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All Journal ACTA VETERINARIA INDONESIANA Jurnal Manajemen dan Agribisnis Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Kesehatan Lingkungan indonesia BERITA BIOLOGI Jurnal Medik Veteriner JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan Current Biomedicine Journal of Livestock and Animal Health (JLAH)
Herwin Pisestyani
Department Of Veterinary Public Health, Department Of Animal Disease And Veterinary Public Health, Faculty Of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Studi Kasus: Jumlah Sel Somatik dan Komposisi Susu dari Sapi Perah Positif Mastitis Subklinis Milik PT. Great Giant Livestock Jannatun Delvia; Arifin B. Nugraha; Agik Suprayogi; Herwin Pisestyani
JURNAL KAJIAN VETERINER Vol 11 No 1 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i1.8266

Abstract

An increase in the number of somatic cells to more than 400.000 cells/mL and the presence of pathogenic bacteria in the milk during normal lactation are two characteristics of subclinical mastitis. The purpose of this study was to compare subclinical mastitis-positive cows with healthy cows in terms of somatic cells count and milk composition, as well as the relationship between these two variables. Acquire from PT. Great Giant Livestock, a total of 29 fresh milk samples from 9 dairy cows tested positive for subclinical mastitis. The Breed method was used to determine the somatic cell count. Lactoscan was used to count the milk composition including fat, protein, lactose, solid non-fat, total solids, and density. The correlation between the somatic cell count and milk quality was tested using the Rank Spearman and the differences in milk quality between healthy cows and subclinical mastitis-positive cows were analyzed using Mann Whitney U. According to the data, subclinical mastitis-positive cows had more somatic cells than healthy cows. Furthermore, subclinical mastitis-affected cows provide milk of lower quality. Dairy cows with subclinical mastitis and healthy cows had significantly different milk composition (p< 0,05). The correlation between the number of somatic cells and milk composition was highly significant (p< 0,05). Subclinical mastitis-positive cows have a higher number of somatic cell and lower milk composition compared to healthy cows. The somatic cells count has a relationship with the composition of milk.
Keberadaan Escherichia coli Extended Spectrum ?-lactamase Resistan Antibiotik di Peternakan Sapi Perah Cijeruk, Bogor Herwin Pisestyani; Denny Widaya Lukman; Hadri Latif; Mirnawati Sudarwanto
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.505

Abstract

Contamination of Extended spectrum-betha lactamase (ESBL) producing Escherichia coli in milk can cause public health problems. The misuse of antibiotics in dairy has implications for the emergence of multi-resistant bacteria that can infect humans through food (foodborne diseases). The ESBL producing E. coli is resistant to ?-lactam antibiotics (including penicillins and 3rd and 4th derivatives of cephalosporins). This study aimed to analyze the phenotype of antibioticresistant ESBL-producing E. coli from livestock, farmer, dairy cows, milk, and pasteurized milk. The research was conducted on a dairy farm in Cijeruk District, Bogor. The method used was the ESBL EC tricycle for phenotype confirmation consisting of culture on tryptone bile x-glucuronide (TBX) agar and MacConkey agar (MCA) with the addition of the cefotaxime antibiotic 4 µg/mL for the presumption of ESBL-producing E. coli followed by confirmation of ESBL producing E. coli with indol biochemical tests with sulfite indole motility (SIM) and double disk diffusion test (DDST) on Mueller Hinton agar (MHA). The results from this study showed that ESBL producing E. coli was not found in water, effluent, milker hand swabs, teat swabs, and milk from the individual cows. ESBL producing E. coli was identified from fresh milk (2/10) and pasteurized milk (2/10) on the same farm. ESBL-producing E. coli were resistant to eight antibiotics (ampicillin, cefotaxime, ceftazidime, cefpodoxime, cephalothin, streptomycin, azithromicyn, enrofloxacin). Multidrugresistant ESBL-producing E. coli is one of the foodborne diseases that pose a health threat to the community.
Escherichia coli CONTAMINATION AND ITS RESISTANCE TO ANTIBIOTICS IN SE’I MEAT Gabryella Fransina Amalo; Trioso Purnawarman; Herwin Pisestyani
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18204

