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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Silvikultur Tropika (JST) Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Techno: Jurnal Penelitian Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Kultivasi Jurnal Agrotek Tropika Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat

Supramana

Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Kamper, Kampus IPB, Darmaga, Bogor 16680

Author-ID : 2452045

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences

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Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa Aprilyani Aprilyani; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia.

Tingkat Infestasi Aphelenchoides besseyi pada Benih Padi di Bogor Fitrianingrum Kurniawati; Supramana Supramana
Jurnal Fitopatologi Indonesia Vol 12 No 1 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The white tip disease on rice plants have been discovered in the district of Bogor, i.e. in Sukamakmur, Petir, and Muara Experimental Station areas. The infected plant exhibited symptoms, involving whiten leaf tip 3–5 cm that later turn to necrosis, twisted and crinkled. The disease is supposed caused by a parasitic nematode, namely Aphelenchoides besseyi, which is known seed borne. Extraction of nematodes based on ISTA protocols on rice was conducted. Seeds from eight varieties, SL8SHS, Hybrid Rice (HIPA14), IPB 3S, IR-64, Pertiwi (Pak Tiwi), Inpari 31, Pandan Wangi Bogor (Sintanur) and Ciherang, was obtained from seed vendors in Bogor and Muara Experimental Station. The majority of nematodes recovered were females and only few males with morphological characteristics matching with A. besseyi. All seed rice varieties tested contained A. besseyi with the average of 3–341 nematodes per 5 g seed.

Bakteri Endofit Asal Berbagai Akar Tanaman sebagai Agens Pengendali Nematoda Puru Akar Meloidogyne incognita pada Tomat Pradana Pandu Ankardiansyah; Abdul Munif; Supramana Supramana
Jurnal Fitopatologi Indonesia Vol 12 No 3 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Infection caused by root knot nematode (RKN) Meloidogyne incognita may cause yield losses. Little is known regarding the effectiveness of endophytic bacterial group as biocontrol agents of RKN. This research was aimed to obtain endophytic bacteria group from 16 species of plants, which effectively controlled the RKN. Isolation of endophytic bacteria group was conducted using NA 20%, NA 50%, TSA 20%, TSA 50%, and King’s B medium. All of the bacteria groups giving negative result in hypersensitive and haemolytic tests, was further examined for their ability to produce protease, chitinase, and cyanide acid. The same endophytic bacteria groups were also tested for their potential to control juvenile 2 of M. incognita on tomatoes by seed treatment and soil drenching. Agronomical and pathological traits were observed 40 days after nematodes infestation. Eighty endophytic bacteria groups were successfully isolated and 17 of them were considered potential. Physiological test showed that 16 groups of endophytic bacteria can produce protease enzyme, 12 groups can produce chitinase enzyme, and 5 groups can produce cyanide acid. Specific endophytic bacteria group, i.e. TmtN5 from roots of tomato plant, is the most effective isolate for suppressing root damage and population of RKN. This group was effective as biocontrol agents of RKN because it produceds chitinase, protease, and cyanide acid. This research provided a new information regarding the potential use of endophytic bacteria group as a biocontrol agent of RKN.

Deteksi dan Identifikasi Ditylenchus dipsaci dari Umbi Bawang Putih Impor Elmi Muliya; Supramana Supramana; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Detection and Identification of Ditylenchus dipsaci from Imported Garlic BulbsImportation rate of garlic (Allium sativum) increased significantly in Indonesia lately. This situation may lead to the introduction and spread of quarantine pests, one of them is bulbborne nematode Ditylenchus dipsaci. This research is aimed to detect and identify the presence of D. dipsaci on imported garlic bulb from China. Samples of garlic bulbs were taken from imported garlic through Tanjung Priok Sea Port, North Jakarta, within importation period from August to December 2017. Nematode extraction was performed by modified Baermann funnel method. Nematode identification was carried out based on the D. dipsaci key morphological characters and morphometry according to de Man formula. This nematode was successfully detected from 20% of garlic bulbs samples based on morphological characteristics, i.e. slender body shape, body length between 0.56 until 1 mm, well-defined stylet (10-13 mm in length), posterior valve between oesophagus and intestine is not overlapping, median bulb obvious with oval shape, and the tail was conoid with a pointed terminus. The findings of D. dipsaci is an important fact because these nematodes have the potential to cause damage to plants in the field.

