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All Journal VALENSI Jurnal Kimia (Journal of Chemistry) Bionatura Indonesian Journal of Applied Sciences Majalah Kedokteran Bandung Jurnal Penelitian Hasil Hutan Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Jurnal Penelitian Hasil Hutan Journal of Nursing Practice and Education Indonesia Journal of Midwifery Sciences Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Jurnal Penelitian Hasil Hutan
Nurlelasari Nurlelasari
Departemen Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Triterpenoids from the Stem Bark of Aglaia cucullata (Meliaceae) and Their Cytotoxic Activity against A549 Lung Cancer Cell Line Desi Harneti; Iqbal Wahyu Mustaqim; Darwati Darwati; Al Arofatus Naini; Purnama Purnama; Erina Hilmayanti; Tri Mayanti; Nurlelasari Nurlelasari; Shabarni Gaffar; Rani Maharani; Kindi Farabi; Unang Supratman; Sofa Fajriah; Mohamad Nurul Azmi; Yoshihito Shiono
Indonesian Journal of Chemistry Vol 23, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.78748

Abstract

The Aglaia species, which contains triterpenoids, is the most numerous in the Meliaceae family. The A. cucullata species, of which there are only a few known examples, has received scant research attention. This investigation aims to identify triterpenoids in an n-hexane preparation of A. cucullata stem bark and evaluate their effects against the A549 lung cancer cell line. Five dammarane-type triterpenoids were isolated from the A. cucullata trunk bark, which is (1) (20S)-20-hydroxydammar-24-en-3-one, (2) cabraleone, (3) cabralealactone, (4) eichlerianic acid, and (5) (+)-fouquierol. Their chemical structures were determined using infrared, high-resolution mass spectrometry, and nuclear magnetic resonance, as well as through data comparison of the reported compounds. Compound 1 was priorly separated from the Aglaia genus, compounds 2–4 were first isolated from the A. cucullata species, and compound 5 has been reportedly isolated from the Meliaceae family and the Aglaia genus. All substances were tested for their lethal potential against the A549 lung cancer cell type. A seco structure in the A ring of dammarane-type triterpenoid might play an important part in the lethal activity of component 4, which showed the greatest activity with an IC50 value of 32.17 µM against the A549 lung cancer cell line.
Limonoids Isolated from Chisocheton pentandrus (Meliaceae) Stembarks and its Cytotoxicity Towards MCF-7 Breast Cancer Cell Line Runadi, Dudi; Anjari, Intan Hawina; Purnama, Purnama; Nurlelasari, Nurlelasari; Harneti, Desi; Mayanti, Tri; Harizon, Harizon; Hidayat, Ace Tatang; Salam, Supriatno; Azmi, Mohamad Nurul; Supratman, Unang
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chisocheton is a large source of limonoids with various pharmacological effects, such as antiviral, antibacterial, antimalarial, antifungal, and cytotoxic activities. This study aimed to isolate, elucidate, and evaluate the cytotoxicity of limonoids from C. pentandrus stembarks. Isolation was carried out using various separation methods including extraction and column chromatography. Spectral data were analyzed by FT-IR, UV, DEPT 135°, 1H, 13C-NMR, and HRTOF-MS and compared with those previously reported to determine the chemical structure.The obtained limonoids were lasiocarpine (1), lasiocarpine B (2), pentandricine (3), and 16β-hydroxydysobinin (4); all of which were successfully isolated from C. pentandrus for the first time. The cytotoxic activity of these limonoids were also evaluated against Michigan Cancer Foundation-7 (MCF-7) breast cancer cells using PrestoBlue method. Lasiocarpine A revealed the strongest cytotoxicity with an IC50 of 42.62 µM in moderate level.
Hubungan Tingkat Pengetahuan Ibu, Jarak ke Pelayanan Kesehatan, serta Peran Tenaga Kesehatan dalam Perilaku Pemberian Imunisasi Difteri, Pertusis, Tetanus (DPT) Nurlelasari, Nurlelasari; Novita, Astrid
Indonesia Journal of Midwifery Sciences Vol. 3 No. 2 (2024): Indonesia Journal of Midwifery Sciences
Publisher : SCIPRO Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53801/ijms.v3i2.163

