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Morfologi Anatomi dan Histologi Usus Biawak Air (Varanus salvator) THE ANATOMICAL AND HISTOLOGICAL MORPHOLOGY OF INTESTINAL WATER MONITOR (VARANUS SALVATOR) Hamny .; Sri Mulyani; Dian Masyitha; Sri Wahyuni; Muhammad Jalaluddin
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.366 KB)

Abstract

The objective of this study was to determine the anatomical morphology and histology of the intestineof water monitor (Varanussalvator). Intestine organ from an adult male water monito was used in thisstudy. Perfusion was performed to flow the 4% paraformaldehyde fixative solution through the heart.Intestine was collected, then divided into six sections, followed with dipping them into 4% paraformaldehydesolution for four days, continued histological preparations and stained with haematoxylin eosin (HE). Theanatomical morphology observation was performed before the intestine was processed into histologicalpreparations by observing the outer shape of the intestine and measuring it’slength. Morphologicalobservation was made †after processing theintestinal tissue by observing each portion of the intestinalwall structure. The results showed that transverse fold were found on the outer and inner surfaces of theintestine section I-IV, while in the V-VI part of the intestine, the folds were not observed anymore. Theintestine wall consisted of four layers, such as mucosa, submucosa, tunica muscularis, and serosa. Thevilli on intestine section I-IV were relatively longer and higher, while the intestine vili in section V-VI wererelatively short. The entire mucosa in water monitor was coated with absorptive columnar epitheliumwith goblet cells. Based on the result study, it can be concluded that the water monitor has more efficientdigestive process which can be seen morphologically from the relatively short intestinal, has transversefolds on the surface of the small intestine, and does not have cecum. The intestinal wall consists of layersi.e : mucosa, submucosa, tunica muscularis, and serosa.
Struktur Histologi dan Histomorfometri Jantung Kalkun Meleagris gallopavo pada Tingkat Umur Yang Berbeda (Histological Structure and Histomorphometry of Turkey Heart Meleagris gallopavo at Different Age Levels) Reza Perdana Putra; Erdiansyah Rahmi; Dian Masyitha; Zainuddin Zainuddin; Sri Wahyuni; M Nur Salim
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 3 (2022): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i3.18087

Abstract

ABSTRAK            Jantung adalah organ sirkulasi utama yang memiliki peranan penting dalam menyuplai darah keseluruh tubuh. Jantung unggas mempunyai empat ruang yang terdiri dari dua atrium dan dua ventrikel, setiap ruang memiliki tiga lapisan dinding yang terdiri dari; epikardium, miokardium dan endokardium. Penelitian ini bertujuan untuk mendapatkan informasi tentang struktur histologi dan histomorfometri jantung kalkun pada tingkat umur yang berbeda. Sampel penelitian dibagi menjadi tiga kelompok umur (8, 16 dan 24 minggu) dan masing-masing kelompok berjumlah enam jantung kalkun. Jantung kemudian diproses hingga menjadi sediaan histologi dengan ketebalan 5 µm, selanjutnya diwarnai menggunakan pewarnaan Haematoxylin-Eosin (HE). Kemudian dilakukan pengukuran ketebalan dinding epikardium ventrikel kanan dan kiri, miokardium ventrikel kanan dan kiri serta endokardium ventrikel kanan dan kiri. Hasil penelitian didapatkan bahwa struktur histologi jantung kalkun tidak berbeda antar kelompok perlakuan. Rataan ketebalan epikardium kanan pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 177,91±40.57 µm, 838,59±223,05 µm, 1306,96±91,37 µm. ketebalan miokardium kanan pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 1245,55±78,93 µm, 1866,21±257,13 µm, 2465,98±285,82 µm. ketebalan endokardium kanan pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 19,01±1,58 µm, 28,16±5,33 µm, 26,57±2,19 µm. ketebalan epikardium kiri pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 636,31±423,61 µm, 737,19±244,58 µm, 927,43±321,2 µm. ketebalan miokardium kiri pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 5304,99±764,52 µm, 6429,6±685,49 µm, 8334,3±1166,86 µm. ketebalan endokardium kiri pada minggu ke-8, 16 dan 24 secara berturut-turut adalah 31,92±17,36 µm, 37,06±12,53 µm, 49,78±5,16 µm. Data histomorfometri yang telah di analisis menunjukkan bahwa hasil pengukuran ketebalan lapisan dinding jantung pada setiap kelompok umur ialah berbeda nyata (P0,05). Dapat disimpulkan bahwa semakin bertambah umur hewan maka ketebalan dinding akan semakin bertambah. Kata Kunci : Jantung, kalkun (Meleagris gallopavo), histologi, histomorfometri ABSTRACT            The heart is the main circulatory organ that has an important role in supplying blood throughout the body. The avian heart has four chambers consisting of two atria and two ventricles, each chamber has three layers of walls consisting of; epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of the turkey heart at different age levels. The study sample was divided into three age groups (8, 16 and 24 weeks) and each group consisted of six turkey hearts. The heart was then processed into histological preparations with a thickness of 5 µm, then stained using Haematoxylin-Eosin (HE) staining. Then the thickness of the epicardium of the right and left ventricles, the myocardium of the right and left ventricles and the endocardium of the right and left ventricles were measured. The results showed that the histological structure of the turkey heart did not differ between the treatment groups. The mean thickness of the right epicardium at weeks 8, 16 and 24, respectively, was 177.91±40.57 µm, 838.59±223.05 µm, 1306.96±91.37 µm. the thickness of the right myocardium at weeks 8, 16 and 24 respectively were 1245.55±78.93 µm, 1866.21±257.13 µm, 2465.98±285.82 µm. The thickness of the right endocardium at weeks 8, 16 and 24 were 19.01±1.58 µm, 28.16±5.33 µm, 26.57±2.19 µm, respectively. The thickness of the left epicardium at weeks 8, 16 and 24 were 636.31±423.61 µm, 737.19±244.58 µm, 927.43±321.2 µm, respectively. The thickness of the left myocardium at weeks 8, 16 and 24 were 5304.99±764.52 µm, 6429.6±685.49 µm, 8334.3±1166.86 µm, respectively. The thickness of the left endocardium at weeks 8, 16 and 24 were 31.92±17.36 µm, 37.06±12.53 µm, 49.78±5.16 µm, respectively. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the heart wall layer in each age group are significantly different (P0.05). It can be concluded that as the animal ages, the wall thickness will increase. Keyword : Heart, turkey (Meleagris gallopavo), histology, histomorphometry
STUDI GAMBARAN HISTOLOGI, HISTOMORFOMETRI DAN HISTOKIMIA SEBARAN GLIKOGEN PADA OTOT DADA DAN PAHA AYAM KAMPUNG (GALLUS GALLUS DOMESTICUS) PERIODE SEBELUM DAN SETELAH MENETAS Muslim Akmal; Sultan Fadhilla Taqwa; Dian Masyitha; Erdiansyah Rahmi; M Nur Salim; Amiruddin Amiruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 3 (2022): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i3.15313

