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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Vektor Penyakit Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Natural Sciences and Mathematics Research BIOMA : Jurnal Biologi Makassar ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia JURNAL KAJIAN VETERINER Jurnal Sylva Lestari Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Indonesian Journal of Jamu
Umi Cahyaningsih
Division Of Parasitology And Medical Entomology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Ekawasti, Fitrine; Azmi, Zul; Subekti, Didik Tulus; desem, muhammad ibrahim; Nugraha, Arifin Budiman; Sadiah, Siti; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Yuniarto, Ichwan; Subekti, Didik T; Cahyaningsih, Umi; Satrija, Fadjar
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.11486

Abstract

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (Prevalence and Risk Factor of Coccidiosis in Dairy Cattle in Bandung District) Sufi, Isrok Malikus; Cahyaningsih, Umi; Sudarnika, Etih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Saridewi, Rismayani; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2824

Abstract

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA Mirsageri, M.; Assidiqi, M. Jamaluddin; Cahyaningsih, Umi; Tiuria, Risa; Z, Zulfiqri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
Pemberian Ekstrak Air Bidara Laut (Strychnos Ligustrina) terhadap Diferensial Leukosit Mencit yang diinfeksi dengan Plasmodium berghei Nurridho Wahid, Muhammad; Cahyaningsih, Umi; Nugraha, Arifin
Jurnal Veteriner dan Biomedis Vol. 2 No. 1 (2024): Maret
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.2.1.35-42.

Abstract

Plasmodium berghei merupakan hemoprotozoa penyebab penyakit malaria pada mencit. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak air bidara laut terhadap gambaran diferensial leukosit pada mencit yang diinfeksi Plasmodium berghei. Penelitian ini menggunakan 49 ekor mencit Balb/c, dibagi menjadi 7 kelompok yaitu kontrol normal (KN), kontrol infeksi tanpa perlakuan (KI), kontrol infeksi yang diberikan perlakuan obat kombinasi dihydroartemisinin 25 mg/kgbb dan piperakuin fosfat dosis 197 mg/kgbb (K0), EAa (ekstrak aquades), EAb (ekstrak aquades : DHF = 1:1), EAc (ekstrak aquades : DHF = 1:2), EAd (ekstrak aquades : DHF = 2:1). Kecuali KN, semua kelompok mencit diinfeksi Plasmodium berghei sebanyak 1 x 10 6 /ml secara intraperitoneal. Setiap hari diambil darah dari ekor mencit, dibuat apusan tipis dan dilakukan pewarnaan dengan Giemsa 10%, kemudian dilakukan perhitungan diferensial leukosit. Nilai rata-rata persentase diferensial leukosit diolah menggunakan uji ANOVA dan perbedaan hasil persentase pada masing-masing kelompok diketahui dengan menggunakan uji Duncan menggunakan software SPSS. Hasil penelitian menunjukkan bahwa perlakuan pada kelompok EAa dan EAd mengalami penurunan persentase neutrofil dan meningkatkan persentase limfosit pada hari ke 6 setelah infeksi. Persentase monosit, eosinofil dan basofil tidak mengalami perubahan yang signifikan. Pengobatan ekstrak air bidara laut dan DHF berpotensi sebagai obat antimalaria dengan menurunkan tingkat parasitemia dan meningkatkan persentase limfosit.
Perbandingan Ekstrak Jahe Merah (Zingiber Officinale Roscoe. Var. Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro Sa’diah , Siti; Anwar, Effionora; Jufri, Mahdi; Cahyaningsih, Umi
Jurnal Jamu Indonesia Vol. 4 No. 3 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i3.160

Abstract

Metode ekstraksi dan jenis pelarut yang digunakan akan berdampak pada proses penarikan komponen aktif sehingga akan berpengaruh juga pada aktivitasnya. Jahe merah (Zingiber officinale var. Rubrum) merupakan salah satu bahan rempah yang juga berpotensi sebagai tanaman obat. Salah satu khasiat ekstrak jahe merah adalah sebagai anti toksoplasma. Pada penelitian ini telah dilakukan pembuatan ekstrak jahe merah dengan dua jenis metode yaitu maserasi dan sokhletasi masing-masing menggunakan tiga jenis pelarut (etanol 30%, etanol 70% dan etanol 96%) sehingga diperoleh enam jenis ekstrak. Kemudian masing-masing ekstrak ditentukan kadar senyawa pencirinya meliputi 6-gingerol, 8-gingerol, 10-gingerol dan 6-shogaol dengan Kromatografi Cair Kinerja Tinggi (KCKT). Selanjutnya ekstrak diuji aktivitas antitoksoplasma secara in vitro terhadap parasit Toksoplasma gondii pada fase takhizoit yang ditumbuhkan pada sel vero. Penentuan aktivitas antitoksoplasma juga dilakukan pada senyawa murni 6, 8, 10-gingerol dan 6-shogaol serta kontrol positif antibiotik spiramisin. Hasilnya menunjukkan bahwa ekstrak etanol 96% dengan metode maserasi adalah yang paling baik aktivitasnya dan 6-gingerol adalah senyawa penciri yang paling berperan sebagai anti-toksoplasma.
Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii Ekawasti, Fitrine; Winarsongko, Agus; Nepho, Farlin; Purwanto, Eko Setyo; Subekti, Didik Tulus; nuradji, harimurti; Dharmayanti, NLP Indi; Ahmad, Riza Zainuddin; Sa’diah, Siti; Cahyaningsih, Umi; Nurcahyo, Raden Wisnu
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90867

