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Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC Azzahrah, Putri; Esfandiari, Anita; Mihardi, Arief Purwo; Widhyari, Sus Derthi; Wulansari, Retno; Ningtias, Putri Indah
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.76-87

Abstract

Several pre-analytical variables influence hematological results, including anticoagulant use, storage temperature, and time between blood sample collection and analysis. Delayed sample analysis owing to prolonged storage could result in erythrogram profiles, which could complicate the interpretation of the resulting data. This study investigated the erythrogram profile of tripotassium ethylenediaminetetraacetic acid (K3EDTA) in blood samples stored for 48 h at 4°C. Ten healthy blood samples of Ongole crossbred cattle were collected into K3EDTA tubes from the jugular or coccygeal veins and analyzed for erythrogram profiles (erythrocyte counts, hemoglobin levels, hematocrit value, and erythrocyte morphology). Blood sample analysis for the control (0 h) was performed within ± 1.5 hours after collection, then the samples were refrigerated (4°C) and analyzed at 3, 6, 9, 12, 24, and 48 h. The results showed increased (p < 0.05) erythrocyte counts and hematocrit values after 9–24 and 6–48 h of storage, respectively. There was a significant difference in erythrocyte diameter between 0 h and other time observations (p < 0.05). Echinocytes were observed at 0 h of storage and continued to increase up to 48 h. Hypochromasia was also found at 6 to 48 hours of storage. Therefore, the analysis of blood samples for erythrogram parameters should be performed as soon as possible, preferably within three hours after collection, to ensure clinically reliable results.
Effect of Bosswelic Acid On Hematological Parameters In Sprague Dawley Rats (Rattus novergicus) Induced By Cyclophosphamide Lingga Surya Maret Daulay; Ietje Wientarsih; Anita Esfandiari; Lina Noviyanti Sutardi; Nuzul Asmilia
Asian Journal of Social and Humanities Vol. 2 No. 5 (2024): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v2i5.239

Abstract

Chemotherapy drug “cyclophosphamide (SP)” can trigger oxidative stress that causes hematotoxicity and myelotoxicity, therefore an agent that can suppress the negative effects of cyclophosphamide is needed. Boswellic acid is an herbal medicine that has anti-oxidant and anti-inflammatory effects. The aim of this study was to determine the effect of boswellic acid (BA) in hematology parameter rats induced by cyclophosphamid. Animal were allocated in to six groups, each group consists of 5 rats, the KS: healthy group, KSP: cyclophosphamide group; KSD: comparison group, and BA groups (K1;K2;K3). The treatment groups were given cyclophosphamide (SP) at dose of 150 mg/kg body weight (BW) i.p. The BA groups (K1,K2,K3) were given BA at dose of 250 mg/kg BW orally for 6 consecutive days and SP on day 1 (K1), day 4 (K2), day 6 (K3). The results showed that BA administration (K2 and K3) can help increase/maintain the amount of hematological parameters including hemoglobin, hematocrit, and erythrocytes. Administrating BA for 6 days consecutively before SP (K3) has shown best results on hematological parameters than the others including the comparison group. These results suggest that BA may have a potential herbal therapeutic agents that can mitigate cyclophosphamide-induced hematotoxicity manifestations.
Laporan Kasus: Suspek Hidronefrosis akibat Tumor Prostat pada Anjing di Rumah Sakit Hewan Pendidikan (RSHP) Sekolah Kedokteran Hewan dan Biomedis (SKHB) IPB University Wibowo, Deny Setyo; Wulansari, Retno; Esfandiari, Anita; Widhyari, Sus Derthi; Wijaya, Agus; Gunanti, Gunanti; Fitri, Arni Diana
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.102272