Keefektifan Perlakuan Air Panas terhadap Nematoda Ditylenchus destructor pada Umbi Bawang Putih Heri Ahmadi; Supramana Supramana; Mohamad Rahmad Suhartanto
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The Effectiveness of Hot Water Treatment Against Nematode Ditylenchus destructor on Garlic BulbsThe high importation of garlic increases the risk of entry and spread of Ditylenchus to Indonesia. The hot water treatment (HWT) has the potential to be developed as an effective and safe method for elimination Ditylenchus in garlic. The aim of the current research was to examine effectiveness of HWT application on imported garlic to eliminated Ditylenchus. The research consisted of garlic sampling, nematode identification and determination of population abundance, and HWT. Samples were taken from traditional markets. The range of HWT temperature tested was 41, 43, 45, 47, 49, 51, 53, and 55 °C for 20 minutes and control. Optimization of treatment time was carried out at 49–51 °C for 5, 10, 15, 20, 25, 30 minutes and control. The results showed that based on morphological characters the parasitic nematodes that infect imported garlic from China were D. destructor. Nematode populations are varied in the sample, the highest number was 508 nematodes per 50 g of garlic. Hot water temperature at 41–51 °C did not affect the quality of garlic, and the temperature of 49–55 °C caused 100% nematode mortality. Hot water at 49 °C for 20–30 minutes or 51 °C for 20–25 minutes effectively eliminated Ditylenchus in garlic without affecting the garlic quality. The research confirmed the presence of D. destructor in imported garlic imported from China, so it is recommended to tighten inspection at a port of entry and monitoring its potential spread in Indonesia.

Morfologi dan Morfometri Nematoda Sista Kentang (Globodera spp.) Asal Dataran Tinggi Dieng, Jawa Tengah Auliya Selamet; Supramana Supramana; Meity Suradji Sinaga; Ali Nurmansyah; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 15 No 2 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The Morphology and Morphometry of Potato Cyst Nematodes (Globodera spp.) from Dataran Tinggi Dieng, Central JavaIn Indonesia, the potato cyst nematode (PCN) was first reported in 2003, at potato plantation in Batu, East Java. Golden cyst (Globodera rostochiensis) was detected in Bandung, West Java; Banjarnegara, Central Java; Batu, East Java; and Gowa, South Sulawesi, whereas the pale cyst (G. pallida) has been reported at limited distribution in Dataran Tinggi Dieng (Central Java). The aim of this research was to identify the Globodera species of Dataran Tinggi Dieng based on morphological and morphometrical methods. Soil samples were collected from Dieng plateau at 30 selected potato plantations ranging from 1 100 – 2 100 m above sea level. The identification of PCN was done by observing the morphological and morphometrical key characters of cysts and second juveniles (J2). Two species of Globodera, that are G. rostochiensis and G. pallida, were identified from soil samples. The key characters of G. pallida including distinct forward projection of J2 stylet knob, granek’s ratio value less than 3, and number of cuticular ridges between vulval basin and anus are less than 12 were identified. There were 25 specimens that having granek’s ratio less than 3, and 10 specimens having cuticular ridges number less than 12. G. rostochiensis which has the shape of a stylet knob protrudes posteriorly and the value of the granek’s ratio greater than 3.5 was identified in 30 specimens.

