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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Vektor Penyakit Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Natural Sciences and Mathematics Research BIOMA : Jurnal Biologi Makassar ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia JURNAL KAJIAN VETERINER Jurnal Sylva Lestari Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Indonesian Journal of Jamu
Umi Cahyaningsih
Division Of Parasitology And Medical Entomology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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Pendampingan Pengeringan Tanaman Obat dengan Tipe Fluidized Bed Dryer untuk Usaha Mikro, Kecil dan Menengah di Kota Bogor Umi Cahyaningsih; Tjahja Muhandri; Arifin Nugraha
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 2 (2023): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.9.2.242-247

Abstract

Drying with the fluidized bed dryer type is a drying process by blowing hot air from the bottom onto a stretch of material, so that the material scatters to resemble fluid properties. The drying method with dry air is used to speed up the drying process and maintain the quality of the dry ingredients. The purpose of this service is to provide theoretical and practical drying training to the micro, small and medium enterprises (MSME) group so that they are able to produce dry materials of good quality, namely green color and low water content so they are not easily damaged and can last longer. The assistance was carried out for Naphyco MSME partners and representatives of RW 10 residents, Tanah Baru Village, North Bogor District, Bogor City in October 2022 at Naphyco MSME partner locations. The training was carried out using two methods, namely presentation and hands-on practice of drying medicinal plants with a fluidized bed dryer. The positive impact of this assistance is to gain knowledge about how to dry medicinal plants faster than drying them in direct sunlight, and also to obtain leaves of medicinal plants that are green in color (not brown). The achievement of mentoring activities can be seen from the results of the pre-test and post-test. The results of the questionnaire showed an increase in participant knowledge from a good score of 55.6% to 88.9%, a moderate score from 22.2% to 0% and a value less than 22.2% to 11.1%. There was also an increase in participants' interest in the questionnaire statements who strongly agreed from 66.7 to 88.9%, who agreed from 33.3% to 11.1%. The results of the questionnaire above need to be continued with a mentoring program for other MSME members to produce quality products as raw materials.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (Prevalence and Risk Factor of Coccidiosis in Dairy Cattle in Bandung District) Isrok Malikus Sufi; Umi Cahyaningsih; Etih Sudarnika
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA M. Mirsageri; M. Jamaluddin Assidiqi; Umi Cahyaningsih; Risa Tiuria; Zulfiqri Z
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Ichwan Yuniarto; Didik T Subekti; Umi Cahyaningsih; Fadjar Satrija
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.11486

Abstract

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia.
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Fitrine Ekawasti; Zul Azmi; Didik Tulus Subekti; muhammad ibrahim desem; Arifin Budiman Nugraha; Siti Sa’diah; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that  stored on liquid nitrogen was isolated using DNAzol™ reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Rismayani Saridewi; Denny Widaya Lukman; Mirnawati Sudarwanto; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2824

Abstract

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 °C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan  susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.
Ultrasound-Assisted Extraction of Antimalarial Compounds from the Bidara Laut (Strychnos ligustrina) Heartwood Sri Familasari; Rita Kartika Sari; Wasrin Syafii; Anne Carolina; Umi Cahyaningsih; Siti Sa'diah
Jurnal Sylva Lestari Vol. 11 No. 3 (2023): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v11i3.682

Abstract

The aqueous wood extract of bidara laut (Strychnos ligustrina) from maceration demonstrated the greatest antimalarial activity against Plasmodium falciparum strain 3D7. However, this method has several limitations, including a lengthy extraction time, which allows for microorganism contamination. The aim of this study was to quantify the yield, evaluate the antimalarial effectiveness of the P. falciparum growth in-vitro, and analyze the aqueous bidara laut heartwood extracts phytochemical composition from the two different extraction methods,  namely the method of maceration extraction for 24 hours and the ultrasound-assisted extraction (UAE) for 30 (UAE30), 45 (UAE45), and 60 (UAE60) minutes. The bidara laut heartwood extract from the UAE60 method had relatively similar yield and antimalarial activity with the bidara laut heartwood extract from the UAE45 method. However, these extracts had higher yield and antimalarial activity than the extracts from the maceration and the UAE30 methods. The results of LC-MS/MS analysis revealed that the main compounds in the UAE45 and UAE60 extracts were strychnine, quinaprilat, and 3,4-bis-cyclobut-3-ene-1,2-dione, which has potent antimalarial properties. Keywords: antimalarial activity, Plasmodium falciparum, quinaprilat, strychnine, Strychnos ligustrina
Parasit Gastrointestinal pada Kadal Naga Berjanggut (Pogona vitticeps) di Cijeruk, Bogor Nisrina Rosyida Noor Rifai; Arifin Budiman Nugraha; Ekowati Handharyani; Fajar Kawitan; Umi Cahyaningsih
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.80391

