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All Journal Tropical Medicine Journal Biospecies Biosfer: Jurnal Tadris Biologi Tadris: Jurnal keguruan dan Ilmu Tarbiyah Bioedukasi: Jurnal Pendidikan Biologi Biota Biology, Medicine, & Natural Product Chemistry Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Bio Ilmi: Jurnal Pendidikan Agriprima : Journal of Applied Agricultural Sciences BIOEDUSCIENCE JMM (Jurnal Masyarakat Mandiri) Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Medika Malahayati BIOSFER : Jurnal Biologi dan Pendidikan Biologi AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Seminar Nasional Peningkatan Mutu Pendidikan Jurnal EduHealth Inornatus: Biology Education Journal Jurnal Pemberdayaan Masyarakat Gunung Djati Conference Series Organisms: Journal of Biosciences Journal Fatmawati Laboratory & Medical Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) IJIS Edu : Indonesian Journal of Integrated Science Education Jurnal Bioteknologi & Biosains Indonesia
Marlina Kamelia
Universitas Islam Negeri Raden Intan Lampung
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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RETRACTED: Isolasi dan Seleksi Enzimatis Bakteri Selulolitik Dari Limbah Media Tanam Jamur Tiram Putih (Pleurotus Ostreatus) Berbahan Serbuk Gergaji Kayu Karet (hevea brasiliensismuell. Arg) Kamelia, Marlina; Anggoro, Bambang Sri; Novitasari, Deffi
Biosfer: Jurnal Tadris Biologi Vol 9 No 2 (2018): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v9i2.2382

Abstract

Jamur tiram putih termasuk dalam kelas Basidiomycetes yang banyak dibudidaya di Indonesia. Konsumsi terhadap jamur tiram putih (Pleurotus ostreatus) makin meningkat dikalangan masyarakat, sehingga menyisakan limbah berupa media bekas penanaman jamur. Media sisa budidaya jamur tiram sejauh ini belum banyak dimanfaakan dengan baik. Media pertumbuhan jamur disebut juga baglog akan berbahaya bagi lingkungan jika dibuang begitu saja. Pemanfaatan media sisa penanaman jamur tiram ini dapat digunakan untuk memproduksi gula pereduksi yang dihasilkan oleh aktivitas mikroorganisme yang melibatkan enzim selulase. Tujuan penelitian ini yakni melakukan isolasi dan seleksi secara enzimatis dari limbah media tanam jamur tiram putih berbahan serbuk gergaji kayu karet (Hevea brasiliensis Muell. Arg). Dari hasil isolasi diperoleh 60 isolat bakteri yang berasal dari tiga jenis usia baglog yakni, 0, 2 dan 4 bulan. Isolat tersebut selanjutnya dikarakterisasi dengan screening pada media differensial CMC guna melihat aktivitas degradasi terhadap selulosa. Hasil screening diperoleh 23 isolat yang merupakan positif selulolitik ditandai dengan adanya zona bening yang terbentuk pada media CMC. Zona bening yang terbentuk merupakan indikasi aktivitas bakteri yang dapat mengunakan sumber karbon pada CMC dan mendegradasi komponen selulosa. Hasil penelitian ini menunjukkan bahwa limbah media tanam jamur tiram putih berbahan kayu karet memiliki potensi selulolitik. Isolat yang mendominasi hasil pada penelitian ini memiliki bentuk undulate dengan tepi irreguler berwarna putih serta tekstur makrokopisnya yang licin. Sedangkan, hasil pengamatan mikrokopis menunjukkan bahwa bentuk sel coccus dengan jenis Grampositif mendominasi isolat bakteri.
ANALISIS KUALITAS ES BATU BERDASARKAN KANDUNGAN COLIFORM DI KANTIN UIN RADEN INTAN LAMPUNG Analysis Of Ice Cubes Quality Based On Coliform Content In Uin Raden Intan Lampung Canteen Kamelia, Marlina; Anggoro, Bambang Sri; Sa'adah, Farida Putri
Biosfer: Jurnal Tadris Biologi Vol 9 No 1 (2018): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosf.v9i1.2890