Potential of Bacterial Isolates from Peat Land as Controlling Agent for the Root Knot Nematodes Meloidogyne incognita Elvina Efendi; Supramana; Giyanto
Jurnal Fitopatologi Indonesia Vol 17 No 6 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.6.243-250

Potential of Bacterial Isolates from Peat Land as Controlling Agent for the Root Knot Nematodes Meloidogyne incognita The root-knot nematodes (Meloidogyne spp.) is an important parasite of cultivated plants and have a wide host range. One of the potential alternative to controlling this pathogen is by utilizing non-pathogenic bacteria. This study aims to evaluate the potential of bacteria from peat as a biological control agent for the root-knot nematodes (M. incognita). In vitro testing was conducted by using filtrate of 15 bacterial isolates against M. incognita juvenile 2 (J2) in a 5 cm petri dish. Fivety juveniles (J2) of M. incognita were added to 4.5 mL of bacterial filtrate and incubated at 27 ℃. Nematode mortality was observed at 6, 12, and 24 hours after treatment. The experiment was arranged in a completely randomized design with three replications. The variables observed include the percentage of nematode mortality and the length of lethal exposure to nematodes. Physiological characterization test was carried out to the bacterial isolates including the production of HCN and chitinase enzymes. The results showed that nine bacterial isolates, that are GA2 GAA1, GT1 GTA7, GT1 GTB3, GT1 GTB4, GT1 GTB6, GT1 GTB7, GT1 GTC2, GT1 GTC4, and STDHC4 perform nematicidal activities with mortality level 83% to 94%. Three bacterial isolates, that are GT1 GTB4, GT1 GTB7, and GT1 GTC2 produce chitinase enzymes with lysis index above 1, but none of those isolates produce HCN. Bacterial isolates GT1 GTB4 and GT1 GTB7 had 99% homology with Serratia marcescens from China, and GT1 GTC2 had 99% homology with Streptomyces sp. AT67 from South Korea.

EFFICACY OF ENDOPHYTIC BACTERIA IN REDUCING PLANT PARASITIC NEMATODE Pratylenchus brachyurus Rita Harni; Supramana Supramana; Supriadi Supriadi
Indonesian Journal of Agricultural Science Vol 15, No 1 (2014): April 2014
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v15n1.2014.p29-34

Pratylenchus brachyurus is a major parasitic nematode on patchouli that reduces plant production up to 85%. The use of endophytic bacteria is promising for controlling nematode and promoting plant growth through production of phytohormones and enhancing the availability of soil nutrients. The objective of the study was to evaluate the efficacy of endophytic bacteria to control P. brachyurus on patchouli plant and its influence on plant productions (plant fresh weight and patchouli oil). The study was conducted at Cimanggu Experimental Garden and Laboratory of the Indonesian Spice and Medicinal Crops Research Institute (ISMECRI), Bogor, West Java. The experi-ment was designed in a randomized block with seven treatments and eight replications; each replication consisted of 10 plants. The treatments evaluated were five isolates of endophytic bacteria (Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK and Bacillus subtilis NJ57), synthetic nematicide as a reference, and non-treated plant as a control. Four-week old patchouli plants of cv. Sidikalang were treated by soaking the roots in suspension of endophytic bacteria (109 cfu ml-1) for one hour before trans-planting to the field. At one month after planting, the plants were drenched with the bacterial suspension as much as 100 ml per plant. The results showed that applications of the endophytic bacteria could suppress the nematode populations (52.8-80%) and increased plant weight (23.62-57.48%) compared to the control. The isolate of endophytic bacterium Achromobacter xylosoxidans TT2 was the best and comparable with carbofuran.

