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All Journal Hemera Zoa Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Ilmiah Mahasiswa Veteriner The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria
Dian Masyitha
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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STUDI GAMBARAN HISTOLOGI, HISTOMORFOMETRI DAN HISTOKIMIA SEBARAN GLIKOGEN PADA OTOT DADA DAN PAHA AYAM KAMPUNG (GALLUS GALLUS DOMESTICUS) PERIODE SEBELUM DAN SETELAH MENETAS Muslim Akmal; Sultan Fadhilla Taqwa; Dian Masyitha; Erdiansyah Rahmi; M Nur Salim; Amiruddin Amiruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 3 (2022): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i3.15313

Abstract

Otot merupakan bagian utama yang penting pada tubuh yang berfungsi sebagai alat gerak aktif, termoregulasi, dan membentuk tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histologi, histomorfometri, dan histokimia sebaran glikogen pada otot dada dan paha ayam kampung periode sebelum dan setelah menetas dengan menggunakan pewarnaan Haematoksilin-Eosin (HE) dan Periodic acid-Schiff (PAS). Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam otot dada dan paha ayam. Hasil penelitian menunjukkan perkembangan jaringan yang diawali oleh sel mioblas kemudian menjadi serabut otot yang berkumpul membentuk fasikulus. Hasil pengukuran diameter fasikulus otot dada dan paha serta serabut otot dada dan paha ayam kampung hari ke-14, hari ke-20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 182,06 ± 4,71µm dan 122,16 ± 5,01µm; 371,43 ± 1,77 µmdan 173,25 ± 5,58 µm; serta 587,47 ± 20,25 µmdan 192,89 ± 5,60µm, serta untuk serabut otot 30,59 ± 1,57 µmdan  23 ± 0,23; 74,23 µm ± 0,52dan 42,47  ± 0,46 µm; 149,11 ± 0,95 µmdan 59,23 ± 0,77 µm. Hasil histokimia sebaran glikogen pada jaringan otot ayam kampung hari ke-14, hari ke-20 sebelum menetas dan hari ke-7 setelah menetas pada otot berturut-turut adalah (+), (++), (+++). Dapat disimpulkan bahwa pertumbuhan otot dada dan paha ayam kampung terdapat perbedaan nyata pada setiap kelompok umur. 
Gambaran Histologi dan Histomorfometri Ginjal Kalkun (Meleagris gallopavo) pada Tingkatan Umur Berbeda Zainuddin Zainuddin; Fachreza Oktavian Syahputri; Dian Masyitha; Siti Aisyah; Cut Dahlia Iskandar; Erdiansyah Rahmi; Lian Varis Riandi
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.17388

Abstract

ABSTRAKGinjal adalah organ ekskresi utama yang memiliki peran penting dalam proses pengeluaran sisa metabolisme. Organ ginjal memiliki perkembangan seiring dengan pertambahan umur. Penelitian ini bertujuan untuk mengetahui gambaran histologi dan histomorfometri ginjal kalkun (Meleagris gallopavo) pada tingkatan umur yang berbeda. Jumlah total sampel yang diamati adalah 18 sampel ginjal kalkun jantan yang dibagi ke dalam tiga kelompok umur yaitu 8, 16, dan 24 minggu dengan masing-masing kelompok umur terdapat enam ekor kalkun. Ginjal kemudian diproses hingga menjadi sediaan histologi dengan ketebalan 3µm, selanjutnya diwarnai menggunakan pewarnaan Hematoksilin-Eosin (HE). Berdasarkan hasil penelitian ditemukan bahwa terdapat perbedaan struktur histologi ginjal pada tiap kelompok umur. Organ pada sistem urinaria unggas terutama ginjal sudah terbentuk dan sudah menjalankan fungsinya dengan sempurna setelah menetas. Data histomorfometri ginjal kalkun yang telah dianalisis menunjukan hasil berbeda nyata (P0,05) antara ukuran diameter glomerulus dan tubulus-tubulus ginjal dari berbagai tingkatan umur. Dapat disimpulkan bahwa terdapat perbedaan perkembangan ginjal kalkun baik secara histologi maupun secara histomorfometri antara umur 8, 16 dan 24 minggu.Kata kunci: Ginjal, histologi, histomorfometri, kalkun (Meleagris gallopavo)ABSTRACTKidneys are the main excretory organs that have an important role in the process of eliminating metabolic waste. Kidneys develop along with the age. This study aimesd to observed the histological and histomorphometric features of the kidney of turkey (Meleagris gallopavo) at different age levels. Total of samples observed were 18 samples of male turkey kidneys which were divided into three age groups, namely 8, 16, and 24 weeks with six turkeys in each age group. The kidneys were then processed into a histology preparation with a thickness of 3µm, then stained using Hematoxylin-Eosin (HE) staining. Based on the results of the study it was found that there were differences in the histological structure of the kidneys in each age group. The organs in the poultry urinary system, especially the kidneys, have been formed and have performed their functions perfectly after hatching. The histomorphometric data of turkey kidneys that have been analyzed showed significantly different results (P0.05) between the diameters of the glomeruli and kidney tubules from various age levels. It can be concluded that there are differences in the development of turkey kidneys both histologically and histomorphometrically between the ages of 8, 16 and 24 weeks.Keyword: Histological, histomorfometry, kidneys, turkey (Meleagris gallopavo)
The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Ajirni Ajirni; Nuzul Asmilia; Nazaruddin Nazaruddin; Amalia Sutriana; Dian Masyitha; Muhammad Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 5, No 2 (2020): Vol. 5 (2) November 2020
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.63 KB) | DOI: 10.21157/ijtvbr.v5i2.20484

