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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) SAINSMAT bionature Jurnal Agroteknos BioWallacea Journal of Biological Research Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biodjati ALCHEMY Jurnal Penelitian Kimia Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal MediLab Mandala Waluya Jurnal Abdimas Indonesia : Jurnal Abdimas Indonesia Jurnal Abdi Masyarakat Indonesia Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Jurnal Pengabdian Masyarakat Nasional Jurnal Pusat Kolaborasi Pengabdian Kepada Masyarakat Jurnal MediLab Mandala Waluya
Muzuni, Muzuni
Program Studi Bioteknologi FMIPA UHO, Kendari
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Journal : BioWallacea Journal of Biological Research

 1 
KARAKTERISASI FRAGMEN GEN 18S rRNA POKEA (Batissa violacea celebensis Martens, 1897) DI SUNGAI POHARA KECAMATAN SAMPARA KABUPATEN KONAWE (Characterization of 18S rRNA Gene Fragmen from Pokea (Batissa violacea celebensis Martens, 1897) in the Pohara Rive ., Muzuni; Adi, Dwi Arinto; Syarif, Satriani
Jurnal BioWallacea Vol 1, No 1 (2014)
Publisher : Jurnal BioWallacea

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (26.797 KB)

Abstract

This study aims to characterize sequences of 18S rRNA gene fragment from Pokea (Batissa violacea celebensis Martens, 1897) and its role in differentiated Pokea with other Bivalvia. The method used is a series of PCR (Polymerase Chain Reaction) reaction used to determine the sequence of 18S rRNA gene fragments Pokea. Analysis of the data using program NCBI (National Center for Biotechnology Information), Clustal X, Phydit (Phylogenetik editor), and TreeViewX for characterization 18S rRNA gene sequence to construct a phylogenetik tree of Pokea. The results showed that character of 18S rRNA gene fragments Pokea, namely: size 827 bp, including the family Corbicullidae because it has the closest kinship with Corbicula fluminea, as well as having restriction enzyme sites XhoI, PstI, BamHI, and DraI.   Keywords: Morphology, characterization, 18S rRNA gene, Pokea (Batissa Violacea celebensis Martens, 1897).
Karakterisasi Morfologi Phytophthora sp. Asal Buah Kakao Desa Olo-oloho, Kabupaten Konawe, Sulawesi Tenggara Muzuni Muzuni; Haidin Haidin; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 1 (2020): BioWallacea and Sains
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1145.963 KB) | DOI: 10.33772/biowallacea.v7i1.11812

Abstract

This study aimed to determine the morphological characteristics of Phytophthora sp. isolated from cocoa fruits from Olo-oloho Village, Konawe Regency, Southeast Sulawesi. Isolation of Phytophthora sp. carried out by the point method using V4 (Vegetable Juice Agar) media incubated at 27ºC for 24 hours. Morphological characterization of Phytophthora sp. included characterization of colony morphology and cell morphology. The results showed that the colony morphological characteristics were white colonies, cotton-like textures, the uneven edge of the colony, zoning and radial lines. The morphological characteristics of the cell had asexual spores in the form of sporangium and chlamydospores, hyphae are not aseptic, greenish-black zoospores, zoospores are round and double-flagged, and have sporangiophores. Keywords: Phytophthora sp., colony morphology, cell morphology
EKSPLORASI BAKTERI TERMOHALOFILIK POTENSIAL PENGHASIL L-ASPARAGINASE SEBAGAI ANTIKANKER DI SUMBER AIR PANAS WAWOLESEA Jamaluddin Jamaluddin; Alfin Alfin; Muzuni Muzuni; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.59 KB) | DOI: 10.33772/biowallacea.v5i1.4591

Abstract

ABSTRAK Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea. Bakteri termohalofilik penghasil L-asparaginase asal sumber air panas Wawolesea diperoleh dengan tahapan: survey dan pengukuran parameter lingkungan sumber air panas Wawolesea; isolasi bakteri pada media NA (Nutrient Agar) dan seleksi bakteri penghasil enzim L-asparaginase pada media M-9. Hasil isolasi menunjukkan adanya 52 isolat bakteri termohalofilik dan 14 isolat diantaranya mampu menghasilkan L-asparaginase. Kata Kunci: Bakteri Termohalofilik, L-Asparaginase, Sumber Air Panas Wawolesea ABSTRACT  The objective of this study was to obtain isolates of thermohalophilic bacteria producing L-asparaginase enzyme from Wawolesea hot spring. L-asparaginase producing thermohalophilic bacteria from the Wawolesea hot spring are obtained by steps; survey and environmental parameters measurement of Wawolesea hot spring, isolation of bacteria on NA (Nutrient agar) medium and selection of L-asparaginase producing bacteria on M-9 medium. The isolation results showed 52 isolates of thermohalophilic bacteria and 14 isolates of them capable of producing L-asparaginase. Keywords: Thermohalophilic bacteria, L-asparaginase, Wawolesea hot spring
Efektivitas Ekstrak Etanol Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) Sebagai Antipiretik Pada Mencit (Mus muscullus L.) Hiperpireksia Muzuni Muzuni; Uhra Ali; Wa Ode Harlis
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v9i1.25505

