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Identifikasi Mutasi FecX Pada Gen BMP15 dan Pengaruhnya Terhadap Sifat Prolifik pada Kambing Lokal di Kabupaten Lombok Barat Rahmat Agus Hidayat; Sulaiman Ngongu Depamede; Maskur Maskur
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.638 KB) | DOI: 10.29303/jitpi.v1i1.3

Abstract

The aims of this study were to identify the mutations of FecX gene in the local goats and to analyze its polymorphism as well as its influence on the prolific nature of the local goats in West Lombok Regency, Indonesia. The study was conducted in the Immunobiology Laboratory of Mataram University, using DNA isolated from 100 blood samples of local female goats which have given birth of once to three times. The methods used were PCR-RFLP method and the PCR products were digested with HinfI restriction enzyme (G|ANTC) then analyzed visually based on DNA banding patterns on 2% agarose gels. The frequency of allele and genotype obtained, were then analyzed through a comparison with the secondary data of litter size obtained from the local goat keepers information. The results showed that the gene mutation of FecXG produced two alleles: "wild-type" (+) sized of 110 bp and 31 bp, and the mutant allele (G) of 141 bp with the allele frequency of 0,965 and 0,035 respectively. Combinations of alleles in the gene BMP15 produced two genotypes, namely (a) genotype ++ (110 bp/110 bp) with a frequency of 0.93, with the average litter size of 1.59 ± 0.319, and (b) genotype G + (141bp/110 bp), with a frequency of 0.07 and with the average litter size of 1.65 ± 0.202. The results of this study indicated that mutation occurred in BMP15 gene, i.e. FecXG gene, the gene responsible for the prolificacy of animals studied. Furthermore there was a correlation between polymorphism of FecXG gene and the prolific nature of the local goats, which was predicted to lead the divergence in litter size of each local goat genotype
Perubahan Status Fisiologis dan Bobot Badan Sapi Bali Bibit yang Diantarpulaukan dari Pulau Lombok ke Kalimantan Barat Ambius Anton; Lalu Muhammad Kasip; Lalu Wira Pribadi; Sulaiman Ngongu Depamede; A. Rai Somaning Asih
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.521 KB) | DOI: 10.29303/jitpi.v2i1.17

Abstract

The aims of this study were to investigate the changes of physiology and body weight of Bali cattle heiferstransported from Lombok Island to West Kalimantan. This was a case study on effect of transportation by shipof Bali cattle heifers from the Lombok Island to West Kalimantan. Twenty Bali cattle heifers with the initialbody weight of 123.28±11.00 kg were observed in this study. Samples were determined using the purposivesampling of the total 378 cattle during transportation. Body temperature, respiratory, pulse, hemoglobin,hematocrit, blood glucose and body weight of the heifers were measured prior to transportation (T0) and aftertransportation (T1). The temperature and humidity of the environment in the ship during the transportationwere also noted. Data were analyzed by using descriptive analysis (Arithmetic means) and the differenceswere tested using T-Test. The results show that livestock transportation had some negative effects on theperformances of the heifers. The average of the ship paddock temperature and the humidity during thetransportation period were 28.83±0.860C and 72.57±4.08%, respectively. The physiological status of bodytemperature; pulse; respiratory and blood glucose concentrations increased (P>0.05) by 0.600C; 8.25time/min; 7.6 times/min and 8.7 mg/dL, respectively. Whilst, the hematocrit levels, hemoglobin concentrationand body weight of the heifers decreased significantly (P>0.05) by 2.73%; 0.90 g/dL; and 21.23 kg/head,respectively. The high decrease of the heifers’ body weight changes (17.22%) was suspected to be due to lackof feed and water intakes during transportation. It is suggested that the heifers should have enough feed andwater consumption during tranportation for reducing stress.
Infection and Distribution of Liver Fluke (Fasciola Sp) Zoonotic Parasite on Cattle in Central Lombok and East Lombok Districts Wayan Wariata; Made Sriasih; Anwar Rosyidi; Muhamad Ali; Sulaiman N. Depamede
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 5 No 2 (2019): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.317 KB) | DOI: 10.29303/jitpi.v5i1.55

Abstract

Salah satu faktor yang sangat mempengaruhi populasi dan produksi ternak adalah masalah penyakit dan parasit. Gejala serangan parasit seringkali tidak tampak dan kadang-kadang petani kurang menyadarinya. Cacing hati (Fasciola sp.) merupakan parasit zoonosis yang dapat menginfeksi berbagai macam hewan ternak ruminansia terutama sapi. Fasciolamemiliki dua spesies utama yaitu hepatica dan gigantica. Penyebaran Fasciola sp. adalah daerah beriklim tropis basah yaitu di Asia dan Afrika. Fasciola sp. yang sering dijumpai di Indonesia adalah spesies gigantica, sedangkan spesies hepatica sering terdeteksi pada sapi-sapi impor. Untuk mengetahui infeksidan tingkat penyebaran Fasciola giganticadan Fasciola hepaticapada ternak sapi di kabupaten Lombok Tengahdan Lombok Timurmaka dilakukan survei, pengamatan dan pengambilan sampel organ hati sapi di pasar-pasar daging yang ada pada dua daerah tersebut. Pada masing-masing daerah diambil 3 lokasi pasar daging, yaitu di kabupaten Lombok Tengah: pasar Praya, pasar Mantangdan pasar Kopang; di kabupaten Lombok Timur: pasarSelong, pasarMasbagikdan pasarTerara. Pada masing-masing pasar, pengamatan dan pengambilan sampel diulang sebanyak 3 kalipada 3 orang pedagang.Selanjutnya sampel diamati di laboratorium untuk mengetahui adanya cacing hati dan kerusakan hati sapi. Hasil penelitian menunjukkan bahwa, sapi-sapi dan daging sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur terinfeksi parasit cacing hati (Fasciola sp.), dengan tingkat infeksi yang masih rendah, yaitu antara 1,3 sampai 2,3. Di samping itu, warna dan tekstur hati sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur relatif masih baik dan layak dikonsumsi.