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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Penelitian Sains (JPS) Matematika dan Sains Medical Journal of Indonesia Jurnal Penelitian Sains (JPS) IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) BERITA BIOLOGI JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA JPSriwijaya Science and Technology Indonesia Jurnal Penelitian Sains Indonesian Journal of Chemistry Indonesian Journal of Pure and Applied Chemistry IJEMS (Indonesian Journal of Environmental Management and Sustainability) Martabe : Jurnal Pengabdian Kepada Masyarakat Seminar Nasional Lahan Suboptimal Molekul: Jurnal Ilmiah Kimia BIOVALENTIA: Biological Research Journal Jurnal Ilmiah Bakti Farmasi SWARNA Jurnal Riset Rumpun Ilmu Kesehatan (JURRIKES) Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia Journal of World Future Medicine, Health and Nursing SWARNA: Jurnal Pengabdian Kepada Masyarakat Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian Kepada Masyarakat
Miksusanti Miksusanti
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Sriwijaya University, Palembang, Indonesia
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Journal : BERITA BIOLOGI

 1 
KERUSAKAN DINDING SEL Escherichia coli OLEH MINYAK ATSIRI TEMU KUNCI (Kaempferiapandurata) Miksusanti, Miksusanti; Jennie, Betty Sri Laksmi; Ponco, Bambang; Trimulyadi, Gatot
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.201 KB) | DOI: 10.14203/beritabiologi.v9i1.775

Abstract

Antibacterial activity of temu kunci (Kaempferiapandurata) essential oil against Escherichia coli Kl.l was analyzed. Activity of antibacterial essential oil was analyzed through its ability to leak the Escherichia coli Kl.l cell wall and altering it.Minimum Inhibitory Concentration (MIC) of temu kunci essential oil is 0.11% (v/v). Further studies were conducted using the concentration of 1 MIC and 2 MIC.Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry(UV).Alteration of cell wall was analyzed with scanning electron microscopy (SEM).The optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm.The result showed that K. pandurata essential oil could leak the inorganic ion Ca 17-53%, and K* 9-43% from the bacteria and alter the cell wall of the bacteria.
KERUSAKAN DINDING SEL Escherichia coli OLEH MINYAK ATSIRI TEMU KUNCI (Kaempferiapandurata) Miksusanti Miksusanti; Betty Sri Laksmi Jennie; Bambang Ponco; Gatot Trimulyadi
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.775

Abstract

Antibacterial activity of temu kunci (Kaempferiapandurata) essential oil against Escherichia coli Kl.l was analyzed. Activity of antibacterial essential oil was analyzed through it's ability to leak the Escherichia coli Kl.l cell wall and altering it.Minimum Inhibitory Concentration (MIC) of temu kunci essential oil is 0.11% (v/v). Further studies were conducted using the concentration of 1 MIC and 2 MIC.Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry(UV).Alteration of cell wall was analyzed with scanning electron microscopy (SEM).The optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm.The result showed that K. pandurata essential oil could leak the inorganic ion Ca 17-53%, and K* 9-43% from the bacteria and alter the cell wall of the bacteria.
Co-Authors Adi Supriyadi Adik Ahmadi Agus Syafriansyah Al Ainna Aldes Lesbani Almunadi T Panagan Almunady T. Panagan Andarmoyo, Sulistyo Annisa Amelia Asmak Asmak Azhar Kholiq Azhar Kholiq Bambang Ponco Bambang Ponco Bambang Ponco, Bambang Bambang Pontjo Priosoeryanto Betty S.L. J Betty Sri Laksmi J Betty Sri Laksmi Jenie Betty Sri Laksmi Jennie Budi Untari Budi Untari Darsil Basir Dasril Basir Dasril Basir Dasril Basir Dedi Rohendi Desnelli Desnelli Desnelli Desnelli Dian Dian Dian Dwita Maizur Dimas Nur Hidayat Elfita Eli Sahara Eli Sahara Elsa Fitria Apriani Endang Setiyowati Endang Setiyowati Endri Junaidi Eviyanti Eviyanti Fachrijal Fachrijal Fanani, Muhammad Zainal Fatma Fitra Yosi Fitrya . Fitrya Fitrya Gatot T. Mulyadi Gatot Tri Mulyadi Gatot Trimulyadi Gatot Trimulyadi Rekso Getari Kasmiarti Harian Saputra Harmida Harmida Heni Yohandini Heni Yohandini K Herlina Herlina Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Hotdelina S Hotdelina S Ina Suci Pratiwi Indah Solihah Indah Solihah Ismed Ismed Jefri Liasta Jennie, Betty Sri Laksmi Juswardi Juswardi Maidelwita, Yani Meisji Liana Sari Muharni Muharni Muntasir, Muntasir Nasir Rofiq Neza Rahayu Nike Marfinda Nirwan Syarif Nita Aminasih Nova Yuliasari Novian Sutami Novrikasari Novrikasari, Novrikasari NUNI GOFAR Rennie Puspa Novita Risfidian Mohadi Rizal Syarief Rizal Syarief Rizal Syarief Setiawan Yusuf Setiawan Yusuf Setiawaty Yusuf Sofia Sandi Sukmawati Sukmawati Susilawati Susilawati Syaflina Lamin Syafrina Lamin, Syafrina Syndi N.K Tridayona Tridayona Trimulyadi, Gatot Wiwin Wiwin Yusup Nur Khakim, Mokhamad