Abstract

Otot merupakan bagian utama yang penting pada tubuh yang berfungsi sebagai alat gerak aktif, termoregulasi, dan membentuk tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histologi, histomorfometri, dan histokimia sebaran glikogen pada otot dada dan paha ayam kampung periode sebelum dan setelah menetas dengan menggunakan pewarnaan Haematoksilin-Eosin (HE) dan Periodic acid-Schiff (PAS). Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam otot dada dan paha ayam. Hasil penelitian menunjukkan perkembangan jaringan yang diawali oleh sel mioblas kemudian menjadi serabut otot yang berkumpul membentuk fasikulus. Hasil pengukuran diameter fasikulus otot dada dan paha serta serabut otot dada dan paha ayam kampung hari ke-14, hari ke-20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 182,06 ± 4,71µm dan 122,16 ± 5,01µm; 371,43 ± 1,77 µmdan 173,25 ± 5,58 µm; serta 587,47 ± 20,25 µmdan 192,89 ± 5,60µm, serta untuk serabut otot 30,59 ± 1,57 µmdan  23 ± 0,23; 74,23 µm ± 0,52dan 42,47  ± 0,46 µm; 149,11 ± 0,95 µmdan 59,23 ± 0,77 µm. Hasil histokimia sebaran glikogen pada jaringan otot ayam kampung hari ke-14, hari ke-20 sebelum menetas dan hari ke-7 setelah menetas pada otot berturut-turut adalah (+), (++), (+++). Dapat disimpulkan bahwa pertumbuhan otot dada dan paha ayam kampung terdapat perbedaan nyata pada setiap kelompok umur. 
Gambaran Histologi dan Histomorfometri Ginjal Kalkun (Meleagris gallopavo) pada Tingkatan Umur Berbeda Zainuddin Zainuddin; Fachreza Oktavian Syahputri; Dian Masyitha; Siti Aisyah; Cut Dahlia Iskandar; Erdiansyah Rahmi; Lian Varis Riandi
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.17388

