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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Silvikultur Tropika (JST) Agrikultura Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Techno: Jurnal Penelitian Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Kultivasi Jurnal Agrotek Tropika Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Biodiversitas Journal of Biological Diversity
Supramana Supramana
Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, IPB Dramaga Campus, Bogor, West Java 16680 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences

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Evaluation of thermotherapy on potato tubers to control tuber-borne nematodes, Meloidogyne spp. Kurniawan, Wawan; Supramana, Supramana; Munif, Abdul; Giyanto, Giyanto
Kultivasi Vol 24, No 1 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i1.62705

Abstract

Root knot nematodes (Meloidogyne spp.) are significant plant-parasitic nematodes frequently transmitted through potato tubers and are a major factor contributing to the decline in both crop quality and yield. This study aims to evaluate the effectiveness of thermotherapy for eliminating Meloidogyne spp. from potato tubers and its impact on sprouting viability. Thermotherapy was conducted by immersing potato tubers and second-stage juveniles (J2) of Meloidogyne spp. in water at temperatures of 50 °C, 52.5 °C, 55 °C, and 60 °C for durations ranging from 5 to 75 minutes, depending on the treatment. Parameters observed included the mortality rate of Meloidogyne spp. J2 and the growth viability of potato seeds. The results showed that thermotherapy applied to second-stage juveniles (J2) of Meloidogyne spp. at 50 °C for 20 minutes resulted in complete (100%) nematode mortality. Similarly, the application of thermotherapy to potato tubers at 50 °C for 40 minutes did not significantly affect seed viability. During this treatment, the internal temperature of the tubers, measured at a depth of 1.5 cm, reached the target temperature of 50 °C at the 20-minute mark and was maintained until the 40th minute, ensuring an effective thermal exposure. These results indicate that thermotherapy at 50 °C for 40 minutes represents a safe and effective method for the elimination of Meloidogyne spp. in potato tubers. This approach offers a practical and promising strategy to enhance seed health and minimize the risk of nematode dissemination in potato cultivation systems. 
Ketahanan Varietas Padi terhadap Nematoda Pucuk Putih Aphelenchoides besseyi: The Resistance of Rice Varieties Against White Tip Nematode Aphelenchoides besseyi Imamah, Annisa Nur; Supramana, Supramana; Damayanti, Tri Asmira
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.7-17

Abstract

Penyakit pucuk putih pada padi yang disebabkan oleh Aphelenchoides besseyi merupakan penyakit tergolong baru dan telah tersebar di Indonesia. Penggunaan varietas tahan merupakan salah satu cara terbaik untuk mengendalikan A. besseyi. Namun, belum ada informasi terkait ketahanan varietas padi terhadap A. besseyi di Indonesia. Sembilan varietas padi diuji respons ketahanannya terhadap A. besseyi dengan menggunakan inokulum sebanyak 500 ekor nematoda per tanaman. Nematoda A. besseyi diinfestasikan pada fase pembungaan dengan cara menyemprotkan suspensi nematoda ke bagian malai dan diamati panjang malai, jumlah bulir, jumlah bulir bernas dan hampa, berat 100 bulir bernas, jumlah nematoda, dan ekspresi gen PBZ1 padi. Deteksi gen ketahanan PBZ1 dilakukan dengan one step reverse transcription-polymerase chain reaction (RT-PCR) menggunakan primer spesifik PBZ1F dan PBZ1R. Berdasarkan parameter hasil panen, populasi A. besseyi, dan ekspresi gen PBZ1, respons ketahanan varietas padi terhadap infestasi A. besseyi dibagi menjadi tiga kategori ketahanan, yaitu tahan (‘Ciherang’, ‘Utri Merah’, dan ‘Utri Rajapan’), toleran (‘Inpago 7’, ‘IPB 3S’, ‘Prima’, dan ‘Situ Bagendit’), dan rentan (‘Inpara 3’ dan ‘Inpara 4’). Infestasi A. besseyi menginduksi ekspresi gen PBZ1 pada varietas uji dengan intensitas berbeda tergantung tingkat ketahanan varietas. Varietas ‘Ciherang’, ‘Inpago 7’, ‘Situ Bagendit’, ‘Utri Merah’, dan ‘Utri Rajapan’ menunjukkan ekspresi gen PBZ1 tinggi baik sebelum maupun setelah infestasi A. besseyi. Intensitas ekspresi gen PBZ1 yang tinggi sebelum infestasi A. besseyi menunjukkan varietas ini merupakan sumber potensial varietas tahan terhadap A. besseyi. Hasil penelitian menunjukkan ada korelasi antara tingginya ekspresi gen PBZ1 dengan ketahanan varietas padi terhadap A. besseyi.
Keefektifan Tumbuhan Rorippa indica sebagai Biofumigan untuk Pengendalian Nematoda Puru Akar pada Tanaman Mentimun: Effectiveness of Rorippa indica as a Biofumigant for Controlling Root Knot Nematodes on Cucumber Plants Bramasta, Muhammad Willy; Supramana; Abdul Munif
Jurnal Fitopatologi Indonesia Vol. 20 No. 6 (2024): November 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.6.295-304