Abstract

Latar Belakang: Imunisasi DPT penting diberikan kepada anak-anak untuk kesehatan mereka saat ini hingga pada masa mendatang. Namun masih terdapat anak-anak di Indonesia yang belum menerima imunisasi lengkap, bahkan sama sekali tak pernah diimunisasi sejak lahir. Tujuan: Untuk mengetahui hubungan tingkat pengetahuan ibu, jarak ke pelayanan kesehatan, serta peran tenaga kesehatan dengan perilaku pemberian imunisasi DPT di BPM Wati Cahyawati Tahun 2023. Metode: Penelitian ini merupakan jenis kuantitatif yang bersifat analitik. Populasi dalam penelitian ini merupakan orangtua yang memiliki balita diatas 18 bulan pada periode Kunjungan DPT Desember 2022 – Januari 2023 yaitu sebanyak 55 orang. Sampel dalam penelitian ini adalah sebanyak 55 responden dengan teknik pengambilan sampel total sampling. Instrumen penelitian menggunakan kuesioner. Peneliti menggunakan uji Chi-Square dengan tingkat kepercayaan 95% atau tingkat kemaknaan sebesar 5%. Hasil: Berdasarkan hasil diketahui hubungan tingkat pengetahuan ibu dengan perilaku pemberian imunisasi DPT di BPM Wati Cahyawati Tahun 2023 dengan nilai P-value = 0,004. Hubungan jarak ke pelayanan kesehatan dengan perilaku pemberian imunisasi DPT di BPM Wati Cahyawati Tahun 2023 dengan nilai P-value = 0,007. Hubungan peran tenaga kesehatan dengan perilaku pemberian imunisasi DPT di BPM Wati Cahyawati Tahun 2023 dengan nilai P-value = 0,001. Kesimpulan: Ada hubungan tingkat pengetahuan ibu, jarak ke pelayanan kesehatan, serta peran tenaga kesehatan dengan perilaku pemberian imunisasi DPT di BPM Wati Cahyawati Tahun 2023.
Morelloflavone and Molecular Docking from Stembark of Chisocheton lasiocarpus and Its Cytotoxic Activity Against Breast Cancer Mcf-7 Cell Lines Nurlelasari, Nurlelasari; Harneti, Desi; Maharani, Rani; Darwati, Darwati; Mayanti, Tri; Farabi, Kindi; Hanafi, Muhammad; Supratman, Unang
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6845

Abstract

Chisocheton is a plant of the Meliaceae family which has been known as a source of limonoids, triterpenoids, steroids, alkaloids and phenolics. This plant is the second largest in the Meliaceae, with 53 species widely distributed in tropical and subtropical regions including Indonesia. From this genus, compounds that have interesting activities have been found, including anti-inflammatory, antimalarial, cytotoxic, antitumor and anticancer. One of the species that has the potential to find new compounds is Chisocheton lasiocarpus because there are still few phytochemical studies. This study aims to inform the structural elucidation of one of the compounds from the stem bark of C. lasiocarpus, namely morelloflavone and its cytotoxic potential against breast cancer cells using the in silico method against ER-α receptors (PDB code: 3ERD), ER-β (PDB code: 1QKM). ) and HER-2 (GDP code: 3PP0).
Triterpenoids From Swietenia mahagoni L Jacq. and Their Cytotoxic Activity against MCF-7 Breast Cancer and CV-1 Normal Kidney Cell Lines Supratman, Unang; Abdullah, Fajar Fauzi; Farabi, Kindi; Nurlelasari, Nurlelasari; Awang, Khalijah; Nafiah, Mohamad Azlan
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.9358