Abstract

Es batu yang pengolahannya kurang baik dapat terkontaminasi bakteri penyebab penyakit, salah satunya kelompok bakteri coliform. Bakteri Coliform sendiri adalah mikroba yang sering ditemukan pada kotoran manusia maupun hewan. UIN Raden Intan memiliki jumlah mahasiswa yang banyak dan sebagian besar pada siang hari pergi ke kantin untuk membeli minuman yang mengandung es batu. Es batu yang tercemar bakteri coliform akan menyebabkan gangguan kesehatan seperti diare. Hal inilah yang mendasari mengapa perlu diketahui keberadaan bakteri coliform dalam es batu dari berbagai kantin yang ada di UIN Raden Intan Lampung. Jenis penelitian ini adalah eksperimental yang dilaksanakan dari bulan April sampai dengan Mei 2017. Seluruh kantin yang ada dan satu depot penjual diambil sampel es batunya. Sampel tersebut kemudian diuji kandungan bakteri coliformnya menggunakan dua jenis media diferensial, yaitu MacConkey serta Eosin Methylene Blue (EMB). Metode SPC (Standar Plate Count) digunakan untuk menghitung jumlah bakteri yang tumbuh pada media. Hasil perhitungan jumlah bakteri pada sampel yang telah dilakukan menunjukkan bahwa 95% positif terkontaminasi bakteri coliform. Artinya hanya ada satu sampel saja yang bebas dari kontaminasi bakteri coliform.
The Vitamin C Potential of Sorrel Tree (Averrhoa bilimbi) as LATEX Coagulant Anam, Khairul; Kamelia, Marlina; Faridah, Indah
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.7877

Abstract

This research aims to determine the vitamin C potential of tree sorrels (Averrhoa bilimbi) as a natural latex coagulant. The researchers employed the Completely Randomized Design (CRD) method. The method consisted of three repetitions with five treatments, namely negative control (without clotting) and positive control, by administering tree sorrel extract (Averrhoa bilimbi) with a volume of 5 ml, 10 ml, 15, and 20 ml. The researchers tested the vitamin C levels using UV-VIS Spectrophotometry DR 6000 with a wavelength of 265 nm to 271 nm. Furthermore, the data were analyzed using the One-Way ANOVA statistical test and the LSD to determine the most effective volume as a latex coagulant. The quantitative data were the clotting time and latex weight. The results showed that tree sorrel extract could be used as a latex coagulant. The fastest duration (1.18 minutes) and the highest weight (34.30 grams) of latex were found in the 20 ml volume. At the same time, the lowest yield was at a volume of 5 ml with a clotting time of 5.4 minutes and a weight of 18.62 grams.
Students' Critical Thinking Skills in Islamic Schools: The Effect of Problem-Based Learning (PBL) Model Kamil, Badrul; Velina, Yessy; Kamelia, Marlina
Tadris: Jurnal Keguruan dan Ilmu Tarbiyah Vol 4 No 1 (2019): Tadris: Jurnal Keguruan dan Ilmu Tarbiyah
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/tadris.v4i1.4212

Abstract

Industrial Revolution 4.0 requires schools to produce graduates who are not only good in memorizing various materials, but also must have the skills to think critically. Problem-Based Learning (PBL) is one of the learning models that is deemed good in training students' critical thinking skills, including students in Islamic schools. The purpose of this research is to determine the effect of problem-based learning on students' critical thinking skills in Islamic schools. This type of research is a quasi-experimental with random sampling as the sampling technique. The research sample was 66 students. Data collection uses instruments in the form of description tests that are adjusted to the indicators of critical thinking skills. The research analysis technique used was the t-test to determine the effect of the problem-based learning model on students' critical thinking skills. The result of the research shows that the average score of the class where the critical thinking skills through PBL are applied is higher than the class that does not apply PBL. The result of the data analysis using the t-test obtained that the value of tcount bigger ttable is 4.119 > 1.997 with a significance level of 5 %, so it is said that the PBL model influences students' critical thinking skills. It is suggested to make PBL learning becomes a mandatory choice to be applied in the learning process with the learning steps that have an impact on the students' thinking process.
Antioksidan Ekstrak Etanol Buah Kecipir Dengan Metode 1,1-Diphenyl-2-Picrylhidrazyl (DPPH) Widiani, Nurhaida; Irma, Putri; Kamelia, Marlina
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 2 No. 2 (2022): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v2i2.12872