Abstract

The purpose of this study was to obtain Escherichia coli isolates from 11 se’i meat sellers in Kupang City and to observe their resistance to13 types of antibiotics. Escherichia coli were isolated and identified based on SNI 2897: 2008. Antibiotic resistance of the Escherichia colii isolates was determined using the Kirby-Bauer method. The results showed that 13 isolates (39.39%) of Escherichia coli in se’i meat samples had a high level of resistance to erythromycin (100%), tetracycline (76.92%), and doxycycline (61.54%). Isolates demonstrated increased resistance to streptomycin (46.15%), cephalothin (38.46%), trimethoprim-sulfamethoxazole (38.46%), amoxicillin (30.77%), chloramphenicol (30.77%), and choline sulfate (30.77%). The antibiotics nalidixic acid and ciprofloxacin demonstrated low Escherichia coli resistance (7.69%). Escherichia coli are sensitive to cefotaxime and gentamicin. A total of 12 isolates (92.31%) experienced MDR. The presence of non-MDR and MDR resistant Escherichia coli in se’i meat can seriously threaten community health.
Pengetahuan dan Preferensi Konsumen Yogurt di Kota Jambi Mayang Rosana; Adi Winarto; Herwin Pisestyani
Journal of Livestock and Animal Health Vol. 6 No. 1 (2023): February
Publisher : Politeknik Pertanian Negeri Payakumbuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32530/jlah.v6i1.7

Abstract

Yogurt adalah produk olahan susu yang diproses dengan cara fermentasi dan termasuk pangan fungsional, karena memiliki manfaat kesehatan yang besar bagi masyarakat. Tujuan penelitian ini adalah mengidentifikasi faktor-faktor yang memengaruhi pengetahuan dan preferensi konsumen Yogurt di Kota Jambi. Data kuesioner dikumpulkan dari 100 responden yang disebarkan melalui google form secara online. Karakteristik konsumen yogurt di Kota Jambi, yaitu sebagian besar perempuan (70%), berumur kurang dari 20 tahun (53%), berpendidikan menengah ke atas (SMA) (64%) dan berstatus sebagai pelajar atau mahasiswa (84%) yang diberikan uang jajan oleh orang tua kecil dari Rp 1.000.000,- per bulan (52%). Setiap bulannya, konsumen mengonsumsi yogurt secara rutin (58,75%) yang dibeli dari supermarket/minimarket (96,25%). Konsumen lebih menyukai yogurt cair (81,25%) dan dikemas botol plastik (60%), dengan rasa buah (83,75%), serta dikonsumsi langsung (78,75%). Bagi konsumen, yogurt adalah camilan (77,5%) yang disimpan di kulkas (±4 ºC) (88,75%). Tingkat Pengetahuan konsumen terhadap yogurt kurang baik (40,6%), sehingga pertimbangan konsumen dalam membeli yogurt yaitu logo halal 37%, masa kadaluarsa 35% , kebersihan tempat berjualan 25% dan terdapatnya logo NKV dalam kemasan sebanyak 3%.
Slaughterhouse’s Animal Welfare Assessement at Bekasi City, West Java Katyusa Nandini Dyah Pitaloka; Etih Sudarnika; Herwin Pisestyani
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.84696

Abstract

The quality of meat sold in Bekasi City is affected by the implementation of animal welfare during the slaughtering process in Slaughterhouses. The research aimed to analyze the animal welfare implementation during the slaughtering process at Bekasi City slaughterhouses. The data was conducted by observing the five-freedom implementation for five slaughterhouses throughout Bekasi City, from the shelter cages to the slaughter. Herding officers ensured no disturbance in the herding route, hitting only animals' backs when the animals stopped walking, and confirmed that animals did not pile up on the herding lane and that live animals did not see the slaughter process. However, some officers still wear clothing that contrasts with the environment, hit the animals repeatedly, and shout at the animals excessively during the herding process. Animal welfare aspects of the slaughter process, such as officers already in position when the animal enters the restraining box, the sharpness of the knife being checked before slaughtering, having a spare knife, and animals slaughtered less than 30 seconds after stunning, have been implemented by all officers. The butcher animal welfare implementation, such as checking the animals' death after being slaughtered, still needs to be improved. This research concluded that Bekasi City's slaughterhouse officers had implemented aspects of animal welfare. However, some improvements are still required through training to increase the competence of officers.
Kualitas mikrob dan sel somatik dalam susu yang berasal dari peternakan sapi perah di lokasi dataran tinggi dan dataran rendah Herwin; Jannah, Zahratul; Isdoni, Isdoni; Komala, Iyep; Pisestyani, Herwin
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.1-9

