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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Vektor Penyakit Journal of Natural Sciences and Mathematics Research BIOMA : Jurnal Biologi Makassar ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia JURNAL KAJIAN VETERINER Jurnal Sylva Lestari Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Indonesian Journal of Jamu
Umi Cahyaningsih
Division Of Parasitology And Medical Entomology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii Ekawasti, Fitrine; Winarsongko, Agus; Nepho, Farlin; Purwanto, Eko Setyo; Subekti, Didik Tulus; nuradji, harimurti; Dharmayanti, NLP Indi; Ahmad, Riza Zainuddin; Sa’diah, Siti; Cahyaningsih, Umi; Nurcahyo, Raden Wisnu
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90867

Abstract

AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
Deteksi molekuler Babesia spp. menggunakan PCR pada anjing dari Bogor dan Jakarta, Indonesia Suganti Veerasamy; Arifin Budiman Nugraha; Umi Cahyaningsih; Arni Diana Fitri; Wirokartiko Satyawardana
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.73-74

Abstract

Babesiosis pada anjing disebabkan oleh infeksi Babesia spp.; namun, metode deteksinya terbatas di Indonesia. Penelitian ini bertujuan untuk mendeteksi Babesia spp. pada anjing dari Bogor dan Jakarta melalui PCR. Sampel darah (n=56) dikumpulkan dan diperiksa menggunakan apusan darah dengan pewarnaan Giemsa 10% dan PCR yang menargetkan gen 18S rRNA. Profil hematologi juga dianalisis. Hasil penelitian menunjukkan bahwa 12,5% anjing ras campuran dari Bogor positif terinfeksi Babesia spp. melalui kedua metode. Anjing yang terinfeksi menunjukkan jumlah sel darah merah yang berkurang dan jumlah sel darah putih yang meningkat, yang mengindikasikan anemia dan respons imun. Penelitian ini menyoroti pentingnya metode molekuler untuk diagnosis yang akurat dan mendukung perlunya program pencegahan dan pengendalian.
Dynamics of intraerythrocytic parasite infections in the Java common palm civet (Paradoxurus hermaphroditus) Maritrana Putri; Umi Cahyaningsih; Damiana Rita Ekastuti; Ronald Tarigan; Pudji Achmadi; Isdoni Bustamam; Koekoeh Santoso; Agik Suprayogi; Hera Maheshwari; Aryani Satyaningtijas
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.93-94

Abstract

Selama tujuh minggu, penelitian ini menyelidiki infeksi parasit intraeritrosit pada musang Jawa (Paradoxurus hermaphroditus). Sampel darah dikumpulkan segera setelah musang dibawa dari habitat aslinya. Setelah periode adaptasi 30 hari, sampel tambahan dikumpulkan setiap minggu. Temuan kami mengungkapkan infeksi dengan Babesia sp., Theileria sp., dan Anaplasma sp., dengan tingkat infeksi tertinggi untuk Anaplasma sp. (0,35 ± 0,05)%, diikuti oleh Theileria sp. (0,12 ± 0,05)%, dan Babesia sp. (0,03 ± 0,02)%. Selama periode penelitian, tingkat infeksi Babesia sp. dan Anaplasma sp. menurun, sedangkan Theileria sp. menunjukkan tren peningkatan hingga akhir penelitian.
Co-Authors A.S. Satyaningtijas Agik Suprayogi Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arifin Budiman Nugraha Arini Ratnasari Arni Diana Fitri Aryani Satyaningtijas ASEP SAEFUDDIN Azizah, Hanifah Nur Azmi, Zul Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hadri Latif HERA MAHESHWARI Ichwan Yuniarto Isdoni Bustaman Isrok Malikus Sufi Jodi Vanden Eng Koekoeh Santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Mahdi Jufri Maritrana Putri MIRNA WATI DEWI Mirnawati Sudarwanto MIRNAWATI SUDARWANTO MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem MULYATI EFFENDI Nepho, Farlin Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Nuradji, Harimurti Nurcahyo, Raden Wisnu Nurridho Wahid, Muhammad Pudji Achmadi Purwantiningsih Sugita Purwanto, Eko Setyo Qing Wen, Jyn Soong RESSY RIANDCI Rini Damayanti Risa Tiuria Rismayani Saridewi Rismayani Saridewi, Rismayani Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Ronald Tarigan Sadiah, Siti Sa’diah , Siti Sa’diah, Siti Siti Sa'diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Sufi, Isrok Malikus Suganti Veerasamy Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Winarsongko, Agus Wirokartiko Satyawardana Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Yuniarto, Ichwan Zul Azmi Zulfiqri Z Zulfiqri Z, Zulfiqri