Abstract

The fruit of Psophocarpus tetragonolobus L thought to have an antioxidant activity such as seeds and leaves. Ethanol extract from winged bean contains saponins, flavonoids, polyphenols, steroids, and terpenoids. This study aims to determine the antioxidant activity of the ethanol extract of winged bean fruit using the DPPH (1,1-diphenyl-2-picrylhydrazyl). The antioxidant activity test of winged bean extract was carried out at concentrations of 10 ppm, 50 ppm, 100 ppm, and 150 ppm. Added with DPPH (50 ppm) of winged bean extract. Vitamin C was used as a positive control. Absorbance measurement to determine the antioxidant activity of winged bean using a UV-Vis spectrophotometer at a maximum wavelength of 517 nm. The results of this study obtained a qualitative color change in both the winged bean extract and vitamin C. The IC50 value winged bean extract was 98.3229 ppm and included strong antioxidant activity based on the Blois classification.AbstrakBuah kecipir (Psophocarpus tetragonolobus L) diduga memiliki aktivitas antioksidan seperti halnya biji dan daunnya. Ekstrak etanol dari buah kecipir mengandung saponin, flavonoid, polifenolat, steroid dan terpenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak etanol buah kecipir dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Ekstrak buah kecipir didapat dengan cara maserasi menggunakan pelarut etanol. Uji aktivitas antioksidan ekstrak buah kecipir dilakukan pada konsentrasi 10 ppm, 50 ppm, 100 ppm, dan 150 ppm. Ekstrak buah kecipir ditambah dengan DPPH (50 ppm). Vitamin C digunakan sebagai kontrol positif. Pengukuran absorbansi untuk mengetahui aktivitas antioksidan buah kecipir menggunakan spektrofotometer UV-Vis pada panjang gelombang maksimum 517 nm. Hasil penelitian ini didapatkan perubahan warna secara kualitatif baik pada ekstrak buah kecipir dan vitamin C. Nilai IC50 ekstrak buah kecipir senilai 98,3229 ppm dan termasuk memiliki aktivitas antioksidan kuat.
Formulasi Wolffia arrhiza dan Ransum Komersial PF 500 Pada Pertumbuhan Ikan Nila (Oreochromis niloticus) Kamelia, Marlina; Rudini, Mahmud; Zahra, Zackia Irina; Zein, Suharno
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 2 No. 2 (2022): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v2i2.13826

Abstract

Consumption of fish farming activities is hampered by one of the main obstacles, namely the increase in the price of commercial rations, while the ration needs themselves can reach 50-70% of operational costs. Therefore, efforts are needed to develop alternative rations that can be obtained naturally and are available in the long term. The aquatic plant Wolffia arrhiza is thought to be substituted in livestock rations. This study aimed to determine of Wolffia (Wolffia arrhiza) formulation and PF 500 commercial ration on the growth of tilapia (Oreochromis niloticus). This type of research is quantitative with a completely randomized design consisting of 5 treatments and 3 repetitions. Research parameters include; weight, length, FCR, SGR, temperature, and pH. This study showed that the use of fresh Wolffia as a ration for tilapia gave a good effect. Fresh Wolffia formulations and commercial rations PF 500 showed the best growth results in P3 treatment with 20% fresh Wolffia and 80% commercial rationsAbstrakKegiatan budidaya ikan konsumsi terhambat oleh salah satu kendala utama yaitu naiknya harga ransum komersial, sedangkan kebutuhan ransum sendiri bisa mencapai 50-70% dari biaya operasional. Oleh karena itu diperlukan upaya pengembangan ransum alternatif yang bisa diperoleh secara alami serta tersedia dalam jangka panjang. Tanaman air Wolffia arrhiza diduga bisa disubtitusikan dalam ransum ternak. Penelitian ini bertujuan untuk untuk mengetahui formulasi wolffia (Wolffia arrhiza) dan ransum komersial PF 500 pada pertumbuhan ikan nila (Oreochromis niloticus). Jenis penilitian ini kuantitatif dengan Rancangan Acak Lengkap terdiri dari 5 perlakuan dan 3 kali pengulangan. Parameter penelitian meliputi; berat, panjang, FCR, SGR, suhu, dan pH. Penelitian ini menunjukan penggunaan wolffia segar sebagai ransum ikan nila memberikan pengaruh yang baik. Formulasi wolffia segar dan ransum komersial PF 500 menunjukan hasil pertumbuhan paling baik pada perlakuan P3 dengan perlakuan 20% wolffia segar dan 80% ransum komersial
Analisis Kualitas Teh Kombucha Berdasarkan Jenis Teh Yang Digunakan Kamelia, Marlina; Winandari, Ovi Prasetya; Supriyadi, Supriyadi; Meirina, Meirina
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 3 No. 1 (2023): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v3i1.16391

