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Journal : Journal of Tropical Crop Science

The Study of Organic Fertilizers Application on Two Soybean Varieties in Organic Saturated Soil Culture Aziz, Sandra Arifin; Melati, Maya; Ramadhani, Elrisa
Journal of Tropical Crop Science Vol 3 No 1 (2016): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.895 KB) | DOI: 10.29244/jtcs.3.1.19-27

Abstract

Indonesia is facing soybean shortage and the low productivity of soybean production from decreasing acreage. Saturated soil culture (SSC) could be one of the solutions for marginal land with drainage problem. Saturated soil culture is cultivation technology that gives continuous irrigation and maintains water depth constantly and makes soil layer in saturated condition. Farmers can use on-farm inputs that are normally available at the production site. An organic farming system may be able to ensure local and regional food security through continuous production. The study was conducted at Cikarawang Experimental Station of Bogor Agricultural University, Indonesia, from December 2009 to February 2011. The objective of the research was to determine the influence of organic fertilizer application on the productivity of two varieties of soybean, “Anjasmoro” and “Wilis”,    in organic saturated soil culture conducted in two cropping seasons. Prior to the experiment all plots were applied with 2 ton.ha-1 of dolomite, 2 ton.ha-1 of rice hull ash, and 10 ton.ha-1 of chicken manure. The experiment of the first season used split plot design with six replications. The main-plot was organic fertilizer that consisted of chicken manure only (20 ton.ha-1), chicken manure (10 t.ha-1) + Centrosema pubescens (4.2 t.ha-1), chicken manure (10 ton.ha-1) + Tithonia diversifolia (4.2 t.ha-1). The sub-plot was soybean varieties “Anjasmoro” and “Wilis”. The soybean productivity was not affected by the application of organic fertilizers. “Willis” productivity (1.98 t.ha-1) was higher than “Anjasmoro” (1.80 t.ha-1). The experiment of second season used split-split plot design with three replications. The main-plot consisted of 50 and 100% fertilizer rate of the first cropping season; the sub-plot was the same types of organic fertilizer with soybean varieties as sub-sub-plot. Rate of fertilizer, types of organic fertilizer and soybean variety did not affect productivity. Application of 50 and 100% rate of fertilizer in the second season produced 2.41 and 2.55 t.ha-1 of dry seeds, respectively. Soybean plants treated with chicken manure, green manure from C. pubescens and T. diversifolia produced 2.45, 2.50 and 2.49 t.ha-1 of dry seeds, respectively. “Anjasmoro” and “Wilis” produced 2.50 and 2.45 t.ha-1 of dry seeds, respectively. Productivity in the second season was 26.26-36.61% higher than those of the first cropping season.
Increasing Growth Rate and Production of Bioactive Compounds Curcuminoid and Xanthorrhizol in Javanese Turmeric (Curcuma xanthorrhiza Roxb.) Rhizomes with Biso Zyme Application Aziz, Sandra Arifin; Ridwan, Taopik; Batubara, Irmanida
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.959 KB) | DOI: 10.29244/jtcs.5.3.103-110

Abstract

The bioactive compounds from Javanese turmeric (Curcuma xanthorrhiza Roxb.), curcuminoid and xanthorrhizol, have been used to treat human diseases. This research aims to study the Javanese turmeric growth, its curcuminoid and xanthorrhizol content using Biso Zyme with different concentration and interval spraying. Biso Zyme is is conjugated enzyme concentrate (CEC) produced by yeast extracts that can be applied to the plant to increase and improve plant yields. The treatments were arranged in a randomized block factorial design with three replications. The concentrations of Biso Zyme were 0.0, 0.1, and 0.05 %, with monthly spraying intervals at 1, 2, 3, 4 and 5 months after planting (MAP). The results showed that the highest leaf area index was 2.71 cm2g-1  followed by leaf area ratio which was 66.47 cm2.g-1 with 0.1% Biso Zyme application. Relative growth rate was 0.048 g.day-1while net assimilation rate was 0.00092 g.cm-2.day-1 without Biso Zyme application. The highest fresh and dry weight was those treated with 0.05% Biso Zyme applied at 4 to 5 MAP, i.e., 444.44 g and 68.72 g, respectively. The highest curcuminoid and xanthorrhizol contents were 0.435% and 1.505%, respectively, with 0.05% Biso Zyme spraying interval at 4 to 5 MAP, and 0.1%  Biso Zyme with sprayed at 1 to 5 MAP. Thin layer chromatography (TLC) analysis detected standard curcuminoid at Rf 0.23 and xanthorrhizol at Rf 0.66 with dichloromethane:chloroform eluents. Keywords: curcuminoid, Javanese turmeric, Leaf Area Index, organic fertilizer, xanthorrhizol 
Diversity of Functional Soil Microbes in Manures and Its Effect on Organic Mustard Green (Brassica juncea) Production Kurniawati, Ari; Melati, Maya; Aziz, Sandra Arifin; Purwono, Purwono
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.873 KB) | DOI: 10.29244/jtcs.6.01.60-66

