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Molecular Docking Study of Bioactive Compounds from Curcuma aeruginosa Roxb. as Antioxidant and Anticancer Activities Ambarsari, Laksmi; Nurjanah, Siti; Artika, I Made; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.327

Cancer is one type of dangerous disease that is increasing every year. Free radicals are the cause of gene mutations (cancer). Cancer will develop uncontrollably due to the occurrence of the cell cycle and the presence of compounds that trigger cell proliferation and inhibit the process of apoptosis. This cancer treatment is carried out by giving cyclin-dependent kinase2 (CDK2) and cyclooxygenase-2 (COX-2) inhibitor drugs to inhibit cancer development, as well as lipoxygenase (LOX) inhibitor drugs for the formation of free radicals. Curcuma aeruginosa RoxB., the plant, is known to have the potential for antioxidant and anticancer properties. This study aims to determine the molecular interaction between the dominant compound in the ethanol extract of C. aeruginosa with CDK2, COX-2, and LOX receptors. The type of ligand interaction with the receptor was determined through the parameters of affinity energy (∆G), inhibition constant (Ki), type of interaction, and percentage of binding site similarity (%BSS). The results showed that the gajutsulacton A had the best potential in inhibiting CDK2. The cucumenol may be a COX-2 inhibitor, and there are no compounds that can inhibit LOX as well as an antioxidant. Thus, our findings demonstrate the potential for C. aeruginosa bioactive to serve as anticancer candidate molecules against CDK2 and COX-2 receptors.

Potential of Sunda Porcupine (Hystrix javanica) Quills Extract as a Wound Healing Medicine: A Review of the Bioactive Components and Mechanisms Yulianto; Nurcholis, Waras; Artika, I Made
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.329

Sunda porcupine (Hystrix javanica), a native species of Indonesia, is renowned for its distinctive sharp quills enveloping its body. Despite its reputation as a spiky creature, the use of porcupine quills in traditional medicine in Indonesian society, especially for toothache and stomach ulcers, shows that there are benefits as an alternative to herbal medicine that has yet to be fully explored. Even outside Indonesia, North American porcupine quills are reported to have antibiotic properties related to the free fatty acids that coat the spines. Extracts of Sunda porcupine quills have also shown interesting antibacterial activity. Several bioactive compounds have been found in Sunda porcupine quills extract, including alkaloids, flavonoids, saponins, steroids, triterpenoids, and peptides. These compounds have various health benefits, such as analgesic, anti-inflammatory, and antimicrobial. In addition, the antibacterial activity test on the extract showed strong potential against the growth of gram-positive bacteria. The activities of secondary metabolites that play a role in helping wound healing are anti-inflammatory, antioxidant, and antibacterial/antimicrobial activities. Wound healing mechanisms involve several complex interactions of various cellular and molecular processes. Recent research has revealed the great potential of extracts from Sunda porcupine quills as a wound healing medicine. Although the potential of Sunda porcupine quills extract as a wound healing medicine has attracted attention, this research still has limitations. Critical measures, such as in vivo and in vitro testing, still need to be improved, leaving gaps in information that require further research. The challenge of an in-depth understanding of the activity and mechanisms of bioactive compounds is the focus on optimizing the utilization of Indonesia's natural potential. This article reviews the bioactive components of Sunda porcupine quills extract and their mechanism as a wound healing agent.