Abstract

Meloidogyne spp. merupakan nematoda parasit penting pada berbagai tanaman yang menyebabkan gejala penyakit seperti puru akar, kerdil, daun menguning, dan tanaman menjadi layu. Nematisida botani memiliki potensi menjanjikan sebagai metode pengendalian nematoda. Penelitian ini bertujuan mengetahui potensi Rorippa indica sebagai biofumigan dalam mengendalikan nematoda puru akar (Meloidogyne incognita). Percobaan biofumigasi dilakukan melalui 2 tahapan, yaitu percobaan laboratorium dan rumah kaca dengan masa inkubasi selama 14 hari untuk proses biofumigasi. Percobaan di laboratorium menggunakan polibag berisi 100 g tanah yang diinfestasi 100 juvenil 2 (J2) M. incognita sebelum perlakuan biofumigasi. Potongan batang, daun, dan seluruh bagian tumbuhan R. indica dicampurkan pada polibag dengan bobot bahan 1.3, 2.3, dan 3.9 g sebagai perlakuan. Percobaan di rumah kaca dilakukan dengan menambahkan potongan R. indica dengan bobot bahan 150 g per polibag yang berisi 3 kg tanah dan telah diinfestasi 300 J2 M. incognita. Tanaman mentimun berumur 7 hari setelah semai dipindahkan ke dalam polibag yang telah dibiofumigasi dan diamati selama 6 minggu. Hasil pengujian menunjukkan bahwa biofumigasi menggunakan R. indica dalam skala laboratorium terbukti efektif mengurangi populasi nematoda sebesar 87%, sedangkan pada skala rumah kaca menyebabkan perbedaan pembentukan puru yang signifikan dibandingkan perlakuan kontrol. Hal ini berkaitan dengan hasil analisis GC-MS yang menunjukkan kandungan terpenoid pada ekstrak R. indica. Perlakuan R. indica dapat meningkatkan pertumbuhan tanaman mentimun dibandingkan kontrol.
Efektivitas Gelombang Ultrasonik dalam Eliminasi Nematoda Daun Aphelenchoides pada Umbi Bawang Merah Kurniawati, Fitrianingrum; Supramana, Supramana; Hidayat, Sri Hendrastuti; Tondok, Efi Toding; Syafutra, Heriyanto; Damayanti, Tri Asmira; Mubin, Nadzirum
Jurnal Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68192