Abstract

The application of organic fertilizer, especially manures, for crop production has long-term effects for soil nutrients availability and improvement of soil structure. The improvement of soil properties involves interactions of various types of microorganism in the soil. The research aimed to study the effects of different types of manures on the diversity of functional soil microbes and its effects on organic green mustard production. The experiment was conducted at IPB organic research field, Cikarawang, Darmaga, Bogor, from April to June 2015. The experiment used a randomized complete block design with two factors; the first factor was types of manure, i.e. chicken, cow, and goat manures; the second factor was manure rates, i.e. 0 and 10 ton.ha-1, so there were six treatments in total, replicated three times. The results showed that chicken manure application resulted in a higher mustard green yield and soil-P content than application of goat manure. The addition 10 ton.ha-1 of manures increased C-organic, N, K, and C/N ratio in soil significantly, but mustard green production was not affected. The diversity of microbe population of the soil treated with all types of manures was high. The addition 10 ton.ha-1 of manures decreased the total number of microbes, but increased the number of cellulose-degrading microbes. The population of cellulose-degrading and phosphate-solubilizing microbes in the soil applied with cow manure was higher than those applied with the other manures.
Leaf Production Responses of Lampeni (Ardisia humilis Vahl.) to Types and Rates of Manure Application Aziz, Sandra Arifin; Wardani, Eska Ayu
Journal of Tropical Crop Science Vol 6 No 02 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.185 KB) | DOI: 10.29244/jtcs.6.02.98-104

Abstract

Ardisia humilis (Myrsinaceae), known as Lampeni (in Indonesia), is part of the wild flora in Southeast Asia and is known as a weed in some countries. The leaf has medicinal properties for some diseases. The leaves and fruit of this plant are believed to be a treatment for tuberculosis, lung disease, hepatitis, chronic bronchitis, and menstrual irregularities in women. Research into the cultivation of Lampeni is needed to support its usage in Indonesia. The objectives of the research reported here were to find the types and rates of manure to promote leaf production. An experiment was carried out at IPB Experimental Station, Cikarawang Dramaga, Bogor, Indonesia, from December to June 2016. The experiment was laid out in a split plot design with manure types (chicken and cow manure) as main plots and application rates (0, 5, 10, and 15 t ha-1) as sub-plots. The results of the experiment showed that vegetative growth increased with manure application. Total branch number, LAI, leaf fresh and dry weight were higher with 15 t.ha-1 chicken manure or 5 t.ha-1 cow manure application. Leaf analysis showed that pigment concentration responded negatively to increasing manure application and the highest pigment concentration was found on the plants without manure application.
Correlation of Leaf NPK and Leaf Pigments of Coleus atropurpureus L. Benth during Vegetative and Generative Phases Respita, Intan Annisa; Aziz, Sandra Arifin; Kurniawati, Ani
Journal of Tropical Crop Science Vol 6 No 03 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.003 KB) | DOI: 10.29244/jtcs.6.03.174-181

Abstract

Coleus atropurpureus L. Benth is a annual plant that has a distinctive leaf aroma and bitter taste. C. atropurpureus leaves contain phenolic compounds and antioxidants that can capture free radicals; free radicals play an important role in preventing various human diseases. A study was conducted to determine the correlation between leaf position (1st to 4th) at the vegetative and generative phases with leaf pigments, N, P, K, and total flavonoid concentrations. The results showed that leaf chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, anthocyanin, nitrogen, and total flavonoids were higher in the vegetative phase. Therefore, C. atropurpureus is better harvested in the vegetative phase, and the 2nd leaf position can be used as indicator for N, K, pigments and total flavonoid content.
Correlations of Nitrogen, Phosphorus, Potassium, Pigments and Total Flavonoids of Moringa oleifera Lam. Leaves in the Vegetative and Generative Phases Rasmani, Rasmani; Aziz, Sandra Arifin; Suketi, Ketty
Journal of Tropical Crop Science Vol 7 No 02 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.379 KB) | DOI: 10.29244/jtcs.7.02.75-85