Abstract

Tea is a type of plant that is often used as an ingredient in functional drinks. Based on the method of processing the leaves after harvest, they are divided into black tea, white tea, green tea, yellow tea, oolong tea, and pu erh. Kombucha tea is one example of a functional tea-based drink. Kombucha tea is made by fermenting a tea solution that has been given sugar using a microbial starter in the form of SCOBY (Symbiotic Culture of Bactery and Yeast). This fermentation is generally for 8-12 days. This study aims to analyze the quality of kombucha tea with different tea base ingredients. This type of research is a quantitative experimental design with a completely randomized design (CRD) using 4 variations of tea, namely white tea, black tea, green tea, and oolong tea. The parameters analyzed included total acid (%), pH value, vitamin C content (mg/ml), antioxidant activity, thickness of nata, and organoleptic test. This study shows that different types of tea affect the quality of kombucha tea. The highest levels of vitamin C in white tea were 5,81 mg/ml; The highest total acid content in green tea was 1.44%; The lowest pH in black tea is 3.51; the highest antioxidant activity in white tea was 92,29%; the highest thickness of nata in black tea was 0.72 cm; Organoleptic test for the most preferred aroma was white tea (6.9), the most preferred color was green tea (7.54), while the most preferred flavor was green tea (7.24). AbstrakTeh merupakan salah jenis tanaman yang sering digunakan sebagai bahan minuman fungsional.  Teh berdasarkan cara memproses daunnya setelah panen dibedakan menjadi teh hitam (black tea), teh putih (white tea), teh hijau (green tea), teh kuning (yellow tea), teh oolong (oolong tea), dan teh pu erh (pu erh tea). Teh kombucha adalah salah satu contoh minuman fungsional berbahan dasar teh. Teh kombucha dibuat dengan memfermentasi larutan teh yang telah diberi gula menggunakan starter mikroba berupa SCOBY (Symbiotic Culture of Bactery and Yeast). Fermentasi ini umumnya selama 8-12 hari. Penelitian ini bertujuan untuk menganalisis kualitas teh kombucha dengan bahan dasar teh yang berbeda. Jenis penelitian ini adalah eksperimental kuantitatif yang dirancang dengan Rancangan Acak Lengkap (RAL) menggunakan 4 variasi teh yaitu teh putih, teh hitam, teh hijau, dan teh oolong.  Parameter yang dianalisis meliputi total asam (%), nilai pH, kadar vitamin C (mg/ml),  aktivitas antioksidan, ketebalan nata, serta uji organoleptik.  Penelitian ini menunjukkan bahwa jenis teh yang berbeda mempengaruhi kualitas teh kombucha.  Kadar vitamin C tertinggi pada teh putih sebesar 5,81 mg/ml; kadar total asam tertinggi pada teh hijau sebesar 1,44 %; pH terendah pada teh hitam sebesar 3,51; aktivitas antioksidan tertinggi pada teh putih sebesar 92,29%; ketebalan nata tertinggi pada teh hitam sebesar 0,72 cm; uji organoleptik untuk aroma paling disukai yaitu teh putih (6,9), warna paling disukai yaitu teh hijau (7,54), sedangkan rasa  yang paling disukai yaitu teh hijau (7,24). 
Dampak Toksik Bahan Bakar Minyak (BBM) Premium Terhadap Pembentukan Mikronukleus pada Akar Bawang Merah (Allium cepa): Analisis Konsentrasi dan Waktu Pemaparan Kamelia, Marlina; Hendras Restuti, Ayesha Utari; Satiyarti, Rina Budi
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 4 No. 1 (2024): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v4i1.21472

