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All Journal Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Syntax Literate: Jurnal Ilmiah Indonesia Indonesian Journal of Medicine Oto Rhino Laryngologica Indonesiana Majalah Patologi Indonesia Abdimas Universal Ahmar Metakarya: Jurnal Pengabdian Masyarakat The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Plexus Medical Journal
Dyah Ratna Budiani
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Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Nexus Biomedika

 1 
Kombinasi Ekstrak Etanolik Kunyit dan Temulawak Lebih Berpotensi sebagai Fitofarmaka Hepatoprotektif: Studi Efek Hepatoprotektif pada Mencit Balb/C yang Diinduksi Parasetamol Nirmala, Atika Zahro; Widjokongko, S. Bambang; Budiani, Dyah Ratna
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

The occurrence of liver damage especially caused by consuming non steroidal anti inflammatory medicine is high in some countries. In the other hand, turmeric and curcuma are frequently consumed as a herbal treatment. Those herbals contain antioxidant. The purposes of this experiment were to know the differences of hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. This research was a laboratory experimental study with the post test only control group design. The research used 25 male Balb/C mice that were devided into 5 groups: negative [KK (-)] and positive control group [KK (+)] were administered 0.2 ml of aquades, while group treatment 1 (KP1), 2 (KP2) and 3 (KP3) consecutively  were administered 2.8 mg of turmeric ethanolic extract, 14 mg of curcuma ethanolic extract and the combination of 1.4 mg of turmeric extract and 7 mg of curcuma ectract. The treatments were conducted in 14 days. The dose of paracetamol toxic was treated to KK (+), KP1, KP2, and KP3 on the 12th, 13th, and 14th days. Hepatoprotective effect was assessed by the number of disfunctional cells of liver. The data were analyzed by One Way ANOVA and LSD test. The result of one way ANOVA showed a significant difference in the five groups with p = 0.03. LSD test showed a significant difference among KK (-), KP1, and KP3 against KK (+) and KP2. There were some differences of the hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. Keywords: hepatoprotective, turmeric, curcuma, paracetamol consecutively
Pengaruh Ekstrak Air Buah Nanas terhadap Ekspresi Caspase-3 dan Fragmentasi DNA Sel Adenokarsinoma Kolorektal WiDr Pribadi, Sintin Khotijah; Budiani, Dyah Ratna; Pesik, Riza Novierta
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Colorectal adenocarcinoma is the third most diagnosed neoplasm in the world. Many approaches have been taken for therapy, especially targeted therapy. However, the resistance event still occurs due to constitutive activation of intracellular signaling. In the other hand, pineapple fruit [Ananas comosus (L.) Merr.] has bromelain enzyme that can specifically influence intracellular signaling and not only lead to inhibit proliferation but also lead apoptosis induction in neoplasm cell. The purpose of this study was to elucidate the effect of pineapple fruit aqueous extract to caspase-3 expression and DNA fragmentation of apoptosis process in WiDr colorectal adenocarcinoma cells. Methods: This research was an experimental laboratory study with the post test only control group design. The samples of this research were WiDr colorectal adenocarcinoma cells divided into 6 groups: Negative control group, Positive control group with doxorubicin 5 μg/ml and four Treatment groups with 270 μg/ml, 202.5 μg/ml, 135 μg/ml and 67.5 μg/ml of extract concentration, each. The samples were incubated for 48 hours in 37oC and 5% CO2. The caspase-3 expression was determined by cytologic score of samples stained with caspase-3 antihuman antibody. The DNA fragmentation was determined by 1% agarose gel electrophoresis. The cytologic score was analyzed by one way ANOVA test and LSD test. Result: The result showed that there was significant difference between Positive control group and the other groups (p=0.000), but there wasn’t significant difference between the Negative control group and the Treatment groups (p>0.05). The result from electrophoresis showed that there wasn’t DNA fragmentation in WiDr colorectal adenocarcinoma cells. Conclusion: The pineapple fruit aqueous extract did not increase caspase-3 expression and did not lead DNA fragmentation to WiDr colorectal adenocarcinoma cells for 48 hours of treatment. Keywords: pineapple fruit aqueous extract, WiDr colorectal adenocarcinoma cell, caspase-3 expression, DNA fragmentation  
The Correlation between Inflammatory Cells Density and NF-kB Expression in Fibroinflammatory Nasal Polyps Krisnawati Intan Suwignyo; Dyah Ratna Budiani; Fikar Arsyad Hakim
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Asian nasal polyps have more neutrophil-dominant type than Europeans (>50%), the difference of histopathology feature may develope because different etiopathogenesis so they need to treat differently. NF-kB is a transcription factor that plays an important role in corticosteroid resistance mechanism in nasal polyps, while corticosteroid is the first choice of medical treatment for nasal polyps. The aim of this research was to determined the correlation between inflammatory cells density and NF-kB expression in fibroinflammatory nasal polyps. Methods: This analytic observational study had cross-sectional design which was done on 15 paraffin embedded fibroinflammatory nasal polyps tissues from patients who had their first nasal polyps surgery and fixed with buffered formalin. The blocks were taken from Anatomical Pathology Department of RSUD Dr. Moewardi and quota sampling was used to collect the samples. Each samples was stainned using hematoxylin-eosin and NF-kB immunohistochemistry technique, both inflammatory cells and NF-kB nuclear positive were counted, then analyzed using Spearman correlation. Result: Coeficient correlation (r) between lymphocytes and nuclear NF-?B in this study was 0.129 (p = 0.647), plasma cells and nuclear NF-?B was 0.404 (p = 0.135), macrophages and nuclear NF-?B was 0.367 (p = 0.178), while neutrophils and nuclear NF-?B was 0.285 (p = 0.301). Conclusions: There is no significant correlation between inflammatory cells density and NF-?B expression in fibroinflammatory nasal polyps. Key Words: nasal polyps, nuclear NF-kB, inflammatory cells