Abstract

ABSTRAKGinjal adalah organ ekskresi utama yang memiliki peran penting dalam proses pengeluaran sisa metabolisme. Organ ginjal memiliki perkembangan seiring dengan pertambahan umur. Penelitian ini bertujuan untuk mengetahui gambaran histologi dan histomorfometri ginjal kalkun (Meleagris gallopavo) pada tingkatan umur yang berbeda. Jumlah total sampel yang diamati adalah 18 sampel ginjal kalkun jantan yang dibagi ke dalam tiga kelompok umur yaitu 8, 16, dan 24 minggu dengan masing-masing kelompok umur terdapat enam ekor kalkun. Ginjal kemudian diproses hingga menjadi sediaan histologi dengan ketebalan 3µm, selanjutnya diwarnai menggunakan pewarnaan Hematoksilin-Eosin (HE). Berdasarkan hasil penelitian ditemukan bahwa terdapat perbedaan struktur histologi ginjal pada tiap kelompok umur. Organ pada sistem urinaria unggas terutama ginjal sudah terbentuk dan sudah menjalankan fungsinya dengan sempurna setelah menetas. Data histomorfometri ginjal kalkun yang telah dianalisis menunjukan hasil berbeda nyata (P0,05) antara ukuran diameter glomerulus dan tubulus-tubulus ginjal dari berbagai tingkatan umur. Dapat disimpulkan bahwa terdapat perbedaan perkembangan ginjal kalkun baik secara histologi maupun secara histomorfometri antara umur 8, 16 dan 24 minggu.Kata kunci: Ginjal, histologi, histomorfometri, kalkun (Meleagris gallopavo)ABSTRACTKidneys are the main excretory organs that have an important role in the process of eliminating metabolic waste. Kidneys develop along with the age. This study aimesd to observed the histological and histomorphometric features of the kidney of turkey (Meleagris gallopavo) at different age levels. Total of samples observed were 18 samples of male turkey kidneys which were divided into three age groups, namely 8, 16, and 24 weeks with six turkeys in each age group. The kidneys were then processed into a histology preparation with a thickness of 3µm, then stained using Hematoxylin-Eosin (HE) staining. Based on the results of the study it was found that there were differences in the histological structure of the kidneys in each age group. The organs in the poultry urinary system, especially the kidneys, have been formed and have performed their functions perfectly after hatching. The histomorphometric data of turkey kidneys that have been analyzed showed significantly different results (P0.05) between the diameters of the glomeruli and kidney tubules from various age levels. It can be concluded that there are differences in the development of turkey kidneys both histologically and histomorphometrically between the ages of 8, 16 and 24 weeks.Keyword: Histological, histomorfometry, kidneys, turkey (Meleagris gallopavo)
The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Ajirni Ajirni; Nuzul Asmilia; Nazaruddin Nazaruddin; Amalia Sutriana; Dian Masyitha; Muhammad Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 5, No 2 (2020): Vol. 5 (2) November 2020
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.63 KB) | DOI: 10.21157/ijtvbr.v5i2.20484

Abstract

Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).
14. Effect of Curry Leaf (Murraya koenigii) Extract to Early Spoilage of Beef Akmal Safrijal; Razali Razali; Ismail Ismail; T. Reza Ferasyi; Nurliana Nurliana; Dian Masyitha
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.4417

Abstract

This study aimed to determine early spoilage of beef after addition of curry leaf extract. The sample used is beef Semimembranosus muscle which is divided into 42 parts each weight is 5 grams. This research used experimental method which consists of three treatments with two repetitions. P1 group is without curry leaf extract (control), group P2 with curry leaf extract 25% used and group P3 with curry leaf extract 50%. The entire sample is stored in a refrigerator at 4 ° C. For 7 days early spoilage test is conducted every day at 9am and 4pm. The result showed difference early spoilage time between each treatment. In P1 positive result of early spoilage time is on the 4th day and P2 positive result of early spoilage time is on the 5th day. While in P3 has not obtained positive result of early spoilage time at 7 days storage. Based on the results of this study we concluded that curry leaf extract can slow down the early spoilage time of beef
8. The Total of Leukocytes Mice (Mus musculus) Exposed To Secondhand Smoke Extract And Given Watermelon (Citrullus vulgaris) Dewi Mauliza; Rusli Rusli; Roslizawaty Roslizawaty; Rosmaidar Rosmaidar; Rinidar Rinidar; Dian Masyitha
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4269