Enhancement of colour quality, growth, and health status of rainbow Kurumoi fish Melanotaenia parva through dietary synthetic carotenoids supplementation Meilisza, Nina; Suprayudi, Muhammad Agus; Jusadi, Dedi; Junior, Muhammad Zairin; Artika, I Made; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Carotenoids were known as pigment sources, the precursor of vitamin A, potential antioxidant and can improve the health status of fish. Furthermore, there are also studies that reveal the role of carotenoids in bone formation and metabolism. This study was conducted to determine the effect of different carotenoids at two different doses in the feed on growth, pigmentation, vitamin A conversion, blood profile, antioxidant activity, and calcium bone of the rainbow fish. Twenty-one aquariums with a volume of 20 L each stocked with 20 fish (1.08 ± 0.03 g of body weight and 4.56 ± 0.07 cm of body length). The experiment applied seven experimental diets (in triplicates) consisted of three types of carotenoids were astaxanthin (AS), canthaxanthin (CS), and lutein (LS) and two doses (130 and 260 mg/kg), i.e. AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 and basal (without carotenoids) as the control. The fish were fed for 56 days of experimental period. The results showed that carotenoid diets were able to increase growth, total carotenoids, percentages of chromatophores, vitamin A conversion, erythrocyte, leukocytes, packed cell volume (PCV), neutrophils, and hemoglobin (Hb) compared to the control. Fish fed dietary astaxanthin at a level of 260 mg/kg was superior compared to other diets. Dietary carotenoids were also capable of decreasing the endogenous antioxidant activity of superoxide dismutase (SOD) and malonyl dialdehyde (MDA) and increased the calcium level in fish bone than basal diet. Keywords: carotenoids, growth, health status, Melanotaenia parva, pigmentation ABSTRAK Karotenoid diketahui sebagai sumber pigmen, prekursor vitamin A, antioksidan potensial dan dapat meningkatkan status kesehatan ikan. Selain itu, karotenoid juga memiliki peran dalam formasi dan metabolism tulang. Penelitian ini dilakukan untuk mengevaluasi pengaruh jenis dan dosis karotenoid yang berbeda terhadap pertumbuhan, pigmentasi, konversi vitamin A, gambaran darah, aktifitas antioksidan dan kalsium tulang ikan rainbow Kurumoi. Sebanyak 20 ekor ikan (bobot tubuh rata-rata 1,08 ± 0,03 g dan panjang total rata-rata 4,56 ± 0,07 cm) dan diberi makan pakan yang mengandung karotenoid. Pakan uji terdiri atas tiga jenis karotenoid dengan tiga ulangan yaitu astaksantin (AS), cantaksantin (CS), dan lutein (LS) dan dua dosis (130 dan 260 mg/kg) dikodekan dengan AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 dan basal (tanpa karotenoid) sebagai kontrol. Ikan diberi makan selama 56 hari pemeliharaan. Hasil penelitian menunjukkan bahwa karotenoid dapat meningkatkan pertumbuhan, total karotenoid, persentase kromatofora, konversi vitamin A, eritrosit, leukosit, hematokrit, neutrofil, dan hemoglobin dibandingkan dengan kontrol. Ikan yang diberi pakan mengandung astaksantin 200 mg/kg lebih baik dibandingkan denga pakan uji lainnya. Pakan yang mengandung karotenoid juga mampu menurunkan antioksidan endogenus superoxide dismutase (SOD) dan malonyl dialdehyde (MDA), serta meningkatkan kalsium tulang ikan dibandingkan pakan kontrol. Keywords: Melanotaenia parva, karotenoid, pertumbuhan, pigmentasi, status kesehatan.

Pemurnian alfa-selulosa dari baglog bekas jamur tiram putih (Pleurotus ostreatus) menggunakan NaOH dan hidrolisis sulfat (Purification of alpha-cellulose from ex-baglog of white oyster mushroom (Pleurotus ostreatus) using NaOH and sulfate hydrolysis) Hyakansa HANIF; TRI - PANJI; Firda DIMAWARNITA; I Made ARTIKA
Menara Perkebunan Vol. 87 No. 1 (2019): 87 (1), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i1.325

