TRI ASMIRA DAMAYANTI
Departemen Proteksi Tanaman, Fakultas Pertanian IPB, Jln. Kamper, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia, 16680

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Management effort of mottle virus by leaf extract of moringa (Moringa oleifera Lam.) and bougainvillea (Bougainvillea spectabilis Willd.) on peanut Zulfi, Fahma Zakiya; Damayanti, Tri Asmira; Giyanto, Giyanto
Agrovigor Vol 17, No 1 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i1.25086

Abstract

Peanut mottle virus is one of peanut production constrain worldwide. It is transmitted by aphid vectors and by seeds. One of the efforts to manage viruses is by using plant extracts. This study aims to test the potency of moringa and bougainvillea leaf extract to suppress mottle virus infection in peanuts. The test consisted of four treatments: healthy control, untreated control infected by the virus, moringa, and bougainvillea leaf extracts treatment infected by the virus. The disease assessment and agronomic parameters were observed weekly. The results showed that the spray application of moringa and bougainvillea leaf extract showed lower disease incidence, significantly able to prolong the incubation period, decreased disease severity, and as well as virus titer significantly in comparison to the control. The area under the disease progress curve among leaf extract treatments was significantly different but was significantly lower in comparison to untreated control infected by the virus. The germination percentage, vigor index, and plant height were not significantly different among treated plants and control, except for the leaf numbers. In addition, the growth vigor of treated plants was better than the control. These indicate that both plant leaf extracts have antiviral activity and the ability to improve plant growth.
The effect of chitosan on enhancing the defense mechanisms of yard long bean plants against Aphis craccivora Koch. Megasari, Dita; Santoso, Sugeng; Damayanti, Tri Asmira
Agrovigor Vol 18, No 1 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i1.29644

Abstract

Yard-long bean production in Indonesia has declined due to environmental factors and pest infestations, particularly by Aphis craccivora, a vector of viruses such as Bean common mosaic virus (BCMV). Generally, A. craccivora has been controlled using insecticides; however, their use poses limitations including toxicity, the development of resistance, environmental concerns, and disruption of pest–predator dynamics due to excessive application. As a promising alternative, chitosan has demonstrated potential in inhibiting aphid feeding, reducing reproduction rates, delaying disease incubation periods, and lowering BCMV titres. This study assessed the effects of both pure and commercial chitosan on A. craccivora in yard-long beans. The evaluation focused on antixenosis, antibiosis, and insecticidal properties. The results showed that chitosan significantly reduced aphid colonisation and feeding preference, lowered infestation intensity, and enhanced natural predation. Furthermore, chitosan treatments suppressed aphid reproduction, prolonged the aphid life cycle, and decreased their growth rate. The direct spray method was found to be more effective than the systemic application. Among the treatments, KK 0.9 consistently produced the most favourable outcomes across all parameters, indicating its potential as an effective bio-insecticidal agent for pest and disease management in yard-long bean cultivation.
Diseases in tobacco with monoculture and polyculture farming systems Januarsih, Vera; Khairani, Hagia Sophia; Damayanti, Tri Asmira
Agrovigor Vol 18, No 1 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i1.29407

Abstract

Tobacco (Nicotiana tabacum L.) is an important agricultural product in Indonesia. Disease information on tobacco is required to develop effective control methods for supporting a proper tobacco farming system. This study investigates the disease type and intensity of tobacco in monoculture and polyculture cropping systems in Patebon District, Kendal Regency, Central Java. Disease evaluation factors such as types of symptoms, disease incidence and severity, and crop productivity under both crop systems were measured on tobacco every week for four weeks from 14 to 17 weeks after planting, as well as during harvest. Microscopic observation was used to identify the causative infections. The findings revealed that the disease makeup differed amongst crop systems. The leaf curl (Tobacco leaf curl virus) and mosaic (Tobacco mosaic virus) diseases were discovered in both cropping systems. However, anthracnose (Colletotrichum sp.) and leaf spot (Cladosporium sp.) were only observed in monoculture farming systems, whereas wildfire (Pseudomonas syringae pv. tabaci) was only identified in polyculture farming systems at a low incidence. In general, disease incidence and severity were higher in monoculture agriculture systems than in polyculture crops. Furthermore, the policulture crop system had a slightly higher yield, indicating that tobacco would be better planted in polyculture than monoculture crop systems.
Antimicrobe activity of empty bunches of palm tree’s liquid smoke against Sclerotium rolfsii Sacc. and Puccinia arachidis Speg. 1884 Pratiwi, Rizky Nomi; Tondok, Efi Toding; Damayanti, Tri Asmira
Agrovigor Vol 18, No 1 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i1.29632

