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IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin) Miftakhurohmah Miftakhurohmah; I Dewa Nyoman Nyana; Tri Asmira Damayanti; Rita Noveriza
Jurnal Penelitian Tanaman Industri Vol 23, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v23n1.2017.11-17

Mosaic disease on Indonesian patchouli associated with infection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of nucleotide and amino acid sequence similarities, 97,1 and 97,7% respectively, with um Japanese isolates. Phylogenetic tree analysis revealed that CMV from patchouli was closely related with um-Japanese isolate with 100% bootstrap value, and clustered with another CMV isolates in subgroup IB. Since the CMV subgroup I was more virulent than subgroup, it is necessary to increase the awareness of the CMV occurrence in another plant.Key words : homology analysis, phylogeny tree, nucleotides, amino acid AbstrakPenyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergejala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-CR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian.Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino

IDENTIFIKASI MOLEKULER BEAN COMMON MOSAIC VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK KUNING KACANG PANJANG Melinda .; Tri Asmira Damayanti; Sri Hendrastuti Hidayat
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Molecular identification of bean common mosaic virus associated with yellow mosaic disease on yard long bean. Bean common mosaic virus (BCMV) has been reported as one of the causal agents of yellow mosaic disease on yard long bean in West Java and Central Java. Infected plants showed mosaic, yellowing, and mixture of yellow mosaic. The research was conducted to identify the diversity of BCMV associated with yellow mosaic disease based on coat protein (CP) gene sequences. Symptomatic leaf samples were collected from yard long bean growing areas in several districts in West Java (Bogor, Cirebon, Subang, and Indramayu), and several districts in Central Java (Tegal, Klaten, Solo, Yogjakarta, Sleman, and Magelang). Molecular detection using RT-PCR method was carried out by using specific primer to BCMV which will amplify the CP gene. DNA fragment, + 860 bp in size, was successfully amplified from 8 out of 13 leaf samples, i.e samples from three villages in Bogor District (Cangkurawok, Bubulak, Bojong), and five samples from District of Cirebon, Subang, Solo, Sleman, and Tegal. Sequence analysis of those DNA fragment showed that 4 isolates (Bogor-Cangkurawok, Subang, Solo and Sleman) had the highest homology to BCMV-BlC from Taiwan, whereas 2 isolates (Cirebon and Tegal) had the highest homology to BCMVNL1 from England. Further, phyllogenetic analysis revealed that those of 4 isolates were closely related to BCMV-BlC from Taiwan based on nucleotide as well as amino acid sequences; while those other 2 isolates were closely related to BCMV-NL1 from England based on nucleotide sequences but closely related to BCMV-BlC Y from China based on amino acid sequences. Phyllogenetic analysis showed that those of 6 BCMV isolates separated in two different clusters; 4 isolates (Bogor- Cangkurawok, Subang, Solo, and Sleman) in cluster 1 together with BCMV-BlC from Taiwan, while other 2 isolates (Cirebon and Tegal) in cluster 2 together with BCMV-NL1.

IDENTIFIKASI MOLEKULER BROAD BEAN WILT VIRUS 2 (BBWV2) DAN CYMBIDIUM MOSAIC VIRUS (CYMMV) ASAL TANAMAN NILAM (POGOSTEMON CABLIN BENTH.) Miftakhurohmah .; Gede Suastika; Tri Asmira Damayanti; Rita Noveriza
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Molecular identification Broad Bean Wilt Virus 2 (BBWV2) and Cymbidium Mosaic Virus (CymMV) from patchouli plant (Pogostemon cablin Benth.). Several viruses have been reported to be associated with mosaic disease on patchouli plant in Indonesia. This study aims to identify the two viruses in patchouli cultivation in West Java by studying the molecular characterization. Mosaic symptomatic leaf samples taken from patchouli cultivation in Manoko (Bandung Barat District, West Java Province). RNA extraction was performed using Xprep Plant RNA mini kit. RNA amplification with RT-PCR technique using primers for the cp gene region of BBWV2 and CymMV. The PCR product was sent to PT. Science Genetics Indonesia to do sequencing, then analyzed nucleotide sequences. Results of RT-PCR were performed successfully obtained DNA bands with size accordance with the predictions of the primer design for BBWV2 and CymMV cp region. Further, based on nucleotide and amino acid sequence analyses, the two virus isolates were confirmed as BBWV2 and CymMV respectively. Phylogenetic analyses revealed that BBWV2 Manoko clustered with BBWV2 from Singapore (original host of Brazilian red-cloak), China (pepper) and South Korea (chili). Whereas, CymMV Manoko become one cluster with CymMV from India (Phaius sp.), Indonesia (Dendrobium), China (vanilla), Thailand (Oncidium), Hawai (Dendrobium) and South Korea Cymbidium).