Abstract

ABSTRACT. Triterpenoid is a group of natural products with various remarkable activities, including cytotoxic. One of the sources for this type of compounds is Swietenia genus belong to Meliaceae family. This genus known to contains various secondary metabolites including limonoids, flavonoids, and triterpenoids. Especially for triterpenoid, there is limited report on isolation as well as biological activity from Swietenia genus, such as Swietenia mahagoni. In this research, three triterpenoid compounds have been successively isolated and identified from n-hexane extract, namely, (-)-leucophyllone (1), toonaciliatavarin E (2), and moronic acid (3). All isolated were assed against MCF-7 breast cancer and CV-1 normal kidney cell lines. The result showed that only moronic acid (3) performed moderate activity against two cells with IC50 of 63.10 and 48.04 µM, respectively. The other isolated compounds (1 and 2) showed weak cytotoxicity with IC50 > 500 µM. Based on preliminary structure activity relationship, revealed that pentacyclic triterpenoid (moronic acid, 3) showed stronger cytotoxicity compare with tetracyclic triterpenoids (-)-leucophyllone (1) and toonaciliatavarin E (2). Keyword: Cytotoxicity, MCF-7 and CV-1, Switennia mahagoni, Triterpenoids.
Sesquiterpenoids from The Stem Bark of Aglaia cucullata (Meliaceae) and Their Cytotoxic Activity Against A549 Lung Cancer Cell Lines Mustaqim, Iqbal Wahyu; Harneti, Desi; Naini, Al Arofatus; Hilmayanti, Erina; Darwati, Darwati; Nurlelasari, Nurlelasari; Mayanti, Tri; Maharani, Rani; Farabi, Kindi; Hidayat, Ace Tatang; Supratman, Unang; Fajriah, Sofa; Azmi, Mohamad Nurul; Shiono, Yoshihito
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.9823

Abstract

ABSTRACT. Sesquiterpenoids are a class of terpenoid compounds with a remarkable diversity of structures and biological activities. Sesquiterpenoids are primarily found in higher plants, such as the Meliaceae family's Aglaia genus. Aglaia cucullata is a species of Aglaia that is still rarely explored and can potentially contain sesquiterpenoid compounds with cytotoxic activity. Hence, the research intended to isolate sesquiterpenoids from the n-hexane extract of A. cucullata stem bark and evaluate their cytotoxic effect against A549 lung cancer cells. Compounds 1 and 2 were isolated and purified from n-hexane extracts utilizing various chromatographic techniques. The structure of compounds 1 and 2 was determined by analyzing various spectroscopic methods (IR, MS, and NMR) and comparing them to previously reported spectral data. Compound 1 was identified as an isodaucane-type sesquiterpenoid, 10-hydroxy-6,10-epoxy-7(14)-isodaucane, and was first reported in Meliaceae family. Compound 2 was confirmed as an eudesmane-type sesquiterpenoid, eudesm-4(15)-ene-1β,6α-dihydroxy, and was first reported in Aglaia cucullata. Cytotoxic activity of 1 and 2 were investigated in vitro against A549 lung cancer cells using the PrestoBlue assay and resulted in inactive and low cytotoxicity with IC50 values of 292.77 and 90.55 μM, respectively. Key words: Aglaia cucullata, , A549 cell lines, cytotoxic activity, Meliaceae, sesquiterpenoids.
Four Azadirone-Type Limonoids from Chisocheton Pentandrus Stem Bark and Their Cytotoxic Acitivity Against Mcf-7 Breast Cancer Cell Lines Supratman, Unang; Runadi, Dudi; Naini, Al Arofatus; Purnama, Purnama; Mayanti, Tri; Harneti, Desi; Nurlelasari, Nurlelasari; Maharani, Rani; Farabi, Kindi; Nafiah, Mohamad Azlan
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.11085