Abstract

Groundnut (Arachis hypogaea L.) is one of the food crops that has high economic value. However, one of the constraints to groundnut production is the stem base rot (Sclerotium rolfsii) and rust (Puccinia arachidis) diseases. Liquid smoke is known to have antimicrobial activities that need to be further studied for their potential to control various types of pathogens. This study aimed to test the ability of liquid smoke from empty palm bunches in controlling S. rolfsii and P. arachidis in vitro. The phytotoxicity of liquid smoke to seeds was tested using a rolled paper test established in plastic. Antimicrobial activity was tested by using the poisoning medium method against S. rolfsii, while spore germination test against P. arachidis was conducted by direct liquid smoke dripping onto uredospore on object glass. Antimicrobial activity of liquid smoke at concentrations of 0.1%, 0.5%, 1.0%, 1.2%, and 1.4% showed growth inhibition of S. rolfsii by 2.0%, 5.4%, 35.0%, 78.8%, and 100%, respectively. At 24 hours after treatment, the effect of liquid smoke on P. arachidis spore germination at concentrations of 0.1% and 1.0% was 1.7% and 0.0% when compared to the control, 8.4%, and at 48 hours after treatment was 1.5% and 0.0% compared to the control, 9.7%. It indicates the ability of liquid smoke as an antifungal. 
The Effectiveness of Integrated Management Packages in Controlling Aphid-Borne Virus Infections on Groundnut in South Sulawesi, Indonesia Kasim, Niken Nur; Damayanti, Tri Asmira; Hidayat, Sri Hendrastuti; Santoso, Sugeng
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4320

Abstract

Aphid-borne viral infection is a major limiting factor for groundnut production in South Sulawesi, Indonesia. The study aimed to elucidate the effectiveness of integrated management packages (IMP) in controlling aphid-borne viruses infecting groundnut in the field. Field trials were conducted in a plot without (M0) and with plastic mulch and maize as a plant barrier (M1), while two different groundnut varieties, Bougainvillea leaf extract, and insecticides were incorporated as the subplot treatments. The treatments in M1 plot significantly reduced the aphid population (17.4–79.9%) and AUDPC incidence (51.7–87.3%), and severity (61.0–92.9%), respectively. The treatments increased dry pod production by 11.9–63.3% depending on subplot treatment compared to treatments in the M0 plot (p>0.05). Bean common mosaic virus strain PSt and Cowpea Polerovirus 2 were positively detected in groundnuts as well as in aphids using RT-PCR. Further, nine weed species in the vicinity of the field trial were found to be positively infected with BCMV-PSt, indicating their crucial role as reservoir hosts. Interestingly, the effectiveness of Bougainvillea leaf extract treatment in the M1 plot was comparable to that of the insecticide treatment, indicating that this IMP is a more cost-effective, eco-friendly groundnut virus control than the currently used method.
Pengendalian Aphis craccivora Koch. dengan kitosan dan pengaruhnya terhadap penularan Bean common mosaic virus strain Black eye cowpea (BCMV-BlC) pada kacang panjang Megasari, Dita; Damayanti, Tri Asmira; Santoso, Sugeng
Jurnal Entomologi Indonesia Vol 11 No 2 (2014): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.2 KB) | DOI: 10.5994/jei.11.2.72

Abstract

Aphis craccivora is one of the important pests on yard long bean. It causes direct damage and also has an indirect effect as insect vector of the Bean common mosaic virus (BCMV). The research was done to test the effectiveness of chitosan in suppressing aphid population growth, feeding preference and its ability in transmiting BCMV. Chitosan with concentration ranging of 0.1–1.0% were applied on leaves using spraying method at 1 day before BCMV transmission. BCMV was transmitted by using 3 individuals of viruliferous aphids on each plant. Results show that chitosan treatments on leaves or plants significantly suppressed the population and feeding preferences of A. craccivora. Further, treated plants showed lower disease incidence, severity and BCMV titre significantly compared with untreated control plants. The positive effects of chitosan in suppressing population growth as feeding preferences and BCMV transmission might be due to the anti-feedant effect of chitosan on A. craccivora. Based on the result, chitosan at concentration 0.9% is the most effective concentration in suppressing BCMV and its vector A. craccivora.
Diseases in mung bean with the spreading and dibbling planting system in Banyumas Regency, Central Java, Indonesia Primandani, Dhika Kintania; Khairani, Hagia Sophia; Damayanti, Tri Asmira
Agrovigor Vol 18, No 2 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i2.31651