PENGARUH FILTRAT BAKTERI ENDOFIT TERHADAP MORTALITAS, PENETASAN TELUR DAN POPULASI NEMATODA PELUKA AKAR Pratylenchus brachyurus PADA NILAM RITA HARNI; SUPRAMANA SUPRAMANA; MEITY S. SINAGA; GIYANTO GIYANTO; SUPRIADI SUPRIADI
Jurnal Penelitian Tanaman Industri Vol 16, No 1 (2010): Maret 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v16n1.2010.43-47

ABSTRAKPratylenchus brachyurus merupakan salah satu patogen utama padatanaman nilam di Indonesia. Pengendalian yang banyak dilakukan petanisaat ini adalah menggunakan pestisida sintetik. Penggunaan pestisidasintetik yang terus menerus merupakan ancaman terhadap lingkungan, dankesehatan manusia. Bakteri endofit mungkin dapat dimanfaatkan sebagaisalah satu teknik pengendalian nematoda yang ramah lingkungan karenabakteri endofit dapat menghasilkan racun yang toksik terhadap nematoda.Tujuan penelitian adalah melihat pengaruh kultur filtrat bakteri endofitterhadap mortalitas nematoda, penetasan telur dan perkembangannematoda di dalam akar nilam. Penelitian dilakukan di Laboratorium danRumah kaca Hama dan Penyakit Balai Penelitian Tanaman Obat danAromatik Bogor, dari bulan Januari sampai April 2008 menggunakanrancangan acak lengkap (RAL). Filtrat bakteri dibuat dengan caramenumbuhkan bakteri endofit pada media TSB selama 48 jam, kemudiandisentrifugasi dengan kecepatan 7.000 rpm selama 15 menit. Filtratdisaring dengan milipore berdiameter 0,22 µm, selanjutnya filtrat diujipada nematoda in vitro dan rumah kaca. Hasil penelitian menunjukkanbahwa filtrat dapat membunuh nematoda dalam waktu 24 jam dengannilai LC 50 sebesar 7,709%. Bakteri endofit isolat TT2 dan EH11memperlihatkan daya bunuh paling tinggi yaitu 91-100%. Di samping itufiltrat bakteri endofit juga dapat menekan penetasan telur nematoda 48,5-74,6% dibanding dengan kontrol. Namun hanya filtrat bakteri endofitisolat EH11 yang nyata dapat menekan populasi nematoda di dalam akarnilam dengan tingkat penekanan sebesar 81,3%.Kata kunci : Pratylenchus brachyurus, bakteri endofit, kultur filtrat,Pogostemon cablinABSTRACTEffect of culture filtrates endophytic bacteria on themortality, hatching eggs and population of root lesionnematodes Pratylenchus brachyurus on patchouliRoot lesion nematode (Pratylenchus brachyurus) is an importantpathogen of patchouli in Indonesia and causes significant losses. Controlsystem that are done today is using synthetic pesticides. The use ofsynthetic pesticides is a continuing threat to the environment and humanhealth. However, endophytic bacterial culture filtrates may be used as oneof the nematode control that is environmentally friendly. Effect of culturefiltrates endophytic bacteria on the mortality, hatching eggs and populationroot lesion nematodes Pratylenchus brachyurus on patchouli has beendone in vitro and greenhouse. The results showed that the culture filtrate ofendophytic bacteria produced metabolite toxic to nematodes and wereable to kill P. brachyurus 100% within 24 hours with LC 50 7.709%. TT2and EH11 isolates showed high killing power of 91-100%. The culturefiltrates also inhibited hatching of P. brachyurus eggs compared withcontrols. Not all culture filtrates can suppress the nematode population inthe roots of patchouli. EH11 isolates filtrate really pressing nematodepopulations compared to other isolates.Key words: Pratylenchus brachyurus, culture filtrate, endophyticbacteria, Pogostemon cablin

MEKANISME BAKTERI ENDOFIT MENGENDALIKAN NEMATODA Pratylenchus brachyurus PADA TANAMAN NILAM Rita Harni; Supramana Supramana; Meity S. Sinaga; Giyanto Giyanto; Supriadi Supriadi
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 1 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n1.2012.%p

