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All Journal AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Biotropika Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Journal of Dedicators Community Indonesian Journal of Chemical Analysis (IJCA) Jurnal Ilmiah Fillia Cendekia JSMARTech : Journal of Smart Bioprospecting and Technology J-PEN Borneo : Jurnal Ilmu Pertanian Soeropati: Journal of Community Service Borneo Journal of Biology Education (BJBE) Biopedagogia Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Agrikan: Jurnal Agribisnis Perikanan Jurnal Sains Alami (Known Nature)
Ramadhan, Majida
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Potential Combination of Cermai (Phyllanthus acidus) and Mulberry (Morus alba) Fruit Extract as a Candidate For Tyrosinase Inhibitor Susanti, Erna; AS, Nour Athiroh; Ramadhan, Majida; Mardhiyah, Mardhiyah; Noor, Mahanem Mat
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.42571

Abstract

Melanin, a pigment derived from UV radiation, is crucial in preventing skin damage and can cause aesthetic and dermatological problems such as hyperpigmentation or hypopigmentation. Melanogenesis is a complex process involving enzymes and cytokines, with UV being a primary contributor. Tyrosinase is a key enzyme in melanin synthesis. This study aims to test the potential of combining cermai fruit extract (CE) and mulberry fruit (ME) as a tyrosinase inhibitor. The tests included antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, total phenolic content using the Folin Ciocalteu method, flavonoid content using the AlCl3 colorimetric method, and sun protection factor (SPF) value using UV-Vis spectrophotometric method. The results showed that the highest total phenolic content was observed in (CE), followed by the combination of ME: CE in the ratio of 1:3, 1:1, 3:1, and then ME. The same pattern was seen in the flavonoid content assay results. The antioxidant activity, as indicated by the IC50 values, followed the following order CE 418.30%; ME: CE (1:3) 400.49%; ME: CE (1:1) 367.73%; ME: CE (3:1) 358.04%; and ME 344.43%. The highest SPF value was observed in ME. It can be concluded that this study shows that the combination of CE and ME extracts has potential as a tyrosinase inhibitor and skin protective agent from hyperpigmentation due to UV exposure.
Microbial Contaminants Evaluation of White Pomegranate Peel, Seeds, and Beetroot as Validation of Herbal Raw Material Quality Faisal, Faisal; Ramadhan, Majida; Rofiidah, Anisatul; Nirwana, Linggar
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.10

Abstract

Pomegranate (Punica granatum L.) and beetroot (Beta vulgaris) are recognized for their numerous health benefits, attributed to their bioactive. However, the use of natural ingredients as traditional medicine requires attention to microbiological safety, given the potential for contamination by pathogenic microbes. This study aims to analyze microbial contamination on the skin and seeds of white pomegranate (Punica granatum L.) and beetroot (Beta vulgaris). Samples were tested at the Microbiology Laboratory of the Islamic University of Malang using the Total Plate Count (TPC) method to count total microorganisms and qualitative tests to detect the presence of pathogenic microorganisms, such as Salmonella sp. and Staphylococcus aureus.  The results showed that the total microbes and mold/yeast for all samples were within the safe limits set by the Food and Drug Administration (BPOM). The highest TPC was found in white pomegranate peel extract (2,1x10? CFU/g), while the highest Total Yeast and Mold Count (TYMC) was found in white pomegranate seed extract (8,2x10? CFU/g). Although Salmonella sp. was not detected in all samples, Staphylococcus aureus was identified positively in white pomegranate seed extract. These findings underscore that, although the total microbial count is low, contamination by specific pathogens can occur, most likely during post-harvest handling. The presence of Staphylococcus aureus highlights the importance of implementing strict sanitation controls and Good Manufacturing Practices (GMP) in the processing of herbal raw materials to ensure product safety.
Pengaruh Pemberian Zat Pengatur Tumbuh Hormon Auksin dan Sitokinin Terhadap Pertumbuhan Tunas dan Akar Zaitun (Olea europaea) Menggunakan Metode Microcutting Paramitha, Armanda Bella; Achyar, Zhahrotun Khofifah Nur; Azizi, Rosita Imro’atul; Jayanti, Gatra Ervi; Ramadhan, Majida; Prasetyowati, Eka
Biopedagogia Vol 7, No 2 (2025): November
Publisher : Program Studi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/biopedagogia.v7i2.6886