Abstract

This study aimed to determine the effect of the red watermelon extract can affect the number of leukocytes in mice exposed to cigarette smoke. This study was designed with a completely randomized design using a unidirectional pattern 20 male mice (Mus muscullus) were divided into 4 groups. K1 negative controls were given 0.5 ml of distilled water, K2 positive control by exposure to cigarette smoke and 0.5 ml of distilled water, K3 by exposure to cigarette smoke and red watermelon extract were given a dose of 22 mg / mouse and K4 by exposure to cigarette smoke and were given extracts of red watermelon a dose of 44 mg / mouse. Exposure to cigarette smoke and red watermelon extract conducted for 30 days, making blood on day 31. Blood sampling performed at Plexus Retroorbitalis. Furthermore, counting the number of leukocytes. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of watermelon for 30 days showed their real influence (P 0.05) to the number of leukocytes in mice. It is concluded that free radicals contained in cigarette can cause a leukocytosis, an inflammatory, oxidative stress and red watermelon extract can minimize cell damage.
GAMBARAN HISTOLOGI KELENJAR TEMBOLOK AYAM KAMPUNG,BEBEK, DAN MERPATI Zainuddin -; Dian Masyitha; Fitriani -; Firda Muharrami; Sri Wahyuni; Roslizawaty -; Mulyadi Adam
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.3002

Abstract

Penelitian ini bertujuan melihat gambaran histologi kelenjar mukosa tembolok ayam kampung (Gallus domesticus), bebek (Anser anser domesticus), dan merpati (Columba domesticus), masing-masing berjumlah 3 ekor. Sampel yang digunakan adalah tembolok dari ayam kampung, bebek, dan merpati. Jaringan tembolok masing-masing hewan diambil dan diproses secara mikroteknik dengan pewarnaan Hematoksilin-Eosin (HE) kemudian diamati di bawah mikroskop.Hasil penelitian menunjukkan ada perbedaan bentuk antara kelenjar mukosa tembolok ayam kampung dan bebek.Bentuk kelenjar mukosa tembolok ayam kampung bulat, sedangkan bebek lonjong.Baik ayam kampung maupun bebek kelenjar mukosa tembolok tersebar di lamina propia. Pada ayam kampung jumlah kelenjar mukosa tembolok lebih banyak dibandingkan kelenjarmukosa tembolok bebek. Pada merpati tidak dijumpai kelenjar mukosa tembolok di lamina propia.
18. The Effect of Sipatah-Patah (Cissus quadrangularis Salisb) Extract on Mandible Density of Ovariectomized Rats (Rattus norvegicus) Indah Maghfirah; Muhammad Jalaluddin; Dian Masyitha; Mustafa Sabri; Fadli A Gani; Herrialfian Herrialfian
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i2.4307

Abstract

The aim of this research was to identify the effect of sipatah-patah (Cissus quadrangularis Salisb) leaf extract on mandible density of ovariectomized rats (Rattus norvegicus). The samples were 12 female rats which were divided into 4 treatment groups with 3 replications. K0 consisted of ovariectomized rats without Cissus quadrangularis Salisb exstract, wheares K1, K2 and K3 consisted of ovariectomized rats  and were given Sipatah-patah extract with 500mg/kg body weight, 700/mg/kg body weight and 900mg/kg body weight for 30 days. On 31st day, rats were euthanized with cloroform and mandible bones were collected for histological preparation. Parameters observed were bone structures (osteosit, osteoblast, osteoclast and trabekula). The results showed that in K0 group, bone density was decreased and became thinner, osteoclast cells were found on trabekula, and low density of active and passive osteoblast were also detected. The treatment group (K1 and K2) showed an improvement stucture of trabekula,where the amount of osteoclast were lower than K0, as well as the amount of active osteoblast, although a lot of passive osteoblast were still found. The treatment group (K3) showed an improvement as the most dense trabekula structure with large amount of active osteoblast cell density without the existance of osteoclast compared to other grups. In this research, it would be concluded that the adding sipatah-patah extract with a dose of 900 mg/kb body weight showed a higher number of active osteoblasts and trabekula density compared to the control groups, K1, and K2 on ovariectomized mandible bone rats
20. The Histological Structure of Tegal Duck (Anas javanicus) Spleen at Different Age Nabila Latifa Hafizsha; Hamdani Budiman; Hamny Hamny; Dian Masyitha; Cut Dahlia Iskandar; Ummu Balqis; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.3522

Abstract

The aim of the  study was to determine the histological structure of tegal duck (Anas javanicus) spleen according to ages variation. One month, 2 month and 3 months of tegal duck were used as the sample of this study. The spleen of each duck was analyzed by Hematoxylin-Eosin (HE) method, then observed under light microscope. The result of this study performed there were the differences of histological structure of the ducks spleen. The capsule thickness had increased along with increasing age, which was 16.0 ± 1.0 µm; 25.3 ± 2.5 µm; and 29.0 ± 1.0 µm. The boundary between the two pulp in the tegal duck 3 months  old more obvious than 1 month or 2 months old. The diameter of white pulp had increased in the oldest duck. It can be concluded that the development of spleen histological structure was more perfect with the  increasing age of tegal duck.