Abstract

Mung bean is Indonesia's important legume crop after soybean and peanut, and it can be grown as a primary crop or an intercrop. The purpose of this study was to compare disease development and yield on mung bean fields grown under a spreading and dibbling cropping system in Kedungwringin Village, Jatilawang District, Banyumas Regency, Central Java. Data was collected from farmers through interviews and weekly disease observation in six mung bean fields. The type of disease, disease incidence, and severity were also determined using data obtained directly from the field. The study discovered five fungi and virus: powdery mildew (Erysiphe sp.), stem rot (Sclerotium rolfsii), leaf rust (Uromyces sp.), leaf spot (Cercospora sp.), and yellow mosaic disease (Begomovirus). The incidence of such diseases was ranging from 1% up to 85%, with the yellow mosaic disease having a very low incidence. There is no significant difference of disease intensity on both system. Leaf spot was the major disease with severity increasing gradually from 11.11% to 43.17%. Several insect pests and predators were discovered in fields. Considering the higher pod production in response to the disease occurrence, the dibble cropping system is recommended as the better mung bean farming strategy.
Potensi Carbon dots Kulit Nanas dan Belimbing Wuluh untuk Mengendalikan Infeksi Tobacco mosaic virus dan Kutu Daun Huriyah, Zahrotul; Damayanti, Tri Asmira; Santoso, Sugeng; Maddu, Akhiruddin
Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.69467

Abstract

Tobacco mosaic virus (TMV) dan kutu daun merupakan organisme pengganggu tanaman (OPT) yang penting dalam bidang pertanian. TMV mudah ditularkan secara mekanis, virusnya sangat stabil, tular benih dan memiliki kisaran inang luas. Sementara itu, kutu daun merupakan hama kosmopolit dan sebagian besar berperan sebagai vektor virus. Saat ini tren pengelolaan OPT mengarah pada metode ramah lingkungan. Pemanfaatan Carbon dots (CDs), dalam pengelolaan OPT sebagai salah satu agens yang menjanjikan untuk pengelolaan OPT yang berkelanjutan, namun kajiannya masih terbatas. Penelitian ini bertujuan menyintesis CDs dari kulit nanas dan belimbing wuluh serta menguji potensinya dalam mengendalikan TMV dan kutu daun. CDs dikarakterisasi berdasarkan sifat optik, gugus fungsi, dan morfologi. Aktivitas antivirus diuji terhadap infeksi mekanis TMV pada tanaman indikator Chenopodium amaranticolor dengan penyemprotan daun sebelum dan sesudah inokulasi virus. Aktivitas insektisida CDs diuji terhadap Aphis gossypii melalui penyemprotan langsung pada kutu daun pada konsentrasi 100–400 ppm. Kedua jenis CDs berhasil disintesis dengan ukuran 5.5-9.8 nm dengan bentuk amorf dan kristal, serta memiliki karakter optik dan gugus fungsi yang khas sebagai CDs. Perlakuan CDs secara nyata memperpanjang periode inkubasi TMV dibandingkan kontrol. Aplikasi penyemprotan sebelum inokulasi virus lebih efektif dibandingkan setelah inokulasi, dengan tingkat penghambatan gejala lesio lokal nekrotik berkisar 31%–91% bergantung pada konsentrasi. Pada konsentrasi 400 ppm, CDs berpotensi sebagai bioinsektisida menyebabkan mortalitas kutu daun hingga 55%. Hal ini mengindikasikan bahwa CDs dari kedua bahan alami berpotensi sebagai penginduksi ketahanan tanaman, memiliki aktivitas antivirus, dan aktivitas insektisida.
Efektivitas Gelombang Ultrasonik dalam Eliminasi Nematoda Daun Aphelenchoides pada Umbi Bawang Merah Kurniawati, Fitrianingrum; Supramana, Supramana; Hidayat, Sri Hendrastuti; Tondok, Efi Toding; Syafutra, Heriyanto; Damayanti, Tri Asmira; Mubin, Nadzirum
Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68192

