi antioksidan, dan vitamin C. Antioksidan dianggap sangat efektif menurunkan kerja oksidator seperti hidrogen peroksida (H2O2). Penelitian ini bertujuan untuk mengetahui pengaruh kosentrasi sari stroberi terhadap pertumbuhan Saccharomyces cerevisiae dengan kandungan peroksida dalam medium. Metode penelitian menggunakan metode Rancangan Acak Kelompok, dengan variasi konsentrasi sari stroberi 0%, 5%, 10%, 15%, dan 20%, dengan 7x ulangan selama 7 hari dalam kurun waktu tiap 24 jam. Hasil penelitian didapatkan kandungan sari stroberi dibutuhkan untuk memperoleh peningkatan pertumbuhan. Konsentrasi 10% terjadi peningkatan pertumbuhan yang sama seperti konsentrasi ekstrak 0%, tetapi jumlah sel lebih rendah yaitu 10.719.205 dibanding 21.108.805. Adanya pengaruh zat antioksidan sari stroberi terhadap pertumbuhan sel jamur Saccharomyces cerevisiae pada konsentrasi peroksida 1000 ppm dalam medium.    Kata kunci: Saccharomyces cerevisiae, Hidrogen peroksida, stroberi, turbidimeter
Waktu Fermentasi Asam Asetat oleh Isolat Acetobacter aceti dalam Substrat Produk Residu Gracilaria sp: Acetic Acid Fermentation Time by Acetobacter aceti Isolate in Gracilaria sp Residual Product Substrate Ilfathoniyah, Aulia; Ahmad Syauqi; Ramadhan, Majida
Jurnal SAINS ALAMI (Known Nature) Vol. 7 No. 2 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v7i2.18824

Abstract

Utilizing macroalgae plant waste is maximized to reduce the negative impact on the environment. The crude fibre content in Gracilaria sp species as waste from the sortation results of the industrial segment needs can be increased the its value. Environmental conservation form for Gracilaria sp waste is usefull as organic matter and processing needs namely substrate in fermentative activity. This study aims to determine whether plant residues from macroalgae waste Gracilaria sp can be processed into acetic acid and to study variations in the duration of acetic acid fermentation in substrates from plant residual products using Acetobacter aceti cell isolates. This study used a quantitative descriptive method to measure the results of the fermentation process of the acetic acid content. The fermentation process was performed at different time variations of 7.10 and 13 days. Acetobacter aceti inoculum concentration used in this study was 9%. The chemical analysis for determining alcohol content and sugar using UV-Vis spectrophotometry technique, acetic acid with the titration method, and pH testing. The residual product of macroalgae Gracilaria sp waste can be processed into acetic acid and ethanol fermentation using Saccharomyces cerevisia yeast. The yield from the fermentation of Acetobacter aceti cells on day 13 had an acetic acid content of 5.11%, alcohol of 0.71% and sugar of 0.056%. Keywords: Acetic Acid, Fermentation, Plant Residues. Pemanfaatan limbah tumbuhan jenis makro alga diupayakan maksimal untuk mengurangi pengaruh buruk bagi lingkungan. Kandungan serat kasar spesies Gracilaria sp dari limbah hasil sortir kebutuhan pada segmen industri dapat ditingkatkan nilainya.  Bentuk konservasi lingkungan untuk limbah Gracilaria sp adalah pemanfaatan sebagai bahan organik dan perlu pengolahan yaitu substrat aktivitas fermentatif. Penelitian ini bertujuan untuk mengetahui residu tumbuhan dari limbah makro alga Gracilaria sp dapat diolah menjadi asam asetat dan mempelajari lama variasi waktu lama fermentasi asam asetat dalam substrat produk residu tumbuhan dengan menggunakan isolat sel bakteri Acetobacter aceti. Penelitian ini menggunakan metode deskriptif kuantitatif untuk mengukur hasil proses fermentasi yaitu kadar asam asetat. Proses fermentasi dilakukan dengan variasi waktu yang berbeda yaitu 7,10 dan 13 hari. Konsentrasi inokulum Acetobacter aceti yang digunakan dalam penelitian ini adalah 9%. Analisis kimia menggunakan metode spektrofotometri UV-Vis untuk etanol dan gula, penentuan asam asetat dengan metode titrasi, dan uji pH. Produk residu limbah makro alga Gracilaria sp dapat diolah menjadi asam asetat dengan tahapan fermentasi etanol menggunakan ragi roti Saccharomyces cerevisia. Kadar asam asetat hasil fermentasi sel A aceti pada hari ke 13 mempunyai kadar asam asetat 5,11% alkohol 0,71% gula 0,056% . Kata kunci: Asam asetat, Fermentasi, Residu Tumbuhan
Exploration of Secondary Metabolites in Black Pomegranate Peel Extract (Punica granatum L.) as Antioxidants Using DPPH Method Rofiidah, Anisatul; Innaya, Allinha Yusfin; Putri, Nela Vede Rhofa; Maulida, Hilda Jihan; Dewi, Lina Purnama; Al Shofura, Nadya Raisa; Badria, Shoimatul Umi; Faiza, Hilda Wahyu; Hidayah, Ulfa; Ramadhani, Mieta Widya; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 2 (2024): JSMARTech Volume 5, No. 2, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.02.46

