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Herwin Pisestyani
Department Of Veterinary Public Health, Department Of Animal Disease And Veterinary Public Health, Faculty Of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Manpower Planning in The Application of Teat Dipping To Control Sub Clinical Mastitis in The Small Dairy Farms Anggraini, Raden Isma; Sudarnika, Etih; Pisestyani, Herwin; Wicaksono, Ardilasunu; Sudarwanto, Mirnawati
Jurnal Manajemen & Agribisnis Vol. 17 No. 1 (2020): JMA Vol. 17 No. 1, March 2020
Publisher : School of Business, Bogor Agricultural University (SB-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.042 KB) | DOI: 10.17358/jma.17.1.26

Abstract

The application of teat dipping to control subclinical mastitis especially in small dairy farms is important; however, the application needs additional activity that has an impact on labor usage. This paper aimed to describe the labor usage to apply teat dipping for subclinical mastitis control in the small dairy farms. The convenience sampling techniques applied to select 34 farmers in Bogor as the respondents. Workload analysis and labor productivity were used in examining the application of dot dipping in various small dairy farms in Bogor City to control subclinical mastitis. This study was based on two types of experiments in small dairy farms in Bogor, West Java, namely teat dipping and without teat dipping. The findings show that the application of teat dipping had an impact on three things, namely an increase in labor usage and its workload, an increase in labor productivity and a decrease in the number of subclinical mastitis prevalence. This study concluded that labor was an important factor to control subclinical mastitis in small dairy farms. An increase in labor productivity had an impact on reducing the costs of mastitis treatment and finally increase farmer’s income. Keywords: mastitis, labor, small dairy farms, teat dipping, workload analysis
Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Multidrug-Resistant (MDR) E. coli dalam Daging Babi yang Berasal dari Rumah Potong Hewan (RPH) Oeba, Kota Kupang Mila Meha, Margie Pristiantine; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Acta VETERINARIA Indonesiana Vol. 12 No. 2 (2024): Juli 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.2.168-175

Abstract

To produce pork ready for consumption, it must go through various stages, from the farm to the packaging. The chances of contamination with pathogenic microbes are very large. Pathogenic microbes are often found in pork, one of which is E. coli, which is often the trigger for foodborne disease in humans. Previous studies have shown that E. coli isolates from pig farms in Kupang City are known to have multidrug resistance (MDR), which, if this event spreads, could negatively impact the community. RPH is a place for slaughtering animals supervised by veterinarians to produce good quality meat. This study aims to measure E. coli contamination, identify and isolate MDR E. coli from fresh pork from Oeba RPH, Kupang City. The enumeration number of E. coli was carried out based on the Indonesian National Standard (SNI) ISO 16649–2:2001 concerning the Horizontal Enumeration Method for E. coli positive-b-glukuronidase - Part 2: Colony counting technique at 44 °C using 5-bromo-4-chloro-3-indolyl-b-D-glucuronide. Resistance test of E. coli to antibiotics was carried out based on the Kirby-Bauer method. Data related to the amount of E. coli contamination and testing of E.coli antibiotic resistance were analyzed descriptively. The results showed that the contamination of E. coli in the pork used had an average of 626,440 ± 947,937 CFU/g. All E. coli isolates were resistant to penicillin (100%), most E. coli isolates were resistant to streptomycin (80%) and tetracycline (60%), and half of the E. coli isolates were resistant to oxytetracycline (47%) and amoxicillin (40%). E. coli isolates obtained from pork had MDR against 2 (7/15;46.67%) to 3 (7/15;46.67%) groups of antibiotics. This study concludes that the contamination of E. coli in pork from Oeba slaughterhouse exceeds the maximum microbial contamination limit (>1x101 CFU/g). E. coli isolates were resistant to penicillin, streptomycin, tetracycline, oxytetracycline, and amoxicillin and had MDR against 2 to 3 classes of antibiotics.
Keanekaragaman Ektoparasit di Peternakan Sapi Potong Astomulyo Lampung Tengah dan Peternakan Sapi Perah Kawasan Usaha Peternakan Rakyat (KUNAK) Bogor Shodiq, Nadim; Supriyono, Supriyono; Pisestyani, Herwin
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20699

Abstract

Ectoparasites in livestock areas can cause losses to farmers, such as decreased body weight and milk production due to anemia, disease transmission, and animal discomfort. This study aimed to identify the diversity, abundance, and potential risk of diseases that can be transmitted by ectoparasites as disease vectors in cattle. The study was conducted in two selected locations: Astomulyo beef cattle farm in Central Lampung and KUNAK dairy farm in Bogor. All ectoparasites around the pens were collected using sweep nets, aspirators, light traps and forceps. Identification results showed a diverse range of ectoparasites, including mosquitoes (Culicidae), flies (Muscidae), ticks (Haemaphysalis), Culicoides (Ceratopogonidae) and fleas (Haematopinidae). The environment, such as pens conditions, storage facilities, manure management, feed waste, and sanitation, influenced the diversity of ectoparasite species found in the study sites. The identified ectoparasite species have the potential to be vectors of various livestock diseases that lead to decreased production. This study is expected to provide information related to effective disease prevention and control strategies.
Safety Risk Factors of Duck Meat Sold in Bogor City annisa, rizka fajri; Purnawarman, Trioso; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.101423