Abstract

Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).
14. Effect of Curry Leaf (Murraya koenigii) Extract to Early Spoilage of Beef Akmal Safrijal; Razali Razali; Ismail Ismail; T. Reza Ferasyi; Nurliana Nurliana; Dian Masyitha
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.4417

Abstract

This study aimed to determine early spoilage of beef after addition of curry leaf extract. The sample used is beef Semimembranosus muscle which is divided into 42 parts each weight is 5 grams. This research used experimental method which consists of three treatments with two repetitions. P1 group is without curry leaf extract (control), group P2 with curry leaf extract 25% used and group P3 with curry leaf extract 50%. The entire sample is stored in a refrigerator at 4 ° C. For 7 days early spoilage test is conducted every day at 9am and 4pm. The result showed difference early spoilage time between each treatment. In P1 positive result of early spoilage time is on the 4th day and P2 positive result of early spoilage time is on the 5th day. While in P3 has not obtained positive result of early spoilage time at 7 days storage. Based on the results of this study we concluded that curry leaf extract can slow down the early spoilage time of beef
8. The Total of Leukocytes Mice (Mus musculus) Exposed To Secondhand Smoke Extract And Given Watermelon (Citrullus vulgaris) Dewi Mauliza; Rusli Rusli; Roslizawaty Roslizawaty; Rosmaidar Rosmaidar; Rinidar Rinidar; Dian Masyitha
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4269

Abstract

This study aimed to determine the effect of the red watermelon extract can affect the number of leukocytes in mice exposed to cigarette smoke. This study was designed with a completely randomized design using a unidirectional pattern 20 male mice (Mus muscullus) were divided into 4 groups. K1 negative controls were given 0.5 ml of distilled water, K2 positive control by exposure to cigarette smoke and 0.5 ml of distilled water, K3 by exposure to cigarette smoke and red watermelon extract were given a dose of 22 mg / mouse and K4 by exposure to cigarette smoke and were given extracts of red watermelon a dose of 44 mg / mouse. Exposure to cigarette smoke and red watermelon extract conducted for 30 days, making blood on day 31. Blood sampling performed at Plexus Retroorbitalis. Furthermore, counting the number of leukocytes. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of watermelon for 30 days showed their real influence (P 0.05) to the number of leukocytes in mice. It is concluded that free radicals contained in cigarette can cause a leukocytosis, an inflammatory, oxidative stress and red watermelon extract can minimize cell damage.
GAMBARAN HISTOLOGI KELENJAR TEMBOLOK AYAM KAMPUNG,BEBEK, DAN MERPATI Zainuddin -; Dian Masyitha; Fitriani -; Firda Muharrami; Sri Wahyuni; Roslizawaty -; Mulyadi Adam
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.3002

Abstract

Penelitian ini bertujuan melihat gambaran histologi kelenjar mukosa tembolok ayam kampung (Gallus domesticus), bebek (Anser anser domesticus), dan merpati (Columba domesticus), masing-masing berjumlah 3 ekor. Sampel yang digunakan adalah tembolok dari ayam kampung, bebek, dan merpati. Jaringan tembolok masing-masing hewan diambil dan diproses secara mikroteknik dengan pewarnaan Hematoksilin-Eosin (HE) kemudian diamati di bawah mikroskop.Hasil penelitian menunjukkan ada perbedaan bentuk antara kelenjar mukosa tembolok ayam kampung dan bebek.Bentuk kelenjar mukosa tembolok ayam kampung bulat, sedangkan bebek lonjong.Baik ayam kampung maupun bebek kelenjar mukosa tembolok tersebar di lamina propia. Pada ayam kampung jumlah kelenjar mukosa tembolok lebih banyak dibandingkan kelenjarmukosa tembolok bebek. Pada merpati tidak dijumpai kelenjar mukosa tembolok di lamina propia.
18. The Effect of Sipatah-Patah (Cissus quadrangularis Salisb) Extract on Mandible Density of Ovariectomized Rats (Rattus norvegicus) Indah Maghfirah; Muhammad Jalaluddin; Dian Masyitha; Mustafa Sabri; Fadli A Gani; Herrialfian Herrialfian
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i2.4307

