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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Farmasi Natural Science: Journal of Science and Technology Biocelebes KOVALEN: Jurnal Riset Kimia Pharmascience Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Indonesian Journal of Chemistry Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Jurnal Ilmu Kefarmasian Indonesia Molekul: Jurnal Ilmiah Kimia Moderatio : Jurnal Moderasi Beragama Jurnal Pengabdian Farmasi dan Sains Academic Journal of Islamic Principles and Philosophy Jurnal Muria Pengabdian Masyarakat
Syariful Anam
Jurusan Farmasi, Fakultas MIPA, Universitas Tadulako, Palu, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : Natural Science: Journal of Science and Technology

 1 
AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK KULIT BUAH MENTAH PISANG AMBON (MUSA PARADISIACA VAR.SAPIENTUM) TERHADAP STAPHYLOCOCCUS AUREUS Normayunita, Sri; Anam, Syariful; Khumaidi, Akhmad
Natural Science: Journal of Science and Technology Vol 4, No 3 (2015): Volume 4 Number 3 (December 2015)
Publisher : Univ. Tadulako

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Abstract

Telah diketahui Bahwa ekstrak etanol kulit buah mentah pisang ambon (Musa paradisiaca var.sapientum) mempunyai aktivitas antibakteri terhadap Staphylococcus aureus. Ekstrak kulit buah mentah pisang ambon diperoleh melalui ekstraksi secara maserasi dengan pelarut etanol 96%, dengan fraksinasi menggunakan metode kromatografi cair vakum, fraksi yang diperoleh dilakukan pengujian aktivitas antibakteri dengan metode difusi agar menggunakan paper disc. Penelitian ini bertujuan untuk menentukan fraksi yang dapat menghambat pertumbuhan bakteri Staphylococcus aureus dengan aktivitas tertinggi dan menentukan fraksi yang mempunyai efektivitas hampir sama dengan kontrol positif (tetrasiklin). Data yang diperoleh berupa statistik, dari hasil uji two-way ANOVA menunjukan bahwa secara keseluruhan terdapat perbedaan yang bermakna (p<0,05) terhadap semua jenis sampel. Berdasarkan uji Tukey HSD menunjukan bahwa aktivitas paling tinggi dari keempat fraksi dengan konsentrasi 30, 60, dan 90 µg/µL terdapat pada fraksi dengan konsentrasi 90 µg/µL. Pada kontrol positif untuk semua kelompok uji terdapat perbedaan bermakna (p< 0,05), artinya efektivitas kontrol positif dengan semua perlakuan menunjukan perbedaan efektivitas antara kontrol positif dengan semua fraksi uji. Dapat disimpulkan bahwa fraksi aktif dengan konsentrasi 90 µg/µL miliki aktivitas paling besar dan tidak ada efektivitas fraksi yang hampir sama dengan kontrol positif.
UJI EFEKTIFITAS EKSTRAK DAUN DAN AKAR HARRISONIA PERFORATA MERR.TERHADAP PERTUMBUHAN BAKTERI VIBRIO CHOLERAE Al Amrie, Amir Galeb; Ivan, Ivan; Anam, Syariful; Pitopang, Ramadhanil
Natural Science: Journal of Science and Technology Vol 3, No 3 (2014): Volume 3 Number 3 (December 2014)
Publisher : Univ. Tadulako

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Abstract

Penelitian tentang uji efektifitas ekstrak daun dan akarHarrisonia perforata Merr.terhadap pertumbuhan bakteri Vibrio cholerae telah dilaksanakan pada bulan Maret sampai Mei 2014. Tujuan penelitian ini yaitu untuk mengetahui keefektifan ekstrak daun dan akarH. perforata Merr.dalam menghambat pertumbuhan bakteri V. cholerae. Metode ekstraksi yang digunakan dalam penelitian yaitu metode Maserasi dan Refluks.Sedangkan pengujian daya hambat ekstrak terhadap bakteri V. Cholerae dengan menggunakan metode difusi agar (metode sumur).Penelitian ini disusun dalam Rancang Acak Lengkap (RAL) dengan 7 perlakuan dan 3 kali ulangan (untuk masing-masing daun dan akar). Perlakuan yang dicobakan yaitu pemberian konsentrasi ekstrak (daun maupun akar) 10 %, 20 %, 40 %, 60 % dan 80 % serta antibiotik doxycycline 2 % sebagai kontrol positif dan NA-CMC 1 % sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak 80 % (daun maupun akar) tumbuhan H. perforata Merr.menghasilkan zona hambat yang paling besar dibanding kosentrasi lainnya dengan masing-masing sebesar (±26,53 mm dan±14,72 mm). Ekstrak daun H. perforata Merr.memiliki daya hambat yang lebih baik dibandingkan ekstrak akar.
STANDARISASI EKSTRAK ETIL ASETAT KAYU SANREGO (LUNASIA AMARA BLANCO) Anam, Syariful; Yusran, Muhammad; Trisakti, Alfred; Ibrahim, Nurlina; Khumaidi, Ahmad; Ramdanil, Ramdanil; Zubair, Muhammad Sulaiman
Natural Science: Journal of Science and Technology Vol 2, No 3 (2013): Volume 2 Number 3 (December 2013)
Publisher : Univ. Tadulako

