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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Jurnal Ilmu Pangan dan Hasil Pertanian Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Canrea Journal: Food Technology, Nutritions, and Culinary Journal Jurnal Ilmu dan Teknologi Perikanan
apri dwi anggo
Departemen Teknologi Hasil Perikanan Fakultas Perikanan Dan Ilmu Kelautan Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Environmental Science Immunology & microbiology Public Health Other

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Pengaruh perbedaan waktu ekstraksi terhadap karakteristik kalsium Halimeda opuntia: The effect of different extraction time on the characteristics of calcium from Halimeda opuntia Daveza, Hisyam Pranata; Wijayanti, Ima; Anggo, Apri Dwi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 1 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i1.56126

Abstract

Halimeda opuntia adalah jenis alga hijau yang kaya akan kalsium karbonat. Metode ekstraksi kalsium dari H. opuntia terutama untuk durasi waktu ekstraksi masih belum optimal. Penelitian ini bertujuan untuk menentukan waktu ekstraksi terbaik kalsium dari H. opuntia melalui parameter kadar kalsium, warna, mikrostruktur, ukuran partikel, dan keberadaan gugus fungsi kimiawi. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) satu faktor, yaitu waktu ekstraksi 1, 2 dan 3 jam. Ekstraksi yang digunakan, yaitu sol gel dalam larutan HCl 1 N pada suhu 90°C. Parameter yang dianalisis meliputi rendemen, kadar kalsium, air, abu, protein, warna, ukuran partikel, mikrostruktur (SEM), dan gugus fungsi kimiawi (FTIR). Hasil penelitian menunjukkan bahwa waktu ekstraksi berpengaruh nyata terhadap rendemen, kadar kalsium, abu, lightness (L*), redness/greenness (a*), yellowness/blueness (b*), ΔE, dan whiteness index (WI). Perlakuan terpilih pada waktu ekstraksi 1 jam dengan kadar kalsium tertinggi, yaitu 30,79±0,56%. Rendemen, kadar air, abu dan protein perlakuan terbaik, yaitu 24,11±0,57%, 3,36±0,56%, 81,76±0,36%, dan 0,90±0,10%. Nilai L*, a*, b*, ΔE, dan WI berturut-turut adalah 83,83±0,65; 0,34±0,05; 8,84±0,21; 13,01±0,48; dan 84,29±0,65%. Waktu ekstraksi yang lebih lama meningkatkan rendemen kalsium namun terjadi degradasi serta warna yang makin menguning. Analisis mikrostruktur perlakuan ekstraksi 1 jam menunjukkan permukaan menyerupai bola yang terlihat kasar dan tidak menggumpal dengan ukuran partikel seragam 3,25 nm. Hasil analisis gugus fungsi menunjukkan keberadaan kalsium karbonat dalam spektra FTIR H. opuntia teridentifikasi pada gelombang 1.407,1 cm-1 dan 872,2 cm-1. Waktu ekstraksi 1 jam dinilai optimal untuk mengekstrak kalsium dari H. opuntia.
Purification of phycocyanin from Spirulina platensis by combination diafiltration/ ultrafiltration membrane Agustini, Tri Winarni; Anggo, Apri Dwi; Yuliani, Yuliani
jurnal1 VOLUME 8 ISSUE 2, DECEMBER 2025
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v8i2.1121

Abstract

Phycocyanin, a blue pigment derived from the cyanobacterium Spirulina platensis, is typically purified through either single-step or multi-step processes. The purity of phycocyanin is crucial for its applications as a food colorant, in cosmetics, as a biomarker, and for analytical purposes. In this study, we report the purification of phycocyanin using a multi-step process involving extraction, ammonium sulfate precipitation, and diafiltration/ultrafiltration (DF/UF) with cycle times of 20, 30, and 40 minutes. The aim of this research to determine the effect of DF/UF time on flux, retention rates and purity on phycocyanin purification. The combination of DF/UF allowed the improving the purity of the phycocyanin in the retentate fraction. Sample that have been extracted and ammonium sulphate precipitation, were purified by ultrafiltration membrane using of hydrophilic polyethersulfone (PESH) membrane of 50 kDa MWCO. The DF/UF system was operated at 1.0 ± 0.1 Psi and at 24 ± 1oC. sample were collected during both DF/UF process in order to evaluated flux, retention rates and purity ratio of phycocyanin. The best DF/UF process were operate at 40 min per cycle, with a mean permeate flux 6.89 L/m2h and retention rate 96.99% was found to be optimal. This process resulted in phycocyanin with purity and concentration of 2.54 and 0.30 mg/mL, respectively. Hence, the DF/UF process were operated at 40 min per cycle potential for a biomarker application.
Co-Authors - Sumardianto A Suhaeli Fahmi Abdul Syakur Abiza Winner Natanael Adilla Wahyu Nugraheni Aditya Cahya Putra Aditya Nugroho Afwan Widhiharto Agung Purnama Agus Hariyanto Agus Tri Setyo Wicaksono Ali Djamhuri Andi Ayu Irawati, Andi Ayu Andiani Ulfa Saputri Andini, Lucky Ardila Prasinta Devi Ardya Risma Firmansyah Arnyta Restu Pratiwi, Arnyta Restu Aryna Conny Tamaya Cahya, Ira Cindy Dwi Daveza, Hisyam Pranata Dayu Bestari Diah Nur Aisyah Tri Meiyani Diana Nur Afifah, Diana Nur Egidya Safitri Eko Nurcahya Dewi Eko Nurcahya Dewi Elfrido Christian Dewantara Faidha Santika, Faidha Faishal, Imam Farraz Fajar, Rahmadian Fara Sabilla Ulfa Farhan Alfarobi Karim Farisan Hilmana Ramadhan Fathin Alyani, Fathin Ferdianto, Richki Ferry Istanto Firman Multazam Fronthea Swastawati Harys Desvian Wibisono Hasthi, Saputra Helmy Mustafida Hilmi, Muhammad Zuhal Ibnu Bachtiar Ida Harjuno Ima Wijayanti Imam Restu Wibowo Jannah, Nabila Tsarwatul Kartika Anjar Sari Koko Seviyanto Laras Rianingsih Liberta Ibi Patria Lilin Budiati Lukita Purnamayati Ma'ruf, Widodo Farid Medi Prasetyo Meilinda Hardianti, Meilinda Mimin Sugiarti Namaskara, Fadhillah Sanitya Nurafifah, Diyah Ayu Nurina Almas Shabrina Pramitha Nurma Deviyanti, Pramitha Nurma Purnamayati, Lukita Putut Har Riyadi Rahmaningsih, Astuti Rani Tri Mustika Novitasari Ratna Ibrahim Ratna Ibrahim Ratna Ibrahim Retno Ayu Kurniasih Rian Adiaprana, Rian Riana Rachmawati Rianingsih, Laras '- Romadhon Romadhon Sindi Clarista Efendi Slamet Suharto, Slamet Subianto, Purwanti Fadiah Kusuma Sari Sumardianto - Tambunan, Van Rhein Titi Surti Tri Wahyuningsih Tri Winarni Agustini Tri Wisnu Susilo Umi Nur Safitri Wahyu Nur Widiyanto Widodo Farid Ma'ruf Widodo Farid Ma’ruf Wijayanti, Ima '- Wijayanti, Ph.D, Ima Y S Darmanto y S Darmanto Yhuda Ardiansyah, Yhuda YS Darmanto Yudhomenggolo Sastro Darmanto Yudhomenggolo Sastro Darmanto Yuliani Yuliani