Abstract

The aim of this research was to identify the effect of sipatah-patah (Cissus quadrangularis Salisb) leaf extract on mandible density of ovariectomized rats (Rattus norvegicus). The samples were 12 female rats which were divided into 4 treatment groups with 3 replications. K0 consisted of ovariectomized rats without Cissus quadrangularis Salisb exstract, wheares K1, K2 and K3 consisted of ovariectomized rats  and were given Sipatah-patah extract with 500mg/kg body weight, 700/mg/kg body weight and 900mg/kg body weight for 30 days. On 31st day, rats were euthanized with cloroform and mandible bones were collected for histological preparation. Parameters observed were bone structures (osteosit, osteoblast, osteoclast and trabekula). The results showed that in K0 group, bone density was decreased and became thinner, osteoclast cells were found on trabekula, and low density of active and passive osteoblast were also detected. The treatment group (K1 and K2) showed an improvement stucture of trabekula,where the amount of osteoclast were lower than K0, as well as the amount of active osteoblast, although a lot of passive osteoblast were still found. The treatment group (K3) showed an improvement as the most dense trabekula structure with large amount of active osteoblast cell density without the existance of osteoclast compared to other grups. In this research, it would be concluded that the adding sipatah-patah extract with a dose of 900 mg/kb body weight showed a higher number of active osteoblasts and trabekula density compared to the control groups, K1, and K2 on ovariectomized mandible bone rats
20. The Histological Structure of Tegal Duck (Anas javanicus) Spleen at Different Age Nabila Latifa Hafizsha; Hamdani Budiman; Hamny Hamny; Dian Masyitha; Cut Dahlia Iskandar; Ummu Balqis; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.3522

Abstract

The aim of the  study was to determine the histological structure of tegal duck (Anas javanicus) spleen according to ages variation. One month, 2 month and 3 months of tegal duck were used as the sample of this study. The spleen of each duck was analyzed by Hematoxylin-Eosin (HE) method, then observed under light microscope. The result of this study performed there were the differences of histological structure of the ducks spleen. The capsule thickness had increased along with increasing age, which was 16.0 ± 1.0 µm; 25.3 ± 2.5 µm; and 29.0 ± 1.0 µm. The boundary between the two pulp in the tegal duck 3 months  old more obvious than 1 month or 2 months old. The diameter of white pulp had increased in the oldest duck. It can be concluded that the development of spleen histological structure was more perfect with the  increasing age of tegal duck.
Co-Authors . Darmawi . Erina . Ismail . Nurliana . Rinidar Abd. Rasyid Syamsuri Adi Firmansyah Ajirni Ajirni Akmal Safrijal Amalia Sutriana Amiruddin . Anisa Diah Ulfa Arman Sayuti Azhar Azhar Azmi Zul Budianto Panjaitan Cut Dahlia Iskandar cut nila tasmi Dasrul Dasrul Dewi Mauliza Dinda Jasmal Sikumbang Erdiansya Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erwin Erwin Fachreza Oktavian Syahputri Fachry Abda El Rahman Fadli A Gani Fadli A. Gani Fashihah Rahmah Noya Farimansysh febri ramadana Firda Muharrami Fitriani - Fitriani Fitriani Gholib Gholib gina morina Hamdan Hamdan Hamdani Budiman Hamny Sofyan Has Nita Hastitin Nel Kamta Hendri Hermawan Adinugraha Herrialfian Herrialfian Indah Maghfirah Ismail Ismail Lian Varis Riandi M Hasan M Jalaluddin M Nur Salim meutya silviana morow Muhammad Hambal Muhammad Isa Muhammad Jalaluddin Muhammad Nafis Mulyadi Adam Muslim Akmal Mustafa Sabri Nabila Latifa Hafizsha Nanda Afrizan Nazaruddin Nazaruddin Nellita Mutia Nurliana . NURLIANA NURLIANA Nuzul Asmilia Razali Razali resti aulia putri Reza Perdana Putra Rinidar Rinidar Roslizawaty - Roslizawaty Roslizawaty Rosmaidar Rosmaidar roza Agravion Rusli Rusli Sema Coumandary Siti Aisyah Sri Estuningsih Sri Wahyuni Sugito Sugito Sultan Fadhilla Taqwa syafarina hanum Syafruddin Syafruddin T. Reza Ferasyi Ummu Balqis Utari Desya Winaruddin Winaruddin Zainuddin - Zainuddin Zainuddin Zainuddin Zainuddin