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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Elkawnie MIX : Jurnal Ilmiah Manajemen The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Paediatrica Indonesiana Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Jurnal Kesehatan Prima JPSCR : Journal of Pharmaceutical Science and Clinical Research Indonesian Journal for Health Sciences (IJHS) SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences Action Research Literate (ARL) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Public Health and Preventive Medicine Archive Berita Kedokteran Masyarakat Jurnal Indonesia Sosial Sains Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ASPIRATOR - Journal of Vector-Borne Diseases Studies
Dini Agustina
Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Journal : Jurnal Bioteknologi

 1 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Co-Authors Alfianti, Nita Ali Santosa Anaditya Wahyu Kumudhaningsih Anastasia Citra Purwani Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Aprilya Pratiwi, Nadilla Arief Subhan Aryasatya, Dimas Widyadhana Bhanu Astuti Setyawardani Bagus Dwi Kurniawan Bagus Dwi Kurniawan Bagus Hermansyah Bagus Hermansyah Budi Suharjo Cahyaningrum, Endiningtyas Cholis Abrori, Cholis Cicih Komariah De Rorna, Masrahma Reinataya Denaneer Rahmadatu Desie Dwi Wisudanti Diana C. Mufida Diana Chusna Mufida Diana Chusna Mufida Dina Helianti Dion Krismashogi Dharmawan Dua , Mikhael Dwita Aryadina Rachmawati El Milla, Kardiana Izza Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Enny Suswati Erfan Efendi Erma Sulistyaningsih Fitri, Nadya Eka Gama Wisnu Sanjaya Habib Mustofa Habibbur Rochman Salim Habibi, Ali Hamzah, Salsabilla Maula Zalfa El Hanifa Rizki A.S. Hibatulloh, Muhammad Farhan Himma, Alyatul I Nyoman Semita Iftinan, Karenzha Ika Rahmawati Sutejo Intan Palupi Irawan Fajar Kusuma Jamalullail, Jamalullail Jauhani, Muhammad Afiful Jauhar Firdaus Kirana Nadyatara Kristianningrum Dian Sofiana Kusiyanto Taslim, Fitranda Laila Rizqi Kurniawati Lailatis Shofia Laksmi Indreswari M. Ali Shodikin Mei Liana Wati Miftakhul Huda Muhammad A. Shodikin Muhammad Ali Shodikin Muhammad Dimas Arya Candra Permana Mukhamad Najib Napitupulu , Frengki Nastiti Bekti Utami Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Noorhamdani AS Noorhamdani AS Nugraha, Bima Setia Sandya Nurul Indah Saffanah Nurul Indah Saffanah Nurus Saffana Yulianto Pralampita, Pulong Wijang Primadana, Aditya Putra Uta, Diego Rafi Putra, Muhamad Dwi Eka Putra, Yudha Ananta Khaerul Ramadhani, Alfiyah Regina Finka Rinda Yanuarisa Rony Prasetyo Rosita Dewi Rustono - Ryan Ravi Is Syahputra S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Sakinah, Elly N Salsabila, Yuna Annisa Salsabilla Maula Zalfa El Hamzah Saputra, Antonius Dwi Sarah Marsa Tamimi Semita, I Nyoman septa surya wahyudi, septa surya Setyawardani, Astuti Sheilla Rachmania, Sheilla Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Siti Zulaikha Sumarno Retoprawiro Supangat Supangat Syahputri, Siti Ananda Hardita Tristianti, Farmitalia Nisa Udi Rusadi Ulfa Elfiah Ulin Nuha Jazmin Widaningsih, Titi Widhiasari Normaningtyas Wiwien Sugih Utami Yudha Nurdian Yuli Hermansyah Yuli Rahmantia, Vania Yuna Annisa Salsabila Yunita Armiyanti