Abstract

Seekor anjing jantan ras domestik berumur 16 tahun dibawa ke Rumah Sakit Hewan Pendidikan (RSHP), Sekolah Kedokteran Hewan dan Biomedis (SKHB), IPB University. Pasien datang dengan keluhan tidak mau makan hingga dua hari, berdiri dan jalan tidak stabil, muntah, nistagmus, dan sempat jatuh terguling. Pemeriksaan fisik palpasi abdomen menunjukkan adanya pembesaran serta nyeri pada kelenjar prostat. Setelah dilakukan pemeriksaan penunjang hematologi, anjing menunjukkan penurunan jumlah eritrosit, hemoglobin, hematokrit, trombosit, dan PCT. Hasil pemeriksaan kimia darah menunjukkan penurunan kadar SGOT serta peningkatan kadar BUN dan kreatinin. Hasil pemeriksaan USG menunjukkan adanya formasi kristal pada pyelum ginjal kiri, perubahan batas antara korteks dengan medula dan atrofi ginjal kanan, peningkatan ketebalan vesika urinaria, serta perubahan ekogenitas parenkim dan ukuran prostat. Hasil pemeriksaan radiografi menunjukkan adanya pembesaran pada prostat dan preputium. Hasil pemeriksaan urinalisis menunjukkan peningkatan UPC serta adanya protein, darah, kreatinin, dan leukosit. Kasus ini memiliki diagnosa banding yaitu hidronefrosis, tumor prostat, prostatitis, dan neuron injury dengan prognosis infausta. Penanganan pada kasus ini dilakukan dengan pemberian terapi berupa infus asering, suplemen, bronkodilatator, diuretik, serta antiemetik.  
Domestic Pigs as an Animal Model of Myocardial Infarction: a Comparison of Systolic and Diastolic Blood Flow Velocities of Cardiac Valves Fitri, Arni Diana; Subangkit, Mawar; Esfandiari, Anita; Gunanti; Noviana, Deni
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.8-15

Abstract

Cardiovascular disease (CVD) is still the leading cause of death according to WHO in 2021. CVD generally results in myocardial infarction (MI), which occurred after tissue ischemia. Echocardiography is most likely a diagnostic tool without invasive effects. This study aimed to compare the blood flow in pig research animals treated with ligases of the circumflex arteries. Measurements were made in the mitral valve (MV), tricuspid valve (TV), semilunar pulmonary valve (SLP), and semilunar aortic valve (AO) of velocity during systole (Vs), velocity during diastole (Vd), time average peak velocity (TAMAX), and blood flow pressure at three-time points: before ligase treatment, after acute infarction (60 min), and 8 weeks after ligase treatment. All parameters in MV group increased at 60 min and decreased at 8th weeks. On TV, TAMAX and pTAMAX decreased at 60 min and week 8. In SLP, VS, pVS, and Vd decreased at 60 min and increased at 8th week. The pVd and pTAMAX parameters decreased at 60 min and 8th week, while TAMAX increased at 60 min and decreased at 8th week. In AO, the TAMAX and pTAMAX parameters increased at 60 min and 8 weeks; the other parameters were similar to MV and TV patterns.
Chronic oral fistula and bladder stones management in a sulcata tortoise (Centrochelys sulcata) Wibowo, Deny Setyo; Esfandiari, Anita; Iman, Bintang Nurul; Rahmiati, Dwi Utari; Fitri, Arni Diana
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.75-76

Abstract

This case study aimed to illustrate the clinical management and therapeutic strategies required for a 4-year-old male Sulcata tortoise named Swift, who presented with significant health issues, including an oral fistula and bladder stones, often indicative of broader husbandry problems. Swift weighed 13 kg and exhibited symptoms such as extensive white lesions across his mouth, an uneven mouth surface, and a foul odour, along with severe lethargy, refusal to eat or drink, and a drooping head. Physical examination revealed dehydration characterised by sunken eyes and reduced skin turgor. Radiographic findings indicated a mass with increased opacity in the mid-body cavity, suggesting the presence of a bladder stone. The treatment involved anaesthesia with ketamine and midazolam, followed by isoflurane gas for maintenance. Surgical interventions included cleaning the oral cavity, inserting a feeding tube, and removing the bladder stone via a transplastron-celiotomy approach. Postoperative care consisted of intracoelomic administration of antibiotics, vitamins, analgesics, and fluid therapy
Citra ultrasonografi dan profil hematologi kasus pyometra pada kucing di Klinik Hewan Cimanggu Soesatyoratih, Rr; Esfandiari, Anita
ARSHI Veterinary Letters Vol. 6 No. 4 (2022): ARSHI Veterinary Letters - November 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.4.61-62