Abstract

Nematoda daun Aphelenchoides merupakan salah satu patogen tanaman yang memiliki kisaran inang yang luas dan menyebabkan kehilangan hasil panen. Metode konvensional telah sering digunakan dalam pengendalian nematoda termasuk nematisida kimia, perawatan air panas, dan nematisida hayati tetapi memiliki keterbatasan. Hal ini memerlukan eksplorasi metode alternatif yang ramah lingkungan dan efektif, seperti teknologi gelombang ultrasonik. Penelitian bertujuan mengetahui efektivitas gelombang ultrasonik dalam eliminasi nematoda daun Aphelenchoides pada umbi bawang serta pengaruh pada pertumbuhan umbi bawang. Ekstraksi nematoda menggunakan metode pengabutan (mist chamber) dan perendaman air dingin. Nematoda diidentifikasi berdasarkan karakter morfologi. Umbi bawang merah yang telah diinfestasikan Aphelenchoides diberi perlakuan ultrasonik dengan lama perlakuan selama 26, 30, 37, 44, 60 menit dan kontrol. Umbi bawang yang telah diberi perlakuan ultrasonik ditanam di pot dan diamati parameter pertumbuhannya sampai panen. Data ditabulasikan di Microsoft Excel dan diolah menggunakan SAS JMP. Perlakuan ultrasonik dengan durasi yang lebih dari 37 menit menghasilkan tingkat mortalitas yang tinggi (≥84,00%).  Waktu papar yang berbeda menunjukkan pengaruh yang tidak berbeda nyata dengan kontrol pada tinggi tanaman dan panjang akar. Lebih lanjut, perlakuan ultrasonik dengan durasi yang lebih lama (≥ 37 menit) sangat efektif dalam menekan populasi nematoda Aphelenchoides tanpa memengaruhi pertumbuhan tanaman bawang merah.
Identifikasi Spesies Aphelenchoides pada Benih Padi Varietas Lokal Sumatera Utara Berdasarkan Marka Molekuler Della Sitanggang; Supramana; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 22 No. 1 (2026): Maret 2026: IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.22.1.7-11

Abstract

The nematode Aphelenchoides besseyi was successfully detected and morphologically identified in the rice seeds of Samosir local varieties, namely ‘Siserang’, ‘Sipining’, ‘Sibandung’, and ‘Saratus Ari’. This study aimed to confirm the identity of Aphelenchoides species using universal primers as markers for the D2-D3 expansion region of the 28S rDNA gene. Nematodes were extracted from the seeds of the North Sumatra local varieties including ‘Siserang’, ‘Sipining’, ‘Sipala’, ‘Sirias’, ‘Sigambiri Merah’, and ‘Sigambiri Putih’. Nematodes from the commercial rice variety ‘Pak Tiwi-1’ was used for comparison. DNA extraction of A. besseyi nematodes was carried out using the Holterman method. The amplification process used universal primers D2A-D3B, producing a DNA fragment of approximately 750 bp. DNA amplification of A. besseyi from the Samosir, Simalungun, and Pakpak Bharat Districts successfully produced DNA fragments of approximately 750 bp. Nematodes obtained from the Siserang, Sigambiri Merah, Sirias, Sigambiri Putih, Sipala, and Sipining varieties showed 99.8%–100% homology with A. besseyi isolates from the Indonesia commercial rice variety, Japan, Brazil, and China.
Co-Authors . Giyanto . Haryanto Abdjad Asih Nawangsih Ade Syahputra Akhiruddin Akhiruddin Akhiruddin Maddu Anastasya Hondo Anastasya Hondo Annisa Nur Imamah Cahyati, Iwe Della Sitanggang Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dita Megasari Dwi Subekti Efendi, Darda Efi Toding Tondok Endah Muliarti Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusti Ngurah Alit Susanta Wirya Haerani Haerani Hagia Sophia Khairani Hamdayanty Hamdayanty HENDRA PARDEDE Heriyanto Syafutra Huriyah, Zahrotul I Dewa Nyoman Nyana Ida Parida Imamah, Annisa Nur Imaniasita, Vidya IRWAN LAKANI Islami, Nisa Fadhila Januarsih, Vera Jati Adiputra Kartika Catur Damaiyanti Ketut Ayu Yuliadhi Kikin H Mutaqin L Pebriyeni Ladja, Fausiah T. Laras Anjarsari Lilik Koesmihartono Putra Listihani, Listihani Lulu Kurnianingsih Martha Theresia Panjaitan Melinda . Mhd Rifqi Abdillah Miftakhurohmah . Miftakhurohmah Miftakhurohmah MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Mimi Sutrawati Mubin, Nadzirum Muhammad Fikri Hafizh Munif Ghulamahdi N Mattjik Ni Putu Pandawani Niken Nur Kasim, Niken Nur Niky Elfa Amanatillah NISA RACHMANIA MUBARIK Nur Unsyah Laili Nurfadillah NURHAJATI MATTJIK OLUFEMI JOSEPH ALABI Pratiwi, Rizky Nomi Primandani, Dhika Kintania Purnamawati, Iis Purwono Purwono Putri, Maharani Mustika R Kartika RAUF, AUNU RAYAPATI ADIKESAVULU NAIDU Rita Noveriza Rita Noveriza Rizki Haerunisa S Susanto Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sugeng Santoso Sugeng Santoso Supramana Supramana Suryo Wiyono Syaiful Khoiri TRISNA AGUNG PHABIOLA Wahyudin, Denih Wieke Mei Dina Yunita Fauziah Rahim Yunita Fauziah Rahim Yusmani Prayogo Zulfi, Fahma Zakiya