Abstract

Pomegranate (Punica granatum) is a plant that contains secondary metabolite compounds that can be used as medicinal content. Black pomegranate contains the highest secondary metabolites compared to red pomegranate and white pomegranate, the skin of the pomegranate contains flavonoids, phenols, tannins, and saponins. This study aims to determine the antioxidant activity of black pomegranate peel extract (Punica granatum L.) with the DPPH method. DPPH method is a method to determine the antioxidant activity in the sample by looking at its ability to counteract the free radical compound 2,2-diphenyl-1-picrylhydrazyl. The results of testing the antioxidant activity of black pomegranate skin (Punica granatum L.) produced an IC50 value of 47.51 ppm which can be concluded that black pomegranate skin is classified as very strong. This research is important because there has not been much research on black pomegranate, especially its skin, so there is a need for additional expansion and renewal of the species. In addition, it can be used as the main basis for the advancement of natural materials, especially in the field of health.
Training on Clean Water Treatment Using Slow Sand Filters And Biomass Filters at Bahrul Magfiroh Islamic Boarding School Malang City Herlina, Sri; Widiyana, Anita Puspa; Ramadhan, Majida; Rahmatullah, Muhammad Ali; Rofiq, Muhammad Fadlan

Publisher : LPPM Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/js.v6i1.4384

Abstract

The purpose of this activity is to increase students' knowledge about PHBS and diarrhea prevention using herbal ingredients as well as the application of clean water treatment simulations using slow sand filter and biomass filter methods. This method of implementing service uses a participatory and collaborative approach through active and interactive discussions. This activity was carried out by involving 54 students of Bahrul Magfiroh Islamic Boarding School Malang. Service participants directly practice and understand the function of applying water treatment devices and the use of herbal ingredients for the prevention of diarrhea. The results of activities on clean water treatment are almost 90% understood by students, both the use of biomass filters and slow sand filters, while 42.6% of students still have a low understanding of how to process herbal ingredients for the prevention of diarrhea. The importance of continuous training on increasing knowledge of clean and healthy living behavior must continue to be improved as an effort to prevent the risk of disease transmission and health problems, including maintaining personal health and the Islamic boarding school environment.
Analisis Aktivitas Antioksidan pada Ekstrak Jagung Muda (Zea mays L.) dengan menggunakan Metode DPPH Ma’rifah, Shofia Jannatul; Wahidah, Shinta Nuriyah; Azizah, Nur; Jamilah, Zahrotul; Wati, Dwi Kartika; Arianti, Leny Ardini; Maulidya, Sabrina Dita; Mudzakaroh, Luluk; Safitri, Dewi Astriana; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Borneo Journal of Biology Education (BJBE) Vol 6, No 2 (2024): Oktober
Publisher : Faculty of Teacher Training and Education Biology Education Departement

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/bjbe.v6i2.5585

Abstract

Pada penelitian ini kami menggunakan jagung muda sebagai pembaharu dari penelitian sebelumnya. Jagung muda merupakan jagung yang masih tergolong mudah (belum berbiji) biasa disebut dengan jagung semi, janggel. Jagung memiliki kandungan fenolik, flavonoid dan tannin yang berguna sebagai antioksidan, yang mampu menghambat dan melindungi radikal bebas. Penelitian ini dilakukan untuk menguji aktivitas ektrak jagung muda menggunakan metode DPPH dengan pelarut etanol. Untuk mengetahui aktivitas antioksidan suatu tanaman, salah satu pengukuran yang paling umum dengan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil antioksidan pada ekstrak jagung muda (Zea mays L.) menggunakan metode DPPH dengan spektrofotometri UV-Vis menunjukkan bahwa nilai IC50 pada jagung muda (Zea mays L.) sebesar 257,92. Sehingga dapat disimpulkan bahwa aktivitas antioksidan ekstrak jagung muda (Zea mays L.) termasuk kategori sangat lemah.
Antioxidant Activity Test On Ethanol Extract Of Cassava Leaves (Manihot Esculenta C.) Using DPPH Method Husain, Muhammad Hafizh; Febrianti, Dewi Retno; Salsabillah, Salma; Rolita, Ninda Norma; Amrullah, Kammal Nizam; Bawani, Elen Ayu; Ramadhani, Lailatul; Pratiwi, Lidia Wahyu; Yaqin, Ngainul; Jayanti, Angelina; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Biopedagogia Vol 6, No 2 (2024): November
Publisher : Program Studi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/biopedagogia.v6i2.5533