Abstract

Olive plants (Olea europaea) are widely used as traditional medicine and have high economic value, but olive cultivation in Indonesia still faces many challenges. This study aims to evaluate the effect of providing plant growth regulators (ZPT) auxin and cytokinin hormones on the growth of olive shoots and roots using the microcutting method. The study was conducted at the Orchidology Laboratory, Islamic University of Malang with an experimental method of three treatments, namely water control, Biogen, and Bregadium. The parameters observed included the number and quality of shoot and root growth. The results showed that Biogen and Bregadium were able to accelerate the emergence of shoots on the 3rd day, while the control grew on the 10th day. Observations of root growth showed that Bregadium was able to stimulate root formation in more samples even though the quality was still very thin and few. In contrast, Biogen only produced one thin root in one sample. The conclusion of the study states that the combination of Bregadium ZPT hormones has the potential to be more effective in stimulating the growth of olive roots and shoots vegetatively. This study recommends optimizing the concentration and frequency of ZPT application for more optimal growth results.
Phytochemical Characterization and Quantification of Phenolic, Flavonoid, and Tannin Contents in Ethanol Extract of Medicago sativa Ramadhan, Majida; Faisal, Faisal; Nafisa, Nafisa; Mahyantika, Seftya Putri
Indonesian Journal of Chemical Analysis (IJCA) Vol. 9 No. 1 (2026): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol9.iss1.art3

Abstract

Medicago sativa (alfalfa) leaves are known to contain diverse bioactive phytochemicals, particularly phenolic and flavonoid compounds that are commonly associated with antioxidant activity. This study aimed to characterize the phytochemical profile of the ethanol extract of alfalfa leaves and quantify its major phenolic constituents to provide chemical evidence supporting its antioxidant potential. Dried alfalfa leaves were extracted using maceration with ethanol 70%, followed by qualitative phytochemical screening, Fourier Transform Infrared (FTIR) analysis, and spectrophotometric determination of total phenolic, flavonoid, and tannin contents. Phytochemical screening confirmed the presence of alkaloids, phenolics, tannins, flavonoids, and triterpenoids, while saponins were not detected. Quantitative analysis showed that the extract contained a high total flavonoid content (136.25 ± 3.165 mg quercetin equivalents/g extract), along with appreciable levels of total phenolics (19.32 ± 0.814 mg gallic acid equivalents/g extract) and tannins (66.49 ± 0.927 mg tannic acid equivalents/g extract). These findings demonstrate that alfalfa leaf extract is a rich source of polyphenolic compounds, which are widely associated with antioxidant properties, thereby providing a scientific basis for further evaluation of antioxidant activity.
Co-Authors Abdoes Sjakoer, Nour abdul wachid, abdul Achyar, Zhahrotun Khofifah Nur Ahmad Syauqi ahmad syauqi, ahmad Al Shofura, Nadya Raisa Aldina, Devana Rahma Amrullah, Kammal Nizam Amrullah, Kammal Nizam Amrullah Anggraeni, Agintha Silvya Anita Puspa Widiyana Ari Hayati Arianti, Leny Ardini Aziz, Achmad Qomarul Aziza, Nur Azizi, Rosita Imro’atul Badria, Shoimatul Umi Bawani, Elen Ayu Dewi, Lina Purnama Eka Fitriah, Eka Erna Sulistyowati Erna Susanti Faisal Faisal Faisal Faiza, Hilda Wahyu Fatmawati Fatmawati Febrianti, Dewi Retno Fitirah, Eka Gatra Ervi Jayanti Gemilang, Satria Hidayah, Ulfa Husain Latuconsina Husain, Muhammad Hafizh Ilfathoniyah, Aulia Innaya, Allinha Yusfin Jamilah, Zahrotul Januarista, Terra Jayanti, Angelina Khuluqiyyah, Wildhatu Dzati Fiqhul Mahanem Mat Noor, Mahanem Mat Mahyantika, Seftya Mahyantika, Seftya Putri Mardhiyah, Mardhiyah Maulida, Hilda Jihan Maulidya, Sabrina Dita Ma’rifah, Shofia Jannatul Mudzakaroh, Luluk Muhammad Fahmi Hidayatullah Muzayyanah, Nana Nafisa Nafisa, Nafisa Nirwana, Linggar Nour Athiroh Abdoes Sjakoer Nur Azizah Nurul Jadid Mubarokati Paramitha, Armanda Bella Prasetyo, Doni Prasetyowati, Eka Pratiwi, Lidia Wahyu Putri, Nela Vede Rhofa Rafsanjani, Ramizard Rahmatullah, Muhammad Ali Rahmawati, Rizka Dwi Ramadhani, Lailatul Ramadhani, Mieta Widya Ratna Djuniwati Lisminingsih Rofiidah, Anisatul Rofiq, Muhammad Fadlan Rolita, Ninda Norma Safitri, Dewi Astriana Salamah, Forrela Zahwa Salsabillah, Salma Savana, Amartya Gesit Seftya Putri Mahyantika Siti Aminah Sri Wahyuni Tresna, Adira Tatus Wahidah, Shinta Nuriyah Wati, Dwi Kartika Wulandari, Rossa Martha Yaqin, Ngainul Yuana, Hera Yudi Purnomo Zaki, Siti Muzakiyah