PEMANFAATAN KITOSAN UNTUK PENGENDALIAN BEAN COMMON MOSAIC VIRUS (BCMV) PADA KACANG PANJANG Tri Asmira Damayanti; . Haryanto; Suryo Wiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Utilization of chitosan to control Bean common mosaic virus (BCMV) on yard long bean. Bean common mosaic virus (BCMV) is one of important viruses infecting yard long bean in Indonesia. One of efforts to control its infection is by utilizing chitosan. Thus, the aim of the conducted research was to test the effectiveness of chitosan in controlling BCMV on yard long bean. Concentration of chitosan tested were 0.1% and 1.0% and it was applied as seed treatment (PB), leaf spraying before (SB) and after (ST) mechanical inoculation of BCMV. Incubation period, disease incidence and severity, peroxidase enzyme activity, and BCMV titer were measured as observation parameters. Incubation period of all treated plants were longer as compared with untreated control plants. Symptoms of treated plants at concentration 0.1% (SB and ST) showed mild to severe symptom and leaf malformation, while at concentration 1.0% showing milder symptoms and some of tested plants remain symptomless. Treated plants showed lower peroxidase enzyme activity and significantly lower BCMV titer as compared with that of untreated control plants. The lowest to highest percentage of virus inhibition exhibited by ST0.1, SB0.1, PB0.1, PB1, SB1 and ST1 treatments with virus relative inhibition level ranged 75.94%-86.07% and severity inhibition level ranged 39.55%-64.63%.

Aktivitas Antivirus Beberapa Ekstrak Tanaman terhadap Bean Common Mosaic Virus strain Black Eye Cowpea (BCMV-BIC) pada Kacang Panjang Tri Asmira Damayanti; Martha Theresia Panjaitan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTAntivirus actitivity of several plant extracts against Bean common mosaic virus strain Black eye cowpea (BCMV-BlC) on Yard long bean. Bean common mosaic virus (BCMV) is an important virus on yard long bean and it is difficult to control. One of control effort way by utilizing antiviral substances of plant origin. The research was done to select and test the effectiveness of plant extracts in suppressing BCMV infection on yard long bean. Twenty two plant extracts were selected by (1) spraying the crude extract to Chenopodium amaranticolor leaves, then plant inoculated by BCMV 1 hour after spraying, and (2) mixturing the crude extract with sap containing BCMV, then inoculated mechanically to C. amaranticolor. Local necrotic lesion number and inhibition percentage are measured. All plant extract treatments were able to reduce Necrotic lokal lesion formation significantly compared to untreatment control. Further, fifteen plant extracts were selected to test their effectiveness in controlling BCMV on yard long bean in green house trial. The results showed that except geranium and red ginger treatment, other extract treatments were able to reduce significantly the disease incidence and severity, symptoms, and BCMV titer, respectively. Among tested extracts, Bougainvillea spectabilis, Mirabilis jalapa, and Celosia cristata are the most effective crude extracts in suppressing BCMV infection.

IDENTIFICATION AND CHARACTERIZATION OF CARMOVIRUS ON CARNATION (Dianthus caryophyllus L.) IN WEST JAVA, INDONESIA Erniawati Diningsih; Gede Suastika; Tri Asmira Damayanti; Slamet Susanto
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.523

Carnation has a highly economic demand of cut flower in Indonesia. Field observations in West Java Indonesia was conducted in order to find the typical mottle symptoms that was a suspect caused by a virus disease. Identification of the virus was respectively conducted by performing ELISA test with four anti sera and characterizations held by bioassay, observing of virion particles, detecting of nucleic acid by RT-PCR and nucleotide sequencing. Total of 403 samples were collected from plants with or no virus-like symptoms. Among those all tested, 83% were found to be infected by Carnation mottle virus (CarMV), but negatively against Carnation ringspot virus (CRSV), Carnation laten virus (CLV), and Carnation vein mottle virus (CVMV) antisera. By mechanical inoculation, the virus was able to infect systemically Cenopodium quinoa and locally infect on others. However on Phalaenopsis sp and Gomprena globosa, there was symptompless found. The isometric CarMV particles size was approximately 30 nm. RT-PCR using specific primers of CP gene of CarMV successfully amplified a DNA sized 1000 bp. CarMV West Java Indonesian (Idn-WJ) isolates possessed the highest nucleotide and amino acid homology with CarMV from Spain and was in the same cluster with CarMV from China, Taiwan and Israel.