Abstract

Nematoda daun Aphelenchoides merupakan salah satu patogen tanaman yang memiliki kisaran inang yang luas dan menyebabkan kehilangan hasil panen. Metode konvensional telah sering digunakan dalam pengendalian nematoda termasuk nematisida kimia, perawatan air panas, dan nematisida hayati tetapi memiliki keterbatasan. Hal ini memerlukan eksplorasi metode alternatif yang ramah lingkungan dan efektif, seperti teknologi gelombang ultrasonik. Penelitian bertujuan mengetahui efektivitas gelombang ultrasonik dalam eliminasi nematoda daun Aphelenchoides pada umbi bawang serta pengaruh pada pertumbuhan umbi bawang. Ekstraksi nematoda menggunakan metode pengabutan (mist chamber) dan perendaman air dingin. Nematoda diidentifikasi berdasarkan karakter morfologi. Umbi bawang merah yang telah diinfestasikan Aphelenchoides diberi perlakuan ultrasonik dengan lama perlakuan selama 26, 30, 37, 44, 60 menit dan kontrol. Umbi bawang yang telah diberi perlakuan ultrasonik ditanam di pot dan diamati parameter pertumbuhannya sampai panen. Data ditabulasikan di Microsoft Excel dan diolah menggunakan SAS JMP. Perlakuan ultrasonik dengan durasi yang lebih dari 37 menit menghasilkan tingkat mortalitas yang tinggi (≥84,00%).  Waktu papar yang berbeda menunjukkan pengaruh yang tidak berbeda nyata dengan kontrol pada tinggi tanaman dan panjang akar. Lebih lanjut, perlakuan ultrasonik dengan durasi yang lebih lama (≥ 37 menit) sangat efektif dalam menekan populasi nematoda Aphelenchoides tanpa memengaruhi pertumbuhan tanaman bawang merah.
Identifikasi Spesies Aphelenchoides pada Benih Padi Varietas Lokal Sumatera Utara Berdasarkan Marka Molekuler Della Sitanggang; Supramana; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 22 No. 1 (2026): Maret 2026: IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.22.1.7-11

Abstract

The nematode Aphelenchoides besseyi was successfully detected and morphologically identified in the rice seeds of Samosir local varieties, namely ‘Siserang’, ‘Sipining’, ‘Sibandung’, and ‘Saratus Ari’. This study aimed to confirm the identity of Aphelenchoides species using universal primers as markers for the D2-D3 expansion region of the 28S rDNA gene. Nematodes were extracted from the seeds of the North Sumatra local varieties including ‘Siserang’, ‘Sipining’, ‘Sipala’, ‘Sirias’, ‘Sigambiri Merah’, and ‘Sigambiri Putih’. Nematodes from the commercial rice variety ‘Pak Tiwi-1’ was used for comparison. DNA extraction of A. besseyi nematodes was carried out using the Holterman method. The amplification process used universal primers D2A-D3B, producing a DNA fragment of approximately 750 bp. DNA amplification of A. besseyi from the Samosir, Simalungun, and Pakpak Bharat Districts successfully produced DNA fragments of approximately 750 bp. Nematodes obtained from the Siserang, Sigambiri Merah, Sirias, Sigambiri Putih, Sipala, and Sipining varieties showed 99.8%–100% homology with A. besseyi isolates from the Indonesia commercial rice variety, Japan, Brazil, and China.
Ultrasonication for the Control of Aphelenchoides besseyi in Local Rice Seed Varieties from North Sumatra Della Sitanggang; Supramana Supramana; Tri Asmira Damayanti
Jurnal Perlindungan Tanaman Indonesia Vol 30, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.107062

Abstract

Aphelenchoides besseyi is one of the most important plant parasitic nematode in rice causing the white tip disease. Its dormant phase is seed-borne, making rice seeds a medium for the long-distance dispersal of this nematode. This study aimed to assess the potential of ultrasonic wave application as a seed treatment method for eliminating A. besseyi nematodes from rice seeds. The seed varieties tested were six local varieties from North Sumatra namely Siserang, Sipining, Sipala, Sirias, Sigambiri Merah, and Sigambiri Putih. The commercial rice variety Pak Tiwi-1 was used as a comparison. Seed treatment was conducted with an ultrasonic cleaner unit at 40 kHz for 5, 10, 15, 20, 25, and 30 minutes, respectively.  Ultrasonic exposure at 40 kHz for 20 and 30 minutes were able to eliminate A. besseyi by 100%, resulting in 0% incidence of white tip disease in seedlings. Ultrasonic seed treatment shows potential as an effective, practical, and relatively fast technique for eliminating A. besseyi from rice seeds. 
Nematode diversity in rice fields of Java, Indonesia FITRIANINGRUM KURNIAWATI; SUPRAMANA SUPRAMANA; R. YAYI MUNARA KUSUMAH; DHIVA SYAFA QUAMILLA; SOBIKHIN SOBIKHIN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261228