Abstract

Bearded dragons are reptiles that are quite popular as pets. There are many factors of disease that can infect bearded dragons. The environment and feed affect the level of disease threat, such as parasitic infection in the gastrointestinal tract. Data information related to parasitic gastrointestinal infections in bearded dragons in Indonesia is still limited, so supporting examinations are necessary to help diagnose. A total of 38 fecal samples were collected from the bearded dragon in Cijeruk Bogor. Samples were examined by simple flotation method, Ziehl Neelsen staining, and counting the number of parasitic eggs. All samples were positive for gastrointestinal parasite infections such as Choleoeimeria sp., Isospora amphiboluri, Cryptosporidium spp., ascarid egg, and oxyurid egg. The average number of Choleoeimeria sp. and Isospora amphiboluri oocysts were 8730 and 51.281 oocysts per gram of feces. The average number of helminth eggs per gram was 290 and 2325 for ascarid and oxyurid, respectively. Most of the infected bearded dragons do not show significant clinical symptoms. The number of oocysts and worm eggs per gram of feces showed mild and high results.
Leukocyte Profile of Mice Infected with Plasmodium berghei and Treated with Aqueous Extract of Strychnos Ligustrina and Its Combination with Dihydroartemisinin Piperaquine Phosphate (DHP) Umi Cahyaningsih; Siti Sa'diah; Wasrin Syafii; Rita Kartika Sari; Arifin Budiman Nugraha
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.88896

Abstract

Malaria is still one of the most highly pathogenic diseases, which remains a significant problem for public health. Natural compounds are alternative ways as an antimalarial compound that are applied for the artemisinin combination. The aim of this study was to determine the effect of a combination of S. ligustrina extract and DHP on the differential leukocytes percentages. This study used 100 mice which were divided into five groups. Group A and B were used for the healthy mice and infected-untreated groups, respectively. Group C used as a controlled drug, received 222 mg/kg BW of DHP. Group D received 300 mg/kg BW of aqueous extract of S. ligustrina. Group E received a combination between 111 mg/kg BW of DHP and 200 mg/kg BW of aqueous extract S. ligustrina. The findings demonstrated that DHP and S. ligustrina extract had an impact on neutrophils, lymphocytes, inhibition, and percentages of parasitemia. The findings revealed that treatment groups C, D, and E reduced the percentage of parasitemia. The percentage of neutrophils in treated groups was significantly higher than in group A (P<0.05). The percentage of lymphocytes was lower in the treated groups, while the percentages of eosinophils, monocytes, and basophils did not significantly different (P<0.05). This study found that group D and E had the same effect on the leucocyte profile as group C. Our findings suggest that the aqueous extract of S. ligustrina, as well as the combination treatment with DHP, have the potential to lead to the discovery of new antimalarial medicines.
Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii Fitrine Ekawasti; Agus Winarsongko; Farlin Nepho; Eko Setyo Purwanto; Didik Tulus Subekti; harimurti nuradji; NLP Indi Dharmayanti; Riza Zainuddin Ahmad; Siti Sa’diah; Umi Cahyaningsih; Raden Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90867

Abstract

AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
Co-Authors A.S. Satyaningtijas Agik Suprayogi Agus Winarsongko Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arifin Nugraha Arifin Nugraha Arini Ratnasari ASEP SAEFUDDIN Azizah, Hanifah Nur Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Farlin Nepho Fitri, Arni Diana Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fong, Sulinawati Hadri Latif harimurti nuradji HERA MAHESHWARI Ichwan Yuniarto Isdoni Bustaman Isrok Malikus Sufi Isrok Malikus Sufi Jodi Vanden Eng koekoeh santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Mirsageri Mahdi Jufri MIRNA WATI DEWI Mirnawati Sudarwanto MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem Muhammad Nurridho Wahid MULYATI EFFENDI Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Pudji Achmadi Purwantiningsih Sugita Putri, Maritrana Qing Wen, Jyn Soong Raden Wisnu Nurcahyo RESSY RIANDCI Rini Damayanti Risa Tiuria Rismayani Saridewi Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Satyawardana, Wirokartiko Siti Sa&#039;diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Tarigan, Ronald Tjahja Muhandri Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Zul Azmi Zulfiqri Z