Abstract

Seekor kucing betina bernama Berlin datang ke Klinik Hewan Cimanggu dengan keluhan hewan lesu, kurang nafsu makan dan mengeluarkan cairan merah dari bagian belakang tubuhnya. Hasil pemeriksaan fisik kucing bernama Berlin didapatkan adanya leleran lendir yang berwarna merah keluar dari vulva. Dari hasil anamnesa dan temuan klinis, kucing Berlin diduga menderita pyometra. Pyometra merupakan suatu gangguan reproduksi pada hewan betina yang ditandai dengan adanya penimbunan nanah pada uterus. Dari gejala klinis yang tampak, pyometra yang diderita kucing Berlin adalah pyometra terbuka. Hasil pemeriksaan penunjang ultrasonografi dan pemeriksaan darah terkonfirmasi kucing Berlin menderita pyometra.
Extra-hepatic biliary duct obstruction pada anjing ras Dachshund Kartjito, Nicolas Edward Christanto; Effendi, Siti Aisyah Rahmalia; Solinda, Gamelita Rizkawandi; Widodo, Setyo; Widhyari, Sus Derthi; Wulansari, Retno; Wijaya, Agus; Dewi, Fitriya Nur Annisa; Wibowo, Deny Setyo; Esfandiari, Anita
ARSHI Veterinary Letters Vol. 7 No. 2 (2023): ARSHI Veterinary Letters - Mei 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.2.25-26

Abstract

Anjing jantan ras dachsund (Teckel) berwarna hitam, berusia 4 tahun dengan bobot badan 7,5 kg dibawa pemiliknya datang ke klinik hewan dengan keluhan muntah berulang. Hasil pemeriksaan hematologi menunjukkan polisitemia, leukositosis dan jumlah granulosit yang tinggi. Hasil uji biokimia darah menunjukkan peningkatan pada aktivitas SGPT/ALT, GAMA-GT, total bilirubin, total protein, albumin, rasio ALB/GLOB dan rasio BUN/CRE. Hasil radiografi menunjukkan adanya bentukan membulat radiolucent didekat organ hati. Diagnosa kasus klinis pada anjing ini yaitu extrahepatic biliary duct obstruction dan terapi simptomatik dilakukan dengan memberikan gastroprotektan, antiemetik, serta obat-obatan untuk membantu memperbaiki fungsi hati. Pengobatan dilakukan selama 2 minggu dan menunjukan perbaikan pada organ hati berdasarkan hasil pemeriksaan biokimia darah, diikuti dengan tidak ada dilatasi kantung empedu pada radiogram.
Symmetry dimethylarginine (SDMA) assay for diagnosis of kidney disease in dogs and cats: a mini review of case reports in Indonesia Dewi, Fitriya Nur Annisa; Widhyari, Sus Derthi; Mihardi, Arief Purwo; Widodo, Setyo; Esfandiari, Anita
ARSHI Veterinary Letters Vol. 8 No. 2 (2024): ARSHI Veterinary Letters - May 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.2.35-36

Abstract

Symmetry Dimethylarginine (SDMA) is an important biomarker for early diagnosis of kidney disease. Studies suggest that SDMA is a more sensitive indicator of kidney function compared to traditional markers such as blood urea nitrogen (BUN) and creatinine. Since 2015, the International Renal Interest Society (IRIS) has established SDMA as one of the indicators for diagnosis and determining the stage of chronic kidney disease (CKD) in dogs and cats. SDMA assay use in veterinary clinics in Indonesia has empirically become more common. The aim of this study was to identify scientific reports on SDMA assay use in the diagnosis of kidney disease in dogs and cats in Indonesia. We performed a literature search in the Garuda portal and Google Scholar using the following keywords: “SDMA” or “Symmetry Dimethylarginine”, “dogs”, “cats” and “Indonesia”. The search strategy utilized English and Bahasa Indonesia, and the results were screened for relevance to the topic, and limited to studies conducted in Indonesia. Seven scientific articles in national and international journals reported the use of SDMA assay in cases of kidney disease in dogs or cats in Indonesia, published between 2021-2023. It is expected that SDMA assay use will continue to increase in veterinary practice settings in the country, and it is therefore critical to have a proper understanding of the advantages and limitations of SDMA use for kidney disease diagnosis.
THE EFFECTS OF AGE ON BIOCHEMISTRY PROFILES OF ACEH CATTLE BLOOD Khairita, Lia; Esfandiari, Anita; Widhyari, Sus Derthi; Winarsih, Wiwin
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.21698