Pengaruh Ekstrak Air Buah Nanas terhadap Ekspresi Caspase-3 dan Fragmentasi DNA Sel Adenokarsinoma Kolorektal WiDr Sintin Khotijah Pribadi; Dyah Ratna Budiani; Riza Novierta Pesik
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Colorectal adenocarcinoma is the third most diagnosed neoplasm in the world. Many approaches have been taken for therapy, especially targeted therapy. However, the resistance event still occurs due to constitutive activation of intracellular signaling. In the other hand, pineapple fruit [Ananas comosus (L.) Merr.] has bromelain enzyme that can specifically influence intracellular signaling and not only lead to inhibit proliferation but also lead apoptosis induction in neoplasm cell. The purpose of this study was to elucidate the effect of pineapple fruit aqueous extract to caspase-3 expression and DNA fragmentation of apoptosis process in WiDr colorectal adenocarcinoma cells. Methods: This research was an experimental laboratory study with the post test only control group design. The samples of this research were WiDr colorectal adenocarcinoma cells divided into 6 groups: Negative control group, Positive control group with doxorubicin 5 ?g/ml and four Treatment groups with 270 ?g/ml, 202.5 ?g/ml, 135 ?g/ml and 67.5 ?g/ml of extract concentration, each. The samples were incubated for 48 hours in 37oC and 5% CO2. The caspase-3 expression was determined by cytologic score of samples stained with caspase-3 antihuman antibody. The DNA fragmentation was determined by 1% agarose gel electrophoresis. The cytologic score was analyzed by one way ANOVA test and LSD test. Result: The result showed that there was significant difference between Positive control group and the other groups (p=0.000), but there wasnt significant difference between the Negative control group and the Treatment groups (p>0.05). The result from electrophoresis showed that there wasnt DNA fragmentation in WiDr colorectal adenocarcinoma cells. Conclusion: The pineapple fruit aqueous extract did not increase caspase-3 expression and did not lead DNA fragmentation to WiDr colorectal adenocarcinoma cells for 48 hours of treatment. Keywords: pineapple fruit aqueous extract, WiDr colorectal adenocarcinoma cell, caspase-3 expression, DNA fragmentation
Kombinasi Ekstrak Etanolik Kunyit dan Temulawak Lebih Berpotensi sebagai Fitofarmaka Hepatoprotektif: Studi Efek Hepatoprotektif pada Mencit Balb/C yang Diinduksi Parasetamol Atika Zahro Nirmala; S. Bambang Widjokongko; Dyah Ratna Budiani
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The occurrence of liver damage especially caused by consuming non steroidal anti inflammatory medicine is high in some countries. In the other hand, turmeric and curcuma are frequently consumed as a herbal treatment. Those herbals contain antioxidant. The purposes of this experiment were to know the differences of hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. This research was a laboratory experimental study with the post test only control group design. The research used 25 male Balb/C mice that were devided into 5 groups: negative [KK (-)] and positive control group [KK (+)] were administered 0.2 ml of aquades, while group treatment 1 (KP1), 2 (KP2) and 3 (KP3) consecutively were administered 2.8 mg of turmeric ethanolic extract, 14 mg of curcuma ethanolic extract and the combination of 1.4 mg of turmeric extract and 7 mg of curcuma ectract. The treatments were conducted in 14 days. The dose of paracetamol toxic was treated to KK (+), KP1, KP2, and KP3 on the 12th, 13th, and 14th days. Hepatoprotective effect was assessed by the number of disfunctional cells of liver. The data were analyzed by One Way ANOVA and LSD test. The result of one way ANOVA showed a significant difference in the five groups with p = 0.03. LSD test showed a significant difference among KK (-), KP1, and KP3 against KK (+) and KP2. There were some differences of the hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. Keywords: hepatoprotective, turmeric, curcuma, paracetamol consecutively
Co-Authors Alindina Izzani Ambar Mudigdo Andreas Peter Sitorus Andreas Peter Sitorus Ari Natalia Probandari Aris Sudiyanto Arsita Eka Prasetyawati Atika Zahro Nirmala Betty Saptiwi Cempaka Irawati Cempaka Irawati Danus Hermawan Darmawan, Muhammad Dzaki Dhelima, Ismi Cahya Dwi Septiadi Badri Dyonisa Nasirochmi Pakha Eko Dewi Ratna Utami Epistel Pangujian Simatupang Eriana Melinawati Farida Nurhayati Fikar Arsyad Hakim Hakim, Fikar Arsyad Hari Wahyu Nugroho Harsono Salimo Hermawan, Danus Ida Ayu Sinthia Pradnya Swari Ida Nurwati Imam Prabowo Irving, Steven Jarot Subandono Jarot Subandono Krisnawati Intan Suwignyo Lutfi, Sephendra Made Setiamika Mahendra, Fadia Yuda Marcellus Simadibrata Marcellus Simadibrata Martini Martini Melani Ratih Mahanani Muhardjo M Muthmainah Muthmainah Muthmainah Muthmainah Nadzifah Nur Firdaus Ni Kadek Ayu Septiani Sarjana Novan Adi Setyawan Pertiwi, Ina Agustin Rezkita, Bastomy Eka Rifai Hartanto Rino Alvani Gani Rino Alvani Gani Risya Cilmiaty A. R. Riza Novierta Pesik Riza Novierta Pesik S. Bambang Widjokongko Sarsono Sarsono Selfi Handayani Setiawan, Novan Adi Sintin Khotijah Pribadi Sunardo Budi Santoso Sunggoro, Agus Jati Zhahira, Thania Nur