Identification of Quorum Quenching Bacteria and Its Biocontrol Potential Against Soft Rot Disease Bacteria, Dickeya dadantii Syaiful Khoiri; Tri Asmira Damayanti; Giyanto Giyanto
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.633

Dickeya dadantii is one of newly found bacteria causing soft rot on orchids in Indonesia. Infected plants showed severe rot rapidly only in few days. An effort to control the bacteria was conducted by utilizing selected quorum quenching (QQ) inducer bacteria which produce AHL-lactonase by aiiA gene. The aims of this research were to screen and identify of quorum quenching bacteria, and also assayed their biocontrol potential ability against D. dadantii in laboratory. The screening of QQ bacteria was achieved using the anti-QS test, anti-microbial activity, and detection of aiiA gene using specific primer. The determination of the ability against D. dadantii was done using the soft rot assay on potato and orchid. Among thirty one bacteria isolates screened, four isolates (in succession namely B37, BT2, GG3, and GG6) were selected to control D. dadantii. All of these bacteria showed QQ ability to suppress the virulence of D. dadantii infection on orchids, significantly. Based on nucleotide sequences of 16S ribosomal RNA, those of bacteria isolates had the highest identity with Brevibacillus brevis, Bacillus cereus ATCC14579, Bacillus cereus ATCC14579 and Bacillus thuringiensis ATCC 10792. Brevibacillus brevis was reported for the first time as QQ bacteria in this study.

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin) Miftakhurohmah Miftakhurohmah; I Dewa Nyoman Nyana; Tri Asmira Damayanti; Rita Noveriza
Jurnal Penelitian Tanaman Industri Vol 23, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v23n1.2017.11-17

Mosaic disease on Indonesian patchouli associated with infection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of nucleotide and amino acid sequence similarities, 97,1 and 97,7% respectively, with um Japanese isolates. Phylogenetic tree analysis revealed that CMV from patchouli was closely related with um-Japanese isolate with 100% bootstrap value, and clustered with another CMV isolates in subgroup IB. Since the CMV subgroup I was more virulent than subgroup, it is necessary to increase the awareness of the CMV occurrence in another plant.Key words : homology analysis, phylogeny tree, nucleotides, amino acid AbstrakPenyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergejala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-CR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian.Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino

DETEKSI SECARA SEROLOGI DAN MOLEKULER BEBERAPA JENIS VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK TANAMAN NILAM (Pogostemon cablin Benth) MIFTAKHUROHMAH MIFTAKHUROHMAH; GEDE SUASTIKA; TRI ASMIRA DAMAYANTI
Jurnal Penelitian Tanaman Industri Vol 19, No 3 (2013): September 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v19n3.2013.130-138

ABSTRAKPenyakit mosaik pada tanaman nilam disebabkan oleh beberapajenis virus, yaitu Potyvirus, Potexvirus, Cucumber mosaic virus (CMV),dan Broad bean wilt virus 2 (BBWV2). Penelitian ini bertujuan untukmengidentifikasi secara serologi dan molekuler virus-virus yangberasosiasi dengan gejala mosaik pada nilam di KP. Manoko, KP. Cicurugdan lahan petani di Cijeruk. Sampel daun nilam baik yang menunjukkangejala mosaik atau pun tidak diambil dari setiap lokasi penanamanmasing–masing sebanyak 30 sampel. Kejadian penyakit ditentukan melaluideteksi serologi dengan Direct-ELISA dan Indirect-ELISA terhadap sampelmenggunakan empat antiserum, yaitu CMV, Cymbidium mosaic virus(CymMV), Potyvirus, dan BBWV2. Deteksi molekuler dengan RT-PCRdilakukan untuk mengonfirmasi virus baru yang ditemukan. Hasilpenelitian menunjukkan bahwa gejala infeksi virus yang ditemukan padanilam bervariasi, yaitu mosaik lemah, mosaik kuning hijau, mosaik denganpenebalan, mosaik dengan malformasi daun, dan bintik kuning. Secaraserologi, kejadian virus pada setiap kebun bervariasi. Di KP Manoko,Potyvirus dan BBWV2 lebih dominan (100%) dibandingkan CymMV. DiKP Cicurug, kejadian Potyvirus dan CMV terlihat lebih dominan (83,3 dan80%) dibandingkan BBWV2 dan CymMV, sedangkan di Cijeruk, BBWV2lebih dominan (90%) dari Potyvirus (50%) dan CMV (13,3%). Hasil RT-PCR dengan primer degenerate BBWV, diidentifikasi BBWV2 padasampel daun nilam dari Manoko, Cicurug, dan Cijeruk, sedangkan denganprimer general Potexvirus, diidentifikasi CymMV hanya dari sampel daunnilam dari asal Manoko. Hasil penelitian ini merupakan laporan pertamatentang BBWV2 dan CymMV pada tanaman nilam di Jawa Barat yangmengindikasikan bahwa virus merupakan kendala utama pada perbenihannilam yang harus segera diatasi.Kata kunci: BBWV2, CymMV, mosaik, Pogostemon cablin Benth, PCRABSTRACTMosaic symptoms on patchouli plant are associated with severalviruses, i.e. Potyvirus, Potexvirus, CMV, and BBWV2. The objective ofthe study was to detect virus(es) associated with mosaic symptoms onpatchouli at the the patchouli seed nurseries, in Manoko, Cicurug, andCijeruk. Thirty leaf samples either showing typical symptomatic mosaic orasymptomatic were taken from each location. Serological testing byDirect-ELISA and Indirect-ELISA using four antisera namely CMV,Cymbidium mosaic virus (CymMV), Potyvirus, and BBWV2 was carriedout to test the incidence of each virus. Molecular detection by RT-PCR wasperformed to confirm the new virus(es). The results showed that symptomsof virus infection were found vary, i.e. weak mosaic, green yellow mosaic,mosaic with thickening, mosaic with leaf malformations, and yellow spot.Based on the serological detection, virus(es) incidence varied at each seednurseries. In Manoko, Potyvirus, and BBWV2 were more dominant(100%) compared with CymMV. In Cicurug, Potyvirus and CMV weremore dominat (83.3 and 80%) compared with BBWV2 and CymMV.While in Cijeruk, BBWV2 was the most dominant (90%) than Potyvirus(50%) and CMV(13.3%). Result of RT-PCR with degenerate primers pairof BBWV was succesfully identified BBWV2 from Manoko, Cicurug, andCijeruk samples, whereas by using Potexvirus general primary, CymMVwas identified only from Manoko samples. BBWV2 and CymMV werefirst reported to infect patchouli in West Java. The result indicate thatvirus(es) are the major constraint on patchouli seed that should bemanaged immediately.Key words: BBWV2, CymMV, mosaic, Pogostemon cablin Benth, PCR