Abstract

This study aims to find out the effectiveness of ethanol extract rhizome curcuma (Curcuma xanthorrhiza Roxb.) as an antipyretic in mice(Mus muscullus L.) hyperpireksia. This study is an experimental study with the Complete Randomized Design (RAL) method. A total of 15 male mice weighing 30-35 grams were divided into 5 treatment groups namely KN (drinking water), KP (paracetamol), P1 (extract 3 mg/gr BB), P2 (extract 8 mg/gr BB) and P3 (extract 14 mg/gr BB). Hyperpyreksia is obtained by inducing mice with 10% peptons. When it reaches above normal temperatures (35.50C - 37.00C), it is given extracts according to the treatment group and measured its effect at minutes 30, 60 and 90. The data was analyzed using ANOVA with α=0.05 and advanced tests of LSD. The results showed P3 was more effective and faster at lowering body temperature, compared to KN, P1 and P2. Seen in the 90th minute the body temperature squeaked P3 (35.960C) back to normal temperatures and closer to KP (35,760C), after which followed by P2 (36.30C). KN (38,630C) and P1 (37,630C) are still categorized as high, as they have not returned to normal temperatures. The results can be concluded that the ethanol extract of curcuma rhizome has antipyretic activity. ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol rimpang temulawak(Curcuma xanthorrhiza Roxb.) sebagai antipiretik pada mencitMus muscullus L.) hiperpireksia. Penelitian ini merupakan penelitian eksperimental dengan metode Rancangan Acak Lengkap (RAL). Sebanyak 15 ekor mencit jantan berat 30-35 gram dibagi dalam 5 kelompok perlakuan yaitu KN (air minum), KP (parasetamol), P1 (ekstrak 3 mg/gr BB), P2 (ekstrak 8 mg/gr BB) dan P3 (ekstrak 14 mg/gr BB). Mencit hiperpireksia diperoleh dengan cara menginduksi mencit dengan pepton 10 %. Ketika mencapai di atas suhu normal (35,50C - 37,00C), maka diberikan ekstrak sesuai kelompok perlakuan dan diukur pengaruhnya pada menit ke 30, 60 dan 90. Data dianalisis menggunakan ANOVA dengan α=0,05 dan uji lanjut LSD. Hasil penelitian menunjukkan P3 lebih efektif dan lebih cepat menurunkan suhu tubuh mencit, dibandingkan dengan KN, P1 dan P2. Dilihat pada menit ke-90 suhu tubuh mencit P3 (35,960C) kembali ke suhu normal dan lebih mendekati KP (35,760C), setelah itu diikuti P2 (36,30C). KN (38,630C) dan P1 (37,630C) masih dikategorikan tinggi, karena belum kembali ke suhu normal. Hasil tersebut dapat disimpulkan bahwa ekstrak etanol rimpang temulawak memiliki aktivitas antipiretik.Kata Kunci : Antipiretik, Temulawak, Mencit, Efektitivitas, Hiperpireksia.
Co-Authors , Suharsono . Jahidin . Jahidin, . Abe, Wa Ahmad Mustafa Ahmad, Sitti Wirdhana Alfin Alfin Alias, Alias Amin, Muhtar Amirullah -, Amirullah Ampa, Andi Tendri Andi Septiana Andriani, Rina Ansharullah ansharullah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Aspadiah, Vica Asriwaty, Asriwaty Asrul Sani Azwar Syah, Muhamad Bafadal, Mentarry Balubi, Abdul Muis Barti, Barti Basrudin Basrudin Damhuri Damhuri Didy Sopandie DIRVAMENA BOER, DIRVAMENA Dwi Arinto Adi, Dwi Arinto Effendie, Muh Yamin Fahyuddin Fahyuddin, Fahyuddin Fristiohady, Adryan Hadijah Hadijah Haidin Haidin Halim Halim Halim Halim, Halim Hasani, La Ismaun Ismaun Jahidin Jahidin Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili kaharudin, la ode Kasim, Ma'ruf La Maronta Galib La Maronta Galib, La Maronta MANGIRI, NINDY VANESSA Muhsin Muhsin Muhtar Amin Nur Arfa Yanti Nur Hikmah Nur Ramdani Nur Salam Nursalam Nursalam Nurwijayanti Prasetya, Wandy Murti Puan Maharani Puspita Sari Ramadhani, Rizky Barkah Rinto Rinto Ruslin Hadanu Ruslin Hadanu Safilu Safilu Sahidin . Samsaifil Samsaifil Satriani Syarif Satriani Syarif, Satriani Solly Aryza Sri Ambardini Sri Wahyuni Suharsono . Suriana Suriana Suriana Suriana Tamrin Tamrin Taufik Walhidayah Trisandy, Darul Uhra Ali Utami, Andi Pratiwi Utut Widyastuti Suharsono Wa Ode Harlis Wardha Jalil Warhamni, Warhamni Yusnaini Yusnaini