Oil Palm Empty Fruit Bunches (OPEFB) are the largest cellulose waste produced by oil palm plantations. Each processing of one ton of Fresh Fruit Bunch (FFB) will produce OPEFB as much as 22-23% or 220-230 kg. OPEFB waste has not been utilized optimally by most palm oil mills and communities. It was reported that OPEFB contains 32.57% cellulose, 27.70% hemicellulose, and 26.49% lignin. The purity of cellulose component in OPEFB can be increased by delignification and hydrolysis of OPEFB. This research aims to produce alpha-cellulose from OPEFB with the highest purity level. The stages of the process include biologically delignification using white oyster mushroom (Pleurotus ostreatus), chemical delignification using 17,5% NaOH solution, and combination of treatment using solution of 17,5% NaOH and hydrolysis using 10% H2SO4solution. The analysis was carried out using the Fourier Transform Infrared Spectroscopy (FTIR). The best result of alpha-cellulose purity was by the treatment of delignified NaOH baglog resulting in 97.43% alpha-cellulose content, 4.47% pentosan hemicellulose level, and undetectable lignin levels.The results of FT-IR spectrum from alpha-cellulose obtained from OPEFB has shown functional groups contained in alpha-cellulose polymers including O-H groups at wave number 3289 cm-1, C-H groups at wave number 2901 cm-1, and functional groups C-O at wave number 1372 cm-1.[Key words: hydrolysis, FT-IR, mushroom, bleaching, waste] Abstrak Tandan kosong kelapa sawit (TKKS) adalah limbah terbesar yang dihasilkan oleh perkebunan kelapa sawit. Setiap pengolahan satu ton tandan buah segar (TBS) akan dihasilkan TKKS sebanyak 22-23% atau 220-230 kg. Limbah TKKS belum dimanfaatkan secara optimaloleh sebagian besar pabrik kelapa sawit (PKS) dan masyarakat. Dari hasil analisis kimiakomposisi selulosa, hemiselulosa, dan lignin pada TKKSsecara berturut-turut ialah ; 32,57%, 27,70%, 26,49%. Kandungan komponen selulosa dalam TKKSdapat ditingkatkan kemurniannya dengan cara delignifikasi dan hidrolisis. Penelitian ini bertujuan pemurnian alfa–selulosa dari TKKS dengan tingkat kemurnian tertinggi.Tahapan pembuatan alfa-selulosameliputi delignifikasi secara biologis menggunakan jamur tiramputih(Pleurotus ostreatus), delignifikasi secara kimia menggunakan larutan NaOH 17,5%, dan kombinasi perlakuan menggunakan NaOH 17,5% dan larutanH2SO410%. Analisis dilakukan menggunakanFourier Transform Infrared Spektroscopy (FT-IR). Hasil pemurnian alfa-selulosa terbaik ialah melalui sisa baglog terdelignifikasi NaOH menghasilkan kadar alfa-selulosa 97.43%, kadar pentosan hemiselulosa 4.47%, serta kadar lignin tidak terdeteksi. Hasil spektrumFT-IR darialfa–selulosayang diperoleh dari TKKStelahmenunjukkan gugus-gugus fungsi yangterdapat di dalam polimeralfa–selulosadiantaranyagugus O-H pada bilangan gelombang 3289 cm-1, gugus C-H pada bilangangelombang2901cm-1, dan gugus fungsi C-O pada bilangan gelombang 1372 cm-1. [Kata kunci: hidrolisis, FT-IR, jamur, pemutihan, limbah]

Molecular docking: Bioactive compounds of Mimosa pudica as an inhibitor of Candida albicans Sap 3 Gholam, Gusnia Meilin; Firdausy, Iman Akhyar; Artika, I Made; Abdillah, Ramadhani Malik; Firmansyah, Ridwan Putra; Irsal, Riyan Alifbi Putera; Zahra, Hafizh
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.4

Candida albicans (C. albicans) is a commensal microbiota that resides in humans. However, in certain cases, C. albicans can infect and cause several diseases to humans. This study aimed to investigate the interaction between Mimosa pudica bioactive compounds and C. albicans Sap 3. Molecular docking analysis was carried out using YASARA structure. The procedures involved preparation of ligands and target receptor, molecular docking, data analysis and visualization. All 3D ligands were downloaded from PubChem NCBI, while target receptor was downloaded from RCSB PDB. The interaction between Mimosa pudica bioactive compounds against Sap 3 resulted in a binding energies ranges from 5,168 – 7,480 kcal/mol and most of the interactions formed were relatively strong. Furthermore, the test ligands had contact with the catalytic residues and substrate binding site pockets S1/S2/S3/S4 on the target receptor. Bioactive compounds of Mimosa pudica have relatively good interactions in inhibiting C. albicans Sap 3