Abstract

Abstract. Kurniawati F, Supramana, Kusumah RYM, Quamilla DS, Sobikhin. 2025. Nematode diversity in rice fields of Java, Indonesia. Biodiversitas 26: 6273-6284. Rice (Oryza sativa) is a staple crop in Indonesia; however, its productivity is constrained by plant-parasitic nematodes that cause substantial yield losses. This study investigates the diversity and abundance of nematodes in soil and rice roots across Java Island. Soil and root samples were purposively selected based on symptoms of nematode infection, and nematodes were extracted and identified morphologically. Ecological indicators, including Maturity Index family and diversity metrics (H’, E, D), were quantified to evaluate food web structure and soil ecosystem conditions using Microsoft Excel and the NINJA platform. Plant-parasitic nematodes were widely distributed, with Hirschmanniella and Meloidogyne dominating the assemblage, reflecting strong herbivory pressure in flooded rice systems. Free-living nematodes were predominantly bacterivores, indicating a bacterial-driven decomposition pathway typical of anaerobic paddy soils. Maturity Index (MI) values (2.65-3.07) suggested moderate and relatively uniform disturbance across regions, although ΣMI varied significantly, with higher values in Bogor and Malang indicating more mature soil food webs. Diversity indices showed that Malang and Sukabumi exhibited the highest ecological stability, whereas Banten and Bojonegoro displayed low diversity and high dominance, characteristic of stressed environments. The consistently high Enrichment Index (EI) and Structure Index (SI) values indicated active nutrient cycling and resilient trophic structures. Overall, Java’s rice ecosystems experience moderate disturbance yet maintain functionally robust nematode communities, underscoring the need for region-specific management, particularly in high-Plant Parasitic Index (PPI) areas, to improve soil health and mitigate nematode-associated yield losses.
First record of the spiral nematode Scutellonema brachyurum (Rhabditida: Hoplolaimidae) in strawberry plants Fitrianingrum Kurniawati; Supramana Supramana; Dhiva Quamilla
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.126208-215

Abstract

Strawberries are a popular fruit crop with promising cultivation prospects in Indonesia. However, their production is constrained by various pathogens, including plant-parasitic nematodes. This study aimed to characterize the nematode Scutellonema brachyurum infecting strawberry plants in West Java using morphological and molecular approaches. Soil and root samples were selectively collected from both symptomatic and asymptomatic plants. Nematodes were extracted from soil using the flotation–centrifugation technique and from roots using the mist chamber method. Morphological identification was complemented by molecular analysis using universal D2A/D3B primers targeting the D2–D3 region of the 28S rRNA gene, followed by DNA sequencing. Data were processed and analyzed descriptively. The nematode S. brachyurum infecting strawberry plants was successfully characterized using both morphological and molecular techniques. PCR amplification produced DNA fragments of approximately 700 bp. Nucleotide sequence analysis revealed that S. brachyurum isolates from West Java shared 83.7%–93.8% similarity with isolates from other countries. Scutellonema brachyurum was identified in Lebakmuncang (Ciwidey Sub-district) and Alamendah (Rancabali Sub-district), Bandung Regency, as well as in Langensari (Lembang Sub-district), West Bandung Regency. The nematode population detected in the samples ranged from 1 to 6 individuals. These findings indicate that S. brachyurum is distributed across several major strawberry-producing areas in West Java.
Identification of Meloidogyne spp. (Tylenchida: Meloidogynidae) as potato tuber-borne nematodes (PTBN) in the potato production center of Java Island, Indonesia Wawan Kurniawan; Supramana Supramana; Giyanto Giyanto; Abdul Munif
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 2 (2026): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.226473-484