Abstract

Cassava leaves (Manihot esculenta Crantz) are herbs used in traditional medicine. Cassava leaves contain secondary metabolite chemicals such as vitamin C, flavonoids, tannins, saponins, and triterpenes. Cassava leaves are pharmacologically active as anti-inflammatory, antibacterial, and antioxidants. To identify the sample's active antioxidant components using the DPPH technique. The DPPH method is a method of determining the antioxidant activity of a sample by examining its capacity to neutralize free radicals of the 2,2-diphenyl-1-picrylhydrazyl molecule. The antioxidant activity of quercetin from cassava leaf extract (Manihot esculenta Crantz) yielded an IC50 value of 210.33. The IC50 value shows that cassava leaves (Manihot esculenta Crantz) have modest antioxidant lower. Cassava leaves (Manihot esculenta Crantz) have antioxidant activity of 210.33 g/mL so they are included in the weak category in this study.
Rancang Bangun Sistem Informasi Berbasis Website untuk BUMDes sebagai Sarana Informasi bagi Masyarakat Ramadhan, Majida; Prasetyo, Doni
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 5, No 1 (2025)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.01.91

Abstract

Badan Usaha Milik Desa (BUMDes) di Desa Ngroto Kecamatan Pujon Kabupaten Malang memiliki peran strategis dalam perekonomian desa yang bertujuan untuk meningkatkan kesejahteraan masyarakat melalui pengelolaan sumber daya yang ada secara profesional dan berlandaskan pada prinsip kerakyatan. BUMDes di Desa Ngroto berfungsi sebagai entitas bisnis yang tidak hanya mengelola berbagai potensi ekonomi lokal tetapi juga berperan dalam pemberdayaan masyarakat dan pengembangan infrastruktur desa. Dengan mengedepankan prinsip kerakyatan, BUMDes ini diharapkan mampu memberikan manfaat langsung kepada warga desa melalui peningkatan lapangan kerja, pengelolaan keuangan yang transparan, serta pengembangan usaha-usaha lokal yang dapat mendorong kemandirian ekonomi desa. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk mengkaji pengelolaan BUMDes yang ada di Desa Ngroto dengan berfokus pada efektivitasnya dalam menjalankan prinsip kerakyatan dan kontribusinya terhadap peningkatan kesejahteraan masyarakat setempat. Kegiatan penyuluhan dan pelatihan penggunaan website BUMDes di Desa Ngroto berhasil meningkatkan pemahaman dan keterampilan teknis para pengelola BUMDes terkait pentingnya digitalisasi dalam manajemen usaha desa. Dengan adanya kegiatan pengabdian kepada masyarakat ini, BUMDes mampu menjadi model tujuan untuk pengelolaan bisnis berbasis masyarakat yang berkelanjutan dan dapat menjadi contoh penerapan model bagi desa-desa lain di Kabupaten Malang. Selain itu, BUMDes juga dapat membantu untuk mengembangkan bisnis masyarakat dalam hal teknologi dan informasi.
Potential Combination of Cermai (Phyllanthus acidus) and Mulberry (Morus alba) Fruit Extract as a Candidate For Tyrosinase Inhibitor Susanti, Erna; AS, Nour Athiroh; Ramadhan, Majida; Mardhiyah, Mardhiyah; Noor, Mahanem Mat
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.42571