Abstract

Moringa oleifera is universally known as the miracle plant or the tree of life. Moringa leaf extract contains phenolic acids and flavonoids, especially flavonols (quercetin, kaempferol, isorhamnetin glycosides) and flavones (apigenin). This study aimed to elucidate any correlations between nutrient, pigment, and flavonoid concentrations for different leaf positions and different growth phases. The results showed that pigment and total flavonoid concentrations increased from the 1st leaf to the 5th leaf, while nutrient concentration decreased. Pigment concentration, total flavonoid, and nutrient concentration were higher in the vegetative phase than the generative phase. The concentration of nutrients in the generative plants displayed a significant positive correlation with chlorophyll concentration. The 1st to 5th leaf of the vegetative plants can be used as indicator leaves for tissue analysis of the moringa plant.
Correlations between Leaf Nutrient Content and the Production of Metabolites in Orange Jessamine (Murraya paniculata L. Jack) Fertilized with Chicken Manure Karimuna, Siti Rahmah; Aziz, Sandra Arifin; Melati, Maya
Journal of Tropical Crop Science Vol 2 No 1 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.525 KB) | DOI: 10.29244/jtcs.2.1.16-25

Abstract

Plant secondary metabolites are unique sources for pharmaceuticals and food additives. Orange jessamine (Murraya paniculata) contains secondary metabolite that is beneficial to human health including lowering blood cholesterol levels, anti-obesity, and has the antioxidant capacity. Leaves of orange jessamine have several chemical constituents including L-cadinene, methyl-anthranilate, bisabolene, β-caryophyllene, geraniol, Carene, 5-guaiazulene, osthole, paniculatin, tannins, eugenol, citronelled, coumurrayin and coumarin derivatives. This study aimed to determine the correlation between leaf concentrations of N, P and K with leaf bioactive compounds following chicken manure application to the plants. The experiment was conducted at IPB Experimental Station at Cikarawang, Bogor (6o30' - 6o45' S, 106o30'-106o45' E) from March to November 2014 (250 m above sea level). The study used a randomized block design (RBD) with rates of chicken manure as a treatment, i.e. 0, 2.5, 5 and 7.5 kg per plant. Chicken manure was applied at 30 months after planting (MAP). Leaves were harvested by pruning the plants to a height of 75 cm above the soil surface. The results showed that the optimum rates of chicken manure to produce maximum fresh and dry leaf weight were 3.1 kg and 6.5 kg per plant, respectively. This rate was sufficient for leaf production at the first harvest (34 MAP) but was insufficient for the second harvest (38 MAP). K concentration of the leaves from different positions within the plant and leaf age positively correlated with leaf dry weight (P <0.01), but negatively correlated with total flavonoid content (P <0.01). Leaf P concentration was negatively correlated with dry weight of the 5th young leaves, or mature leaves from all positions. Leaf K concentration was categorized very high (3.59-4.10%), whereas leaf P concentration was high (0.28-0.29%) to very high (0.33-0.35%). The 5th mature leaves determined plant K requirements.Keywords: antioxidant, bioactive compounds, leaf position, organic, NPK leaf concentrations
Correlations between Leaf Nitrogen, Phosphorus and Potassium and Leaf Chlorophyll, Anthocyanins and Carotenoids Content at Vegetative and Generative Stage of Bitter Leaf (Vernonia amygealina Del.) Betty Tjhia; Sandra Arifin Aziz; Ketty Suketi
Journal of Tropical Crop Science Vol. 5 No. 1 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.936 KB) | DOI: 10.29244/jtcs.5.1.25-33