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Lunasia amara Blanco is a popular medicinal plant which is known as aphrodisiac in South Sulawesi Province. Biological activity as antibacterial, anticancer dan antituberculosis were also scientifically reported. This study is to ascertain the safety and quality of the plant extract by standardization procedures mentioned in literature, including specific and non-specific parameters. The result showed that ethyl acetate wood extract of L. amara Blanco, which is brown viscous extract, astringent to the taste and characteristic odor, contain water-soluble extractive matters of 23,95 ± 2,192 %, ethanol-soluble extractive matters of 67,05 ± 3,61 %, water content of 5,33 ± 0,407 %,  total ash content of 0,65 ± 0,199 %, acid-insoluble ash content of 0,58 ±0,225 %, density of 0,7734 ± 0,0016 (5%) and 0,7957 ± 0,0021 (10%), total contaminant number of bacteria and fungus of each < 1 x 104 colony/g, and Pb concentration of 10,59 ± 0,239 mg/kg. Ethyl acetate wood extract of L. amara Blanco has been qualified as standardized extract. Therefore, this study can be a reference for identification and control quality of the extract as a herb-medicine material
FORMULASI KRIM ANTIOKSIDAN EKSTRAK ETANOL BAWANG HUTAN (ELEUTHERINE PALMIFOLIA L. MERR) Sharon, Nela; Anam, Syariful; Yuliet, Yuliet
Natural Science: Journal of Science and Technology Vol 2, No 3 (2013): Volume 2 Number 3 (December 2013)
Publisher : Univ. Tadulako

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Abstract

Eleutherine palmifolia L. Merr having antioxidant activity, its because of contain flavonoid, fenolat and tanin. These compounds have effect in  preventing of free radical which cause premature aging. Research on ethanol extract of E. palmifolia L. Merr which formulated into antioxidant cream have been done by applying variation of TEA emulgator and stearic acid. That variation  were F1 (2% : 6%), F2 (3% : 12%) and F3 (4% : 18%). Determination of antioxidant activity  was done based on DPPH methode, and the Value is 98,210%. Furthermore, evaluation on  physical characteristics was done based on organoleptic test, homogenity, pH, viscosity and stability of cream preparation. Observation was done during 21 days. Obtained data were then analyze by student-t test in comparing of the value between fresh and 21 days after storage. the pH of cream F1, F2 and F3 were not showed significant decrease, while DPPH reduce percent of F1, F2 and F3 were showed significant decrease.  In contrast, viscosity of cream F3 was significant decrease. Antioxydant activity extract based on DPPH reduce percent value of F1, F2, F3 at the first day were 96,822%, 97,123% and 96,896%, respectively. Whereas that value  at 21nd days after storage  were 89,036%, 89,726% and 90,564%, respectively.  Our result showed that E. palmifolia L. Merr ethanol extract  can be formulated as a cream preparations. F2 physical properties was  fullfill standart quality, which it was used TEA emulgator and stearic acid proportion of 3% : 12%.  
Co-Authors Abd. Rahman Razak Agus Ritna Agustinus Widodo Ahmad Khumaidi Akhmad Khumaidi Alfred Trisakti Amir Galeb Al Amrie Andi Muraqmi Apriyanto S. Tapundu Arba, Muhammad Arif Prasetyo, Arif Arisca, Windhy Nur Armini Syamsidi Asriana Sultan Astrid Natalia Alasa Ceria Arun Venagaya Cikal, Richie Annisa David Pakaya Dewi Permatasari DEWI RISMAYANTI Djaleha, Helena Fransisca Fadlia Fadlia Fahmi, Firdaus Yulian Febriyanto Rerung FIRMANITA DWIMURTI Fitrianingsih, Fera Hidayatullah Hidayatullah Ian Dianto Ibrahim Jantan Ihwam Ihwan Iklima Indriani, Meri Feki Irna Olvaliani Aimang Ivan Ivan Jamaluddin Jamaluddin Khusnul Diana Kurniawan, Filla Febri Leny Maryana Maliki, Ibnu Akbar Megawati Megawati Mohamed Ali Ghandourah Muhajir Lamasai Muhamad Rinaldhi Tandah Muhammad Akhsa Muhammad Sulaiman Zubair Muhammad Yusran Musyahidah Musyahidah Nela Sharon Ni Luh Yeni Safitri Nur Khairiyah Nurlina Ibrahim Nurul Ambianti Purnamasari Zainal, Amalia Ramadhanil Pitopang Ramadhanil pitopang Ramadhanil Ramadhanil Ramdanil Ramdanil Richma Hidayati Ritha Pratiwi Saipul Maulana Septiliana, Lilla Siti Hamdanah Siti Nuari Sri Normayunita, Sri Syaputra, Gunawan Tunreng, Muh. Fadil Usman Vini Mandasari Wa Ode Irma Indrayangingsih Walied Mohamed Alarif Yonelian Yuyun Yuliet Yuliet Yusriadi Yusriadi