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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Vektor Penyakit Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Natural Sciences and Mathematics Research BIOMA : Jurnal Biologi Makassar Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia JURNAL KAJIAN VETERINER Jurnal Sylva Lestari Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Indonesian Journal of Jamu
Umi Cahyaningsih
Division Of Parasitology And Medical Entomology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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Molecular Identification of Babesia bigemina from Cattle and Buffaloes in Bogor District Qing Wen, Jyn Soong; Nugraha, Arifin Budiman; Latif, Hadri; Cahyaningsih, Umi
JURNAL KAJIAN VETERINER Vol 12 No 2 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i2.17799

Abstract

Peternakan merupakan industri penting yang memberikan kontribusi signifikan terhadap sektor pertanian di Indonesia. Babesiosis adalah penyakit yang ditularkan melalui vektor caplak dan disebabkan oleh parasit darah genus Babesia. Penyakit ini telah menyebabkan kerugian ekonomi yang sangat signifikan. Deteksi molekular babesiosis memiliki sensitivitas dan spesifisitas yang tinggi. Namun, saat ini, deteksi babesiosis menggunakan Polymerase Chain Reaction (PCR) di Indonesia belum banyak digunaknan, khususnya di Kabupaten Bogor. Tujuan penelitian ini adalah untuk mengidentifikasi Babesia bigemina pada sapi dan kerbau di Kabupaten Bogor dengan teknik molekular. Sebanyak 27 sampel darah dikoleksi yang terdiri atas 22 sampel sapi dan 5 sampel darah kerbau. Sampel diwarnai dengan Giemsa 10%, selain itu sampel darah diekstraksi dan dilanjutkan dengan nested PCR. Hasil dari pemeriksaan apusan darah menunjukkan 81,48% positif terhadap Babesia spp, sedangkan hasil nested PCR 11,11% menunjukkan positif terhadap B. bigemina. Berdasarkan jenis hewan sapi dan kerbau positif terhadap B. bigemina, masing-masing sebesar 9,09% dan 20%. Hasil penelitian ini memberikan informasi dasar mengenai tingkat infeksi Babesia bigemina di kabupaten Bogor berdasarkan metode molekuler. Oleh karena itu, program pencegahan dan pengendalian terhadap infeksi parasit darah pada peternakan sapi dan kerbau sangat perlu dilakukan.
Development of Multiplex PCR for Simultaneous Detection of Trypanosoma evansi and Equine Piroplasma Infection in Horses Nugraha, Arifin; Cahyaningsih, Umi
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.740-746

Abstract

The identification of equine piroplasmosis and surra disease in infected horses currently presents a significant challenge. Clinical symptoms and blood smears are commonly used to diagnose these two diseases. The objective of this study was to develop a multiplex amplification assay capable of simultaneously identifying all three blood protozoa (equine piroplasma and Trypanosoma evansi) in a single test. The primer pairs used for detecting T. equi, B. caballi, and Trypanosoma evansi were ema-2-t (587 bp), Bc-134 (429 bp), and Rotat 1.2 VSG (151 bp), respectively. The multiplex PCR assay was subsequently evaluated for its detection limit, sensitivity percentage, and specificity using single PCR as the reference standard. The multiplex PCR method demonstrated a sensitivity of 100% for detecting both Theileria equi and Trypanosoma evansi, with all positive samples confirmed by a single PCR. It also achieved 100% specificity for both the parasites. However, for Babesia caballi, while the sensitivity remained at 100%, the specificity was reduced to 66%, indicating some limitations in accurately identifying negative samples. For the multiplex PCR assay, the minimum detectable concentrations were 0.01 ng/µL for both T. equi and B. caballi, whereas Trypanosoma evansi exhibited a detection threshold of 1 ng/µL. When multiplex PCR assays were used to screen blood samples obtained from horses in selected districts of Bogor, only a single sample showed a positive result for T. equi. The sensitivities and specificities achieved by multiplex PCR and single PCR were comparable. Therefore, the multiplex PCR method developed in this study can be effectively applied to diagnose piroplasmosis and surra in horses.
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Deteksi molekuler Babesia spp. menggunakan PCR pada anjing dari Bogor dan Jakarta, Indonesia Veerasamy, Suganti; Nugraha, Arifin Budiman; Cahyaningsih, Umi; Fitri, Arni Diana; Satyawardana, Wirokartiko
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.73-74