Abstract

Vernonia amygdalina Del. (Asteraceae) is a nutritional and medicinal plant that is used widely throughout tropical Africa and other countries. The leaves are proved to have strong antioxidant property. The concentration of chlorophyll a, chlorophyll b, anthocyanins and carotenoids in five different leaf positions of vegetative and generative phases were studied. The aim of the study is to understand and to correlate the content of the three nutrients and the bioactive compounds along the five leaf positions at vegetative and generative stage of the crop growth. The study showed that the concentration of chlorophyll a and b was higher, and anthocyanin concentration was almost zero in the generative than in the vegetative stage. The leaf concentration of N, P, and K were higher in vegetative than in generative stage. At vegetative stage, N positively correlated with chlorophyll a at the 1st leaf; K had strong correlations with anthocyanins at the 2nd and 3rd leaf and with chlorophyll a at the 2nd and 5th, and with chlorophyll b and carotenoids at the 5th leaf. At the generative stage, P had a strong positive correlation with carotenoids at 4th and 5th leaves, and K positively correlated with chlorophyll a and b at 5th leaf. The findings suggest that bitter leaf is better to be harvested at vegetative stage, and that potassium level is important to increase ­anthocyanins and carotenoids content.
Modification of The Spectrophotometric Analysis Protocol by Sims D. and Gamon to Analyze Leaf Pigment Content using Green Spinach (Amaranthus hybridus) as a Model Plant Yudiansyah, Yudiansyah; Ismiyanti, Ismiyanti; Aziz, Sandra Arifin
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.147-154

Abstract

The analysis of pigment content in plants, particularly chlorophyll a, chlorophyll b, anthocyanin, and carotenoids, is crucial to assess their physiological performance. The spectrophotometry method by Sims and Gamon offers practicality and reliable results. This research established several variables to achieve more precise results. Green spinach leaf samples were used as a model to analyze their pigment content, determining the minimum sample area or weight, the maximum limit that does not violate Beer’s law, and the temperature during analysis. The limit of quantitation (LoQ) is the smallest quantity of analytes in a sample that still meets the criteria for accuracy and precision in testing. The research results show that using a leaf punch with a diameter of 9 mm approaches the LoQ value. To avoid measurements nearing the LoQ limit, it is advisable to use leaf punch diameters of 10- or 12-mm. Leaf punches of 22 mm are still safe to use, as there has been no deviationfrom Beer’s law. Using unchilled acetone at 27°C did not show significant differences compared to using cold acetone at 7°C for the content of chlorophyll a, total chlorophyll, and carotenoids. However, for chlorophyll b and anthocyanin, despite significant differences, the chlorophyll content measured at room temperature was more practical and relatively higher compared to cold acetone, thus disproving concerns about pigment damage.
Dynamics of Nutrient Concentrations, Endogenous Hormones, Photosynthetic Capacity, and Phenological Changes in Black Orchid (Coelogyne pandurata Lindl.) from the Vegetative to Generative Phase Heriansyah, Pebra; Aziz, Sandra Arifin; Sukma, Dewi; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.215-234

