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All Journal Jurnal Gizi dan Pangan Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Statistika Jurnal Natur Indonesia Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology EduChemia: Jurnal Kimia dan Pendidikan ANNALES BOGORIENSES Makara Journal of Science JUARA: JURNAL WAHANA ABDIMAS SEJAHTERA Jurnal Seni dan Reka Rancang: Jurnal Ilmiah Magister Desain E-JRM Jurnal Kedokteran Hewan Kohesi: Jurnal Sains dan Teknologi Annales Bogorienses
Retno Damayanti Soejoedono
Department Of Animal Disease & Veterinary Public Health, Faculty Of Veterinary Medicine, IPB University, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : EduChemia: Jurnal Kimia dan Pendidikan

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Nuclear Magnetic Resonance (NMR) as A Method of Characterizing Antigen Synthesis for Successful Antibody Production Assaat, Lusiani Dewi; Saepudin, Endang; Soejoedono, Retno Damayanti; Adji, Rahmat Setya; Fajriah, Sofa; Ivandini, Tribidasari Anggraningrum
EduChemia: Jurnal Kimia dan Pendidikan Vol 8, No 1 (2023)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/educhemia.v8i1.15572

Abstract

Antibody production is becoming a trend, especially during a pandemic like now, although there have been many developments in antibody production in previous research. Antigen synthesis and characterization is one of the determining factors for the success of antibody production. This research developed a method of antigen characterization using the Nuclear Magnetic Resonance (NMR) method. The research conducted in these 4 stages begins with antigen synthesis, then continues with antigen characterization using NMR, then antibody production, and the last stage is antibody characterization using the Enzyme Linked ImmunoAssay (ELISA) method. The results of antigen characterization using NMR show that NAS has been perfectly conjugated with BSA and produces a NAS-BSA conjugate. This is shown by the presence of 1 methylene (CH2) olefinic at δH chemical shift of 6.27 ppm with an integral of 4 and having 1 piece of methine (CH) sp2 at δH chemical shift of 5.73 ppm with an integral of 2. NAS-BSA antigens have been shown to successfully produce antibodies. This is shown from the anti-NAS-BSA, NAS, and Acrylamide antibodies that begin to appear in the second, third and fourth boosting (respectively)
Co-Authors . Darmawi Achmad Agus Priyono Adji, Rahmat Setya Agus Setiyono Agus Wahyudi Agustin Indrawati Anneke Anggraeni Anugrah Nur Rahmanto Ardiansyah, Romadhony Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Assaat, Lusiani Dewi Ayi Santika Bagas Setya Dian Nugraha Bagus Nanang Luwito Bambang Susilo BIBIANA W LAY Darmawi D Dewi Elfidasari Dwi Desmiyeni Putri Ekowati Handharyani Emilia A Emilia A, Emilia Erick Teguh Leksono Etih Sudarnika Evy Damayanthi Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fajriah, Sofa Fitrianti Rahayu Fitriyana, Intan Nur Hadri Latif Hana Fitria Navratilova Handika Martu Kurniawan Hapsari Mahatmi Harry Tetra Antono I Nyoman Suartha I wayan Teguh Wibawan Irvan Faizal Iskandar, Layla Nurina Kartika Ivandini Tribidasari Anggraningrum Ketut Karuni Nyanakumari Natih Komar Sumantadinata Lilis Suryani M. Lutfi Maggy Thenawidjaja Suhartono Makmur iknu wijaya Mangaraja Pidoli Tampubolon Melani Wahyu Adiningsih Mirnawati Sudarwanto Monika Danaparamitha Andriani Muhammad Hambal Natih, Ketut Karuni Nyanakumari Ni Luh Putu Ika Mayasari Nova Dilla Yanthi Novera Nirmalasanti Nurhidayah Nurhidayah Okti Nadia Poetri R.A. Heryani Wahyuningrum Rahmat Setya Adji Ramlah Ramlah Razali Daud Razali Daud Retno Wijayanti Risa Tiuria Risma Juniarti Silitonga Rizky Alviodinasyari Rudy Ariyanto Saepudin, Endang Sandra Sandra Siti Gusti Ningrum, Siti Gusti sri murtini . Sri Nuryati Suherman . Sukenda . Surachmi Setiyaningsih Sutanti, , Syafril Daulay Syahruddin Said TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Trioso Purnawarman Ummu Balqis Usman Nurhasan Wijaya, Makmur Yuliawati Yuliawati Yuliawati Yus Rusila Noor Yus Rusila Noor Yusuf Hendrawan