Abstract

Babesiosis pada anjing disebabkan oleh infeksi Babesia spp.; namun, metode deteksinya terbatas di Indonesia. Penelitian ini bertujuan untuk mendeteksi Babesia spp. pada anjing dari Bogor dan Jakarta melalui PCR. Sampel darah (n=56) dikumpulkan dan diperiksa menggunakan apusan darah dengan pewarnaan Giemsa 10% dan PCR yang menargetkan gen 18S rRNA. Profil hematologi juga dianalisis. Hasil penelitian menunjukkan bahwa 12,5% anjing ras campuran dari Bogor positif terinfeksi Babesia spp. melalui kedua metode. Anjing yang terinfeksi menunjukkan jumlah sel darah merah yang berkurang dan jumlah sel darah putih yang meningkat, yang mengindikasikan anemia dan respons imun. Penelitian ini menyoroti pentingnya metode molekuler untuk diagnosis yang akurat dan mendukung perlunya program pencegahan dan pengendalian.
Dynamics of intraerythrocytic parasite infections in the Java common palm civet (Paradoxurus hermaphroditus) Putri, Maritrana; Cahyaningsih, Umi; Ekastuti, Damiana Rita; Tarigan, Ronald; Achmadi, Pudji; Bustamam, Isdoni; Santoso, Koekoeh; Suprayogi, Agik; Maheshwari, Hera; Satyaningtijas, Aryani
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.93-94

Abstract

Selama tujuh minggu, penelitian ini menyelidiki infeksi parasit intraeritrosit pada musang Jawa (Paradoxurus hermaphroditus). Sampel darah dikumpulkan segera setelah musang dibawa dari habitat aslinya. Setelah periode adaptasi 30 hari, sampel tambahan dikumpulkan setiap minggu. Temuan kami mengungkapkan infeksi dengan Babesia sp., Theileria sp., dan Anaplasma sp., dengan tingkat infeksi tertinggi untuk Anaplasma sp. (0,35 ± 0,05)%, diikuti oleh Theileria sp. (0,12 ± 0,05)%, dan Babesia sp. (0,03 ± 0,02)%. Selama periode penelitian, tingkat infeksi Babesia sp. dan Anaplasma sp. menurun, sedangkan Theileria sp. menunjukkan tren peningkatan hingga akhir penelitian.
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Ekawasti, Fitrine; Azmi, Zul; Subekti, Didik Tulus; desem, muhammad ibrahim; Nugraha, Arifin Budiman; Sadiah, Siti; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Yuniarto, Ichwan; Subekti, Didik T; Cahyaningsih, Umi; Satrija, Fadjar
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.11486

Abstract

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (Prevalence and Risk Factor of Coccidiosis in Dairy Cattle in Bandung District) Sufi, Isrok Malikus; Cahyaningsih, Umi; Sudarnika, Etih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Saridewi, Rismayani; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2824

Abstract

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA Mirsageri, M.; Assidiqi, M. Jamaluddin; Cahyaningsih, Umi; Tiuria, Risa; Z, Zulfiqri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
Co-Authors A.S. Satyaningtijas Agik Suprayogi Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arini Ratnasari ASEP SAEFUDDIN Azizah, Hanifah Nur Azmi, Zul Budi Santosa Budi Santosa Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Fitri, Arni Diana Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hadri Latif HERA MAHESHWARI Ichwan Yuniarto Inarsih, Dwi Isdoni Bustaman Isrok Malikus Sufi Jodi Vanden Eng koekoeh santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Mahdi Jufri MIRNA WATI DEWI Mirnawati Sudarwanto MIRNAWATI SUDARWANTO MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem MULYATI EFFENDI Nepho, Farlin Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Nuradji, Harimurti Nurcahyo, Raden Wisnu Nurridho Wahid, Muhammad Pudji Achmadi Purwantiningsih Sugita Purwanto, Eko Setyo Putra, Eliyus Putri, Maritrana Qing Wen, Jyn Soong RESSY RIANDCI Ridi Arif Rini Damayanti Risa Tiuria Rismayani Saridewi Rismayani Saridewi, Rismayani Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Roza Sadiah, Siti Satyawardana, Wirokartiko Sa’diah , Siti Sa’diah, Siti Siti Sa'diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Sufi, Isrok Malikus Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Tarigan, Ronald Tjahja Muhandri Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Winarsongko, Agus Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Yulia Yellita Yuniarto, Ichwan Zul Azmi Zulfiqri Z Zulfiqri Z, Zulfiqri