Respons Varietas Kedelai Unggul terhadap Cucumber mosaic virus Strain Soybean (CMV-S) Yunita Fauziah Rahim; Tri Asmira Damayanti; Munif Ghulamahdi
Buletin Palawija Vol 18, No 2 (2020): Buletin Palawija Vol 18 No 2, 2020
Publisher : Balai Penelitian Tanaman Aneka Kacang dan Umbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bulpa.v18n2.2020.p105-112

ABSTRAK Cucumber mosaic virus strain Soybean (CMV-S) merupakan salah satu jenis virus terbawa benih yang menyebabkan penurunan produksi kedelai dan dominan menginfeksi kedelai di Jawa. Penggunaan varietas kedelai unggul tahan merupakan cara terbaik mengendalikan infeksi virus. Namun, varietas kedelai unggul hasil pemuliaan yang ada saat ini umumnya untuk peningkatan produksi dan belum memprioritaskan ketahanan terhadap penyakit virus, sehingga diperlukan pengujian respons ketahanan varietas terhadap CMV-S. Varietas unggul yang diuji ketahanannya dalam percobaan di rumah kaca yaitu Argomulyo, Anjasmoro, Burangrang, Cikuray, Detam 1, Detam 2, Grobogan, Sinabung, dan Wilis. Respons ketahanan terhadap CMV-S diukur berdasarkan parameter penyakit (periode inkubasi, tipe gejala, indeks keparahan penyakit, titer virus) dan parameter pertumbuhan tanaman (tinggi tanaman, jumlah bunga, indeks sensitivitas cekaman). Data percobaan dianalisis secara deskriptif untuk parameter penyakit dan pertumbuhan tanaman. Hasil penelitian menunjukkan bahwa periode inkubasi, ekspresi gejala, keparahan dan titer virus CMV-S bervariasi antarvarietas. Infeksi CMV-S dapat menghambat pertumbuhan tanaman pada semua varietas dan jumlah bunga yang lebih sedikit pada varietas Argomulyo, Anjasmoro, Grobogan, Sinabung, dan Wilis. Varietas Grobogan dikategorikan rentan, varietas Argomulyo, Anjasmoro, Cikuray, Sinabung, dan Wilis termasuk toleran, dan varietas Burangrang, Detam 1, dan Detam 2 termasuk tahan terhadap infeksi CMV-S. Indeks sensitivitas cekaman dari varietas kedelai yang diuji beragam, yang menunjukkan perbedaan genetik antarvarietas dalam merespons infeksi CMV-S. Tiga varietas unggul tahan (Burangrang, Detam 1, dan Detam 2) dapat digunakan sebagai tetua pada perakitan varietas tahan terhadap CMV-S.

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