Minyak atsiri Kapulaga (Elettaria cardamomum) sebagai inhibitor Sap 5 Candida albicans penyebab kandidiasis vulvovaginalis (KVV) secara in silico Gusnia Meilin Gholam; Kurniasih, Rini; I Made Artika
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.5

ABSTRACT Vulvovaginal candidiasis (VVC) is a disease caused by the inflammatory process of the vulva and vaginal mucosa caused by Candida sp., mainly Candida albicans. This study aimed to analyze the molecular interaction between the volatile oil in cardamom and Sap 5 as an inhibitor of Candida albicans causing VVC through In silico molecular interaction analysis. The methods used are analysis of homology, structural quality, and essential areas, receptor and ligand preparation, gridbox validation, virtual screening, Lipinski prediction and toxicity, and ligand-receptor interaction visualization analysis. The results showed that essential oils have the potential to inhibit Sap 5 through molecular bonding and produce interactions in the form of hydrogen bonds, electrostatic bonds, and hydrophobic interactions. The best test ligands were Geranyl acetate (-6.78 kcal/mol), Alpha-terpinyl acetate (-6.07 kcal/mol), 1,8-Sineol (-5.47 kcal/mol), and Linalool (-5.06 kcal/mol). The test ligands have contact with catalytic residues on Asp32/Asp218. In addition, the properties of these ligands also meet the Lipinski and Toxicity rules, so they can be predicted to be safe. Keywords: Candida albicans, Cardamom, Essential oil, In silico, Sap 5 ABSTRAK Kandidiasis vulvovaginalis (KVV) merupakan penyakit akibat dari proses inflamasi vulva dan mukosa vagina yang disebabkan oleh Candida sp. utamanya Candida albicans. Penelitian ini bertujuan untuk menganalisis interaksi molekuler antara minyak atsiri yang terkandung pada kapulaga dengan Sap 5 sebagai inhibitor Candida albicans penyebab KVV melalui analisis interaksi molekuler secara In silico. Metode yang digunakan yaitu analisis homologi, kualitas struktur, dan daerah penting, preparasi reseptor dan ligan, validasi gridbox, penapisan virtual, prediksi Lipinski dan toksisitas, dan analisis visualisasi interaksi ligan-reseptor. Hasil penelitian menunjukkan bahwa minyak atsiri mempunyai potensi menghambat Sap 5 melalui penambatan molekuler dan menghasilkan interaksi berupa ikatan hidrogen, ikatan elektrostatik, dan interaksi hidrofobik. Golongan ligan uji yang terbaik yaitu Geranil asetat (-6.78 kkal/mol), Alfa-terpinil asetat (-6.07 kkal/mol), 1,8-Sineol (-5.47 kkal/mol), dan Linalool (-5.06 kkal/mol). Ligan uji tersebut mempunyai kontak residu katalitik pada Asp32/Asp218. Selain itu, sifat ligan tersebut juga memenuhi aturan Lipinski dan Toksisitas, sehingga dapat diprediksi aman. Kata kunci: Candida albicans, Kapulaga, Minyak atsiri, In silico, Sap 5

Evaluasi Faktor Yang Mempengaruhi Ekstraksi Rimpang Temu Ireng Berdasarkan Aktivitas Penghambatan α-Glukosidase Artika, I Made; Ambarsari, Laksmi; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 3 No. 2 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i2.57

Terdapat beberapa faktor yang dapat digunakan dalam ekstraksi rimpang temu ireng yang memiliki aktivitas farmakologi. Penelitian ini bertujuan untuk mengidentifikasi faktor pH, konsentrasi etanol, suhu, waktu, dan rasio cairan-padatan dalam proses ekstraksi rimpang yang memberikan kontribusi signifikan sebagai inhibitor enzim a-glukosidase. Rancangan optimasi dilakukan dengan menggunakan faktoral fraksional 2(5-1). Urutan hasil dari ekstrak yang paling berkontribusi sebagai inhibitor enzim a-glukosidase: konsentrasi etanol > rasio cairan terhadap padatan > suhu > waktu > pH. Diantara faktor yang dianalisis menunjukkan bahwa konsentrasi etanol dan interaksi antara pH dan konsentrasi etanol merupakan faktor yang signifikan sebagai inhibitor enzim a-glukosidase. Dengan demikian, rimpang temu ireng memiliki potensi sebagai anti-hiperglikemia alami.