Abstract

The black orchid (Coelogyne pandurata Lindl.) is one of the native Indonesian orchids from Borneo island. This study analyzed the dynamics of pigments, photosynthetic capacity, endogenous hormones, nutrient concentrations, and phenology across the vegetative to generative stages of the black orchid. The plant materials are one-year-old plants after splitting from the mother plants. Endogenous hormones, nutrient and pigment concentrations, and leaf photosynthetic capacity were measured during vegetative and generative phases. Chlorophyll, anthocyanins, and carotenoids were analyzed using UV-VIS spectrophotometry, Nitrogen (N) was analyzed by the Kjeldahl method, phosphorus (P) and potassium (K) by the Bray 1 method and Flame Photometry, and organic carbon by the Walkley- Black method with titration. The hormone levels were analyzed using HPLC, and photosynthetic capacity was determined using a Li-Cor 6800 system. Phenological changes in leaves, bulbs, and flowers were recorded. Results showed total chlorophyll increased from 1.96 to 2.36 mg/g from the vegetative to the generative stage while carotene slightly declined. Leaf nitrogen rose from 1.54% to 1.70%, bulb nitrogen decreased from 0.67% to 0.45%, whereas the C/N ratio increased from 65.24 to 85.36 from the vegetative to the generative phase. Flower nitrogen was 1.50%, and phosphorus was 0.17%. IAA in leaves decreased from 25.12 to 21.37 ng.g⁻¹ dry weight post-flowering, while gibberellin in bulbs increased from 12.28 to 12.96 ng.g⁻¹ dry weight. Zeatin in bulbs declined from 10.52 to 8.81 ng g⁻¹ dry weight. Photosynthesis peaked at 2.73 μmol. m⁻².s⁻¹ in early generative stages and then declined. Photosynthetic photon flux density and stomatal conductance decreased, reducing water and CO₂ exchange efficiency, while net assimilation and transpiration rates showed no significant changes. These physiological adjustments, including increased chlorophyll levels, changes in nitrogen allocation, and fluctuations in hormone concentrations, reflect the plant’s adaptive strategies to meet higher energy demands during reproductive growth, ensuring efficient resource distribution for flowering.
Co-Authors , Adiwirman , Nazi , Nirwan ,, Herlina . Herlina . TRIVADILA A.A. Ketut Agung Cahyawan W Abdul Munif Agus Yudhi Pardiyanto Andika Akbarul Iman Andria Afrida Anggia Murni Ani Kurniawati Anisya Elsa Shafira Ari, Ahmad Nur Hidayat Gena Arista, Nor Isnaeni Dwi Atang Sutandi Auliya Ilmiawati Ayunina, Krisetya Azmi, Tubagus Kiki Kawakibi Bayuanggara Cahya Ramadhan betty betty Betty Tjhia BUDI MARTONO DADANG DADANG Danar Hadisugelar Dewi Sukma Dhedy Kristanto Didy Sopandie Diny Dinarti DINY DINARTY, DINY Dyah Iswantini Edi Santosa Ekawati, Rina Eman Ayu Sasmita Jati, Gusti Fajar Pangestu Fauziah, Ni'mah Fhonna, Tasya Nurizki Giyanto, dan Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI Haridjaja, Oteng Herlina, NFN Hesti Pujiwati Hesti Pujiwati Hilda Susanti Hilda Susanti I Ketut Suada Ina Kusheryani Ira Fauziah Noer Irmanida Batubara Ismail Saleh Ismail Saleh Ismiyanti, Ismiyanti Isna Tustiyani Isna Tustiyani Jamil, Anna Moslihat Kailola, Joan Joulanda Grace Karimuna, Siti Rahmah Ketty Suketi Krisetya Ayunina Kurniawati, Ari Kurniawati, Ari LATIFAH K. DARUSMAN Leo Mualim M. Rafi MADE ASTAWAN Manalu, Johanes Marojahan MARIA BINTANG Maya Melati Ma’rufah, Siti Hilda Meity Suradji Sinaga Miftahudin . Mohamad Rafi Mufa'adi, Ardianto Muhamad Syukur Muhammad Farid Muhammad Farid Muhammad Syukur Munawaroh, Nafi'atul Munif Ghulamahdi Mustika Tripatmasari Tripatmasari Nafi'atul Munawaroh Neni Musyarofah NFN Herlina Ni'mah Fauziah Noer, Ira Fauziah Nur Faridah, dan Didah Nuri Andarwulan Nurliani Bermawie NURLIANI BERMAWIE NURLIANI BERMAWIE Nurliani Bermawie Nurwita Dewi Oteng Haridjaja Parwito Pebra Heriansyah Purwono Purwono, Purwono Purwono, dan Putri, Astrid Aditya Putri, Fiadini Rahmi Taufika Ramadhan, Bayuanggara Cahya Ramadhani, Elrisa Rasmani, Rasmani Ray March Syahadat Refa Firgiyanto Respita, Intan Annisa rina ekawati Rizva, Dian Novira Sari, Galuh Yunita Shafira, Anisya Elsa Siti Hilda Ma’rufah Siti Maryam Harahap Siti Maryam Harahap Siti Sadiah Slamet Susanto Sri Astuti Rais Stefani Daryanto Sudirman Yahya Sudirman Yahya Tamsin, Aqlia Hanna Nurfatiha Taopik Ridwan Taopik Ridwan Tatik Raisawati Toyip Toyip Tutik Wresdiyati Wahyu Arif Sudarsono Wahyu Arif Sudarsono Waras Nurcholis Wardani, Eska Ayu Winarso D. Widodo Yudiansyah Yudiansyah Yulia Indriani