Abstract

ABSTRACT. Limonoid group belongs to triterpenoid that has undergone further oxidation accompanied by the loss of four carbon atoms to form a furan ring in the chain. This limonoid compound is often found in the Chisocheton genus. Limonoid compounds have been known as compounds with high structural variations and this makes limonoids have diverse and interesting activities, including cytotoxic and anticancer. In the course of our continuing study for limonoid constituents that have cytotoxic activity against cancer cell lines, methanol extract of stem bark from the Chisocheton pentandrus plant provides significant activity. The methanol extract was separated using various chromatographic techniques in the normal and reverse stationary phase to produce four azadirone-type limonoid compounds (1-4). The elucidation structure of 1-4 was determined using spectroscopic methods including, UV-Visible, IR, and 1D-NMR as well as optical rotation. All four known compounds were established as trichilenone acetate (1), toonaciliaton C (2), 11α-acetoxiazadiron (3), and 16β-hydroxydisobinine (4). The cytotoxicity of compounds 1-4 was assessed by examination using the resazurin method, which showed that compound 4 was the promising constituent against the MCF-7 cells with an IC50 value of 43.1 µM and was stronger than its positive control. Keywords: Azadirone-type limonoid, Chisocheton pentandrus, cytotoxic activity, Meliaceae.
Dysobinol Extracted from Chisocheton Macrophyllus Triggers Proliferation Inhibition, Potential Apoptosis, and Cell Cycle Arrest of He La Cancer Cell Lines Gaffar, Shabarni; Hafiz, Ersanda; Wiraswati, Hesti Lina; Ishmayana, Safri; Nurlelasari, Nurlelasari
Majalah Kedokteran Bandung Vol 56, No 1 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3249

Abstract

Dysobinol is a new limonoid from C. macrophyllus seeds reported to have an anticancer activity. This study aimed to determine the cytotoxic activity of Dysobinol against HeLa cancer cell lines and evaluate its mechanism of action by determining the expression level of several carcinogenesis genes related to apoptosis and cell cycle. In this experimental study, the cytotoxic activity was determined using the MTS assay and gene expression by real-time reverse transcriptase PCR. The result shows that Dysobinol has an anticancer activity in a dose and time-dependent manner against HeLa cells and was categorized as toxic with IC50 values of 52.92, 52.70, and 14.96 μg/ml for 24, 48, and 72 hours, respectively. Dysobinol significantly increased the expression of Bax, Cas-8, and Cas-3 and decreased the expression of Cyc D1 at both doses (IC50 and 2x IC50) but only high doses (2x IC50) could affect Cas9 and NF-κB expressions, indicating that Dysobinol can induce apoptosis via the extrinsic pathway and inhibits the cell cycle through the Cyc D1 regulator. Dysobinol has the potential to be developed as a chemotherapy drug or an adjuvant agent for cervical cancer treatment.
Sesquiterpenoids from Dysoxylum amooroides Stem Bark: Isolation, Structure Determination, and Cytotoxicity Against MCF-7 Breast Cancer Cells Gunawan, Latifah; Mustofa, Hidayat Nurul; Naini, Al Arofatus; Harneti, Desi; Hidayat, Ace Tatang; Nurlelasari, Nurlelasari; Maharani, Rani; Mayanti, Tri; Fajriah, Sofa; Awang, Khalijah; Azmi, Mohamad Nurul; Supratman, Unang
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.99121

Abstract

Three sesquiterpenoids, guaianediol (1), alismol (2), and spathulenol (3), were isolated from the n-hexane and ethyl acetate extracts of the stem bark of Dysoxylum amooroides. The three compounds were found in D. amooroides species for the first time. The structures of the isolated compounds were identified and established based on an extensive spectroscopic analysis involving HR-TOF-ESI-MS, IR, and NMR data, as well as a comparison with the previously reported works of literature. Compounds 1-3 were further assessed for cytotoxic effects against MCF-7 breast cancer cells. Guaianediol (1) showed inactive activity with IC50 > 100 µM, alismol (2) showed weak activity with IC50 value of 82.1 µM and spathulenol (3) showed considerable activity with an IC50 value of 15.2 µM. A brief structure-activity relationship and comparison with the previous works were also discussed to understand better the role of guaiane- and aromadendrane-type sesquiterpenoids in the biological activity perspective.
ISOLASI ASAM 3β-HIDROKSI-5-GLUTINEN-28-OAT DARI KULIT BATANG ASAM KANDIS (Garcinia cymosa) DAN AKTIVITAS SITOTOKSIKNYA TERHADAP SEL KANKER PAYUDARA MCF-7 Darwati, Darwati; Khairunnisa, Shofiyah; Nurlelasari, Nurlelasari; Herlina, Tati; Supratman, Unang; Hanafi, Muhammad
Jurnal Penelitian Hasil Hutan Vol. 42 No. 1 (2024): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jphh.2024.2890