Abstract

Root-knot nematodes (Meloidogyne spp.) are among the most destructive plant-parasitic nematodes affecting potato production worldwide. However, information on Meloidogyne spp. associated with potato tubers and their potential as potato tuber-borne nematodes (PTBN) in Indonesia remains limited. This study aimed to identify Meloidogyne species associated with potato tubers and determine their distribution in major potato-producing regions of Java, Indonesia. Surveys were conducted from January 2023 to December 2024 in West, Central, and East Java. Symptomatic potato tubers were collected and examined using morphological, morphometric, and molecular approaches. Tubers were stored under refrigerated conditions for 3–24 months to evaluate nematode persistence. Molecular identification was performed using species-specific SCAR markers, followed by phylogenetic and sequence homology analyses. Root-knot nematodes were detected in all surveyed production areas and were associated with characteristic symptoms, including knobby, branched, elongated, and russeted tubers. Two species were identified: Meloidogyne incognita, which was widely distributed throughout Java, and Meloidogyne javanica, which was detected only in Cikandang Village, Garut, West Java. The nucleotide sequences were deposited in GenBank under accession numbers LC894939–LC894951 (M. incognita) and LC894966 (M. javanica). Both species remained viable in potato tubers for up to 24 months, confirming their potential as PTBN. These findings provide a scientific basis for strengthening nematode-free seed certification and phytosanitary measures to reduce the spread of Meloidogyne spp. through seed tubers.
Co-Authors . Giyanto Abdul Muin Adnan Abdul Munif ABDUL MUNIF ABDUL MUNIF Ade Indra Maulana Sembiring Ali Nurmansyah Anindita, Devina Cinantya Ankardiansyah Pandu Pradana Annisa Nur Imamah Aprilyani Aprilyani Aris Budiman Aris Budiman Auliya Selamet Bramasta, Muhammad Willy Della Sitanggang Della Sitanggang Dhiva Quamilla DHIVA SYAFA QUAMILLA Efi Toding Tondok Elmi Muliya Elvina Efendi Fitrianingrum Kurniawati, Fitrianingrum GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Giyanto Giyanto GIYANTO GIYANTO GIYANTO GIYANTO Halimah Halimah Hamidi, Ilmi HARNI, RITA Heri Ahmadi Heriyanto Syafutra Hermanu Triwidodo Hishar Mirsam Ibrahim, Ahmad Yusuf IKA MUSTIKA IKA MUSTIKA Imamah, Annisa Nur Indahsari, Rizkhi Kikin H Mutaqin Lisnawita Lisnawita Lisnawita Lufthi Rusniarsyah, Lufthi Meity S. Sinaga MEITY S. SINAGA MEITY S. SINAGA Meity Suradji Sinaga Mohamad Rahmad Suhartanto Mokhammad Danang Kusuma Mubin, Nadzirum Muhamad Taher Muhammad Jabal Nur MUSTIKA, IKA Pradana Pandu Ankardiansyah R. YAYI MUNARA KUSUMAH RAUF, AUNU RITA HARNI Rita Harni RITA HARNI RITA HARNI RITA HARNI RITA HARNI Salsabila, Unik Hanifah Samsudin . Sembiring, Ade Indra Maulana Sitanggang, Della SOBIKHIN SOBIKHIN SRI HENDRASTUTI HIDAYAT Supriadi Supriadi Supriadi Supriadi SUPRIADI SUPRIADI SUPRIADI SUPRIADI Syarifah Syarifah, Syarifah TRI ASMIRA DAMAYANTI Utami, Budi Sri Victoria Coo Lea Wawan Kurniawan Widodo Widodo Yundari, Yundari Zalzilatul Hikmia Hikmia