Abstract

With the increasing demand for duck meat in Bogor City, this poses a risk of the presence of pathogenic bacteria throughout the food supply chain. Traditional markets are one of the places that have a high risk of contamination with pathogenic bacteria that can endanger public health. This study aims to determine the presence of Salmonella and E. coli in duck meat sold in traditional markets in Bogor City and the risk factors that influence the presence of these pathogenic bacteria. This study was a cross sectional study with a total of 45 duck meat samples obtained from 3 traditional markets in Bogor City. Samples were taken to the SKHB IPB Veterinary Public Health Laboratory for microbiological testing. Risk factors that may affect the safety of duck meat were assessed using a questionnaire. The results showed that 13 samples (29%) were positive for Salmonella and 27 samples (60%) were positive for E. coli. The presence of Salmonella and E. coli in duck meat indicates cross-contamination during slaughter, evisceration and poor sanitation and hygiene practices. Continuous monitoring and reporting of the presence of Salmonella and E. coli is needed so that duck meat consumed by the public is safe for consumption.
Penerapan Sanitasi dan Higiene dalam Produksi Ikan Asap: Implikasi terhadap Keamanan Pangan dan Kesehatan Masyarakat Tina, Lymbran; Sudarnika, Etih; Ridwan, Yusuf; Sudarwanto, Mirnawati B.; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.105214

Abstract

Keamanan pangan merupakan aspek krusial dalam kesehatan masyarakat, terutama pada produk pangan yang diproses secara tradisional seperti ikan asap. Penelitian ini bertujuan mengevaluasi penerapan sanitasi dan higiene dalam produksi ikan asap di Kota Kendari serta mengkaji dampaknya terhadap keamanan pangan dan kesehatan masyarakat. Penelitian menggunakan desain observasional deskriptif dengan pengambilan sampel secara purposive pada 13 unit produksi ikan asap selama Juli sampai Oktober 2024. Data dikumpulkan melalui observasi langsung dan checklist kepatuhan terhadap standar Badan Pengawas Obat dan Makanan (BPOM). Hasil penelitian menunjukkan kurang dari sebagian produsen (48,4%) yang memastikan peralatan produksi tetap bersih dan terawat secara menyeluruh. Seluruh produsen (100%) menggunakan air bersih selama produksi, namun tanpa fasilitas pembersihan bahan baku yang memadai. Toilet yang memenuhi standar kebersihan hanya ditemukan pada 69,2% lokasi produksi, sementara tidak ada satu pun tempat sampah tertutup (0%). Evaluasi praktik higiene pekerja, didaptkan sebanyak 76,9% pekerja mencuci tangan sebelum dan sesudah bekerja, namun tidak ada pekerja yang mamakai alat pelindung diri (0%). Selain itu, seluruh lokasi produksi tidak memiliki program pembersihan dan sanitasi berkala (0%), dan terdapat hewan peliharaan di sekitar area produksi, yang dapat meningkatkan risiko kontaminasi mikroorganisme patogen. Kondisi ini menunjukkan bahwa industri ikan asap tradisional di Kendari belum memenuhi stadar sanitasi dan higiene yang baik sehingga memerlukan intervensi berbasis kesehatan masyarakat. Diperlukan edukasi kepada produsen mengenai pentingnya sanitasi dan higiene, peningkatan infrastruktur sanitasi, serta pengawasan lebih ketat dari pihak berwenang guna memastikan kepatuhan terhadap standar keamanan pangan. Kata kunci: keamanan pangan, higiene, kesehatan masyarakat, sanitasi, ikan asap
Co-Authors Abdul Zahid Adi Winarto Agik Suprayogi Ahmad, Hasniah Aminudin, Muhammad Rizki Andriani Andriani Anggraini, Raden Isma annisa, rizka fajri Ardilasunu Wicaksono Arifin B. Nugraha Bambang Pontjo Priosoeryanto Chaerul Basri Denny Lukman Denny Widaya Lukman Devi Yanti Sari Etih Sudarnika Fitria, Nisa Nurul Gabryella Fransina Amalo Hadri Latif Harsi D. Kusumaningrum Herwin Iccha Pradipta Patsiwi Indra Permana, Indra Irena Ivania Isdoni Bustaman Jannah, Zahratul Jannatun Delvia Katyusa Nandini Dyah Pitaloka koekoeh santoso Komala, Iyep Lymbran Tina Maya Shofa Mayang Rosana Mila Meha, Margie Pristiantine Milawarni Milawarni Mirnawati B. Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Muhammad Pauzi Lubis Nadhear Nadadyanha Dannar Naoaki Misawa Nida, Lailatun Nugraha, Arifin Budiman Ramadhani, Nada Nursaffana Shodiq, Nadim Sudarwanto, Mirnawati Bachrum Supriyono, Supri Surachmi Setiyaningsih Surachmi Setyaninigsih Susanti, Oli Tangkonda, Elisabet Trioso Purnawarman Wahyudi, Ading Yunindika, Thufeil Yusuf Ridwan