Abstract

Background: Consumers have the right to consume safe and healthy fresh milk. One of the factors that affect the productivity and quality of milk is the altitude of the location and environmental conditions around the farm. Objective: This research aimed to determine the differences in microbial quality and the somatic cell count (SCC) in milk between farms in the highlands (Cijeruk Farm, Bogor) and lowlands (Pondok Ranggon Farm, East Jakarta). Methods: This study observed the total plate count (TPC), the number of Staphylococcus aureus, and SCC. Microbial testing was carried out using the plate count method and the somatic cells count used the Breed method. The data were analyzed statistically using Mann-Whitney U and Chi-square. Results: The results showed that TPC, S. aureus, and SCC of milk from Pondok Ranggon located in the lowlands were higher than those of milk from Cijeruk located in the highlands. The average of TPC, S. aureus, and SCC in milk from Cijeruk were 1.6×104 ± 1.5×104 CFU/mL, 1.3×103 ± 4.2×103 CFU/mL, and 1,795,000 ± 1,838,791 cells/mL, respectively. The average of TPC, S. aureus, and SCC in milk from the Pondok Ranggon were 3.5×105 ± 1.0×106 CFU/mL, 1.7×105 ± 6.3×104 CFU/mL and 3,032,222 ± 4,348,654 cells/mL, respectively. This research showed that the location of the rearing area has relationship with S. aureus in milk (P<0.05). Conclusion: It was concluded that microbial status and the somatic cell count are influenced by differences in livestock areas.
Sanitation and Hygienic Practices of Ready-to-Drink Milk Seller Based on Total of Coliform and Staphylococcus aureus Pisestyani, Herwin; Ramadhani, Nada Nursaffana; Sudarwanto, Mirnawati; Lukman, Denny Widaya; Wicaksono, Ardilasunu
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.14-22

Abstract

Dairy products are innovative to improve the economic value of milk. Poor attention to the importance of good hygiene and milk handling of milk can lead to microbial contamination of milk, such as coliform and Staphylococcus aureus. This study aimed were to define the safety of ready-to-drink milk which sold around Dramaga and Cilibende IPB University campuses based on the total coliform and S. aureus and to determine the factors that affected it. Data were obtained by questionnaire from 12 sellers, furthermore milk samples were taken from 13 ready-to-drink milk's sellers with 3 replication The data were presented descriptively and analyzed using chi-square test to determine the relationship between the practice of the sellers with total contamination of coliform and S. aureus. The data results were compared with SNI 01-7388-2009 concerning the Maximum Limits of Microbial Contamination in Food. The average of coliform contamination in Dramaga and Cilibende 5,92í—103 ± 9,28í—103 CFU/ml and 7,60í—104 ± 1,25í—105 CFU/ml, respectively. The average of S. aureus contamination in Dramaga and Cilibende 3,84í—102 CFU/ml ± 5,36í—102 and 8,44í—104 ± 1,96í—105 CFU/ml, respectively. Furthermore, several practices of ready-to-drink milk sellers had a significant relationship with coliform contamination (p<0.05), which were the table cleanliness (p=0.020), distance to the crowd (p=0.001), and hand washing (p=0.001). The practice of ready-to-drink milk sellers had a significant relationship with S. aureus contamination (p<0.05) which were the table cleanliness (p=0.020), and distance of the crowd (p=0.020). The high contamination by coliform and S. aureus is caused by the poor sanitation and personal hygiene of the milk seller during the serving and processing of the milk.
An Evaluation of Draminski Detector as an Early Detection Tool for Subclinical Mastitis in Dairy Cattle in Pondok Ranggon Farm Pisestyani, Herwin; Permana, Indra; Basri, Chaerul; Lukman, Denny Widaya; Sudarwanto, Mirnawati
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.6-14