Abstract

Melanin, a pigment derived from UV radiation, is crucial in preventing skin damage and can cause aesthetic and dermatological problems such as hyperpigmentation or hypopigmentation. Melanogenesis is a complex process involving enzymes and cytokines, with UV being a primary contributor. Tyrosinase is a key enzyme in melanin synthesis. This study aims to test the potential of combining cermai fruit extract (CE) and mulberry fruit (ME) as a tyrosinase inhibitor. The tests included antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, total phenolic content using the Folin Ciocalteu method, flavonoid content using the AlCl3 colorimetric method, and sun protection factor (SPF) value using UV-Vis spectrophotometric method. The results showed that the highest total phenolic content was observed in (CE), followed by the combination of ME: CE in the ratio of 1:3, 1:1, 3:1, and then ME. The same pattern was seen in the flavonoid content assay results. The antioxidant activity, as indicated by the IC50 values, followed the following order CE 418.30%; ME: CE (1:3) 400.49%; ME: CE (1:1) 367.73%; ME: CE (3:1) 358.04%; and ME 344.43%. The highest SPF value was observed in ME. It can be concluded that this study shows that the combination of CE and ME extracts has potential as a tyrosinase inhibitor and skin protective agent from hyperpigmentation due to UV exposure.
Optimization of Analysis Method on Total Phenol Content and Antioxidant Activity in Peel and Seeds of White Pomegranate (Punica Granatum L.) Faisal; Ramadhan, Majida; Rafsanjani, Ramizard; Januarista, Terra
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.05

Abstract

Pomegranate (Punica granatum L.) peel and seeds are rich in bioactive compounds, particularly phenolics, which contribute significantly to their antioxidant properties. This study aimed to determine the total phenolic content (TPC) and antioxidant activity of white pomegranate peel and seed extracts. TPC was measured using theFolin–Ciocalteu method, while antioxidant activity was assessed via the DPPH radical scavenging assay. Maceration extraction was employed using a 1:1 ethanol–distilled water solvent system. The TPC values of the peel and seed extracts were 99.909 µg/g and 3.424 µg/g samples, respectively, indicating a substantially higher phenolic concentration in the peel. Antioxidant activity, expressed as IC50, was 17.361 ppm for the peel and 26.378 ppm for the seeds, categorizing both as possessing very strong antioxidant potential. The correlation between phenolic content and antioxidant capacity reinforces the role of phenolics as key contributors to antioxidant activity. These results underscore the importance of selecting optimal extraction methods to enhance bioactive compound recovery. The strong antioxidant activity of both peel and seed extracts highlights their potential application as natural antioxidants in the food and pharmaceutical industries.
Co-Authors abdul wachid, abdul Achyar, Zhahrotun Khofifah Nur Ahmad Syauqi ahmad syauqi, ahmad Al Shofura, Nadya Raisa Aldina, Devana Rahma Amrullah, Kammal Nizam Anggraeni, Agintha Silvya Anita Puspa Widiyana Arianti, Leny Ardini Aziz, Achmad Qomarul Aziza, Nur Badria, Shoimatul Umi Bawani, Elen Ayu Dewi, Lina Purnama Eka Fitriah, Eka Erna Sulistyowati Erna Susanti Faisal Faisal Faisal Faiza, Hilda Wahyu Fatmawati Fatmawati Febrianti, Dewi Retno Fitirah, Eka Gemilang, Satria Hidayah, Ulfa Husain Latuconsina Husain, Muhammad Hafizh Ilfathoniyah, Aulia Innaya, Allinha Yusfin Jamilah, Zahrotul Januarista, Terra Jayanti, Angelina Khuluqiyyah, Wildhatu Dzati Fiqhul Mahanem Mat Noor, Mahanem Mat Mahyantika, Seftya Mahyantika, Seftya Putri Mardhiyah, Mardhiyah Maulida, Hilda Jihan Maulidya, Sabrina Dita Ma’rifah, Shofia Jannatul Mudzakaroh, Luluk Muhammad Fahmi Hidayatullah Muzayyanah, Nana Nafisa, Nafisa Nirwana, Linggar Nour Athiroh Abdoes Sjakoer Nur Azizah Nurul Jadid Mubarokati Paramitha, Armanda Bella Prasetyo, Doni Pratiwi, Lidia Wahyu Putri, Nela Vede Rhofa Rafsanjani, Ramizard Rahmatullah, Muhammad Ali Rahmawati, Rizka Dwi Ramadhani, Lailatul Ramadhani, Mieta Widya Ratna Djuniwati Lisminingsih Rofiidah, Anisatul Rofiq, Muhammad Fadlan Rolita, Ninda Norma Safitri, Dewi Astriana Salamah, Forrela Zahwa Salsabillah, Salma Savana, Amartya Gesit Siti Aminah Sri Wahyuni Tresna, Adira Tatus Wahidah, Shinta Nuriyah Wati, Dwi Kartika Wulandari, Rossa Martha Yaqin, Ngainul Yuana, Hera Yudi Purnomo Zaki, Siti Muzakiyah