Beberapa jenis bakteri endofit telah diketahui potensinya sebagai agens hayati terhadap nematoda parasit P. brachyurus pada tanaman nilam. Penelitian dilakukan untuk mengetahui mekanisme pengendalian dari beberapa bakteri endofit terhadap P. brachyurus pada tanaman nilam. Setek nilam berumur satu bulan diperlakukan dengan bakteri endofit Achromobacter xylosoxidans TT2, Bacillus subtilis NJ57, Alcaligenes faecalis NJ16, Bacillus cereus MSK, dan Pseudomonas putida EH11 dengan metode split root system (sebagi-an akar diinokulasi dengan bakteri endofit (populasi 109/pot), dan bagian lainnya diinokulasi dengan P. brachyurus (100 ekor/pot). Penelitian mengunakan rancangan acak lengkap (RAL) enam per-lakuan dengan tujuh ulangan. Peng-amatan dilakukan terhadap populasi nematoda yang mempenetrasi akar, kadar asam salisilat, fenol, dan peroksidase. Kadar asam salisilat, fenol, indol acetic acid dan peroksidase pada tanaman dianalisis menggunakan metode HPLC dan spektrofotometer. Hasil penelitian menunjukkan bahwa meka-nisme kerja bakteri endofit dalam mengendalikan P. brachyurus adalah dengan menginduksi ketahanan tanaman dengan peningkatan produksi senyawa kimia penginduksi ketahanan seperti asam salisilat, peroksidase dan fenol oleh bakteri endofit A. xylosoxidans TT2, A. faecalis NJ16 dan P. putida EH11. Di samping itu, bakteri endofit juga dapat memicu pertumbuhan tanaman melalui peningkatan indole acetic acid terutama pada perlakuan dengan Bacillus cereus MSK. Hasil penelitian mengindikasikan bahwa aplikasi beragam jenis bakteri endofit yang berbeda mekanisme kerja-nya tersebut perlu dilakukan secara ber-samaan untuk mendapatkan pengendali-an yang optimal.

Co-Authors . Giyanto Abdul Muin Adnan ABDUL MUNIF Abdul Munif ABDUL MUNIF Ade Indra Maulana Sembiring ALI NURMANSYAH Anindita, Devina Cinantya Ankardiansyah Pandu Pradana Annisa Nur Imamah Aprilyani Aprilyani Aris Budiman Aris Budiman Auliya Selamet Efi Toding Tondok Elmi Muliya Elvina Efendi Fitrianingrum Kurniawati, Fitrianingrum GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Giyanto GIYANTO GIYANTO Giyanto Giyanto GIYANTO GIYANTO Halimah Halimah Hamidi, Ilmi HARNI, RITA Heri Ahmadi Heriyanto Syafutra Hermanu Triwidodo Hishar Mirsam Ibrahim, Ahmad Yusuf IKA MUSTIKA IKA MUSTIKA Imamah, Annisa Nur Indahsari, Rizkhi Kikin H Mutaqin Lisnawita Lisnawita Lisnawita Lufthi Rusniarsyah MEITY S. SINAGA Meity S. Sinaga MEITY S. SINAGA Meity Suradji Sinaga Mohamad Rahmad Suhartanto Mokhammad Danang Kusuma Muhamad Taher Muhammad Jabal Nur MUSTIKA, IKA Pradana Pandu Ankardiansyah RAUF, AUNU RITA HARNI RITA HARNI Rita Harni RITA HARNI RITA HARNI RITA HARNI Salsabila, Unik Hanifah Samsudin . Sembiring, Ade Indra Maulana Sitanggang, Della SRI HENDRASTUTI HIDAYAT SUPRIADI SUPRIADI Supriadi Supriadi Supriadi Supriadi SUPRIADI SUPRIADI Syarifah Syarifah, Syarifah TRI ASMIRA DAMAYANTI Utami, Budi Sri Victoria Coo Lea Wawan Kurniawan Widodo Widodo Yundari, Yundari Zalzilatul Hikmia Hikmia

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