Abstract

AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
The The Pattern of Biological Character, Morphometry, and Genetic Information of Trypanosoma evansi Isolated from Bogor District, Indonesia Nugraha, Arifin Budiman; cahyaningsih, umi
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.24299

Abstract

Trypanosoma evansi (T. evansi) merupakan penyebab utama trypanosomiasis pada hewan ternak. Penelitian ini dilakukan untuk menggambarkan variasi virulensi, morfometri, dan karakter genetik empat isolat T. evansi yang berasal dari sapi dan kerbau di Kabupaten Bogor, Jawa Barat. Seluruh isolat diperbanyak pada mencit untuk mengamati periode prepatent, perkembangan parasitemia, ciri morfologi, serta hubungan genetiknya. Hasil penelitian menunjukkan adanya tiga pola virulensi. Isolat dengan virulensi sedang berasal dari sapi dan kerbau menyebabkan kematian mencit pada hari ke-14 pascainfeksi. Dua isolat lain dari sapi menunjukkan virulensi rendah (kematian pada hari ke-24) dan virulensi tinggi (hari ke-4). Perbedaan ukuran tubuh juga terlihat jelas, masing-masing 22,05 µm pada isolat virulensi rendah, 24,1 µm pada virulensi sedang, dan 24,6 µm pada virulensi tinggi. Analisis sekuensing menunjukkan bahwa isolat dengan virulensi rendah dan sedang berada dalam kelompok yang sama, sedangkan isolat virulensi tinggi berkelompok dengan isolat dari Thailand dan Tiongkok. Studi ini menunjukkan bahwa isolat T. evansi di lapangan memiliki keragaman biologis dan genetik yang cukup lebar, sehingga pemantauan terhadap penyebarannya tetap diperlukan, termasuk pada isolat yang tampak kurang virulen.
Eimeria Species Composition and Factors Influencing Oocysts Shedding in Dairy Farm, Bandung, Indonesia Sufi, Isrok Malikus; Cahyaningsih, Umi; Sudarnika, Etih
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.28 KB) | DOI: 10.11598/btb.2017.24.2.516

Abstract

Coccidiosis is one of the most widespread parasitic diseases affecting cattle worldwide, caused by Eimeria spp. This study aimed to determine the composition of Eimeria species and the factors influencing Eimeria oocyst shedding in dairy farms. A cross-sectional study was conducted in dairy farms in South Bandung District from July 2014 to January 2015. Fecal samples were collected from 400 dairy cattle, consisting of 196 calves aged < 6 months, 37 cattle aged 6–12 months, and 167 cattle aged > 12 months. Samples were examined and quantified for Eimeria species composition and oocyst per gram (OPG) counts using the McMaster technique. A questionnaire was administered to dairy farmers to gather information on cattle health status and husbandry practices. The effects of sex, age, and pen flooring type on OPG values were analyzed using Mann–Whitney and Kruskal–Wallis tests, followed by Dunn’s multiple comparison test. Ten Eimeria species were identified in the infected cattle. Among them, Eimeria bovis showed the highest prevalence (42.5%), followed by Eimeria wyomingensis (39.1%), Eimeria bukidnonensis (32.4%), Eimeria pellita (26.3%), Eimeria auburnensis (19.6%), Eimeria zuernii (17.3%), Eimeria cylindrica (3.9%), Eimeria canadensis (3.9%), Eimeria brasiliensis (3.4%), and Eimeria alabamensis (1.1%). Oocyst shedding was significantly correlated (p < 0.05) with cattle sex, age, and pen flooring type, all of which influenced infection pressure. Younger calves aged < 6 months shed the highest numbers of Eimeria oocysts compared to older cattle. Several factors may contribute to the increased OPG in fecal samples; therefore, maintaining proper sanitation and implementing effective Eimeria control measures are essential for dairy cattle management in KPBS Pangalengan.
Co-Authors A.S. Satyaningtijas Agik Suprayogi Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arifin Nugraha Arini Ratnasari ASEP SAEFUDDIN Azizah, Hanifah Nur Azmi, Zul Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Fitri, Arni Diana Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hadri Latif HERA MAHESHWARI Ichwan Yuniarto Isdoni Bustaman Isrok Malikus Sufi Jodi Vanden Eng koekoeh santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Mahdi Jufri MIRNA WATI DEWI Mirnawati Sudarwanto MIRNAWATI SUDARWANTO MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem MULYATI EFFENDI Nepho, Farlin Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Nuradji, Harimurti Nurcahyo, Raden Wisnu Nurridho Wahid, Muhammad Pudji Achmadi Purwantiningsih Sugita Purwanto, Eko Setyo Putri, Maritrana Qing Wen, Jyn Soong RESSY RIANDCI Rini Damayanti Risa Tiuria Rismayani Saridewi Rismayani Saridewi, Rismayani Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Sadiah, Siti Satyawardana, Wirokartiko Sa’diah , Siti Sa’diah, Siti Siti Sa&#039;diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Sufi, Isrok Malikus Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Tarigan, Ronald Tjahja Muhandri Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Winarsongko, Agus Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Yuniarto, Ichwan Zul Azmi Zulfiqri Z Zulfiqri Z, Zulfiqri