Abstract

Fuel Oil (BBM) is fuel that originates and or is processed from petroleum. Premium contains tetra-ethyl-lead containing lead with an octane rating of 88. Lead compounds are carcinogenic chemicals that can trigger aberrations and chromosomal damage that can be expressed in the form of micronuclei. Another compound commonly found in gasoline is BTEX. BTEX is an organic compound in petroleum derivatives that has been specifically studied to exhibit toxic properties. The purpose of this study was to determine the presence of micronuclei as a result of premium exposure. The study was conducted using a completely randomized design (CRD) with 2 factors, namely the premium concentration of P1(5%), P2 (10%), P3 (15%), P4 (20%) P5 (25%) and the length of exposure time of T1 (24 hours)), T2 (48 hours), T3 (72 hours). Statistical analysis is Two Way ANOVA analysis, further test Tukey. The results showed that the premium concentration had an effect on the formation of shallot (Allium cepa) root micronucleus, the significance value was 0.000 (p<0.05), the exposure time had an effect on the formation of the shallot (Allium cepa) root micronucleus. The significance value was 0.014 (p<0.05), the interaction between concentration and duration of exposure did not affect the formation of root micronucleus of shallot (Allium cepa) 0.999 (p>0.05). The higher the concentration of pollutants used, the more the number of micronucleus.AbstrakBahan Bakar Minyak (BBM) adalah bahan bakar yang berasal dan atau diolah dari minyak bumi. Premium mengandung tetra-etil-lead yang mengandung timbal dengan nilai oktan 88. Senyawa timbal merupakan zat kimia yang bersifat karsinogenik yang dapat memicu aberasi dan kerusakan kromosom yang dapat terekspresikan dalam bentuk mikronukleus. Senyawa lain yang biasa ditemukan dalam bensin adalah BTEX. BTEX adalah senyawa organik dalam turunan minyak bumi yang telah dipelajari secara khusus menunjukan sifat toksik. Tujuan penelitian ini adalah untu mengetahui adanya mikronukleus sebagai akibat pemaparan premium. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) 2 Faktor yaitu konsentrasi premium P1(5%), P2 (10%),  P3 (15%), P4 (20%) P5 (25%) dan lama waktu pemaparan T1 (24 jam), T2 (48 jam), T3 (72 jam). Analisis statistik adalah analisis Two Way ANOVA, uji lanjut Tukey. Hasil penelitian menunjukan konsentrasi premium berpengaruh terhadap pembentukan mikronukles akar bawang merah (Allium cepa) nilai signifikansi 0,000 (p<0,05), waktu pemaparan berpengaruh terhadap pembentukan mikronukles akar bawang merah (Allium cepa) nilai signifikansi 0,014 (p<0,05), interaksi antara konsentrasi dan lama aktu pemaparan tidak berpengaruh terhadap pembentukan mikronukles akar bawang merah (Allium cepa) 0,999 (p>0,05). Semakin tinggi konsentrasi pencemar yang digunakan jumlah mikronukleus semakin banyak.
Analysis of Proximates, Organoleptics, and Saving Power of Fortified Wet Noodles Curcuma domestica and Alpinia purpurata K. Schum. Kamelia, Marlina; Kurnia, Aniza; Supriyadi
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 5 No. 1 (2025): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/mvjad164

Abstract

The storability of wet noodles is generally very low because it has a high water content. This can cause losses to the wet noodle sellers so that these problems are often overcome using chemicals as preservatives. But this in the long run can cause health problems. Therefore, the aim of this study is to increase the shelf life of wet noodles by using natural preservatives in combination with Curcuma domestica and Alpinia purpurata K. Schchum. This research is an experiment that was designed with a Completely Randomized Design (CRD) with 4 treatments, namely control (P0) and 3 combinations of Curcuma domestica and Alpinia purpurata K. Schchum 5%: 15% (P1); 10%: 10% (P2); and 15%: 5% (P3), each of which was repeated 4 times. Wet noodle samples were then performed Proximate Test, Organoleptic Test, and Microbiology Test (TPC and Mold/Yeast). The data obtained were then tested for ANOVA and continued with a LSD test with a value of α = 5%.  The results of the wet noodle test with the addition of a combination of CCurcuma domestica and Alpinia purpurata K. Schchum showed proximate levels according to the SNI stipulations. The best organoleptic wet noodle test results are P2 treatment. In the TPC cup count results and the total mold/yeast combination of Curcuma domestica and Alpinia purpurata K. Schchum the best treatment is also found in the P2 sample which can increase the storability of wet noodles up to 48 hours which appears in the total microbial and mold / yeast does not exceed the maximum limit of the provisions SNI.
Isolation and Identification of Gut Bacteria from Mealworm (Tenebrio molitor) with Potential for Microplastic Degradation Irbah, Istiana; Kamelia, Marlina; Winandari, Ovi Prasetya
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7496