Molecular Docking Study of Bioactive Compounds from Curcuma aeruginosa Roxb. as Antioxidant and Anticancer Activities Ambarsari, Laksmi; Nurjanah, Siti; Artika, I Made; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.327

Cancer is one type of dangerous disease that is increasing every year. Free radicals are the cause of gene mutations (cancer). Cancer will develop uncontrollably due to the occurrence of the cell cycle and the presence of compounds that trigger cell proliferation and inhibit the process of apoptosis. This cancer treatment is carried out by giving cyclin-dependent kinase2 (CDK2) and cyclooxygenase-2 (COX-2) inhibitor drugs to inhibit cancer development, as well as lipoxygenase (LOX) inhibitor drugs for the formation of free radicals. Curcuma aeruginosa RoxB., the plant, is known to have the potential for antioxidant and anticancer properties. This study aims to determine the molecular interaction between the dominant compound in the ethanol extract of C. aeruginosa with CDK2, COX-2, and LOX receptors. The type of ligand interaction with the receptor was determined through the parameters of affinity energy (∆G), inhibition constant (Ki), type of interaction, and percentage of binding site similarity (%BSS). The results showed that the gajutsulacton A had the best potential in inhibiting CDK2. The cucumenol may be a COX-2 inhibitor, and there are no compounds that can inhibit LOX as well as an antioxidant. Thus, our findings demonstrate the potential for C. aeruginosa bioactive to serve as anticancer candidate molecules against CDK2 and COX-2 receptors.

Potential of Sunda Porcupine (Hystrix javanica) Quills Extract as a Wound Healing Medicine: A Review of the Bioactive Components and Mechanisms Yulianto; Nurcholis, Waras; Artika, I Made
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.329

Sunda porcupine (Hystrix javanica), a native species of Indonesia, is renowned for its distinctive sharp quills enveloping its body. Despite its reputation as a spiky creature, the use of porcupine quills in traditional medicine in Indonesian society, especially for toothache and stomach ulcers, shows that there are benefits as an alternative to herbal medicine that has yet to be fully explored. Even outside Indonesia, North American porcupine quills are reported to have antibiotic properties related to the free fatty acids that coat the spines. Extracts of Sunda porcupine quills have also shown interesting antibacterial activity. Several bioactive compounds have been found in Sunda porcupine quills extract, including alkaloids, flavonoids, saponins, steroids, triterpenoids, and peptides. These compounds have various health benefits, such as analgesic, anti-inflammatory, and antimicrobial. In addition, the antibacterial activity test on the extract showed strong potential against the growth of gram-positive bacteria. The activities of secondary metabolites that play a role in helping wound healing are anti-inflammatory, antioxidant, and antibacterial/antimicrobial activities. Wound healing mechanisms involve several complex interactions of various cellular and molecular processes. Recent research has revealed the great potential of extracts from Sunda porcupine quills as a wound healing medicine. Although the potential of Sunda porcupine quills extract as a wound healing medicine has attracted attention, this research still has limitations. Critical measures, such as in vivo and in vitro testing, still need to be improved, leaving gaps in information that require further research. The challenge of an in-depth understanding of the activity and mechanisms of bioactive compounds is the focus on optimizing the utilization of Indonesia's natural potential. This article reviews the bioactive components of Sunda porcupine quills extract and their mechanism as a wound healing agent.

Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ∆ 420/minute/mg protein). Key words: in vitro calli, shoots, crude protein, chitinase and peroxidase activities

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