Abstract

The purpose of this study was to analyze the effects of age on the biochemistry profiles of aceh cattle, which included examinations of: total protein (TP), albumin, globulin, albumin/globulin ratio (A/G), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), creatine kinase (CK), blood urea nitrogen (BUN), calcium (Ca), phosphorus (P) and magnesium (Mg) . Sixteen clinically healthy aceh cattle aged 2-4 years old, and 16 aceh cattle aged 4-6 years old were purposively selected for the study. Blood samples were drawn from the jugular vein and subjected to blood biochemistry measurements using commercial kits. The results showed that serum concentrations of TP, globulin, AST and Ca of aceh cattle were significantly (P0.05) higher in age group 4-6 years old, while ALP activity was significantly (P0.05) higher in age group 2-4 years old. In conclusion, age must be considered as a factor when interpreting the blood biochemistry profiles of aceh cattle.
Effect of Bosswelic Acid On Hematological Parameters In Sprague Dawley Rats (Rattus novergicus) Induced By Cyclophosphamide Maret Daulay, Lingga Surya; Wientarsih, Ietje; Esfandiari, Anita; Sutardi, Lina Noviyanti; Asmilia, Nuzul
Asian Journal of Social and Humanities Vol. 2 No. 5 (2024): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v2i5.239

Abstract

Chemotherapy drug “cyclophosphamide (SP)” can trigger oxidative stress that causes hematotoxicity and myelotoxicity, therefore an agent that can suppress the negative effects of cyclophosphamide is needed. Boswellic acid is an herbal medicine that has anti-oxidant and anti-inflammatory effects. The aim of this study was to determine the effect of boswellic acid (BA) in hematology parameter rats induced by cyclophosphamid. Animal were allocated in to six groups, each group consists of 5 rats, the KS: healthy group, KSP: cyclophosphamide group; KSD: comparison group, and BA groups (K1;K2;K3). The treatment groups were given cyclophosphamide (SP) at dose of 150 mg/kg body weight (BW) i.p. The BA groups (K1,K2,K3) were given BA at dose of 250 mg/kg BW orally for 6 consecutive days and SP on day 1 (K1), day 4 (K2), day 6 (K3). The results showed that BA administration (K2 and K3) can help increase/maintain the amount of hematological parameters including hemoglobin, hematocrit, and erythrocytes. Administrating BA for 6 days consecutively before SP (K3) has shown best results on hematological parameters than the others including the comparison group. These results suggest that BA may have a potential herbal therapeutic agents that can mitigate cyclophosphamide-induced hematotoxicity manifestations.
Co-Authors . Herlina . Suryono Aditia Dwi Cahyono Agus Lelana Agus Setiyono Agus Wijaya Agus Wijaya Ali Hujarat Amrozi Arief Purwo Mihardi Arif Purwo Mihardi Arifianto, Dinar Azzahrah, Putri Bambang Pontjo Priosoeryanto Bayu Febram Bayu Firmala Kusuma Chusnul Choliq Chusnul Choliq Damiana Rita Ekastuti Darsono Darsono Deni Noviana Deny Setyo Wibowo Dewi, Fitriya Nur Annisa Dondin Sajuthi Dwi Utari Rahmiati Effendi, Siti Aisyah Rahmalia Erni Sulistiawati Fitri, Arni Diana Friska Mery Montolalu Gagah Hendra Wijaya Gunanti Gunanti . Henny Nuraini Huda Shalahudin Darusman I Ketut Sutama I wayan Teguh Wibawan Ida Zahidah Irfan IETJE WIENTARSIH Iman, Bintang Nurul Joan Elviyanti Joesoef, Jayusman Arsiyanti Kartjito, Nicolas Edward Christanto Kathirina Beatrik Riwu Wolo Khairita, Lia Kusumawati, Nina Tri Leni Maylina Lia Khairita Lina Noviyanti Sutardi Lingga Surya Maret Daulay Madyastuti, Rini Maharani Maharani, Maharani Maret Daulay, Lingga Surya Mohamad Yamin Nina Kusumawati Ningtias, Putri Indah Nuzul Asmilia Nuzul Asmilia Retno Wulansari Rizal Arifin Akbari Rizal Rahadian Ramdhany Robby Wienanto Robby Wienanto Safitria Wulandari Setiadi, Hirawan Setyo Widi Nugroho Setyo Widodo Setyo Widodo Soesatyoratih, Rr Solinda, Gamelita Rizkawandi sri murtini . Sri Wahyuni Subangkit, Mawar Suryo Saputro Sus Derthi Widhyai Sus Derthi Widhyari Sus Derthi Widhyarti Tiara Widyaputri Virgilius Martin Kelake Kedang Virgilius Martin Kelake Kedang Wasmen Manalu Wiwin Winarsih Wolo, Kathirina Beathrik Riwu