Abstract

Background: Plastic that accumulates in the environment over extended periods can fragment into smaller particles known as microplastics. The uncontrolled buildup of microplastics poses significant risks, including harmful health effects on living organisms and negative impacts on ecosystems. One promising sustainable solution under investigation is the use of gut bacteria from mealworm (Tenebrio molitor) for plastic biodegradation. This study aims to identify the gut bacterial species involved in degrading plastics ingested by mealworm fed with Polystyrene (PS), Polyethylene (PE), and Polyethylene Terephthalate (PET), and to evaluate the degradation rate exhibited by these bacteria. Methodology: Bacterial isolation was carried out from the gut of mealworm that were fed plastic for 30 days, using Mineral Salts Medium (MSM) as a selective medium, followed by testing the plastic degradation potential of the isolated gut bacteria. The microbial species involved were identified using the VITEX-2 Compact System. Findings: Bacteria isolated from the digestive tract of mealworm showed significant potential in degrading plastics. Pseudomonas aeruginosa was found in PS and PE treatments with degradation rates of 14.4% and 44.4%, respectively, while Aeromonas salmonicida was identified in the PET treatment with a degradation rate of 16.3%. Contribution: These findings highlight the role of mealworm gut microbes in degrading PS, PE, and PET, supporting their potential as eco-friendly biodegradation agents
Co-Authors Ade Lenty Hoya Adistyaningrum, Nurul Ahmad Ahmad Ahmad Ahmad Ahmad Fauzi Rosnadi Almayda, Lika Dini Anam, M. Khairul Andri Jaya Kesuma Ari Hermawan Asa Izati Aulia Novitasari Aulia Ulmillah Aulia Ulmillah Ayu Febriyani, Vicca Badrul Kamil Baika, Febta Dwi Bambang Sri Anggoro Deffi Novitasari Dian Nur Chomsyah Dina Maulina Dwijowati Aji Saputri Dwijowati Asih Saputri Farida, Farida Faridah, Indah Fathurohman Fathurohman Fathurohman Fathurohman, Fathurohman FNU Supriyadi Franciska Anes Rianti Hendras Restuti, Ayesha Utari Hening, Jeni Latri Indah Faridah Irbah, Istiana Irma Sari Irma, Putri Irwandani Irwandani Khairul Anam Khairul Anam Kurnia, Aniza Mahardika Agus Wijayanti Mahmud Rudini Maylinda, Vera Meirina Meirina Meirina, Meirina Muhammad Fahmi Erlangga Netriwati Nita Ayu Agustin Novita Nurhasanah Novitasari, Deffi Novitasari, Yeni Novitasari, Yeni Nurhaida Widiani Nurhaida Widiani Oktaviyani, Sari Ovi Prasetya Winandari Ovi Prasetya Winandari, Ovi Prasetya Puji Nurrohmah Putri Irma Rani Yosilia Rasuane Noor Reza Fahlida Rina Budi Satiyarti Rina Budi Satiyarti, Rina Budi Riyanti, Franciska Anes Rizki, Erika Puspita Ruhban Maskur Sa'adah, Farida Putri Saadah, Farida Putri Satitiningrum, Yuni Shinta Almayra Shinta Anisya Siti Fatayati Suci Ristawati Suci Wulan Pawhestri Suci Wulan Pawhestri Suci Wulan Pawhestri Suharno Zein Suharno Zein Suharno Zein, Suharno Suharno Zen Supargiyono - Supriyadi Supriyadi Supriyadi Supriyadi Suri, Intan Mulia Tantri Subo Marmanik Triawan Alkausar Umillah, Aulia Veny Endarhadi A Winda Isti Utami Yeni Novitasari Yessy Velina Zackia Irina Zahra Zahra, Zackia Irina