Abstract

The Garcinia genus comes from the Clusiaceae family which grows a lot in the tropical forests of Indonesia. This genus is reported to contain many secondary metabolites, one of which is triterpenoid which is useful as a cytotoxic, anti-oxidant, anti-inflammatory, anti-HIV, and antibacterial. One species of Garcinia whose triterpenoid content is still not widely known is G. cymosa. This study aims to obtain triterpenoid compounds in G. cymosa stem bark that are active against MCF-7 breast cancer cells. G. cymosa stem bark powder (0.9 kg) was macerated in stages with n-hexane and ethyl acetate as solvents. The ethyl acetate extract showed positive results for containing triterpenoid compounds after being tested with the Liebermann-Burchard reagent, so the ethyl acetate extract (25 g) was purified and purified by various chromatographic methods. The chemical structure of pure isolate (10 mg) was determined using various spectroscopic techniques IR, MS, 1H-NMR, 13C-NMR, 1H-1HCOSY, HMQC, and HMBC and compared with the literature so that it was designated as triterpenoid acid 3β-hydroxy-5- gluten-28-oat and required its cytotoxic activity using the Presto Blue method against MCF-7 breast cancer cells with an IC50 value of 94.78 µg/mL. This IC50 value belongs to the active category so this compound has potential as an anticancer drug.
Co-Authors A. T. Hidayat1 Ace Tatang Hidayat Ace Tatang Hidayat Achmad Zainuddin Agus Safari Agus Safari Agus Safari Al Arofatus Naini Alya Tsamrotul Anastasya Firdausi Andi Rahim Anggit Kartikasari Anjari, Intan Hawina Astrid Novita, Astrid Awang, K. - Azmi, Mohamad Nurul Christina Marpaung D. D. Harneti D. Darneti D. G. Katja D. Harneti D. Sumiarsa Dadan Sumiarsa Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati, Darwati Desi Harnet Desi Harneti Putri Huspa Dessy Yulyani Kurnia Dewa Gede Katja Dewa Gede Katja DUDI RUNADI E. Julaeha Erina Hilmayanti Euis Julaeha Euis Julaeha Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Farabi, Kindi Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Ghina Izdihar Gunawan, Latifah Hafiz, Ersanda Harizon Harizon Harneti, D - Herdanu Rizqullah Hersa Milawati Hersa Milawati Hesti Lina Wiraswati Hilmayanti, Erina Ida Nur Farida Ida Nurfarida Intan Rahmayanti Iqbal Wahyu Mustaqim Iryanto, Y. - J. Sianturi Jamaludin Al-Anshori Julaeha, E. - Julita Nahar, Julita K. K. Farabi K. K. Haikal Kansy Haikal Kansy Haikal Khadijah Awang Khairunnisa, Shofiyah Khalijah Awang Khalijah Awang Khalijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi M. M. Muhammad Mayanti, T. - Moelyono Moektiwardoyo Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Muchlis, Handi Nugraha Muflihah, L.F - Muhammad Hanafi Muhammad Hanafi Mustaqim, Iqbal Wahyu Mustofa, Hidayat Nurul Nadya Thufaila Nafiah, Mohamad Azlan Naini, Al Arofatus Nita Ike Dwi Kurniasih Nur Muhammad Miftah Nurabi Ferdiana Purnama Purnama Purnama Purnama Puspa, H - R. Maharani R. Maharani R. R. Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Ph.D Rizky Abdullah Ronauli Fitriana Russiska Russiska S. Salam S. Soidah Sabarudin, L. - Safari, A - Safri Ishmayana Safri Ishmayana Salam, Supriatno Sari, Aprilia Permata Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar Shiono, Yoshihito Sinaga, Siska Elisahbet Siska Mulya Octavia Siska Mulya Octavia Siti Hani Pratiwi Sofa Fajriah Sofa Fajriah Sri Purwani Subekti Mauluddin Supriatno Salam Supriatno Supriatno T. Mayanti Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tiara Prima Amalya Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti U. Supratman U. U. Supratman Unang Supratman Wardoyo, M.M - Winda Sukmawati Y. P. Apriantini Y. Shiono Y. Y. Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Zulfikar Zulfikar