Abstract

Subclinical mastitis is one of the main problems in the development of dairy farming in Indonesia. Several screening test tools have been developed to support of control program for this problem. This study aimed to assess the suitability of the Draminski detector as a screening tool for subclinical mastitis in dairy cows. Quaternary milk samples from 27 dairy cows during normal lactation in the Pondok Ranggon area, East Jakarta were collected to be evaluated for the possibility of coming from quarters suffering from subclinical mastitis. Each milk sample was tested with 3 types of tests, i.e. the Breed method (golden standard) which was based on the number of somatic cells, the device Milkchecker which was based on electrical conductivity, and the Draminski detector which was based on electrical resistance. The results showed that the Draminski detector had a poor level of compatibility with the Breed method as the golden standard (Kappa value 0,013) and the Milkchecker tool (Kappa value 0,036). This test method was also considered to have poor test performance in diagnosing cases of subclinical mastitis in dairy cattle with a sensitivity value of 3,5%, specificity 100%, positive predictive value 100%, negative predictive value 18,8%, estimated prevalence 2,9%, and the true prevalence was 81,7%. In conclusion, the Draminski detector cannot be used to replace the Breed method and Milkchchecker as a screening test tool in cases of subclinical mastitis in dairy farms in Indonesia which were generally reared in the dairy farms.
Studi Kasus: Pemalsuan Daging Sapi dengan Daging Babi Hutan di Kota Bogor Nida, Lailatun; Pisestyani, Herwin; Basri, Chaerul
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.2326

Abstract

The adulteration of beef using wild boar meat in the city of Bogor has been a serious concern in the society. Monitoring and surveillance of beef products are needed to ensure the halal of animal products and to prevent the transmission risk of zoonotic diseases from wild boar meat to humans. The purpose of this study was to analyse the data of Dinas Pertanian Kota Bogor related to meat adulteration in 2013-2017. The case study approach was used in this research by conducting a collection of primary and secondary data. The primary data obtained from an indepth interview with the chief of veterinary public health, processing, and marketing of livestock products of Dinas Pertanian Kota Bogor. The secondary data were obtained from monitoring and surveillance report of Dinas Pertanian Kota Bogor in 2013-2017. The results showed that 7.86% (3/33 samples) of beef samples contained wild boar meat during the 2013-2017 period. The adulterated beef was found mainly from the meat kiosks in traditional markets. In conclusion, monitoring and surveillance related to meat adulteration problem is needed to be improved especially in the traditional market.
Kejadian Bruselosis Pada Sapi Potong dan Pemetaan Wilayah Berisiko di Kabupaten Barru Provinsi Sulawesi Selatan Tahun 2015-2017 Fitria, Nisa Nurul; Pisestyani, Herwin; Wicaksono, Ardilasunu
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.2843

Abstract

There is still lack of bruselosis in beef cattle in Barru District, South Sulawesi. The aim of this study was to analyze data about the temporary distribution of disease by measuring spreading speed, and spatial distribution by mapping risk areas for bruselosis over the past three years. The data of this study was collected using the records from Dinas Peternakan and conducting interviews using structured questionnaires. This research was a descriptive study by measuring the incidence rate and describing the risk map using geographic information system (GIS). The results of this study indicate that, based on the incidence rate, the average of distribution rate of bruselosis in beef cattle in Barru is 5 cases per 10 000 heads/year. This incidence rate always decreases every year. There was no sub-district that classified as high risk. There was one area that classified as medium risk namely sub-district of Mallusetasi. Control measure that have been carried out by goverment were successful to reduce the spread of disease.
Co-Authors Abdul Zahid Adi Winarto Agik Suprayogi Ahmad, Hasniah Aminudin, Muhammad Rizki Andriani Andriani Anggraini, Raden Isma annisa, rizka fajri Ardilasunu Wicaksono Arifin B. Nugraha Bambang Pontjo Priosoeryanto Chaerul Basri Denny Lukman Denny Widaya Lukman Devi Yanti Sari Etih Sudarnika Fitria, Nisa Nurul Gabryella Fransina Amalo Hadri Latif Harsi D. Kusumaningrum Herwin Iccha Pradipta Patsiwi Indra Permana, Indra Irena Ivania Isdoni Bustaman Jannah, Zahratul Jannatun Delvia Katyusa Nandini Dyah Pitaloka koekoeh santoso Komala, Iyep Lymbran Tina Maya Shofa Mayang Rosana Mila Meha, Margie Pristiantine Milawarni Milawarni Mirnawati B. Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Muhammad Pauzi Lubis Nadhear Nadadyanha Dannar Naoaki Misawa Nida, Lailatun Nugraha, Arifin Budiman Ramadhani, Nada Nursaffana Shodiq, Nadim Sudarwanto, Mirnawati Bachrum Supriyono, Supri Surachmi Setiyaningsih Surachmi Setyaninigsih Susanti, Oli Tangkonda, Elisabet Trioso Purnawarman Wahyudi, Ading Yunindika, Thufeil Yusuf Ridwan